6OKG

Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabF, and C16-crypto Acyl Carrier Protein, AcpP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004315	molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity
A	0005515	molecular_function	protein binding
A	0005829	cellular_component	cytosol
A	0006633	biological_process	fatty acid biosynthetic process
A	0009409	biological_process	response to cold
A	0016746	molecular_function	acyltransferase activity
A	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
A	0019367	biological_process	fatty acid elongation, saturated fatty acid
A	0042803	molecular_function	protein homodimerization activity
A	0044281	biological_process	small molecule metabolic process
A	1903966	biological_process	monounsaturated fatty acid biosynthetic process
B	0000035	molecular_function	acyl binding
B	0000036	molecular_function	acyl carrier activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006633	biological_process	fatty acid biosynthetic process
B	0008289	molecular_function	lipid binding
B	0008610	biological_process	lipid biosynthetic process
B	0009245	biological_process	lipid A biosynthetic process
B	0009410	biological_process	response to xenobiotic stimulus
B	0031177	molecular_function	phosphopantetheine binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue NA A 501

Chain	Residue
A	ASN301
A	ALA302
A	GLU349
A	ASN396

---

site_id	AC2
Number of Residues	26
Details	binding site for residue MU4 A 502

Chain	Residue
A	HIS145
A	SER156
A	ILE157
A	SER158
A	THR161
A	ALA162
A	CYS163
A	PHE202
A	ALA205
A	ARG206
A	ALA207
A	MET269
A	THR270
A	HIS303
A	THR305
A	HIS340
A	PHE398
A	GLY399
A	GLY402
A	THR403
B	SER36
A	PRO135
A	ASN140
A	MET141
A	ALA143
A	GLY144

---

Functional Information from PROSITE/UniProt

site_id	PS00012
Number of Residues	16
Details	PHOSPHOPANTETHEINE Phosphopantetheine attachment site. DLGADSLDTVELVMAL

Chain	Residue	Details
B	ASP31-LEU46

---

site_id	PS00606
Number of Residues	17
Details	KS3_1 Ketosynthase family 3 (KS3) active site signature. GPSisIAtACTSGvhNI

Chain	Residue	Details
A	GLY154-ILE170

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: O-(pantetheine 4'-phosphoryl)serine => ECO:0000255|PROSITE-ProRule:PRU00258, ECO:0000269|PubMed:4882207

Chain	Residue	Details
B	SER36

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: For beta-ketoacyl synthase activity => ECO:0000255|PROSITE-ProRule:PRU01348

Chain	Residue	Details
A	HIS303
A	HIS340

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:16710421, ECO:0000269|PubMed:19233644, ECO:0000269|PubMed:19581087, ECO:0007744|PDB:2GFX, ECO:0007744|PDB:3G0Y, ECO:0007744|PDB:3G11, ECO:0007744|PDB:3HNZ, ECO:0007744|PDB:3I8P

Chain	Residue	Details
A	THR270
A	THR307

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:16710421, ECO:0000269|PubMed:19233644, ECO:0007744|PDB:2GFX, ECO:0007744|PDB:3G0Y, ECO:0007744|PDB:3G11

Chain	Residue	Details
A	HIS303
A	HIS340

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 574

Chain	Residue	Details
A	CYS163	covalently attached, electrostatic stabiliser, nucleofuge, nucleophile, proton acceptor, proton donor
A	HIS303	activator, proton acceptor, proton donor
A	GLU314	electrostatic stabiliser
A	LYS335	electrostatic stabiliser
A	HIS340	electrostatic stabiliser, hydrogen bond donor
A	PHE400	electrostatic stabiliser

---