6NLH

Structure of human triose phosphate isomerase R189A

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004807	molecular_function	triose-phosphate isomerase activity
A	0005515	molecular_function	protein binding
A	0005615	cellular_component	extracellular space
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006006	biological_process	glucose metabolic process
A	0006094	biological_process	gluconeogenesis
A	0006096	biological_process	glycolytic process
A	0008929	molecular_function	methylglyoxal synthase activity
A	0016829	molecular_function	lyase activity
A	0016853	molecular_function	isomerase activity
A	0019242	biological_process	methylglyoxal biosynthetic process
A	0019563	biological_process	glycerol catabolic process
A	0019682	biological_process	glyceraldehyde-3-phosphate metabolic process
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0042803	molecular_function	protein homodimerization activity
A	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
A	0061621	biological_process	canonical glycolysis
A	0070062	cellular_component	extracellular exosome
B	0004807	molecular_function	triose-phosphate isomerase activity
B	0005515	molecular_function	protein binding
B	0005615	cellular_component	extracellular space
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006006	biological_process	glucose metabolic process
B	0006094	biological_process	gluconeogenesis
B	0006096	biological_process	glycolytic process
B	0008929	molecular_function	methylglyoxal synthase activity
B	0016829	molecular_function	lyase activity
B	0016853	molecular_function	isomerase activity
B	0019242	biological_process	methylglyoxal biosynthetic process
B	0019563	biological_process	glycerol catabolic process
B	0019682	biological_process	glyceraldehyde-3-phosphate metabolic process
B	0031625	molecular_function	ubiquitin protein ligase binding
B	0042803	molecular_function	protein homodimerization activity
B	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
B	0061621	biological_process	canonical glycolysis
B	0070062	cellular_component	extracellular exosome
C	0004807	molecular_function	triose-phosphate isomerase activity
C	0005515	molecular_function	protein binding
C	0005615	cellular_component	extracellular space
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006006	biological_process	glucose metabolic process
C	0006094	biological_process	gluconeogenesis
C	0006096	biological_process	glycolytic process
C	0008929	molecular_function	methylglyoxal synthase activity
C	0016829	molecular_function	lyase activity
C	0016853	molecular_function	isomerase activity
C	0019242	biological_process	methylglyoxal biosynthetic process
C	0019563	biological_process	glycerol catabolic process
C	0019682	biological_process	glyceraldehyde-3-phosphate metabolic process
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0042803	molecular_function	protein homodimerization activity
C	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
C	0061621	biological_process	canonical glycolysis
C	0070062	cellular_component	extracellular exosome
D	0004807	molecular_function	triose-phosphate isomerase activity
D	0005515	molecular_function	protein binding
D	0005615	cellular_component	extracellular space
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006006	biological_process	glucose metabolic process
D	0006094	biological_process	gluconeogenesis
D	0006096	biological_process	glycolytic process
D	0008929	molecular_function	methylglyoxal synthase activity
D	0016829	molecular_function	lyase activity
D	0016853	molecular_function	isomerase activity
D	0019242	biological_process	methylglyoxal biosynthetic process
D	0019563	biological_process	glycerol catabolic process
D	0019682	biological_process	glyceraldehyde-3-phosphate metabolic process
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0042803	molecular_function	protein homodimerization activity
D	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
D	0061621	biological_process	canonical glycolysis
