6JD0
Structure of mutant human cathepsin L, engineered for GAG binding
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001968 | molecular_function | fibronectin binding |
| A | 0002250 | biological_process | adaptive immune response |
| A | 0004197 | molecular_function | cysteine-type endopeptidase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005518 | molecular_function | collagen binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005615 | cellular_component | extracellular space |
| A | 0005634 | cellular_component | nucleus |
| A | 0005764 | cellular_component | lysosome |
| A | 0005771 | cellular_component | multivesicular body |
| A | 0005794 | cellular_component | Golgi apparatus |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006508 | biological_process | proteolysis |
| A | 0006955 | biological_process | immune response |
| A | 0008234 | molecular_function | cysteine-type peptidase activity |
| A | 0008656 | molecular_function | cysteine-type endopeptidase activator activity involved in apoptotic process |
| A | 0016324 | cellular_component | apical plasma membrane |
| A | 0016540 | biological_process | protein autoprocessing |
| A | 0019064 | biological_process | fusion of virus membrane with host plasma membrane |
| A | 0019065 | biological_process | receptor-mediated endocytosis of virus by host cell |
| A | 0019882 | biological_process | antigen processing and presentation |
| A | 0019886 | biological_process | antigen processing and presentation of exogenous peptide antigen via MHC class II |
| A | 0030574 | biological_process | collagen catabolic process |
| A | 0031410 | cellular_component | cytoplasmic vesicle |
| A | 0031638 | biological_process | zymogen activation |
| A | 0034230 | biological_process | enkephalin processing |
| A | 0036021 | cellular_component | endolysosome lumen |
| A | 0039654 | biological_process | fusion of virus membrane with host endosome membrane |
| A | 0042393 | molecular_function | histone binding |
| A | 0042583 | cellular_component | chromaffin granule |
| A | 0043202 | cellular_component | lysosomal lumen |
| A | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| A | 0043373 | biological_process | CD4-positive, alpha-beta T cell lineage commitment |
| A | 0043394 | molecular_function | proteoglycan binding |
| A | 0046718 | biological_process | symbiont entry into host cell |
| A | 0048002 | biological_process | antigen processing and presentation of peptide antigen |
| A | 0051603 | biological_process | proteolysis involved in protein catabolic process |
| A | 0060309 | biological_process | elastin catabolic process |
| A | 0062023 | cellular_component | collagen-containing extracellular matrix |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0071682 | cellular_component | endocytic vesicle lumen |
| A | 0071888 | biological_process | macrophage apoptotic process |
| A | 0097067 | biological_process | cellular response to thyroid hormone stimulus |
| A | 0097655 | molecular_function | serpin family protein binding |
| A | 2001235 | biological_process | positive regulation of apoptotic signaling pathway |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 3 |
| Details | binding site for residue GOL A 401 |
| Chain | Residue |
| A | HIS45 |
| A | HIS54 |
| A | HOH535 |
| site_id | AC2 |
| Number of Residues | 9 |
| Details | binding site for residue GOL A 402 |
