6QGR
The F420-reducing [NiFe] hydrogenase complex from Methanosarcina barkeri at the Nia-S state
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0008901 | molecular_function | ferredoxin hydrogenase activity |
| A | 0016151 | molecular_function | nickel cation binding |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0050454 | molecular_function | coenzyme F420 hydrogenase activity |
| A | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| A | 0051536 | molecular_function | iron-sulfur cluster binding |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0016151 | molecular_function | nickel cation binding |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0050454 | molecular_function | coenzyme F420 hydrogenase activity |
| B | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| B | 0051536 | molecular_function | iron-sulfur cluster binding |
| B | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
| B | 0052592 | molecular_function | oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor |
| G | 0016151 | molecular_function | nickel cation binding |
| G | 0016491 | molecular_function | oxidoreductase activity |
| G | 0046872 | molecular_function | metal ion binding |
| G | 0050454 | molecular_function | coenzyme F420 hydrogenase activity |
| G | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| G | 0051536 | molecular_function | iron-sulfur cluster binding |
| G | 0051537 | molecular_function | 2 iron, 2 sulfur cluster binding |
| G | 0051539 | molecular_function | 4 iron, 4 sulfur cluster binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 10 |
| Details | binding site for residue SF4 G 301 |
| Chain | Residue |
| A | ARG61 |
| G | CYS145 |
| A | HIS162 |
| G | CYS31 |
| G | CYS34 |
| G | GLU76 |
| G | SER105 |
| G | CYS106 |
| G | ASN111 |
| G | GLY144 |
| site_id | AC2 |
| Number of Residues | 9 |
| Details | binding site for residue SF4 G 302 |
| Chain | Residue |
| G | CYS191 |
| G | PHE192 |
| G | CYS215 |
| G | PRO216 |
| G | CYS234 |
| G | ILE235 |
| G | CYS237 |
| G | GLY238 |
| G | CYS240 |
| site_id | AC3 |
| Number of Residues | 9 |
| Details | binding site for residue SF4 G 303 |
| Chain | Residue |
| G | CYS205 |
| G | MET206 |
| G | GLY207 |
| G | CYS208 |
| G | GLY209 |
| G | THR210 |
| G | CYS211 |
| G | CYS244 |
| G | PRO245 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue FES G 304 |
| Chain | Residue |
| G | CYS191 |
| G | CYS193 |
| G | ARG231 |
| G | ARG231 |
| G | LYS236 |
| site_id | AC5 |
| Number of Residues | 9 |
| Details | binding site for residue 144 G 305 |
| Chain | Residue |
| A | PRO9 |
| A | HIS16 |
| G | TYR57 |
| G | ARG65 |
| G | HIS66 |
| G | GLU85 |
| G | ASP89 |
| G | HOH478 |
| G | HOH523 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | binding site for residue BU3 G 306 |
| Chain | Residue |
| B | GLN117 |
| B | PRO127 |
| G | ARG153 |
| G | SER261 |
| G | HOH497 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | binding site for residue BU3 G 307 |
| Chain | Residue |
| B | SER87 |
| G | THR221 |
| G | LEU222 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | binding site for residue MPD G 308 |
| Chain | Residue |
| A | PHE129 |
| A | MET139 |
| G | ILE46 |
| G | LEU49 |
| G | HOH464 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue BU3 G 309 |
| Chain | Residue |
| A | HIS173 |
| B | SER87 |
| B | GLU91 |
| G | ALA212 |
| G | HIS218 |
| G | HOH515 |
| site_id | AD1 |
| Number of Residues | 13 |
| Details | binding site for residue NFU A 501 |
| Chain | Residue |
| A | CYS63 |
| A | CYS66 |
| A | ALA69 |
| A | HIS70 |
| A | ALA378 |
| A | PRO379 |
| A | ARG380 |
| A | ASN383 |
| A | VAL401 |
| A | PRO402 |
| A | THR403 |
| A | CYS432 |
| A | CYS435 |
| site_id | AD2 |
| Number of Residues | 6 |