D	0070062	cellular_component	extracellular exosome
E	0004807	molecular_function	triose-phosphate isomerase activity
E	0005515	molecular_function	protein binding
E	0005615	cellular_component	extracellular space
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006006	biological_process	glucose metabolic process
E	0006094	biological_process	gluconeogenesis
E	0006096	biological_process	glycolytic process
E	0008929	molecular_function	methylglyoxal synthase activity
E	0016829	molecular_function	lyase activity
E	0016853	molecular_function	isomerase activity
E	0019242	biological_process	methylglyoxal biosynthetic process
E	0019563	biological_process	glycerol catabolic process
E	0019682	biological_process	glyceraldehyde-3-phosphate metabolic process
E	0031625	molecular_function	ubiquitin protein ligase binding
E	0042803	molecular_function	protein homodimerization activity
E	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
E	0061621	biological_process	canonical glycolysis
E	0070062	cellular_component	extracellular exosome
F	0004807	molecular_function	triose-phosphate isomerase activity
F	0005515	molecular_function	protein binding
F	0005615	cellular_component	extracellular space
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006006	biological_process	glucose metabolic process
F	0006094	biological_process	gluconeogenesis
F	0006096	biological_process	glycolytic process
F	0008929	molecular_function	methylglyoxal synthase activity
F	0016829	molecular_function	lyase activity
F	0016853	molecular_function	isomerase activity
F	0019242	biological_process	methylglyoxal biosynthetic process
F	0019563	biological_process	glycerol catabolic process
F	0019682	biological_process	glyceraldehyde-3-phosphate metabolic process
F	0031625	molecular_function	ubiquitin protein ligase binding
F	0042803	molecular_function	protein homodimerization activity
F	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
F	0061621	biological_process	canonical glycolysis
F	0070062	cellular_component	extracellular exosome
G	0004807	molecular_function	triose-phosphate isomerase activity
G	0005515	molecular_function	protein binding
G	0005615	cellular_component	extracellular space
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006006	biological_process	glucose metabolic process
G	0006094	biological_process	gluconeogenesis
G	0006096	biological_process	glycolytic process
G	0008929	molecular_function	methylglyoxal synthase activity
G	0016829	molecular_function	lyase activity
G	0016853	molecular_function	isomerase activity
G	0019242	biological_process	methylglyoxal biosynthetic process
G	0019563	biological_process	glycerol catabolic process
G	0019682	biological_process	glyceraldehyde-3-phosphate metabolic process
G	0031625	molecular_function	ubiquitin protein ligase binding
G	0042803	molecular_function	protein homodimerization activity
G	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
G	0061621	biological_process	canonical glycolysis
G	0070062	cellular_component	extracellular exosome
H	0004807	molecular_function	triose-phosphate isomerase activity
H	0005515	molecular_function	protein binding
H	0005615	cellular_component	extracellular space
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006006	biological_process	glucose metabolic process
H	0006094	biological_process	gluconeogenesis
H	0006096	biological_process	glycolytic process
H	0008929	molecular_function	methylglyoxal synthase activity
H	0016829	molecular_function	lyase activity
H	0016853	molecular_function	isomerase activity
H	0019242	biological_process	methylglyoxal biosynthetic process
H	0019563	biological_process	glycerol catabolic process
H	0019682	biological_process	glyceraldehyde-3-phosphate metabolic process
H	0031625	molecular_function	ubiquitin protein ligase binding
H	0042803	molecular_function	protein homodimerization activity
H	0046166	biological_process	glyceraldehyde-3-phosphate biosynthetic process
H	0061621	biological_process	canonical glycolysis
H	0070062	cellular_component	extracellular exosome

