Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6N7I

Structure of bacteriophage T7 E343Q mutant gp4 helicase-primase in complex with ssDNA, dTTP, AC dinucleotide and CTP (gp4(5)-DNA)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0003677	molecular_function	DNA binding
A	0003678	molecular_function	DNA helicase activity
A	0003697	molecular_function	single-stranded DNA binding
A	0003824	molecular_function	catalytic activity
A	0003899	molecular_function	DNA-directed RNA polymerase activity
A	0004386	molecular_function	helicase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0006260	biological_process	DNA replication
A	0006269	biological_process	DNA replication, synthesis of primer
A	0008270	molecular_function	zinc ion binding
A	0016740	molecular_function	transferase activity
A	0016779	molecular_function	nucleotidyltransferase activity
A	0016787	molecular_function	hydrolase activity
A	0016853	molecular_function	isomerase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0039693	biological_process	viral DNA genome replication
A	0042802	molecular_function	identical protein binding
A	0043139	molecular_function	5'-3' DNA helicase activity
A	0046872	molecular_function	metal ion binding
B	0000166	molecular_function	nucleotide binding
B	0003677	molecular_function	DNA binding
B	0003678	molecular_function	DNA helicase activity
B	0003697	molecular_function	single-stranded DNA binding
B	0003824	molecular_function	catalytic activity
B	0003899	molecular_function	DNA-directed RNA polymerase activity
B	0004386	molecular_function	helicase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0006260	biological_process	DNA replication
B	0006269	biological_process	DNA replication, synthesis of primer
B	0008270	molecular_function	zinc ion binding
B	0016740	molecular_function	transferase activity
B	0016779	molecular_function	nucleotidyltransferase activity
B	0016787	molecular_function	hydrolase activity
B	0016853	molecular_function	isomerase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0039693	biological_process	viral DNA genome replication
B	0042802	molecular_function	identical protein binding
B	0043139	molecular_function	5'-3' DNA helicase activity
B	0046872	molecular_function	metal ion binding
C	0000166	molecular_function	nucleotide binding
C	0003677	molecular_function	DNA binding
C	0003678	molecular_function	DNA helicase activity
C	0003697	molecular_function	single-stranded DNA binding
C	0003824	molecular_function	catalytic activity
C	0003899	molecular_function	DNA-directed RNA polymerase activity
C	0004386	molecular_function	helicase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0006260	biological_process	DNA replication
C	0006269	biological_process	DNA replication, synthesis of primer
C	0008270	molecular_function	zinc ion binding
C	0016740	molecular_function	transferase activity
C	0016779	molecular_function	nucleotidyltransferase activity
C	0016787	molecular_function	hydrolase activity
C	0016853	molecular_function	isomerase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0039693	biological_process	viral DNA genome replication
C	0042802	molecular_function	identical protein binding
C	0043139	molecular_function	5'-3' DNA helicase activity
C	0046872	molecular_function	metal ion binding
D	0000166	molecular_function	nucleotide binding
D	0003677	molecular_function	DNA binding
D	0003678	molecular_function	DNA helicase activity
D	0003697	molecular_function	single-stranded DNA binding
D	0003824	molecular_function	catalytic activity
D	0003899	molecular_function	DNA-directed RNA polymerase activity
D	0004386	molecular_function	helicase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0006260	biological_process	DNA replication
D	0006269	biological_process	DNA replication, synthesis of primer
D	0008270	molecular_function	zinc ion binding
D	0016740	molecular_function	transferase activity
D	0016779	molecular_function	nucleotidyltransferase activity
D	0016787	molecular_function	hydrolase activity
D	0016853	molecular_function	isomerase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0039693	biological_process	viral DNA genome replication
D	0042802	molecular_function	identical protein binding
D	0043139	molecular_function	5'-3' DNA helicase activity
D	0046872	molecular_function	metal ion binding
E	0000166	molecular_function	nucleotide binding
E	0003677	molecular_function	DNA binding
E	0003678	molecular_function	DNA helicase activity
E	0003697	molecular_function	single-stranded DNA binding
E	0003824	molecular_function	catalytic activity
E	0003899	molecular_function	DNA-directed RNA polymerase activity
E	0004386	molecular_function	helicase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0006260	biological_process	DNA replication
E	0006269	biological_process	DNA replication, synthesis of primer
E	0008270	molecular_function	zinc ion binding
E	0016740	molecular_function	transferase activity
E	0016779	molecular_function	nucleotidyltransferase activity
E	0016787	molecular_function	hydrolase activity
E	0016853	molecular_function	isomerase activity
E	0016887	molecular_function	ATP hydrolysis activity
E	0039693	biological_process	viral DNA genome replication
E	0042802	molecular_function	identical protein binding
E	0043139	molecular_function	5'-3' DNA helicase activity
E	0046872	molecular_function	metal ion binding
F	0000166	molecular_function	nucleotide binding
F	0003677	molecular_function	DNA binding
F	0003678	molecular_function	DNA helicase activity
F	0003697	molecular_function	single-stranded DNA binding
F	0003824	molecular_function	catalytic activity
F	0003899	molecular_function	DNA-directed RNA polymerase activity
F	0004386	molecular_function	helicase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0006260	biological_process	DNA replication
F	0006269	biological_process	DNA replication, synthesis of primer
F	0008270	molecular_function	zinc ion binding
F	0016740	molecular_function	transferase activity
F	0016779	molecular_function	nucleotidyltransferase activity
F	0016787	molecular_function	hydrolase activity
F	0016853	molecular_function	isomerase activity
F	0016887	molecular_function	ATP hydrolysis activity
F	0039693	biological_process	viral DNA genome replication
F	0042802	molecular_function	identical protein binding
F	0043139	molecular_function	5'-3' DNA helicase activity
F	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	17
Details	binding site for residue TTP B 700

