6J7I
Fusion protein of heme oxygenase-1 and NADPH cytochrome P450 reductase (15aa)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003420 | biological_process | regulation of growth plate cartilage chondrocyte proliferation |
| A | 0003958 | molecular_function | NADPH-hemoprotein reductase activity |
| A | 0004128 | molecular_function | cytochrome-b5 reductase activity, acting on NAD(P)H |
| A | 0004392 | molecular_function | heme oxygenase (decyclizing) activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005783 | cellular_component | endoplasmic reticulum |
| A | 0005789 | cellular_component | endoplasmic reticulum membrane |
| A | 0005829 | cellular_component | cytosol |
| A | 0006788 | biological_process | heme oxidation |
| A | 0007584 | biological_process | response to nutrient |
| A | 0008941 | molecular_function | nitric oxide dioxygenase NAD(P)H activity |
| A | 0009055 | molecular_function | electron transfer activity |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0009437 | biological_process | carnitine metabolic process |
| A | 0009725 | biological_process | response to hormone |
| A | 0009812 | biological_process | flavonoid metabolic process |
| A | 0010181 | molecular_function | FMN binding |
| A | 0016020 | cellular_component | membrane |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0019395 | biological_process | fatty acid oxidation |
| A | 0019899 | molecular_function | enzyme binding |
| A | 0022900 | biological_process | electron transport chain |
| A | 0032332 | biological_process | positive regulation of chondrocyte differentiation |
| A | 0043066 | biological_process | negative regulation of apoptotic process |
| A | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| A | 0043602 | biological_process | nitrate catabolic process |
| A | 0045542 | biological_process | positive regulation of cholesterol biosynthetic process |
| A | 0045880 | biological_process | positive regulation of smoothened signaling pathway |
| A | 0046210 | biological_process | nitric oxide catabolic process |
| A | 0047726 | molecular_function | iron-cytochrome-c reductase activity |
| A | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| A | 0050661 | molecular_function | NADP binding |
| A | 0070988 | biological_process | demethylation |
| A | 0071371 | biological_process | cellular response to gonadotropin stimulus |
| A | 0071372 | biological_process | cellular response to follicle-stimulating hormone stimulus |
| A | 0071375 | biological_process | cellular response to peptide hormone stimulus |
| A | 0071548 | biological_process | response to dexamethasone |
| A | 0090031 | biological_process | positive regulation of steroid hormone biosynthetic process |
| A | 0090181 | biological_process | regulation of cholesterol metabolic process |
| A | 0090346 | biological_process | cellular organofluorine metabolic process |
| B | 0003420 | biological_process | regulation of growth plate cartilage chondrocyte proliferation |
| B | 0003958 | molecular_function | NADPH-hemoprotein reductase activity |
| B | 0004128 | molecular_function | cytochrome-b5 reductase activity, acting on NAD(P)H |
| B | 0004392 | molecular_function | heme oxygenase (decyclizing) activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005783 | cellular_component | endoplasmic reticulum |
| B | 0005789 | cellular_component | endoplasmic reticulum membrane |
| B | 0005829 | cellular_component | cytosol |
| B | 0006788 | biological_process | heme oxidation |
| B | 0007584 | biological_process | response to nutrient |
| B | 0008941 | molecular_function | nitric oxide dioxygenase NAD(P)H activity |
| B | 0009055 | molecular_function | electron transfer activity |
| B | 0009410 | biological_process | response to xenobiotic stimulus |
| B | 0009437 | biological_process | carnitine metabolic process |
| B | 0009725 | biological_process | response to hormone |
| B | 0009812 | biological_process | flavonoid metabolic process |
| B | 0010181 | molecular_function | FMN binding |
| B | 0016020 | cellular_component | membrane |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0019395 | biological_process | fatty acid oxidation |
| B | 0019899 | molecular_function | enzyme binding |
| B | 0022900 | biological_process | electron transport chain |
| B | 0032332 | biological_process | positive regulation of chondrocyte differentiation |
| B | 0043066 | biological_process | negative regulation of apoptotic process |
| B | 0043231 | cellular_component | intracellular membrane-bounded organelle |
| B | 0043602 | biological_process | nitrate catabolic process |
| B | 0045542 | biological_process | positive regulation of cholesterol biosynthetic process |
| B | 0045880 | biological_process | positive regulation of smoothened signaling pathway |
| B | 0046210 | biological_process | nitric oxide catabolic process |
| B | 0047726 | molecular_function | iron-cytochrome-c reductase activity |
| B | 0050660 | molecular_function | flavin adenine dinucleotide binding |
| B | 0050661 | molecular_function | NADP binding |
| B | 0070988 | biological_process | demethylation |
| B | 0071371 | biological_process | cellular response to gonadotropin stimulus |
| B | 0071372 | biological_process | cellular response to follicle-stimulating hormone stimulus |
| B | 0071375 | biological_process | cellular response to peptide hormone stimulus |
