6RE9

Cryo-EM structure of Polytomella F-ATP synthase, Rotary substate 2D, monomer-masked refinement

Functional Information from GO Data

Chain	GOid	namespace	contents
0	0016020	cellular_component	membrane
1	0005739	cellular_component	mitochondrion
8	0016020	cellular_component	membrane
9	0016020	cellular_component	membrane
A	0008289	molecular_function	lipid binding
A	0015078	molecular_function	proton transmembrane transporter activity
A	0015986	biological_process	proton motive force-driven ATP synthesis
A	0016020	cellular_component	membrane
A	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
A	0045263	cellular_component	proton-transporting ATP synthase complex, coupling factor F(o)
A	1902600	biological_process	proton transmembrane transport
B	0008289	molecular_function	lipid binding
B	0015078	molecular_function	proton transmembrane transporter activity
B	0015986	biological_process	proton motive force-driven ATP synthesis
B	0016020	cellular_component	membrane
B	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
B	0045263	cellular_component	proton-transporting ATP synthase complex, coupling factor F(o)
B	1902600	biological_process	proton transmembrane transport
C	0008289	molecular_function	lipid binding
C	0015078	molecular_function	proton transmembrane transporter activity
C	0015986	biological_process	proton motive force-driven ATP synthesis
C	0016020	cellular_component	membrane
C	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
C	0045263	cellular_component	proton-transporting ATP synthase complex, coupling factor F(o)
C	1902600	biological_process	proton transmembrane transport
D	0008289	molecular_function	lipid binding
D	0015078	molecular_function	proton transmembrane transporter activity
D	0015986	biological_process	proton motive force-driven ATP synthesis
D	0016020	cellular_component	membrane
D	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
D	0045263	cellular_component	proton-transporting ATP synthase complex, coupling factor F(o)
D	1902600	biological_process	proton transmembrane transport
E	0008289	molecular_function	lipid binding
E	0015078	molecular_function	proton transmembrane transporter activity
E	0015986	biological_process	proton motive force-driven ATP synthesis
E	0016020	cellular_component	membrane
E	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
E	0045263	cellular_component	proton-transporting ATP synthase complex, coupling factor F(o)
E	1902600	biological_process	proton transmembrane transport
F	0008289	molecular_function	lipid binding
F	0015078	molecular_function	proton transmembrane transporter activity
F	0015986	biological_process	proton motive force-driven ATP synthesis
F	0016020	cellular_component	membrane
F	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
F	0045263	cellular_component	proton-transporting ATP synthase complex, coupling factor F(o)
F	1902600	biological_process	proton transmembrane transport
G	0008289	molecular_function	lipid binding
G	0015078	molecular_function	proton transmembrane transporter activity
G	0015986	biological_process	proton motive force-driven ATP synthesis
G	0016020	cellular_component	membrane
G	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
G	0045263	cellular_component	proton-transporting ATP synthase complex, coupling factor F(o)
G	1902600	biological_process	proton transmembrane transport
H	0008289	molecular_function	lipid binding
H	0015078	molecular_function	proton transmembrane transporter activity
H	0015986	biological_process	proton motive force-driven ATP synthesis
H	0016020	cellular_component	membrane
H	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
H	0045263	cellular_component	proton-transporting ATP synthase complex, coupling factor F(o)
H	1902600	biological_process	proton transmembrane transport
I	0008289	molecular_function	lipid binding
I	0015078	molecular_function	proton transmembrane transporter activity
I	0015986	biological_process	proton motive force-driven ATP synthesis
I	0016020	cellular_component	membrane
I	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
I	0045263	cellular_component	proton-transporting ATP synthase complex, coupling factor F(o)
I	1902600	biological_process	proton transmembrane transport
J	0008289	molecular_function	lipid binding
J	0015078	molecular_function	proton transmembrane transporter activity
J	0015986	biological_process	proton motive force-driven ATP synthesis
J	0016020	cellular_component	membrane
J	0033177	cellular_component	proton-transporting two-sector ATPase complex, proton-transporting domain
J	0045263	cellular_component	proton-transporting ATP synthase complex, coupling factor F(o)
J	1902600	biological_process	proton transmembrane transport
M	0006754	biological_process	ATP biosynthetic process
M	0015078	molecular_function	proton transmembrane transporter activity
M	0015986	biological_process	proton motive force-driven ATP synthesis
M	0016020	cellular_component	membrane
M	0045259	cellular_component	proton-transporting ATP synthase complex
M	0045263	cellular_component	proton-transporting ATP synthase complex, coupling factor F(o)
M	0046933	molecular_function	proton-transporting ATP synthase activity, rotational mechanism
M	1902600	biological_process	proton transmembrane transport
P	0006754	biological_process	ATP biosynthetic process
P	0015986	biological_process	proton motive force-driven ATP synthesis
P	0016020	cellular_component	membrane
P	0046933	molecular_function	proton-transporting ATP synthase activity, rotational mechanism
