6OKF
Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabB, and C16-crypto Acyl Carrier Protein, AcpP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004315 | molecular_function | 3-oxoacyl-[acyl-carrier-protein] synthase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006633 | biological_process | fatty acid biosynthetic process |
| A | 0016746 | molecular_function | acyltransferase activity |
| A | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| A | 0044281 | biological_process | small molecule metabolic process |
| A | 1903966 | biological_process | monounsaturated fatty acid biosynthetic process |
| B | 0004315 | molecular_function | 3-oxoacyl-[acyl-carrier-protein] synthase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006633 | biological_process | fatty acid biosynthetic process |
| B | 0016746 | molecular_function | acyltransferase activity |
| B | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| B | 0044281 | biological_process | small molecule metabolic process |
| B | 1903966 | biological_process | monounsaturated fatty acid biosynthetic process |
| C | 0000035 | molecular_function | acyl binding |
| C | 0000036 | molecular_function | acyl carrier activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006633 | biological_process | fatty acid biosynthetic process |
| C | 0008289 | molecular_function | lipid binding |
| C | 0008610 | biological_process | lipid biosynthetic process |
| C | 0009245 | biological_process | lipid A biosynthetic process |
| C | 0009410 | biological_process | response to xenobiotic stimulus |
| C | 0031177 | molecular_function | phosphopantetheine binding |
| D | 0000035 | molecular_function | acyl binding |
| D | 0000036 | molecular_function | acyl carrier activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006633 | biological_process | fatty acid biosynthetic process |
| D | 0008289 | molecular_function | lipid binding |
| D | 0008610 | biological_process | lipid biosynthetic process |
| D | 0009245 | biological_process | lipid A biosynthetic process |
| D | 0009410 | biological_process | response to xenobiotic stimulus |
| D | 0031177 | molecular_function | phosphopantetheine binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | binding site for residue NA A 501 |
| Chain | Residue |
| A | ASN296 |
| A | SER297 |
| A | GLU342 |
| A | SER387 |
| A | ASN388 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | binding site for residue NA B 501 |
| Chain | Residue |
| B | ASN388 |
| B | ASN296 |
| B | SER297 |
| B | GLU342 |
| B | SER387 |
| site_id | AC3 |
| Number of Residues | 32 |
| Details | binding site for Di-peptide MRJ A 502 and SER D 36 |
| Chain | Residue |
| A | PHE35 |
| A | GLN37 |
| A | GLU38 |
| A | LEU39 |
| A | LYS40 |
| A | HIS47 |
| A | TRP49 |
| A | GLY106 |
| A | GLY107 |
| A | PRO110 |
| A | ALA162 |
| A | CYS163 |
| A | MET197 |
| A | GLU200 |
| A | PHE201 |
| A | VAL270 |
| A | THR300 |
| A | THR302 |
| A | GLY305 |
| A | PHE390 |
| A | GLY391 |
| A | PHE392 |
| A | HOH644 |
| B | GLN113 |
| B | ALA137 |
| B | MET138 |
| D | ASP35 |
| D | LEU37 |
| D | ASP38 |
| D | THR39 |
| D | VAL40 |
| D | HOH201 |
| site_id | AC4 |
| Number of Residues | 30 |
| Details | binding site for Di-peptide MRJ B 502 and SER C 36 |
| Chain | Residue |
| A | GLN113 |
| A | MET138 |
| B | THR34 |
| B | PHE35 |
| B | GLN37 |
| B | GLU38 |
| B | LEU39 |
| B | LYS40 |
| B | HIS47 |
| B | TRP49 |
| B | GLY106 |
| B | GLY107 |
| B | ALA162 |
| B | CYS163 |
| B | MET197 |
| B | GLU200 |
| B | PHE201 |
| B | MET204 |
| B | GLY205 |
| B | VAL270 |
| B | HIS298 |
| B | THR302 |
| B | PHE390 |
| B | GLY391 |
| B | PHE392 |
| C | ASP35 |
| C | LEU37 |
| C | ASP38 |
| C | THR39 |
| C | VAL40 |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | MOD_RES: O-(pantetheine 4'-phosphoryl)serine => ECO:0000255|PROSITE-ProRule:PRU00258, ECO:0000269|PubMed:4882207 |
| Chain | Residue | Details |
| C | SER36 | |
| D | SER36 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: For beta-ketoacyl synthase activity => ECO:0000255|PROSITE-ProRule:PRU01348 |
| Chain | Residue | Details |
| A | HIS298 | |
| A | HIS333 | |
| B | HIS298 | |
| B | HIS333 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 292 |
| Chain | Residue | Details |
| A | CYS163 | activator, covalently attached, electrostatic stabiliser, hydrogen bond donor, nucleofuge, nucleophile |
| A | HIS298 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| A | LYS328 | activator, hydrogen bond donor |
| A | HIS333 | electrostatic stabiliser, hydrogen bond donor, increase basicity |
| A | PHE390 | activator, hydrogen bond acceptor |
| A | PHE392 | electrostatic stabiliser, hydrogen bond donor |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 292 |
| Chain | Residue | Details |
| B | CYS163 | activator, covalently attached, electrostatic stabiliser, hydrogen bond donor, nucleofuge, nucleophile |
| B | HIS298 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| B | LYS328 | activator, hydrogen bond donor |
| B | HIS333 | electrostatic stabiliser, hydrogen bond donor, increase basicity |
| B | PHE390 | activator, hydrogen bond acceptor |
| B | PHE392 | electrostatic stabiliser, hydrogen bond donor |