| B | 0071548 | biological_process | response to dexamethasone |
| B | 0090031 | biological_process | positive regulation of steroid hormone biosynthetic process |
| B | 0090181 | biological_process | regulation of cholesterol metabolic process |
| B | 0090346 | biological_process | cellular organofluorine metabolic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 13 |
| Details | binding site for residue HEM A 901 |
| Chain | Residue |
| A | LYS18 |
| A | LYS179 |
| A | ARG183 |
| A | PHE207 |
| A | ASN210 |
| A | HIS25 |
| A | GLU29 |
| A | TYR134 |
| A | THR135 |
| A | ARG136 |
| A | LEU138 |
| A | GLY139 |
| A | SER142 |
| site_id | AC2 |
| Number of Residues | 16 |
| Details | binding site for residue FAD A 902 |
| Chain | Residue |
| A | HIS494 |
| A | ARG599 |
| A | ARG629 |
| A | TYR630 |
| A | TYR631 |
| A | SER632 |
| A | CYS647 |
| A | ALA648 |
| A | VAL649 |
| A | VAL651 |
| A | TYR653 |
| A | GLY663 |
| A | VAL664 |
| A | ALA665 |
| A | THR666 |
| A | TRP852 |
| site_id | AC3 |
| Number of Residues | 20 |
| Details | binding site for residue FMN A 903 |
| Chain | Residue |
| A | SER265 |
| A | GLN266 |
| A | THR267 |
| A | GLY268 |
| A | THR269 |
| A | ALA270 |
| A | ALA317 |
| A | THR318 |
| A | TYR319 |
| A | GLY320 |
| A | GLY322 |
| A | LEU352 |
| A | GLY353 |
| A | ASN354 |
| A | TYR357 |
| A | HIS359 |
| A | PHE360 |
| A | ASN361 |
| A | ASP387 |
| A | LEU391 |
| site_id | AC4 |
| Number of Residues | 14 |
| Details | binding site for residue HEM B 901 |
| Chain | Residue |
| B | LYS18 |
| B | HIS25 |
| B | GLU29 |
| B | TYR134 |
| B | THR135 |
| B | ARG136 |
| B | LEU138 |
| B | GLY139 |
| B | SER142 |
| B | LYS179 |
| B | ARG183 |
| B | PHE207 |
| B | ASN210 |
| B | PHE214 |
| site_id | AC5 |
| Number of Residues | 15 |
| Details | binding site for residue FAD B 902 |
| Chain | Residue |
| B | HIS494 |
| B | ARG599 |
| B | ARG629 |
| B | TYR630 |
| B | TYR631 |
| B | SER632 |
| B | CYS647 |
| B | ALA648 |
| B | VAL649 |
| B | TYR653 |
| B | GLY663 |
| B | VAL664 |
| B | ALA665 |
| B | THR666 |
| B | TRP852 |
| site_id | AC6 |
| Number of Residues | 19 |
| Details | binding site for residue FMN B 903 |
| Chain | Residue |
| B | SER265 |
| B | GLN266 |
| B | THR267 |
| B | GLY268 |
| B | THR269 |
| B | ALA270 |
| B | ALA317 |
| B | THR318 |
| B | TYR319 |
| B | GLY320 |
| B | LEU352 |
| B | GLY353 |
| B | ASN354 |
| B | TYR357 |
| B | HIS359 |
| B | PHE360 |
| B | ASN361 |
| B | ASP387 |
| B | LEU391 |
Functional Information from PROSITE/UniProt
| site_id | PS00593 |
| Number of Residues | 11 |
| Details | HEME_OXYGENASE Heme oxygenase signature. LVAHAYTRYLG |
| Chain | Residue | Details |
| A | LEU129-GLY139 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P09601 |
| Chain | Residue | Details |
| A | ARG183 | |
| B | LYS18 | |
| B | TYR134 | |
| B | ARG183 | |
| A | LYS18 | |
| A | TYR134 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: axial binding residue => ECO:0000250|UniProtKB:P09601 |
| Chain | Residue | Details |
| A | HIS25 | |
| B | HIS25 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | SITE: Important for catalytic activity => ECO:0000250|UniProtKB:P09601 |
| Chain | Residue | Details |
| A | ASP140 | |
| B | ASP140 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P09601 |
| Chain | Residue | Details |
| A | SER229 | |
| B | SER229 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 20 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:11371558, ECO:0000269|PubMed:19171935, ECO:0000269|PubMed:21345800, ECO:0000269|PubMed:9237990, ECO:0000269|Ref.8 |
| Chain | Residue | Details |
| A | PRO714 | |
| A | ALA775 | |
| A | GLN781 | |
| B | SER265 | |
| B | ALA317 | |
| B | LEU352 | |
| B | ILE633 | |
| B | SER657 | |
| B | SER667 | |
| B | PRO714 | |
| B | ALA775 | |
| B | GLN781 | |
| A | SER265 | |
| A | ALA317 | |
| A | LEU352 | |
| A | ILE633 | |
| A | SER657 | |
| A | SER667 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:11371558, ECO:0000269|PubMed:21345800, ECO:0000269|PubMed:9237990 |
| Chain | Residue | Details |
| A | ASP387 | |
| B | ASP387 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:11371558, ECO:0000269|PubMed:19171935, ECO:0000269|PubMed:21345800, ECO:0000269|PubMed:9237990 |
| Chain | Residue | Details |
| B | HIS477 | |
| B | THR818 | |
| A | HIS477 | |
| A | THR818 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:11371558, ECO:0000269|PubMed:19171935, ECO:0000269|PubMed:21345800, ECO:0000269|Ref.8 |
| Chain | Residue | Details |
| A | LEU603 | |
| B | LEU603 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03212, ECO:0000269|PubMed:11371558, ECO:0000269|PubMed:19171935, ECO:0000269|PubMed:9237990, ECO:0000269|Ref.8 |
| Chain | Residue | Details |
| A | VAL651 | |
| B | VAL651 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 117 |
| Chain | Residue | Details |
| A | SER636 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay, radical stabiliser |
| A | ARG809 | electrostatic stabiliser, hydrogen bond donor, proton acceptor, proton donor, proton relay, steric role, van der waals interaction |
| site_id | MCSA2 |
| Number of Residues | 4 |
| Details | M-CSA 117 |
| Chain | Residue | Details |
| B | SER636 | hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor, proton relay, radical stabiliser |
| B | ARG809 | electrostatic stabiliser, hydrogen bond donor, proton acceptor, proton donor, proton relay, steric role, van der waals interaction |
