6JD1
Cryo-EM Structure of Sulfolobus solfataricus ketol-acid reductoisomerase (Sso-KARI) in complex with Mg2+, NADH, and CPD at pH7.5
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
A | 0005829 | cellular_component | cytosol |
A | 0008652 | biological_process | amino acid biosynthetic process |
A | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
A | 0009097 | biological_process | isoleucine biosynthetic process |
A | 0009099 | biological_process | valine biosynthetic process |
A | 0016491 | molecular_function | oxidoreductase activity |
B | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
B | 0005829 | cellular_component | cytosol |
B | 0008652 | biological_process | amino acid biosynthetic process |
B | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
B | 0009097 | biological_process | isoleucine biosynthetic process |
B | 0009099 | biological_process | valine biosynthetic process |
B | 0016491 | molecular_function | oxidoreductase activity |
C | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
C | 0005829 | cellular_component | cytosol |
C | 0008652 | biological_process | amino acid biosynthetic process |
C | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
C | 0009097 | biological_process | isoleucine biosynthetic process |
C | 0009099 | biological_process | valine biosynthetic process |
C | 0016491 | molecular_function | oxidoreductase activity |
D | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
D | 0005829 | cellular_component | cytosol |
D | 0008652 | biological_process | amino acid biosynthetic process |
D | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
D | 0009097 | biological_process | isoleucine biosynthetic process |
D | 0009099 | biological_process | valine biosynthetic process |
D | 0016491 | molecular_function | oxidoreductase activity |
E | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
E | 0005829 | cellular_component | cytosol |
E | 0008652 | biological_process | amino acid biosynthetic process |
E | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
E | 0009097 | biological_process | isoleucine biosynthetic process |
E | 0009099 | biological_process | valine biosynthetic process |
E | 0016491 | molecular_function | oxidoreductase activity |
F | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
F | 0005829 | cellular_component | cytosol |
F | 0008652 | biological_process | amino acid biosynthetic process |
F | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
F | 0009097 | biological_process | isoleucine biosynthetic process |
F | 0009099 | biological_process | valine biosynthetic process |
F | 0016491 | molecular_function | oxidoreductase activity |
G | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
G | 0005829 | cellular_component | cytosol |
G | 0008652 | biological_process | amino acid biosynthetic process |
G | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
G | 0009097 | biological_process | isoleucine biosynthetic process |
G | 0009099 | biological_process | valine biosynthetic process |
G | 0016491 | molecular_function | oxidoreductase activity |
H | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
H | 0005829 | cellular_component | cytosol |
H | 0008652 | biological_process | amino acid biosynthetic process |
H | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
H | 0009097 | biological_process | isoleucine biosynthetic process |
H | 0009099 | biological_process | valine biosynthetic process |
H | 0016491 | molecular_function | oxidoreductase activity |
I | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
I | 0005829 | cellular_component | cytosol |
I | 0008652 | biological_process | amino acid biosynthetic process |
I | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
I | 0009097 | biological_process | isoleucine biosynthetic process |
I | 0009099 | biological_process | valine biosynthetic process |
I | 0016491 | molecular_function | oxidoreductase activity |
J | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
J | 0005829 | cellular_component | cytosol |
J | 0008652 | biological_process | amino acid biosynthetic process |
J | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
J | 0009097 | biological_process | isoleucine biosynthetic process |
J | 0009099 | biological_process | valine biosynthetic process |
J | 0016491 | molecular_function | oxidoreductase activity |
K | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
K | 0005829 | cellular_component | cytosol |
K | 0008652 | biological_process | amino acid biosynthetic process |
K | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
K | 0009097 | biological_process | isoleucine biosynthetic process |
K | 0009099 | biological_process | valine biosynthetic process |
K | 0016491 | molecular_function | oxidoreductase activity |
L | 0004455 | molecular_function | ketol-acid reductoisomerase activity |
L | 0005829 | cellular_component | cytosol |
L | 0008652 | biological_process | amino acid biosynthetic process |
L | 0009082 | biological_process | branched-chain amino acid biosynthetic process |
L | 0009097 | biological_process | isoleucine biosynthetic process |
L | 0009099 | biological_process | valine biosynthetic process |
L | 0016491 | molecular_function | oxidoreductase activity |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 4 |
Details | binding site for residue MG A 401 |
Chain | Residue |
A | ASP191 |
A | LEU192 |
A | GLU195 |
A | 9TY405 |
site_id | AC2 |
Number of Residues | 5 |
Details | binding site for residue MG A 402 |
Chain | Residue |
A | ARG130 |
A | ASP191 |
A | 9TY405 |
B | GLU227 |
B | GLU233 |
site_id | AC3 |
Number of Residues | 1 |
Details | binding site for residue MG A 403 |
Chain | Residue |
A | NAI404 |
site_id | AC4 |
Number of Residues | 14 |
Details | binding site for residue NAI A 404 |
Chain | Residue |
A | GLY24 |
A | ASN25 |
A | GLN26 |
A | ASN45 |
A | VAL46 |
A | TYR50 |
A | ILE78 |
A | PRO79 |
A | ASP80 |
A | SER107 |
A | MET131 |
A | VAL132 |
A | MG403 |
A | 9TY405 |
site_id | AC5 |
Number of Residues | 7 |
Details | binding site for residue 9TY A 405 |
Chain | Residue |
A | ASP191 |
A | GLU195 |
A | MG401 |
A | MG402 |
A | NAI404 |
B | GLU233 |
B | SER257 |
site_id | AC6 |
Number of Residues | 4 |
Details | binding site for residue MG B 401 |
Chain | Residue |
B | ASP191 |
B | LEU192 |
B | GLU195 |
B | 9TY405 |
site_id | AC7 |
Number of Residues | 4 |
Details | binding site for residue MG B 402 |
Chain | Residue |
A | GLU227 |
B | ARG130 |
