Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6KOE

X-ray Structure of the proton-pumping cytochrome aa3-600 menaquinol oxidase from Bacillus subtilis

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004129	molecular_function	cytochrome-c oxidase activity
A	0005507	molecular_function	copper ion binding
A	0005886	cellular_component	plasma membrane
A	0006119	biological_process	oxidative phosphorylation
A	0009060	biological_process	aerobic respiration
A	0015990	biological_process	electron transport coupled proton transport
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0016682	molecular_function	oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor
A	0020037	molecular_function	heme binding
A	0022904	biological_process	respiratory electron transport chain
A	0042773	biological_process	ATP synthesis coupled electron transport
A	0046872	molecular_function	metal ion binding
A	0070469	cellular_component	respirasome
A	1902600	biological_process	proton transmembrane transport
B	0004129	molecular_function	cytochrome-c oxidase activity
B	0005507	molecular_function	copper ion binding
B	0005886	cellular_component	plasma membrane
B	0009486	molecular_function	cytochrome bo3 ubiquinol oxidase activity
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0016682	molecular_function	oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor
B	0022900	biological_process	electron transport chain
B	0042773	biological_process	ATP synthesis coupled electron transport
B	0045121	cellular_component	membrane raft
B	0055085	biological_process	transmembrane transport
B	0070469	cellular_component	respirasome
B	1902600	biological_process	proton transmembrane transport
C	0004129	molecular_function	cytochrome-c oxidase activity
C	0005886	cellular_component	plasma membrane
C	0009055	molecular_function	electron transfer activity
C	0009319	cellular_component	cytochrome o ubiquinol oxidase complex
C	0009486	molecular_function	cytochrome bo3 ubiquinol oxidase activity
C	0015990	biological_process	electron transport coupled proton transport
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0016682	molecular_function	oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor
C	0019646	biological_process	aerobic electron transport chain
C	0022904	biological_process	respiratory electron transport chain
C	0042773	biological_process	ATP synthesis coupled electron transport
D	0016020	cellular_component	membrane
E	0004129	molecular_function	cytochrome-c oxidase activity
E	0005507	molecular_function	copper ion binding
E	0005886	cellular_component	plasma membrane
E	0006119	biological_process	oxidative phosphorylation
E	0009060	biological_process	aerobic respiration
E	0015990	biological_process	electron transport coupled proton transport
E	0016020	cellular_component	membrane
E	0016491	molecular_function	oxidoreductase activity
E	0016682	molecular_function	oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor
E	0020037	molecular_function	heme binding
E	0022904	biological_process	respiratory electron transport chain
E	0042773	biological_process	ATP synthesis coupled electron transport
E	0046872	molecular_function	metal ion binding
E	0070469	cellular_component	respirasome
E	1902600	biological_process	proton transmembrane transport
F	0004129	molecular_function	cytochrome-c oxidase activity
F	0005507	molecular_function	copper ion binding
F	0005886	cellular_component	plasma membrane
F	0009486	molecular_function	cytochrome bo3 ubiquinol oxidase activity
F	0016020	cellular_component	membrane
F	0016491	molecular_function	oxidoreductase activity
F	0016682	molecular_function	oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor
F	0022900	biological_process	electron transport chain
F	0042773	biological_process	ATP synthesis coupled electron transport
F	0045121	cellular_component	membrane raft
F	0055085	biological_process	transmembrane transport
F	0070469	cellular_component	respirasome
F	1902600	biological_process	proton transmembrane transport
G	0004129	molecular_function	cytochrome-c oxidase activity
G	0005886	cellular_component	plasma membrane
G	0009055	molecular_function	electron transfer activity
G	0009319	cellular_component	cytochrome o ubiquinol oxidase complex
G	0009486	molecular_function	cytochrome bo3 ubiquinol oxidase activity
G	0015990	biological_process	electron transport coupled proton transport
G	0016020	cellular_component	membrane
G	0016491	molecular_function	oxidoreductase activity
G	0016682	molecular_function	oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor
G	0019646	biological_process	aerobic electron transport chain
G	0022904	biological_process	respiratory electron transport chain
G	0042773	biological_process	ATP synthesis coupled electron transport
H	0016020	cellular_component	membrane

Functional Information from PDB Data

site_id	AC1
Number of Residues	27
Details	binding site for residue HEA A 1001

Chain	Residue
A	PHE69
A	GLY103
A	MET106
A	ILE107
A	GLY165
A	TRP166
A	TYR410
A	PHE416
A	HIS417
A	LEU420
A	ILE421
A	GLY72
A	VAL425
A	GLN467
A	ARG477
A	ARG478
A	ALA499
A	MET502
A	GLY503
A	PHE506
A	VAL73
A	LEU76
A	ARG79
A	TYR95
A	PHE99
A	THR100
A	HIS102

site_id	AC2
Number of Residues	22
Details	binding site for residue HEA A 1002

Chain	Residue
A	TRP166
A	TRP276
A	VAL283
A	TYR284
A	ILE287
A	HIS329
A	HIS330
A	THR348
A	SER352
A	GLY391
A	GLY394
A	LEU397
A	ALA398
A	ASP403
A	HIS407
A	LEU412
A	HIS415
A	PHE416
A	VAL419
A	LEU420
B	PHE36
B	ILE78

