Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6PXK

3.65 Angstroms resolution structure of HslU with an axial-channel plug

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004176	molecular_function	ATP-dependent peptidase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006508	biological_process	proteolysis
A	0008233	molecular_function	peptidase activity
A	0009376	cellular_component	HslUV protease complex
A	0009408	biological_process	response to heat
A	0016020	cellular_component	membrane
A	0016887	molecular_function	ATP hydrolysis activity
A	0019904	molecular_function	protein domain specific binding
A	0030164	biological_process	protein denaturation
A	0034605	biological_process	cellular response to heat
A	0036402	molecular_function	proteasome-activating activity
A	0042802	molecular_function	identical protein binding
A	0043335	biological_process	protein unfolding
A	0051603	biological_process	proteolysis involved in protein catabolic process
A	1901800	biological_process	positive regulation of proteasomal protein catabolic process
B	0000287	molecular_function	magnesium ion binding
B	0004176	molecular_function	ATP-dependent peptidase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006508	biological_process	proteolysis
B	0008233	molecular_function	peptidase activity
B	0009376	cellular_component	HslUV protease complex
B	0009408	biological_process	response to heat
B	0016020	cellular_component	membrane
B	0016887	molecular_function	ATP hydrolysis activity
B	0019904	molecular_function	protein domain specific binding
B	0030164	biological_process	protein denaturation
B	0034605	biological_process	cellular response to heat
B	0036402	molecular_function	proteasome-activating activity
B	0042802	molecular_function	identical protein binding
B	0043335	biological_process	protein unfolding
B	0051603	biological_process	proteolysis involved in protein catabolic process
B	1901800	biological_process	positive regulation of proteasomal protein catabolic process
C	0000287	molecular_function	magnesium ion binding
C	0004176	molecular_function	ATP-dependent peptidase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006508	biological_process	proteolysis
C	0008233	molecular_function	peptidase activity
C	0009376	cellular_component	HslUV protease complex
C	0009408	biological_process	response to heat
C	0016020	cellular_component	membrane
C	0016887	molecular_function	ATP hydrolysis activity
C	0019904	molecular_function	protein domain specific binding
C	0030164	biological_process	protein denaturation
C	0034605	biological_process	cellular response to heat
C	0036402	molecular_function	proteasome-activating activity
C	0042802	molecular_function	identical protein binding
C	0043335	biological_process	protein unfolding
C	0051603	biological_process	proteolysis involved in protein catabolic process
C	1901800	biological_process	positive regulation of proteasomal protein catabolic process
D	0000287	molecular_function	magnesium ion binding
D	0004176	molecular_function	ATP-dependent peptidase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006508	biological_process	proteolysis
D	0008233	molecular_function	peptidase activity
D	0009376	cellular_component	HslUV protease complex
D	0009408	biological_process	response to heat
D	0016020	cellular_component	membrane
D	0016887	molecular_function	ATP hydrolysis activity
D	0019904	molecular_function	protein domain specific binding
D	0030164	biological_process	protein denaturation
D	0034605	biological_process	cellular response to heat
D	0036402	molecular_function	proteasome-activating activity
D	0042802	molecular_function	identical protein binding
D	0043335	biological_process	protein unfolding
D	0051603	biological_process	proteolysis involved in protein catabolic process
D	1901800	biological_process	positive regulation of proteasomal protein catabolic process
E	0000287	molecular_function	magnesium ion binding
E	0004176	molecular_function	ATP-dependent peptidase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006508	biological_process	proteolysis
E	0008233	molecular_function	peptidase activity
E	0009376	cellular_component	HslUV protease complex
E	0009408	biological_process	response to heat
E	0016020	cellular_component	membrane
E	0016887	molecular_function	ATP hydrolysis activity
E	0019904	molecular_function	protein domain specific binding
E	0030164	biological_process	protein denaturation
E	0034605	biological_process	cellular response to heat
E	0036402	molecular_function	proteasome-activating activity
E	0042802	molecular_function	identical protein binding
E	0043335	biological_process	protein unfolding
E	0051603	biological_process	proteolysis involved in protein catabolic process
E	1901800	biological_process	positive regulation of proteasomal protein catabolic process
F	0000287	molecular_function	magnesium ion binding
F	0004176	molecular_function	ATP-dependent peptidase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006508	biological_process	proteolysis
F	0008233	molecular_function	peptidase activity
F	0009376	cellular_component	HslUV protease complex
F	0009408	biological_process	response to heat
F	0016020	cellular_component	membrane
F	0016887	molecular_function	ATP hydrolysis activity
F	0019904	molecular_function	protein domain specific binding
F	0030164	biological_process	protein denaturation
F	0034605	biological_process	cellular response to heat
F	0036402	molecular_function	proteasome-activating activity
F	0042802	molecular_function	identical protein binding
F	0043335	biological_process	protein unfolding
F	0051603	biological_process	proteolysis involved in protein catabolic process
F	1901800	biological_process	positive regulation of proteasomal protein catabolic process
G	0000287	molecular_function	magnesium ion binding