| Details | binding site for residue FE A 502 |
| Chain | Residue |
| A | GLU44 |
| A | MET399 |
| A | HIS438 |
| A | HOH609 |
| A | HOH619 |
| G | HOH415 |
| site_id | AD3 |
| Number of Residues | 6 |
| Details | binding site for residue MG A 503 |
| Chain | Residue |
| A | ASN322 |
| A | ASN322 |
| A | ASN322 |
| A | HOH720 |
| A | HOH720 |
| A | HOH720 |
| site_id | AD4 |
| Number of Residues | 3 |
| Details | binding site for residue MPD A 504 |
| Chain | Residue |
| A | ARG178 |
| A | GLN181 |
| A | HOH621 |
| site_id | AD5 |
| Number of Residues | 6 |
| Details | binding site for residue 144 A 505 |
| Chain | Residue |
| A | ASN322 |
| A | ASN322 |
| A | ASN322 |
| A | PHE323 |
| A | PHE323 |
| A | PHE323 |
| site_id | AD6 |
| Number of Residues | 4 |
| Details | binding site for residue MPD A 506 |
| Chain | Residue |
| A | ALA80 |
| A | SER81 |
| A | ILE82 |
| A | SER370 |
| site_id | AD7 |
| Number of Residues | 3 |
| Details | binding site for residue MPD A 507 |
| Chain | Residue |
| A | MET371 |
| A | HOH660 |
| A | HOH770 |
| site_id | AD8 |
| Number of Residues | 2 |
| Details | binding site for residue BU3 A 508 |
| Chain | Residue |
| A | ARG388 |
| A | TRP396 |
| site_id | AD9 |
| Number of Residues | 4 |
| Details | binding site for residue MPD A 509 |
| Chain | Residue |
| A | THR251 |
| A | THR319 |
| A | PHE320 |
| A | HOH909 |
| site_id | AE1 |
| Number of Residues | 3 |
| Details | binding site for residue BU3 A 510 |
| Chain | Residue |
| A | ASP368 |
| A | SER370 |
| A | HOH685 |
| site_id | AE2 |
| Number of Residues | 5 |
| Details | binding site for residue BU3 B 301 |
| Chain | Residue |
| B | GLN117 |
| B | HOH468 |
| B | HOH469 |
| G | GLU258 |
| G | SER261 |
| site_id | AE3 |
| Number of Residues | 34 |
| Details | binding site for residue FAD B 302 |
| Chain | Residue |
| B | ALA27 |
| B | GLN28 |
| B | ASP29 |
| B | GLY30 |
| B | GLY31 |
| B | ILE32 |
| B | ALA33 |
| B | THR34 |
| B | VAL51 |
| B | ALA52 |
| B | ALA75 |
| B | ARG76 |
| B | GLY77 |
| B | THR78 |
| B | ARG79 |
| B | ASN81 |
| B | SER83 |
| B | THR104 |
| B | GLN109 |
| B | LEU136 |
| B | PHE137 |
| B | CYS138 |
| B | MET139 |
| B | GLU140 |
| B | ASN141 |
| B | TYR203 |
| B | GLY213 |
| B | SER214 |
| B | VAL215 |
| B | HOH421 |
| B | HOH426 |
| B | HOH434 |
| B | HOH460 |
| B | HOH479 |
| site_id | AE4 |
| Number of Residues | 11 |
| Details | binding site for residue SF4 B 303 |
| Chain | Residue |
| B | CYS107 |
| B | CYS108 |
| B | CYS138 |
| B | MET139 |
| B | GLU140 |
| B | ASN141 |
| B | CYS197 |
| B | CYS200 |
| B | LYS266 |
| B | HOH550 |
| G | ARG246 |
| site_id | AE5 |
| Number of Residues | 3 |
| Details | binding site for residue BU3 B 304 |
| Chain | Residue |
| B | ASP125 |
| B | HOH528 |
| G | GLU150 |
| site_id | AE6 |
| Number of Residues | 5 |
| Details | binding site for residue BU3 B 305 |
| Chain | Residue |
| B | MET139 |
| B | ILE167 |
| B | LYS169 |
| B | GLY170 |
| B | LEU186 |
| site_id | AE7 |
| Number of Residues | 3 |
| Details | binding site for residue BU3 B 306 |
| Chain | Residue |
| B | GLU54 |
| B | TRP59 |
| B | HOH455 |
Functional Information from PROSITE/UniProt
| site_id | PS00198 |
| Number of Residues | 12 |
| Details | 4FE4S_FER_1 4Fe-4S ferredoxin-type iron-sulfur binding region signature. CmGCGtCAaSCP |
| Chain | Residue | Details |
| G | CYS205-PRO216 | |
| G | CYS234-PRO245 |
| site_id | PS00507 |
| Number of Residues | 26 |
| Details | NI_HGENASE_L_1 Nickel-dependent hydrogenases large subunit signature 1. RGIEklaigktmeqvpkiaSRvCGIC |
| Chain | Residue | Details |
| A | ARG41-CYS66 |
| site_id | PS00508 |
| Number of Residues | 10 |
| Details | NI_HGENASE_L_2 Nickel-dependent hydrogenases large subunit signature 2. YDPCVSCat.H |
| Chain | Residue | Details |
| A | TYR429-HIS438 |