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue NA A 301

Chain	Residue
A	ALA221
A	GLN223
A	VAL226
A	HOH502

---

site_id	AC2
Number of Residues	3
Details	binding site for residue NA A 302

Chain	Residue
A	ARG99
A	ASP106
A	GLN146

---

site_id	AC3
Number of Residues	4
Details	binding site for residue NA A 303

Chain	Residue
H	VAL69
H	THR70
A	SER194
A	ASN195

---

site_id	AC4
Number of Residues	3
Details	binding site for residue NA E 301

Chain	Residue
E	ALA221
E	GLN223
E	VAL226

---

site_id	AC5
Number of Residues	3
Details	binding site for residue BR E 302

Chain	Residue
E	ASN11
E	HIS95
E	LEU230

---

site_id	AC6
Number of Residues	6
Details	binding site for residue PO4 E 303

Chain	Residue
E	LYS13
E	SER211
E	GLY232
E	GLY233
E	HOH401
E	HOH417

---

site_id	AC7
Number of Residues	5
Details	binding site for residue NA B 301

Chain	Residue
B	ALA221
B	GLN223
B	VAL226
B	HOH429
B	HOH500

---

site_id	AC8
Number of Residues	2
Details	binding site for residue NA B 302

Chain	Residue
B	VAL39
B	LYS58

---

site_id	AC9
Number of Residues	3
Details	binding site for residue NA B 303

Chain	Residue
B	ARG98
B	GLU104
B	HOH456

---

site_id	AD1
Number of Residues	4
Details	binding site for residue NA B 304

Chain	Residue
B	SER194
B	ASN195
F	VAL69
F	THR70

---

site_id	AD2
Number of Residues	5
Details	binding site for residue NA C 301

Chain	Residue
B	ARG98
B	HOH405
C	GLU77
C	ARG98
C	HOH410

---

site_id	AD3
Number of Residues	4
Details	binding site for residue NA C 302

Chain	Residue
C	ALA221
C	GLN223
C	VAL226
C	LYS247

---

site_id	AD4
Number of Residues	3
Details	binding site for residue NA C 303

Chain	Residue
C	ARG99
C	ASP106
C	GLN146

---

site_id	AD5
Number of Residues	3
Details	binding site for residue BR C 304

Chain	Residue
C	ASN11
C	HIS95
C	HOH433

---

site_id	AD6
Number of Residues	6
Details	binding site for residue PO4 C 305

Chain	Residue
C	LYS13
C	GLY210
C	GLY232
C	GLY233
C	HOH433
C	HOH448

---

site_id	AD7
Number of Residues	4
Details	binding site for residue NA D 301

Chain	Residue
D	ALA221
D	GLN223
D	VAL226
D	HOH482

---

site_id	AD8
Number of Residues	3
Details	binding site for residue NA D 302

Chain	Residue
D	ARG99
D	ASP106
D	GLN146

---

site_id	AD9
Number of Residues	4
Details	binding site for residue NA D 303

Chain	Residue
C	VAL69
C	THR70
D	SER194
D	ASN195

---

site_id	AE1
Number of Residues	4
Details	binding site for residue NA F 301

Chain	Residue
F	ALA221
F	GLN223
F	VAL226
F	LYS247

---

site_id	AE2
Number of Residues	4
Details	binding site for residue NA F 302

Chain	Residue
F	GLU38
F	ALA60
F	TRP90
F	HOH465

---

site_id	AE3
Number of Residues	3
Details	binding site for residue BR F 303

Chain	Residue
F	ASN11
F	HIS95
F	HOH433

---

site_id	AE4
Number of Residues	4
Details	binding site for residue PO4 F 304

Chain	Residue
F	LYS13
F	GLY232
F	GLY233
F	HOH401

---

site_id	AE5
Number of Residues	3
Details	binding site for residue NA G 301

Chain	Residue
G	ARG99
G	ASP106
G	GLN146

---

site_id	AE6
Number of Residues	4
Details	binding site for residue NA G 302

Chain	Residue
G	ALA221
G	GLN223
G	VAL226
G	HOH475

---

site_id	AE7
Number of Residues	3
Details	binding site for residue NA H 301

Chain	Residue
H	ARG99
H	ASP106
H	GLN146

---

site_id	AE8
Number of Residues	4
Details	binding site for residue NA H 302

Chain	Residue
H	ALA221
H	GLN223
H	VAL226
H	LYS247

---

site_id	AE9
Number of Residues	2
Details	binding site for residue BR H 303

Chain	Residue
H	HIS95
H	ASN11

---

site_id	AF1
Number of Residues	5
Details	binding site for residue PO4 H 304

Chain	Residue
H	LYS13
H	SER211
H	GLY232
H	GLY233
H	HOH401

---

Functional Information from PROSITE/UniProt

site_id	PS00171
Number of Residues	11
Details	TIM_1 Triosephosphate isomerase active site. AYEPVWAIGTG

Chain	Residue	Details
A	ALA163-GLY173

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Active site: {"description":"Electrophile","evidences":[{"source":"PROSITE-ProRule","id":"PRU10127","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"8061610","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU10127","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"8061610","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU10127","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"8061610","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	16
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"16807684","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"17081983","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"17693683","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"17924679","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19367720","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"21406692","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	16
Details	Modified residue: {"description":"3'-nitrotyrosine","evidences":[{"source":"UniProtKB","id":"P17751","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	16
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P48500","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	8
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"P17751","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	16
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P17751","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P17751","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	4
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P17751","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	8
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	16
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)","evidences":[{"source":"PubMed","id":"25114211","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---