| Chain | Residue |
| A | GOL404 |
| A | HOH514 |
| A | HOH521 |
| A | LEU22 |
| A | GLY24 |
| A | TRP103 |
| A | ARG136 |
| A | ALA223 |
| A | THR224 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | binding site for residue GOL A 403 |
| Chain | Residue |
| A | VAL88 |
| A | PHE89 |
| A | GLN90 |
| site_id | AC4 |
| Number of Residues | 8 |
| Details | binding site for residue GOL A 404 |
| Chain | Residue |
| A | TYR95 |
| A | PRO98 |
| A | TRP103 |
| A | ALA223 |
| A | GOL402 |
| A | HOH514 |
| A | HOH551 |
| A | HOH576 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | binding site for residue GOL A 405 |
| Chain | Residue |
| A | ARG140 |
| A | ILE142 |
| A | VAL202 |
| A | GOL410 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 406 |
| Chain | Residue |
| A | VAL109 |
| A | THR110 |
| A | PRO111 |
| A | TYR294 |
| A | PO4426 |
| A | HOH537 |
| A | HOH555 |
| site_id | AC7 |
| Number of Residues | 11 |
| Details | binding site for residue GOL A 407 |
| Chain | Residue |
| A | ASN80 |
| A | ARG81 |
| A | LYS82 |
| A | ARG84 |
| A | TYR165 |
| A | ASP167 |
| A | TYR168 |
| A | HOH502 |
| A | HOH509 |
| A | HOH546 |
| A | HOH573 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | binding site for residue GOL A 408 |
| Chain | Residue |
| A | GLU183 |
| A | SER184 |
| A | PRO186 |
| A | PGE441 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | binding site for residue GOL A 409 |
| Chain | Residue |
| A | PRO98 |
| A | ARG99 |
| A | SER100 |
| site_id | AD1 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 410 |
| Chain | Residue |
| A | GLY177 |
| A | GLY178 |
| A | SER201 |
| A | ALA203 |
| A | ASN204 |
| A | GOL405 |
| site_id | AD2 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 411 |
| Chain | Residue |
| A | ARG104 |
| A | THR190 |
| A | GLU191 |
| A | ASN291 |
| A | LYS296 |
| A | PGE443 |
| A | HOH569 |
| site_id | AD3 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 412 |
| Chain | Residue |
| A | GLY24 |
| A | MET25 |
| A | ASN26 |
| A | GLU27 |
| A | ARG136 |
| A | HOH517 |
| site_id | AD4 |
| Number of Residues | 3 |
| Details | binding site for residue GOL A 413 |
| Chain | Residue |
| A | LYS14 |
| A | ARG302 |
| A | PGE445 |
| site_id | AD5 |
| Number of Residues | 5 |
| Details | binding site for residue GOL A 414 |
| Chain | Residue |
| A | GLN47 |
| A | GLU91 |
| A | PRO92 |
| A | LEU93 |
| A | PHE94 |
| site_id | AD6 |
| Number of Residues | 5 |
| Details | binding site for residue CL A 415 |
| Chain | Residue |
| A | SER68 |
| A | GLU69 |
| A | NA418 |
| A | POL438 |
| A | HOH639 |
| site_id | AD7 |
| Number of Residues | 2 |
| Details | binding site for residue CL A 416 |
| Chain | Residue |
| A | LYS105 |
| A | GLN117 |
| site_id | AD8 |
| Number of Residues | 2 |
| Details | binding site for residue CL A 417 |
| Chain | Residue |
| A | PRO212 |
| A | LYS213 |
| site_id | AD9 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 418 |
| Chain | Residue |
| A | THR67 |
| A | GLU69 |
| A | GLY107 |
| A | PHE135 |
| A | LEU141 |
| A | CL415 |
| site_id | AE1 |
| Number of Residues | 5 |
| Details | binding site for residue NA A 419 |
| Chain | Residue |
| A | VAL112 |
| A | LYS113 |
| A | GLY286 |
| A | PO4428 |
| A | POL435 |
| site_id | AE2 |
| Number of Residues | 5 |
| Details | binding site for residue NA A 420 |
| Chain | Residue |
| A | SER193 |
| A | CYS194 |
