6N4Q

CryoEM structure of Nav1.7 VSD2 (actived state) in complex with the gating modifier toxin ProTx2

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005216	molecular_function	monoatomic ion channel activity
A	0005261	molecular_function	monoatomic cation channel activity
A	0005886	cellular_component	plasma membrane
A	0006811	biological_process	monoatomic ion transport
A	0016020	cellular_component	membrane
A	0055085	biological_process	transmembrane transport
B	0005216	molecular_function	monoatomic ion channel activity
B	0005261	molecular_function	monoatomic cation channel activity
B	0005886	cellular_component	plasma membrane
B	0006811	biological_process	monoatomic ion transport
B	0016020	cellular_component	membrane
B	0055085	biological_process	transmembrane transport
C	0005216	molecular_function	monoatomic ion channel activity
C	0005261	molecular_function	monoatomic cation channel activity
C	0005886	cellular_component	plasma membrane
C	0006811	biological_process	monoatomic ion transport
C	0016020	cellular_component	membrane
C	0055085	biological_process	transmembrane transport
D	0005216	molecular_function	monoatomic ion channel activity
D	0005261	molecular_function	monoatomic cation channel activity
D	0005886	cellular_component	plasma membrane
D	0006811	biological_process	monoatomic ion transport
D	0016020	cellular_component	membrane
D	0055085	biological_process	transmembrane transport
E	0005246	molecular_function	calcium channel regulator activity
E	0005576	cellular_component	extracellular region
E	0008289	molecular_function	lipid binding
E	0017080	molecular_function	sodium channel regulator activity
E	0035821	biological_process	modulation of process of another organism
E	0090729	molecular_function	toxin activity
E	0099106	molecular_function	ion channel regulator activity
F	0005246	molecular_function	calcium channel regulator activity
F	0005576	cellular_component	extracellular region
F	0008289	molecular_function	lipid binding
F	0017080	molecular_function	sodium channel regulator activity
F	0035821	biological_process	modulation of process of another organism
F	0090729	molecular_function	toxin activity
F	0099106	molecular_function	ion channel regulator activity
G	0005246	molecular_function	calcium channel regulator activity
G	0005576	cellular_component	extracellular region
G	0008289	molecular_function	lipid binding
G	0017080	molecular_function	sodium channel regulator activity
G	0035821	biological_process	modulation of process of another organism
G	0090729	molecular_function	toxin activity
G	0099106	molecular_function	ion channel regulator activity
H	0005246	molecular_function	calcium channel regulator activity
H	0005576	cellular_component	extracellular region
H	0008289	molecular_function	lipid binding
H	0017080	molecular_function	sodium channel regulator activity
H	0035821	biological_process	modulation of process of another organism
H	0090729	molecular_function	toxin activity
H	0099106	molecular_function	ion channel regulator activity

Functional Information from PROSITE/UniProt

site_id	PS00290
Number of Residues	7
Details	IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. YTCEATH

Chain	Residue	Details
I	TYR193-HIS199

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	SITE: Part of an aromatic-rich surface that anchors the toxin toward the membrane core relative to lipid headgroups bound along the pore module of Nav1.7/SCN9A => ECO:0000305|PubMed:30661758

Chain	Residue	Details
E	TRP5
H	TRP5
H	TRP7
H	TRP30
E	TRP7
E	TRP30
F	TRP5
F	TRP7
F	TRP30
G	TRP5
G	TRP7
G	TRP30

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site_id	SWS_FT_FI2
Number of Residues	4
Details	SITE: Electrostatic gating-modifier of Nav1.7/SCN9A that antagonizes outward gating-charge movement through direct electrostatic repulsion; may also indirectly antagonize S4 gating-charge movement by neutralizing acidic side chains within the extracellular vestibule of VSD2 => ECO:0000305|PubMed:30661758

Chain	Residue	Details
E	ARG22
F	ARG22
G	ARG22
H	ARG22
C	GLU810
C	ASP815
D	GLU810
D	ASP815

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site_id	SWS_FT_FI3
Number of Residues	4
Details	SITE: Part of an aromatic-rich surface that anchors the toxin toward the membrane core relative to lipid headgroups bound along the pore module of Nav1.7/SCN9A; it also stabilizes the toxin for productive receptor site engagement => ECO:0000305|PubMed:30661758

Chain	Residue	Details
E	TRP24
F	TRP24
G	TRP24
H	TRP24

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site_id	SWS_FT_FI4
Number of Residues	4
Details	SITE: Antagonizes outward gating-charge movement of Nav1.7/SCN9A through direct electrostatic repulsion; may also indirectly antagonize S4 gating-charge movement by neutralizing acidic side chains within the extracellular vestibule of VSD2 => ECO:0000305|PubMed:30661758

Chain	Residue	Details
E	LYS26
F	LYS26
G	LYS26
H	LYS26

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