P	1902600	biological_process	proton transmembrane transport
Q	0005743	cellular_component	mitochondrial inner membrane
Q	0015986	biological_process	proton motive force-driven ATP synthesis
Q	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
Q	0045261	cellular_component	proton-transporting ATP synthase complex, catalytic core F(1)
Q	0046933	molecular_function	proton-transporting ATP synthase activity, rotational mechanism
R	0005739	cellular_component	mitochondrion
R	0005743	cellular_component	mitochondrial inner membrane
R	0015986	biological_process	proton motive force-driven ATP synthesis
R	0045261	cellular_component	proton-transporting ATP synthase complex, catalytic core F(1)
R	0046933	molecular_function	proton-transporting ATP synthase activity, rotational mechanism
R	1902600	biological_process	proton transmembrane transport
S	0015986	biological_process	proton motive force-driven ATP synthesis
S	0045261	cellular_component	proton-transporting ATP synthase complex, catalytic core F(1)
S	0046933	molecular_function	proton-transporting ATP synthase activity, rotational mechanism
T	0005524	molecular_function	ATP binding
T	0005743	cellular_component	mitochondrial inner membrane
T	0006754	biological_process	ATP biosynthetic process
T	0015986	biological_process	proton motive force-driven ATP synthesis
T	0016787	molecular_function	hydrolase activity
T	0032559	molecular_function	adenyl ribonucleotide binding
T	0043531	molecular_function	ADP binding
T	0045261	cellular_component	proton-transporting ATP synthase complex, catalytic core F(1)
T	0046034	biological_process	ATP metabolic process
T	0046933	molecular_function	proton-transporting ATP synthase activity, rotational mechanism
T	1902600	biological_process	proton transmembrane transport
U	0005524	molecular_function	ATP binding
U	0005743	cellular_component	mitochondrial inner membrane
U	0006754	biological_process	ATP biosynthetic process
U	0015986	biological_process	proton motive force-driven ATP synthesis
U	0016787	molecular_function	hydrolase activity
U	0032559	molecular_function	adenyl ribonucleotide binding
U	0043531	molecular_function	ADP binding
U	0045261	cellular_component	proton-transporting ATP synthase complex, catalytic core F(1)
U	0046034	biological_process	ATP metabolic process
U	0046933	molecular_function	proton-transporting ATP synthase activity, rotational mechanism
U	1902600	biological_process	proton transmembrane transport
V	0005524	molecular_function	ATP binding
V	0005743	cellular_component	mitochondrial inner membrane
V	0006754	biological_process	ATP biosynthetic process
V	0015986	biological_process	proton motive force-driven ATP synthesis
V	0016787	molecular_function	hydrolase activity
V	0032559	molecular_function	adenyl ribonucleotide binding
V	0043531	molecular_function	ADP binding
V	0045261	cellular_component	proton-transporting ATP synthase complex, catalytic core F(1)
V	0046034	biological_process	ATP metabolic process
V	0046933	molecular_function	proton-transporting ATP synthase activity, rotational mechanism
V	1902600	biological_process	proton transmembrane transport
X	0005524	molecular_function	ATP binding
X	0005739	cellular_component	mitochondrion
X	0006754	biological_process	ATP biosynthetic process
X	0015986	biological_process	proton motive force-driven ATP synthesis
X	0016020	cellular_component	membrane
X	0016787	molecular_function	hydrolase activity
X	0016887	molecular_function	ATP hydrolysis activity
X	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
X	0045261	cellular_component	proton-transporting ATP synthase complex, catalytic core F(1)
X	0046034	biological_process	ATP metabolic process
X	0046933	molecular_function	proton-transporting ATP synthase activity, rotational mechanism
X	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
X	1902600	biological_process	proton transmembrane transport
Y	0005524	molecular_function	ATP binding
Y	0005739	cellular_component	mitochondrion
Y	0006754	biological_process	ATP biosynthetic process
Y	0015986	biological_process	proton motive force-driven ATP synthesis
Y	0016020	cellular_component	membrane
Y	0016787	molecular_function	hydrolase activity
Y	0016887	molecular_function	ATP hydrolysis activity
Y	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
Y	0045261	cellular_component	proton-transporting ATP synthase complex, catalytic core F(1)
Y	0046034	biological_process	ATP metabolic process
Y	0046933	molecular_function	proton-transporting ATP synthase activity, rotational mechanism
Y	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
Y	1902600	biological_process	proton transmembrane transport
Z	0005524	molecular_function	ATP binding
Z	0005739	cellular_component	mitochondrion
Z	0006754	biological_process	ATP biosynthetic process
Z	0015986	biological_process	proton motive force-driven ATP synthesis
Z	0016020	cellular_component	membrane
Z	0016787	molecular_function	hydrolase activity
Z	0016887	molecular_function	ATP hydrolysis activity
Z	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
Z	0045261	cellular_component	proton-transporting ATP synthase complex, catalytic core F(1)
Z	0046034	biological_process	ATP metabolic process
Z	0046933	molecular_function	proton-transporting ATP synthase activity, rotational mechanism
Z	0046961	molecular_function	proton-transporting ATPase activity, rotational mechanism
Z	1902600	biological_process	proton transmembrane transport