B | ASP191 |
B | 9TY405 |
site_id | AC8 |
Number of Residues | 1 |
Details | binding site for residue MG B 403 |
Chain | Residue |
B | NAI404 |
site_id | AC9 |
Number of Residues | 17 |
Details | binding site for residue NAI B 404 |
Chain | Residue |
B | TYR23 |
B | GLY24 |
B | ASN25 |
B | GLN26 |
B | ASN45 |
B | VAL46 |
B | TYR50 |
B | ILE78 |
B | PRO79 |
B | ASP80 |
B | VAL82 |
B | ALA106 |
B | MET131 |
B | VAL132 |
B | GLY133 |
B | MG403 |
B | 9TY405 |
site_id | AD1 |
Number of Residues | 7 |
Details | binding site for residue 9TY B 405 |
Chain | Residue |
A | GLU233 |
A | SER257 |
B | ASP191 |
B | GLU195 |
B | MG401 |
B | MG402 |
B | NAI404 |
site_id | AD2 |
Number of Residues | 4 |
Details | binding site for residue MG C 401 |
Chain | Residue |
C | ASP191 |
C | LEU192 |
C | GLU195 |
C | 9TY405 |
site_id | AD3 |
Number of Residues | 4 |
Details | binding site for residue MG C 402 |
Chain | Residue |
C | ARG130 |
C | ASP191 |
C | 9TY405 |
D | GLU227 |
site_id | AD4 |
Number of Residues | 1 |
Details | binding site for residue MG C 403 |
Chain | Residue |
C | NAI404 |
site_id | AD5 |
Number of Residues | 12 |
Details | binding site for residue NAI C 404 |
Chain | Residue |
C | GLY24 |
C | ASN25 |
C | GLN26 |
C | ASN45 |
C | VAL46 |
C | TYR50 |
C | ILE78 |
C | ASP80 |
C | ALA106 |
C | MET131 |
C | VAL132 |
C | MG403 |
site_id | AD6 |
Number of Residues | 6 |
Details | binding site for residue 9TY C 405 |
Chain | Residue |
C | ASP191 |
C | GLU195 |
C | MG401 |
C | MG402 |
D | GLU233 |
D | SER257 |
site_id | AD7 |
Number of Residues | 4 |
Details | binding site for residue MG D 401 |
Chain | Residue |
D | ASP191 |
D | LEU192 |
D | GLU195 |
D | 9TY405 |
site_id | AD8 |
Number of Residues | 4 |
Details | binding site for residue MG D 402 |
Chain | Residue |
C | GLU227 |
D | ARG130 |
D | ASP191 |
D | 9TY405 |
site_id | AD9 |
Number of Residues | 1 |
Details | binding site for residue MG D 403 |
Chain | Residue |
D | NAI404 |
site_id | AE1 |
Number of Residues | 16 |
Details | binding site for residue NAI D 404 |
Chain | Residue |
C | SER254 |
D | TYR23 |
D | GLY24 |
D | ASN25 |
D | GLN26 |
D | ASN45 |
D | VAL46 |
D | TYR50 |
D | ILE78 |
D | PRO79 |
D | ASP80 |
D | ALA106 |
D | MET131 |
D | VAL132 |
D | MG403 |
D | 9TY405 |
site_id | AE2 |
Number of Residues | 7 |
Details | binding site for residue 9TY D 405 |
Chain | Residue |
C | GLU233 |
C | SER257 |
D | ASP191 |
D | GLU195 |
D | MG401 |
D | MG402 |
D | NAI404 |
site_id | AE3 |
Number of Residues | 4 |
Details | binding site for residue MG E 401 |
Chain | Residue |
E | ASP191 |
E | LEU192 |
E | GLU195 |
E | 9TY404 |
site_id | AE4 |
Number of Residues | 1 |
Details | binding site for residue MG E 402 |
Chain | Residue |
E | NAI403 |
site_id | AE5 |
Number of Residues | 14 |
Details | binding site for residue NAI E 403 |
Chain | Residue |
E | TYR23 |
E | GLY24 |
E | ASN25 |
E | GLN26 |
E | ASN45 |
E | VAL46 |
E | TYR50 |
E | ILE78 |
E | PRO79 |
E | ASP80 |
E | ALA106 |
E | MET131 |
E | VAL132 |
E | MG402 |
site_id | AE6 |
Number of Residues | 6 |
Details | binding site for residue 9TY E 404 |
Chain | Residue |
E | ARG130 |
E | ASP191 |
E | GLU195 |
E | MG401 |
F | GLU233 |
F | MG401 |
site_id | AE7 |
Number of Residues | 4 |