| A | GLU244 |
| A | NA422 |
| A | PO4424 |
| site_id | AE3 |
| Number of Residues | 3 |
| Details | binding site for residue NA A 421 |
| Chain | Residue |
| A | GLU43 |
| A | ARG50 |
| A | PO4423 |
| site_id | AE4 |
| Number of Residues | 4 |
| Details | binding site for residue NA A 422 |
| Chain | Residue |
| A | THR57 |
| A | SER193 |
| A | GLU244 |
| A | NA420 |
| site_id | AE5 |
| Number of Residues | 5 |
| Details | binding site for residue PO4 A 423 |
| Chain | Residue |
| A | ASN46 |
| A | ARG50 |
| A | NA421 |
| A | PO4425 |
| A | POL431 |
| site_id | AE6 |
| Number of Residues | 5 |
| Details | binding site for residue PO4 A 424 |
| Chain | Residue |
| A | ASP151 |
| A | LYS243 |
| A | NA420 |
| A | HOH533 |
| A | HOH602 |
| site_id | AE7 |
| Number of Residues | 2 |
| Details | binding site for residue PO4 A 425 |
| Chain | Residue |
| A | ARG50 |
| A | PO4423 |
| site_id | AE8 |
| Number of Residues | 6 |
| Details | binding site for residue PO4 A 426 |
| Chain | Residue |
| A | ARG72 |
| A | CYS161 |
| A | ASN162 |
| A | GOL406 |
| A | HOH524 |
| A | HOH542 |
| site_id | AE9 |
| Number of Residues | 3 |
| Details | binding site for residue PO4 A 427 |
| Chain | Residue |
| A | PHE248 |
| A | ARG301 |
| A | ARG302 |
| site_id | AF1 |
| Number of Residues | 6 |
| Details | binding site for residue PO4 A 428 |
| Chain | Residue |
| A | ASN114 |
| A | GLY286 |
| A | GLU287 |
| A | GLU288 |
| A | NA419 |
| A | HOH568 |
| site_id | AF2 |
| Number of Residues | 2 |
| Details | binding site for residue PO4 A 429 |
| Chain | Residue |
| A | ARG84 |
| A | LYS213 |
| site_id | AF3 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 A 430 |
| Chain | Residue |
| A | THR138 |
| A | GLY139 |
| A | ARG140 |
| A | HOH637 |
| site_id | AF4 |
| Number of Residues | 5 |
| Details | binding site for residue POL A 431 |
| Chain | Residue |
| A | ALA59 |
| A | MET60 |
| A | PO4423 |
| A | HOH520 |
| A | HOH581 |
| site_id | AF5 |
| Number of Residues | 5 |
| Details | binding site for residue POL A 432 |
| Chain | Residue |
| A | VAL34 |
| A | ASN38 |
| A | GLU237 |
| A | HOH592 |
| A | HOH625 |
| site_id | AF6 |
| Number of Residues | 3 |
| Details | binding site for residue POL A 433 |
| Chain | Residue |
| A | GLU255 |
| A | ASN303 |
| A | SER309 |
| site_id | AF7 |
| Number of Residues | 1 |
| Details | binding site for residue POL A 434 |
| Chain | Residue |
| A | LEU240 |
| site_id | AF8 |
| Number of Residues | 3 |
| Details | binding site for residue POL A 435 |
| Chain | Residue |
| A | LYS113 |
| A | ASN114 |
| A | NA419 |
| site_id | AF9 |
| Number of Residues | 4 |
| Details | binding site for residue POL A 436 |
| Chain | Residue |
| A | LEU22 |
| A | PHE94 |
| A | TYR95 |
| A | GLU96 |
| site_id | AG1 |
| Number of Residues | 6 |
| Details | binding site for residue POL A 437 |
| Chain | Residue |
| A | GLY86 |
| A | LYS87 |
| A | GLN171 |
| A | GLN174 |
| A | GLY207 |
| A | PHE208 |
| site_id | AG2 |
| Number of Residues | 4 |
| Details | binding site for residue POL A 438 |
| Chain | Residue |
| A | GLY139 |
| A | ARG140 |
| A | LEU141 |
| A | CL415 |
| site_id | AG3 |
| Number of Residues | 9 |
| Details | binding site for residue POL A 439 |
| Chain | Residue |
| A | VAL74 |
| A | MET75 |
| A | ASN76 |
| A | GLN115 |
| A | GLY119 |
| A | SER120 |
| A | SER121 |
| A | ASP258 |
| A | HIS259 |
| site_id | AG4 |
| Number of Residues | 2 |