G	0004176	molecular_function	ATP-dependent peptidase activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006508	biological_process	proteolysis
G	0008233	molecular_function	peptidase activity
G	0009376	cellular_component	HslUV protease complex
G	0009408	biological_process	response to heat
G	0016020	cellular_component	membrane
G	0016887	molecular_function	ATP hydrolysis activity
G	0019904	molecular_function	protein domain specific binding
G	0030164	biological_process	protein denaturation
G	0034605	biological_process	cellular response to heat
G	0036402	molecular_function	proteasome-activating activity
G	0042802	molecular_function	identical protein binding
G	0043335	biological_process	protein unfolding
G	0051603	biological_process	proteolysis involved in protein catabolic process
G	1901800	biological_process	positive regulation of proteasomal protein catabolic process
H	0000287	molecular_function	magnesium ion binding
H	0004176	molecular_function	ATP-dependent peptidase activity
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006508	biological_process	proteolysis
H	0008233	molecular_function	peptidase activity
H	0009376	cellular_component	HslUV protease complex
H	0009408	biological_process	response to heat
H	0016020	cellular_component	membrane
H	0016887	molecular_function	ATP hydrolysis activity
H	0019904	molecular_function	protein domain specific binding
H	0030164	biological_process	protein denaturation
H	0034605	biological_process	cellular response to heat
H	0036402	molecular_function	proteasome-activating activity
H	0042802	molecular_function	identical protein binding
H	0043335	biological_process	protein unfolding
H	0051603	biological_process	proteolysis involved in protein catabolic process
H	1901800	biological_process	positive regulation of proteasomal protein catabolic process
I	0000287	molecular_function	magnesium ion binding
I	0004176	molecular_function	ATP-dependent peptidase activity
I	0005515	molecular_function	protein binding
I	0005524	molecular_function	ATP binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006508	biological_process	proteolysis
I	0008233	molecular_function	peptidase activity
I	0009376	cellular_component	HslUV protease complex
I	0009408	biological_process	response to heat
I	0016020	cellular_component	membrane
I	0016887	molecular_function	ATP hydrolysis activity
I	0019904	molecular_function	protein domain specific binding
I	0030164	biological_process	protein denaturation
I	0034605	biological_process	cellular response to heat
I	0036402	molecular_function	proteasome-activating activity
I	0042802	molecular_function	identical protein binding
I	0043335	biological_process	protein unfolding
I	0051603	biological_process	proteolysis involved in protein catabolic process
I	1901800	biological_process	positive regulation of proteasomal protein catabolic process
J	0000287	molecular_function	magnesium ion binding
J	0004176	molecular_function	ATP-dependent peptidase activity
J	0005515	molecular_function	protein binding
J	0005524	molecular_function	ATP binding
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006508	biological_process	proteolysis
J	0008233	molecular_function	peptidase activity
J	0009376	cellular_component	HslUV protease complex
J	0009408	biological_process	response to heat
J	0016020	cellular_component	membrane
J	0016887	molecular_function	ATP hydrolysis activity
J	0019904	molecular_function	protein domain specific binding
J	0030164	biological_process	protein denaturation
J	0034605	biological_process	cellular response to heat
J	0036402	molecular_function	proteasome-activating activity
J	0042802	molecular_function	identical protein binding
J	0043335	biological_process	protein unfolding
J	0051603	biological_process	proteolysis involved in protein catabolic process
J	1901800	biological_process	positive regulation of proteasomal protein catabolic process
K	0000287	molecular_function	magnesium ion binding
K	0004176	molecular_function	ATP-dependent peptidase activity
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006508	biological_process	proteolysis
K	0008233	molecular_function	peptidase activity
K	0009376	cellular_component	HslUV protease complex
K	0009408	biological_process	response to heat
K	0016020	cellular_component	membrane
K	0016887	molecular_function	ATP hydrolysis activity
K	0019904	molecular_function	protein domain specific binding
K	0030164	biological_process	protein denaturation
K	0034605	biological_process	cellular response to heat
K	0036402	molecular_function	proteasome-activating activity
K	0042802	molecular_function	identical protein binding
K	0043335	biological_process	protein unfolding
K	0051603	biological_process	proteolysis involved in protein catabolic process
K	1901800	biological_process	positive regulation of proteasomal protein catabolic process
L	0000287	molecular_function	magnesium ion binding
L	0004176	molecular_function	ATP-dependent peptidase activity
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0006508	biological_process	proteolysis
L	0008233	molecular_function	peptidase activity
L	0009376	cellular_component	HslUV protease complex
L	0009408	biological_process	response to heat
L	0016020	cellular_component	membrane
L	0016887	molecular_function	ATP hydrolysis activity
L	0019904	molecular_function	protein domain specific binding
L	0030164	biological_process	protein denaturation
L	0034605	biological_process	cellular response to heat
L	0036402	molecular_function	proteasome-activating activity
L	0042802	molecular_function	identical protein binding
L	0043335	biological_process	protein unfolding
L	0051603	biological_process	proteolysis involved in protein catabolic process
L	1901800	biological_process	positive regulation of proteasomal protein catabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	14
Details	binding site for residue ADP G 501

