6RJD

Cryo-EM structure of St1Cas9-sgRNA-tDNA59-ntPAM complex.

Functional Information from GO Data

Chain	GOid	namespace	contents
C	0003676	molecular_function	nucleic acid binding
C	0003677	molecular_function	DNA binding
C	0003723	molecular_function	RNA binding
C	0004519	molecular_function	endonuclease activity
C	0043571	biological_process	maintenance of CRISPR repeat elements
C	0046872	molecular_function	metal ion binding
C	0051607	biological_process	defense response to virus

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: For RuvC-like nuclease domain => ECO:0000255|HAMAP-Rule:MF_01480

Chain	Residue	Details
C	ASP9

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton acceptor for HNH nuclease domain => ECO:0000255|HAMAP-Rule:MF_01480

Chain	Residue	Details
C	HIS599

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site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01480

Chain	Residue	Details
C	ASP9
C	GLU509
C	GLU513
C	HIS738

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