Details | binding site for residue MG F 401 |
Chain | Residue |
E | ASP191 |
E | 9TY404 |
F | GLU227 |
F | GLU233 |
site_id | AE8 |
Number of Residues | 4 |
Details | binding site for residue MG F 402 |
Chain | Residue |
F | ASP191 |
F | LEU192 |
F | GLU195 |
F | 9TY406 |
site_id | AE9 |
Number of Residues | 5 |
Details | binding site for residue MG F 403 |
Chain | Residue |
E | GLU227 |
E | GLU233 |
F | ARG130 |
F | ASP191 |
F | 9TY406 |
site_id | AF1 |
Number of Residues | 1 |
Details | binding site for residue MG F 404 |
Chain | Residue |
F | NAI405 |
site_id | AF2 |
Number of Residues | 13 |
Details | binding site for residue NAI F 405 |
Chain | Residue |
F | GLY24 |
F | ASN25 |
F | GLN26 |
F | ASN45 |
F | VAL46 |
F | TYR50 |
F | ILE78 |
F | ASP80 |
F | ALA106 |
F | MET131 |
F | VAL132 |
F | MG404 |
F | 9TY406 |
site_id | AF3 |
Number of Residues | 6 |
Details | binding site for residue 9TY F 406 |
Chain | Residue |
E | GLU233 |
F | ASP191 |
F | GLU195 |
F | MG402 |
F | MG403 |
F | NAI405 |
site_id | AF4 |
Number of Residues | 4 |
Details | binding site for residue MG G 401 |
Chain | Residue |
G | ASP191 |
G | LEU192 |
G | GLU195 |
G | 9TY405 |
site_id | AF5 |
Number of Residues | 4 |
Details | binding site for residue MG G 402 |
Chain | Residue |
G | ARG130 |
G | ASP191 |
G | 9TY405 |
H | GLU227 |
site_id | AF6 |
Number of Residues | 1 |
Details | binding site for residue MG G 403 |
Chain | Residue |
G | NAI404 |
site_id | AF7 |
Number of Residues | 15 |
Details | binding site for residue NAI G 404 |
Chain | Residue |
G | GLY24 |
G | ASN25 |
G | GLN26 |
G | ASN45 |
G | VAL46 |
G | TYR50 |
G | ILE78 |
G | PRO79 |
G | ASP80 |
G | ALA106 |
G | SER107 |
G | MET131 |
G | VAL132 |
G | MG403 |
G | 9TY405 |
site_id | AF8 |
Number of Residues | 5 |
Details | binding site for residue 9TY G 405 |
Chain | Residue |
G | ASP191 |
G | GLU195 |
G | MG401 |
G | MG402 |
G | NAI404 |
site_id | AF9 |
Number of Residues | 4 |
Details | binding site for residue MG H 401 |
Chain | Residue |
H | ASP191 |
H | LEU192 |
H | GLU195 |
H | 9TY405 |
site_id | AG1 |
Number of Residues | 4 |
Details | binding site for residue MG H 402 |
Chain | Residue |
G | GLU227 |
H | ARG130 |
H | ASP191 |
H | 9TY405 |
site_id | AG2 |
Number of Residues | 1 |
Details | binding site for residue MG H 403 |
Chain | Residue |
H | NAI404 |
site_id | AG3 |
Number of Residues | 16 |
Details | binding site for residue NAI H 404 |
Chain | Residue |
H | TYR23 |
H | GLY24 |
H | ASN25 |
H | GLN26 |
H | ASN45 |
H | VAL46 |
H | TYR50 |
H | ILE78 |
H | PRO79 |
H | ASP80 |
H | ALA106 |
H | SER107 |
H | MET131 |
H | VAL132 |
H | MG403 |
H | 9TY405 |
site_id | AG4 |
Number of Residues | 6 |
Details | binding site for residue 9TY H 405 |
Chain | Residue |
H | ARG130 |
H | ASP191 |
H | GLU195 |
H | MG401 |
H | MG402 |
H | NAI404 |
site_id | AG5 |
Number of Residues | 4 |
Details | binding site for residue MG I 401 |
Chain | Residue |
I | ASP191 |
I | LEU192 |
I | GLU195 |
I | 9TY405 |
site_id | AG6 |
Number of Residues | 3 |
Details | binding site for residue MG I 402 |
Chain | Residue |
I | ASP191 |
I | 9TY405 |
J | GLU227 |
site_id | AG7 |
Number of Residues | 1 |
Details | binding site for residue MG I 403 |
Chain | Residue |
I | NAI404 |
