6LH9
Quadruple mutant (N51I+C59R+S108N+I164L) plasmodium falciparum dihydrofolate reductase-thymidylate synthase (PfDHFR-TS) complexed with compound 46 and NADPH
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0004799 | molecular_function | thymidylate synthase activity |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005829 | cellular_component | cytosol |
| A | 0006231 | biological_process | dTMP biosynthetic process |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009165 | biological_process | nucleotide biosynthetic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| A | 0032259 | biological_process | methylation |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0004146 | molecular_function | dihydrofolate reductase activity |
| B | 0004799 | molecular_function | thymidylate synthase activity |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005829 | cellular_component | cytosol |
| B | 0006231 | biological_process | dTMP biosynthetic process |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0009165 | biological_process | nucleotide biosynthetic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| B | 0032259 | biological_process | methylation |
| B | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | binding site for residue PO4 A 801 |
| Chain | Residue |
| A | ARG345 |
| A | ARG510 |
| A | SER511 |
| B | ARG470 |
| B | ARG471 |
| site_id | AC2 |
| Number of Residues | 11 |
| Details | binding site for residue EA0 A 802 |
| Chain | Residue |
| A | MET55 |
| A | PHE58 |
| A | ASN108 |
| A | PHE116 |
| A | LEU164 |
| A | TYR170 |
| A | NDP803 |
| A | ILE14 |
| A | CYS15 |
| A | ALA16 |
| A | ASP54 |
| site_id | AC3 |
| Number of Residues | 24 |
| Details | binding site for residue NDP A 803 |
| Chain | Residue |
| A | CYS15 |
| A | ALA16 |
| A | LEU40 |
| A | GLY44 |
| A | VAL45 |
| A | LEU46 |
| A | GLY105 |
| A | ARG106 |
| A | THR107 |
| A | ASN108 |
| A | LEU127 |
| A | SER128 |
| A | ARG129 |
| A | THR130 |
| A | ASN144 |
| A | VAL146 |
| A | LEU164 |
| A | GLY166 |
| A | SER167 |
| A | VAL168 |
| A | VAL169 |
| A | TYR170 |
| A | GLU172 |
| A | EA0802 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue PO4 A 804 |
| Chain | Residue |
| A | ARG470 |
| A | ARG471 |
| B | ARG345 |
| B | ARG510 |
| B | SER511 |
| site_id | AC5 |
| Number of Residues | 11 |
| Details | binding site for residue EA0 B 901 |
| Chain | Residue |
| B | ILE14 |
| B | CYS15 |
| B | ALA16 |
| B | ASP54 |
| B | MET55 |
| B | PHE58 |
| B | ASN108 |
| B | LEU164 |
| B | TYR170 |
| B | THR185 |
| B | NDP902 |
| site_id | AC6 |
| Number of Residues | 25 |
| Details | binding site for residue NDP B 902 |
| Chain | Residue |
| B | CYS15 |
| B | ALA16 |
| B | LEU40 |
| B | GLY41 |
| B | ASN42 |
| B | GLY44 |
| B | VAL45 |
| B | LEU46 |
| B | GLY105 |
| B | ARG106 |
| B | THR107 |
| B | ASN108 |
| B | LEU127 |
| B | SER128 |
| B | ARG129 |
| B | THR130 |
| B | ASN144 |
| B | LEU164 |
| B | GLY166 |
| B | SER167 |
| B | VAL168 |
| B | VAL169 |
| B | TYR170 |
| B | GLU172 |
| B | EA0901 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 24 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. GLGnkgvLPWkcislDmkyFravT |
| Chain | Residue | Details |
| A | GLY39-THR62 |
| site_id | PS00091 |
| Number of Residues | 29 |
| Details | THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriLlcaWNvkdldqma.....LpPCHilcQFyV |
| Chain | Residue | Details |
| A | ARG470-VAL498 |