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	binding site for residue ZN M 600

Chain	Residue
M	HIS248
M	HIS252

---

site_id	AC2
Number of Residues	13
Details	binding site for residue ATP T 1001

Chain	Residue
T	ARG418
T	GLN486
T	GLN488
T	MG1002
X	ARG385
X	ASN388
T	ARG227
T	GLN228
T	GLY230
T	LYS231
T	THR232
T	ALA233
T	PHE413

---

site_id	AC3
Number of Residues	3
Details	binding site for residue MG T 1002

Chain	Residue
T	THR232
T	ASP325
T	ATP1001

---

site_id	AC4
Number of Residues	14
Details	binding site for residue ATP U 1001

Chain	Residue
U	GLN228
U	THR229
U	GLY230
U	LYS231
U	THR232
U	ALA233
U	PHE413
U	ARG418
U	PRO419
U	GLN486
U	GLN488
U	MG1002
Z	ARG385
Z	TYR397

---

site_id	AC5
Number of Residues	2
Details	binding site for residue MG U 1002

Chain	Residue
U	THR232
U	ATP1001

---

site_id	AC6
Number of Residues	14
Details	binding site for residue ATP V 1001

Chain	Residue
V	ARG227
V	GLN228
V	THR229
V	GLY230
V	LYS231
V	THR232
V	ALA233
V	PHE413
V	ARG418
V	GLN486
V	LYS487
V	GLN488
V	MG1002
Y	TYR397

---

site_id	AC7
Number of Residues	2
Details	binding site for residue MG V 1002

Chain	Residue
V	THR232
V	ATP1001

---

site_id	AC8
Number of Residues	10
Details	binding site for residue ADP Y 601

Chain	Residue
T	VAL427
Y	GLY186
Y	GLY188
Y	LYS189
Y	THR190
Y	VAL191
Y	TYR374
Y	PHE447
Y	PHE453
Y	MG602

---

site_id	AC9
Number of Residues	4
Details	binding site for residue MG Y 602

Chain	Residue
Y	THR190
Y	GLU215
Y	ARG216
Y	ADP601

---

site_id	AD1
Number of Residues	11
Details	binding site for residue ADP Z 601

Chain	Residue
V	ARG429
Z	GLY186
Z	VAL187
Z	GLY188
Z	LYS189
Z	THR190
Z	VAL191
Z	TYR374
Z	PHE447
Z	ALA450
Z	MG602

---

site_id	AD2
Number of Residues	5
Details	binding site for residue MG Z 602

Chain	Residue
Z	THR190
Z	GLU215
Z	ARG216
Z	GLU219
Z	ADP601

---

Functional Information from PROSITE/UniProt

site_id	PS00152
Number of Residues	10
Details	ATPASE_ALPHA_BETA ATP synthase alpha and beta subunits signature. PALNVGLSVS

Chain	Residue	Details
U	PRO419-SER428
X	PRO375-SER384

---

site_id	PS00153
Number of Residues	14
Details	ATPASE_GAMMA ATP synthase gamma subunit signature. ITtEliEiiaGAsA

Chain	Residue	Details
S	ILE300-ALA313

---

site_id	PS00389
Number of Residues	20
Details	ATPASE_DELTA ATP synthase delta (OSCP) subunit signature. LvMqekIDkKLlGGFVIEfS

Chain	Residue	Details
P	LEU184-SER203

---

site_id	PS00449
Number of Residues	10
Details	ATPASE_A ATP synthase a subunit signature. SLGVRLWVNM

Chain	Residue	Details
M	SER235-MET244

---

site_id	PS00605
Number of Residues	22
Details	ATPASE_C ATP synthase c subunit signature. ARNPniakqLvGyaLLgfaLtE

Chain	Residue	Details
A	ALA90-GLU111

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