site_id | AG8 |
Number of Residues | 15 |
Details | binding site for residue NAI I 404 |
Chain | Residue |
I | GLY24 |
I | ASN25 |
I | GLN26 |
I | ASN45 |
I | VAL46 |
I | ASP48 |
I | TYR50 |
I | ILE78 |
I | PRO79 |
I | ASP80 |
I | VAL82 |
I | ALA106 |
I | MET131 |
I | VAL132 |
I | MG403 |
site_id | AG9 |
Number of Residues | 5 |
Details | binding site for residue 9TY I 405 |
Chain | Residue |
I | ASP191 |
I | GLU195 |
I | MG401 |
I | MG402 |
J | GLU233 |
site_id | AH1 |
Number of Residues | 4 |
Details | binding site for residue MG J 401 |
Chain | Residue |
J | ASP191 |
J | LEU192 |
J | GLU195 |
J | 9TY405 |
site_id | AH2 |
Number of Residues | 3 |
Details | binding site for residue MG J 402 |
Chain | Residue |
I | GLU227 |
J | ASP191 |
J | 9TY405 |
site_id | AH3 |
Number of Residues | 1 |
Details | binding site for residue MG J 403 |
Chain | Residue |
J | NAI404 |
site_id | AH4 |
Number of Residues | 16 |
Details | binding site for residue NAI J 404 |
Chain | Residue |
J | TYR23 |
J | GLY24 |
J | ASN25 |
J | GLN26 |
J | ASN45 |
J | VAL46 |
J | TYR50 |
J | ILE78 |
J | ASP80 |
J | VAL82 |
J | ALA106 |
J | SER107 |
J | MET131 |
J | VAL132 |
J | MG403 |
J | 9TY405 |
site_id | AH5 |
Number of Residues | 6 |
Details | binding site for residue 9TY J 405 |
Chain | Residue |
I | GLU233 |
J | ASP191 |
J | GLU195 |
J | MG401 |
J | MG402 |
J | NAI404 |
site_id | AH6 |
Number of Residues | 4 |
Details | binding site for residue MG K 401 |
Chain | Residue |
K | ASP191 |
K | LEU192 |
K | GLU195 |
K | 9TY405 |
site_id | AH7 |
Number of Residues | 4 |
Details | binding site for residue MG K 402 |
Chain | Residue |
K | ARG130 |
K | ASP191 |
K | 9TY405 |
L | GLU227 |
site_id | AH8 |
Number of Residues | 1 |
Details | binding site for residue MG K 403 |
Chain | Residue |
K | NAI404 |
site_id | AH9 |
Number of Residues | 16 |
Details | binding site for residue NAI K 404 |
Chain | Residue |
K | GLY24 |
K | ASN25 |
K | GLN26 |
K | ASN45 |
K | VAL46 |
K | TYR50 |
K | LEU77 |
K | ILE78 |
K | PRO79 |
K | ASP80 |
K | VAL82 |
K | ALA106 |
K | MET131 |
K | VAL132 |
K | MG403 |
K | 9TY405 |
site_id | AI1 |
Number of Residues | 6 |
Details | binding site for residue 9TY K 405 |
Chain | Residue |
K | ASP191 |
K | GLU195 |
K | MG401 |
K | MG402 |
K | NAI404 |
L | SER257 |
site_id | AI2 |
Number of Residues | 5 |
Details | binding site for residue MG L 401 |
Chain | Residue |
K | SER257 |
L | ASP191 |
L | LEU192 |
L | GLU195 |
L | 9TY405 |
site_id | AI3 |
Number of Residues | 3 |
Details | binding site for residue MG L 402 |
Chain | Residue |
K | GLU227 |
L | ASP191 |
L | 9TY405 |
site_id | AI4 |
Number of Residues | 1 |
Details | binding site for residue MG L 403 |
Chain | Residue |
L | NAI404 |
site_id | AI5 |
Number of Residues | 18 |
Details | binding site for residue NAI L 404 |
Chain | Residue |
L | TYR23 |
L | GLY24 |
L | ASN25 |
L | GLN26 |
L | ASN45 |
L | VAL46 |
L | TYR50 |
L | ILE78 |
L | PRO79 |
L | ASP80 |
L | VAL82 |
L | VAL86 |
L | ALA106 |
L | MET131 |
L | VAL132 |
L | GLY133 |
L | MG403 |
L | 9TY405 |
site_id | AI6 |
Number of Residues | 7 |
Details | binding site for residue 9TY L 405 |
Chain | Residue |
K | GLU233 |
K | SER257 |
L | ASP191 |
L | GLU195 |
L | MG401 |
L | MG402 |
L | NAI404 |