6MT8
E. coli DHFR complex modeled with two ligand states
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005542 | molecular_function | folic acid binding |
| A | 0005829 | cellular_component | cytosol |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0009257 | biological_process | 10-formyltetrahydrofolate biosynthetic process |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0031427 | biological_process | response to methotrexate |
| A | 0046452 | biological_process | dihydrofolate metabolic process |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| A | 0046655 | biological_process | folic acid metabolic process |
| A | 0046656 | biological_process | folic acid biosynthetic process |
| A | 0046677 | biological_process | response to antibiotic |
| A | 0050661 | molecular_function | NADP binding |
| A | 0051870 | molecular_function | methotrexate binding |
| A | 0051871 | molecular_function | dihydrofolic acid binding |
| A | 0070401 | molecular_function | NADP+ binding |
| A | 0070402 | molecular_function | NADPH binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | binding site for residue CL A 201 |
| Chain | Residue |
| A | GLY43 |
| A | HIS45 |
| A | THR46 |
| A | GLY96 |
| A | HOH432 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | binding site for residue CL A 202 |
| Chain | Residue |
| A | HOH480 |
| A | LYS32 |
| A | ASP144 |
| A | ALA145 |
| A | HOH417 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | binding site for residue CL A 203 |
| Chain | Residue |
| A | TRP30 |
| A | ARG33 |
| A | HOH368 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue MG A 204 |
| Chain | Residue |
| A | GLU101 |
| A | GLU139 |
| A | HOH336 |
| A | HOH352 |
| A | HOH388 |
| A | HOH420 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | binding site for residue MG A 205 |
| Chain | Residue |
| A | GLU101 |
| A | SER138 |
| A | HOH370 |
| A | HOH445 |
| A | HOH457 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | binding site for residue MG A 206 |
| Chain | Residue |
| A | HIS160 |
| A | HOH326 |
| A | HOH380 |
| A | HOH415 |
| A | HOH443 |
| A | HOH486 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | binding site for residue MG A 207 |
| Chain | Residue |
| A | ASP70 |
| A | HOH313 |
| A | HOH341 |
| A | HOH385 |
| A | HOH392 |
| A | HOH465 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 23 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGmenaMPWnlpa.DlawFkrnT |
| Chain | Residue | Details |
| A | VAL13-THR35 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 5 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:9012674 |
| Chain | Residue | Details |
| A | ILE5 | |
| A | ASP27 | |
| A | ARG52 | |
| A | ARG57 | |
| A | THR113 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:19374017 |
| Chain | Residue | Details |
| A | ALA7 | |
| A | VAL13 | |
| A | HIS45 | |
| A | SER63 | |
| A | LYS76 | |
| A | GLY95 |