| Details | binding site for residue POL A 440 |
| Chain | Residue |
| A | SER143 |
| A | HOH552 |
| site_id | AG5 |
| Number of Residues | 5 |
| Details | binding site for residue PGE A 441 |
| Chain | Residue |
| A | SER184 |
| A | TYR185 |
| A | LYS195 |
| A | ASN197 |
| A | GOL408 |
| site_id | AG6 |
| Number of Residues | 4 |
| Details | binding site for residue PGE A 442 |
| Chain | Residue |
| A | SER55 |
| A | ASN275 |
| A | LYS277 |
| A | ARG301 |
| site_id | AG7 |
| Number of Residues | 10 |
| Details | binding site for residue PGE A 443 |
| Chain | Residue |
| A | GLU192 |
| A | SER193 |
| A | LYS195 |
| A | TYR242 |
| A | GLU244 |
| A | GLY245 |
| A | GLU269 |
| A | ASN291 |
| A | LYS296 |
| A | GOL411 |
| site_id | AG8 |
| Number of Residues | 7 |
| Details | binding site for residue PGE A 444 |
| Chain | Residue |
| A | LEU2 |
| A | THR3 |
| A | PHE4 |
| A | HIS6 |
| A | ARG99 |
| A | EDO451 |
| A | HOH512 |
| site_id | AG9 |
| Number of Residues | 5 |
| Details | binding site for residue PGE A 445 |
| Chain | Residue |
| A | LYS82 |
| A | LYS85 |
| A | GLN156 |
| A | ARG302 |
| A | GOL413 |
| site_id | AH1 |
| Number of Residues | 4 |
| Details | binding site for residue PGE A 446 |
| Chain | Residue |
| A | ASN114 |
| A | GLY116 |
| A | GLU188 |
| A | EDO450 |
| site_id | AH2 |
| Number of Residues | 4 |
| Details | binding site for residue EOH A 447 |
| Chain | Residue |
| A | ASP233 |
| A | ASP251 |
| A | SER253 |
| A | HOH532 |
| site_id | AH3 |
| Number of Residues | 5 |
| Details | binding site for residue EOH A 448 |
| Chain | Residue |
| A | LYS16 |
| A | ARG21 |
| A | HOH522 |
| A | HOH565 |
| A | HOH656 |
| site_id | AH4 |
| Number of Residues | 8 |
| Details | binding site for residue EOH A 449 |
| Chain | Residue |
| A | VAL150 |
| A | ASP151 |
| A | GLY154 |
| A | ASN158 |
| A | GLU191 |
| A | HOH533 |
| A | HOH602 |
| A | HOH610 |
| site_id | AH5 |
| Number of Residues | 7 |
| Details | binding site for residue EDO A 450 |
| Chain | Residue |
| A | SER100 |
| A | VAL101 |
| A | TYR266 |
| A | GLY267 |
| A | PHE268 |
| A | TYR278 |
| A | PGE446 |
| site_id | AH6 |
| Number of Residues | 5 |
| Details | binding site for residue EDO A 451 |
| Chain | Residue |
| A | PHE4 |
| A | TRP12 |
| A | GLU28 |
| A | ARG32 |
| A | PGE444 |
Functional Information from PROSITE/UniProt
| site_id | PS00639 |
| Number of Residues | 11 |
| Details | THIOL_PROTEASE_HIS Eukaryotic thiol (cysteine) proteases histidine active site. LDHAVLVVGYG |
| Chain | Residue | Details |
| A | LEU257-GLY267 |
| site_id | PS00640 |
| Number of Residues | 20 |
| Details | THIOL_PROTEASE_ASN Eukaryotic thiol (cysteine) proteases asparagine active site. YWLvKNSWgeeWGnkGYVkM |
| Chain | Residue | Details |
| A | TYR278-MET297 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: ACT_SITE => ECO:0000305|PubMed:9468501 |
| Chain | Residue | Details |
| A | SER121 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: |
| Chain | Residue | Details |
| A | HIS259 | |
| A | ASN283 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | SITE: Cleavage; by autolysis => ECO:0000269|PubMed:9468501 |
| Chain | Residue | Details |
| A | PHE89 | |
| A | GLN90 | |
| A | TYR95 | |
| A | GLU96 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:12754519, ECO:0000269|PubMed:16335952, ECO:0000269|PubMed:19159218 |
| Chain | Residue | Details |
| A | ASN204 |