Chain	Residue
G	HIS16
G	GLU65
G	LEU335
G	ILE343
G	ALA392
G	ARG393
G	ILE17
G	ILE18
G	THR59
G	GLY60
G	VAL61
G	GLY62
G	LYS63
G	THR64

site_id	AC2
Number of Residues	4
Details	binding site for residue SO4 G 502

Chain	Residue
G	THR369
G	SER371
G	ASP421
G	ALA422

site_id	AC3
Number of Residues	15
Details	binding site for residue ADP H 501

Chain	Residue
H	HIS16
H	ILE17
H	ILE18
H	PRO58
H	THR59
H	GLY60
H	VAL61
H	GLY62
H	LYS63
H	THR64
H	GLU65
H	ILE343
H	ALA392
H	ARG393
H	HIS396

site_id	AC4
Number of Residues	4
Details	binding site for residue SO4 H 502

Chain	Residue
H	ARG37
H	ARG45
H	HIS46
H	HIS301

site_id	AC5
Number of Residues	4
Details	binding site for residue SO4 H 503

Chain	Residue
H	THR369
H	SER371
H	ASP421
H	ALA422

site_id	AC6
Number of Residues	11
Details	binding site for residue ADP I 501

Chain	Residue
I	ILE18
I	THR59
I	GLY60
I	VAL61
I	GLY62
I	LYS63
I	THR64
I	GLU65
I	ILE343
I	ALA392
I	ARG393

site_id	AC7
Number of Residues	2
Details	binding site for residue SO4 I 502

Chain	Residue
I	HIS46
I	HIS301

site_id	AC8
Number of Residues	4
Details	binding site for residue SO4 I 503

Chain	Residue
I	THR369
I	SER371
I	ASP421
I	ALA422

site_id	AC9
Number of Residues	14
Details	binding site for residue ADP J 501

Chain	Residue
J	HIS16
J	ILE17
J	ILE18
J	THR59
J	GLY60
J	VAL61
J	GLY62
J	LYS63
J	THR64
J	GLU65
J	ILE343
J	ALA392
J	ARG393
J	HIS396

site_id	AD1
Number of Residues	2
Details	binding site for residue SO4 J 502

Chain	Residue
J	LYS298
J	ASP300

site_id	AD2
Number of Residues	1
Details	binding site for residue SO4 J 503

Chain	Residue
J	LYS72

site_id	AD3
Number of Residues	13
Details	binding site for residue ADP K 501

Chain	Residue
K	HIS16
K	ILE17
K	ILE18
K	THR59
K	GLY60
K	VAL61
K	GLY62
K	LYS63
K	THR64
K	GLU65
K	ALA392
K	ARG393
K	HIS396

site_id	AD4
Number of Residues	2
Details	binding site for residue SO4 K 502

Chain	Residue
K	ARG37
K	HIS46

site_id	AD5
Number of Residues	4
Details	binding site for residue SO4 K 503

Chain	Residue
K	THR369
K	SER371
K	ASP421
K	ALA422

site_id	AD6
Number of Residues	14
Details	binding site for residue ADP L 501

Chain	Residue
L	VAL61
L	GLY62
L	LYS63
L	THR64
L	GLU65
L	ILE343
L	ALA392
L	ARG393
L	HIS396
L	HIS16
L	ILE17
L	ILE18
L	THR59
L	GLY60