site_id	AC3
Number of Residues	3
Details	binding site for residue CU A 1003

Chain	Residue
A	HIS280
A	HIS329
A	HIS330

site_id	AC4
Number of Residues	9
Details	binding site for residue HQO A 1004

Chain	Residue
A	ARG70
A	VAL73
A	ASP74
A	HIS94
A	GLU97
A	ILE98
A	THR101
A	PHE156
A	SER161

site_id	AC5
Number of Residues	24
Details	binding site for residue HEA E 1001

Chain	Residue
E	PHE69
E	GLY72
E	MET78
E	ARG79
E	TYR95
E	PHE99
E	THR100
E	HIS102
E	GLY103
E	MET106
E	GLY165
E	TRP166
E	TYR410
E	PHE416
E	HIS417
E	LEU420
E	ILE421
E	GLN467
E	ARG477
E	ARG478
E	ALA499
E	MET502
E	GLY503
E	PHE506

site_id	AC6
Number of Residues	23
Details	binding site for residue HEA E 1002

Chain	Residue
E	ALA398
E	ASP403
E	HIS407
E	LEU412
E	HIS415
E	PHE416
E	VAL419
E	LEU420
F	PHE36
E	TRP166
E	TRP276
E	VAL283
E	TYR284
E	ILE287
E	HIS329
E	HIS330
E	THR348
E	SER352
E	GLY356
E	ILE359
E	GLY391
E	GLY394
E	LEU397

site_id	AC7
Number of Residues	3
Details	binding site for residue CU E 1003

Chain	Residue
E	HIS280
E	HIS329
E	HIS330

site_id	AC8
Number of Residues	9
Details	binding site for residue HQO E 1004

Chain	Residue
E	LEU17
E	ARG70
E	VAL73
E	ASP74
E	HIS94
E	GLU97
E	ILE98
E	PHE156
E	SER161

Functional Information from PROSITE/UniProt

site_id	PS00077
Number of Residues	55
Details	COX1_CUB Heme-copper oxidase catalytic subunit, copper B binding region signature. WIWGHPeVyivilpafgifseiissfarkqlfgykamvgsiiaisvlsflvwt..HH

Chain	Residue	Details
A	TRP276-HIS330

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	40
Details	TRANSMEM: Helical => ECO:0000255

Chain	Residue	Details
D	THR78-ALA98
G	PHE118-PHE138
G	LEU140-ILE160
G	PHE184-LEU204
E	ASN336-PHE343
E	ALA398-GLN405
E	GLN473-THR490
E	PHE605-MET610
H	THR78-ALA98
C	GLY95-ILE115
C	PHE118-PHE138
C	LEU140-ILE160
C	PHE184-LEU204
G	PHE27-VAL47
G	LEU66-HIS86
G	GLY95-ILE115

site_id	SWS_FT_FI2
Number of Residues	2
Details	LIPID: S-diacylglycerol cysteine => ECO:0000255\|PROSITE-ProRule:PRU00303

Chain	Residue	Details
B	CYS1
A	LEU378-LEU397
A	TYR406-VAL425
A	PHE453-LEU472
A	LEU491-CYS510
A	ASN585-VAL604
A	GLY611-ASN631
E	ILE16-LEU34
E	GLY57-GLY75
E	ILE98-GLY117
E	LEU140-VAL157
F	CYS1
E	GLN191-LEU209
E	THR228-LEU246
E	ASN273-GLY292
E	ILE316-GLY335
E	PHE344-TRP362
E	LEU378-LEU397
E	TYR406-VAL425
E	PHE453-LEU472
E	LEU491-CYS510
E	ASN585-VAL604
A	ILE98-GLY117
E	GLY611-ASN631
A	LEU140-VAL157
A	GLN191-LEU209
A	THR228-LEU246
A	ASN273-GLY292
A	ILE316-GLY335
A	PHE344-TRP362

site_id	SWS_FT_FI3
Number of Residues	422
Details	TOPO_DOM: Cytoplasmic => ECO:0000255

Chain	Residue	Details
A	THR35-LEU56
E	LEU118-ASN139
E	LYS210-THR227
E	ILE293-SER315
E	LEU363-MET377
E	PHE426-PHE452
E	TYR511-SER584
E	GLY632-GLU649
A	LEU118-ASN139
A	LYS210-THR227
A	ILE293-SER315
A	LEU363-MET377
A	PHE426-PHE452
A	TYR511-SER584
A	GLY632-GLU649
E	THR35-LEU56

site_id	SWS_FT_FI4
Number of Residues	6
Details	BINDING: axial binding residue => ECO:0000250

Chain	Residue	Details
A	HIS102
A	HIS415
A	HIS417
E	HIS102
E	HIS415
E	HIS417

site_id	SWS_FT_FI5
Number of Residues	8
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	HIS280
A	TYR284
A	HIS329
A	HIS330
E	HIS280
E	TYR284
E	HIS329
E	HIS330

site_id	SWS_FT_FI6
Number of Residues	4
Details	CROSSLNK: 1'-histidyl-3'-tyrosine (His-Tyr) => ECO:0000250

Chain	Residue	Details
A	HIS280
A	TYR284
E	HIS280
E	TYR284

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)