Chain	Residue
A	LYS520
B	GLN343
B	HIS465
B	ARG504
B	ASN512
B	VAL514
B	TYR535
B	LYS537
B	MG701
A	ARG522
A	THR524
B	GLY315
B	MET316
B	GLY317
B	LYS318
B	SER319
B	THR320

site_id	AC2
Number of Residues	3
Details	binding site for residue MG B 701

Chain	Residue
B	SER319
B	GLN343
B	TTP700

site_id	AC3
Number of Residues	16
Details	binding site for residue TTP C 700

Chain	Residue
B	LYS520
B	ARG522
B	PHE523
B	GLY525
C	GLY315
C	MET316
C	GLY317
C	LYS318
C	SER319
C	THR320
C	GLN343
C	HIS465
C	ARG504
C	ASN512
C	TYR535
C	MG701

site_id	AC4
Number of Residues	3
Details	binding site for residue MG C 701

Chain	Residue
C	SER319
C	GLN343
C	TTP700

site_id	AC5
Number of Residues	17
Details	binding site for residue TTP D 700

Chain	Residue
C	ARG522
C	PHE523
C	THR524
C	GLY525
D	GLY313
D	GLY315
D	MET316
D	GLY317
D	LYS318
D	SER319
D	THR320
D	GLN343
D	HIS465
D	ARG504
D	TYR535
D	LYS537
D	MG701

site_id	AC6
Number of Residues	4
Details	binding site for residue MG D 701

Chain	Residue
D	SER319
D	GLN343
D	ASP424
D	TTP700

site_id	AC7
Number of Residues	19
Details	binding site for residue TTP E 700

Chain	Residue
D	ARG522
D	THR524
D	HOH801
E	GLY313
E	SER314
E	GLY315
E	MET316
E	GLY317
E	LYS318
E	SER319
E	THR320
E	GLN343
E	HIS465
E	ARG504
E	PRO511
E	ASN512
E	VAL514
E	TYR535
E	MG701

site_id	AC8
Number of Residues	3
Details	binding site for residue MG E 701

Chain	Residue
E	SER319
E	GLN343
E	TTP700

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	35
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_04154","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	5
Details	Site: {"description":"dTTP/dATP binding","evidences":[{"source":"HAMAP-Rule","id":"MF_04154","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10892646","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	5
Details	Site: {"description":"dTTP/dATP binding","evidences":[{"source":"HAMAP-Rule","id":"MF_04154","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10535735","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	15
Details	Site: {"description":"dTTP/dATP binding","evidences":[{"source":"HAMAP-Rule","id":"MF_04154","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10535735","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"10892646","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)