site_id	AD7
Number of Residues	2
Details	binding site for residue SO4 L 502

Chain	Residue
L	ARG7
L	ARG25

site_id	AD8
Number of Residues	14
Details	binding site for residue ADP A 501

Chain	Residue
A	HIS16
A	ILE17
A	ILE18
A	THR59
A	GLY60
A	VAL61
A	GLY62
A	LYS63
A	THR64
A	GLU65
A	LEU335
A	ILE343
A	ALA392
A	ARG393

site_id	AD9
Number of Residues	4
Details	binding site for residue SO4 A 502

Chain	Residue
A	THR369
A	SER371
A	ASP421
A	ALA422

site_id	AE1
Number of Residues	13
Details	binding site for residue ADP B 501

Chain	Residue
B	HIS16
B	ILE17
B	ILE18
B	THR59
B	GLY60
B	VAL61
B	GLY62
B	LYS63
B	THR64
B	GLU65
B	ILE343
B	ALA392
B	ARG393

site_id	AE2
Number of Residues	4
Details	binding site for residue SO4 B 502

Chain	Residue
B	ARG37
B	HIS46
B	GLN249
B	HIS301

site_id	AE3
Number of Residues	14
Details	binding site for residue ADP C 501

Chain	Residue
C	HIS16
C	ILE17
C	ILE18
C	THR59
C	GLY60
C	VAL61
C	GLY62
C	LYS63
C	THR64
C	GLU65
C	ILE343
C	ALA392
C	ARG393
C	HIS396

site_id	AE4
Number of Residues	15
Details	binding site for residue ADP D 501

Chain	Residue
D	HIS16
D	ILE17
D	ILE18
D	THR59
D	GLY60
D	VAL61
D	GLY62
D	LYS63
D	THR64
D	GLU65
D	LEU335
D	ILE343
D	ALA392
D	ARG393
D	HIS396

site_id	AE5
Number of Residues	3
Details	binding site for residue SO4 D 502

Chain	Residue
D	HIS46
D	LYS298
D	ASP300

site_id	AE6
Number of Residues	3
Details	binding site for residue SO4 D 503

Chain	Residue
D	THR369
D	SER371
J	LYS374

site_id	AE7
Number of Residues	13
Details	binding site for residue ADP E 501

Chain	Residue
E	HIS16
E	ILE17
E	ILE18
E	THR59
E	GLY60
E	VAL61
E	GLY62
E	LYS63
E	THR64
E	GLU65
E	LEU335
E	ALA392
E	ARG393

site_id	AE8
Number of Residues	4
Details	binding site for residue SO4 E 502

Chain	Residue
E	THR369
E	SER371
E	ASP421
E	ALA422

site_id	AE9
Number of Residues	14
Details	binding site for residue ADP F 501

Chain	Residue
F	HIS16
F	ILE17
F	ILE18
F	THR59
F	GLY60
F	VAL61
F	GLY62
F	LYS63
F	THR64
F	GLU65
F	ILE343
F	ALA392
F	ARG393
F	HIS396

site_id	AF1
Number of Residues	3
Details	binding site for residue SO4 F 502

Chain	Residue
F	THR369
F	SER371
F	ASP421

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	60
Details	BINDING:

Chain	Residue	Details
G	ILE18
H	ARG393
I	ILE18
I	GLY60
I	ASP256
I	GLU321
I	ARG393
J	ILE18
J	GLY60
J	ASP256
J	GLU321
G	GLY60
J	ARG393
K	ILE18
K	GLY60
K	ASP256
K	GLU321
K	ARG393
L	ILE18
L	GLY60
L	ASP256
L	GLU321
G	ASP256
L	ARG393
A	ILE18
A	GLY60
A	ASP256
A	GLU321
A	ARG393
B	ILE18
B	GLY60
B	ASP256
B	GLU321
G	GLU321
B	ARG393
C	ILE18
C	GLY60
C	ASP256
C	GLU321
C	ARG393
D	ILE18
D	GLY60
D	ASP256
D	GLU321
G	ARG393
D	ARG393
E	ILE18
E	GLY60
E	ASP256
E	GLU321
E	ARG393
F	ILE18
F	GLY60
F	ASP256
F	GLU321
H	ILE18
F	ARG393
H	GLY60
H	ASP256
H	GLU321

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)