6HTD

Yeast 20S proteasome with human beta2c (S171G) in complex with 4

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000502	cellular_component	proteasome complex
A	0003674	molecular_function	molecular_function
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005839	cellular_component	proteasome core complex
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
A	0019773	cellular_component	proteasome core complex, alpha-subunit complex
A	0034515	cellular_component	proteasome storage granule
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0051603	biological_process	proteolysis involved in protein catabolic process
B	0000502	cellular_component	proteasome complex
B	0003674	molecular_function	molecular_function
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005839	cellular_component	proteasome core complex
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
B	0019773	cellular_component	proteasome core complex, alpha-subunit complex
B	0034515	cellular_component	proteasome storage granule
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0051603	biological_process	proteolysis involved in protein catabolic process
B	0080129	biological_process	proteasome core complex assembly
C	0000502	cellular_component	proteasome complex
C	0003674	molecular_function	molecular_function
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005839	cellular_component	proteasome core complex
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
C	0019773	cellular_component	proteasome core complex, alpha-subunit complex
C	0034515	cellular_component	proteasome storage granule
C	0042175	cellular_component	nuclear outer membrane-endoplasmic reticulum membrane network
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0051603	biological_process	proteolysis involved in protein catabolic process
D	0000502	cellular_component	proteasome complex
D	0003674	molecular_function	molecular_function
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005839	cellular_component	proteasome core complex
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
D	0019773	cellular_component	proteasome core complex, alpha-subunit complex
D	0034515	cellular_component	proteasome storage granule
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0051603	biological_process	proteolysis involved in protein catabolic process
E	0000502	cellular_component	proteasome complex
E	0003674	molecular_function	molecular_function
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005839	cellular_component	proteasome core complex
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
E	0019773	cellular_component	proteasome core complex, alpha-subunit complex
E	0034515	cellular_component	proteasome storage granule
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0051603	biological_process	proteolysis involved in protein catabolic process
F	0000502	cellular_component	proteasome complex
F	0003729	molecular_function	mRNA binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005839	cellular_component	proteasome core complex
F	0006511	biological_process	ubiquitin-dependent protein catabolic process
F	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
F	0019773	cellular_component	proteasome core complex, alpha-subunit complex
F	0034515	cellular_component	proteasome storage granule
F	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
F	0051603	biological_process	proteolysis involved in protein catabolic process
G	0000502	cellular_component	proteasome complex
G	0003674	molecular_function	molecular_function
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005739	cellular_component	mitochondrion
G	0005839	cellular_component	proteasome core complex
G	0006511	biological_process	ubiquitin-dependent protein catabolic process
G	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
G	0019773	cellular_component	proteasome core complex, alpha-subunit complex
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
G	0051603	biological_process	proteolysis involved in protein catabolic process
H	0004298	molecular_function	threonine-type endopeptidase activity
H	0005839	cellular_component	proteasome core complex
H	0051603	biological_process	proteolysis involved in protein catabolic process
I	0000502	cellular_component	proteasome complex
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0005839	cellular_component	proteasome core complex
I	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
I	0019774	cellular_component	proteasome core complex, beta-subunit complex
I	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
I	0051603	biological_process	proteolysis involved in protein catabolic process
I	0061133	molecular_function	endopeptidase activator activity
J	0000502	cellular_component	proteasome complex
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005737	cellular_component	cytoplasm
J	0005789	cellular_component	endoplasmic reticulum membrane
J	0005829	cellular_component	cytosol
J	0005839	cellular_component	proteasome core complex
J	0010498	biological_process	proteasomal protein catabolic process
J	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
J	0019774	cellular_component	proteasome core complex, beta-subunit complex
J	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0051603	biological_process	proteolysis involved in protein catabolic process
J	0061133	molecular_function	endopeptidase activator activity
K	0004298	molecular_function	threonine-type endopeptidase activity
K	0005839	cellular_component	proteasome core complex
K	0051603	biological_process	proteolysis involved in protein catabolic process
L	0000502	cellular_component	proteasome complex
L	0003674	molecular_function	molecular_function
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0005839	cellular_component	proteasome core complex
L	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
L	0019774	cellular_component	proteasome core complex, beta-subunit complex
L	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
L	0051603	biological_process	proteolysis involved in protein catabolic process
M	0000502	cellular_component	proteasome complex
M	0003674	molecular_function	molecular_function
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0005839	cellular_component	proteasome core complex
M	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
M	0019774	cellular_component	proteasome core complex, beta-subunit complex
M	0034515	cellular_component	proteasome storage granule
M	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
M	0043248	biological_process	proteasome assembly
M	0051603	biological_process	proteolysis involved in protein catabolic process
N	0000502	cellular_component	proteasome complex
N	0004175	molecular_function	endopeptidase activity
N	0004298	molecular_function	threonine-type endopeptidase activity
N	0005515	molecular_function	protein binding
N	0005634	cellular_component	nucleus
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0005839	cellular_component	proteasome core complex
N	0006508	biological_process	proteolysis
N	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
N	0019774	cellular_component	proteasome core complex, beta-subunit complex
N	0034515	cellular_component	proteasome storage granule
N	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
N	0051603	biological_process	proteolysis involved in protein catabolic process
O	0000502	cellular_component	proteasome complex
O	0003674	molecular_function	molecular_function
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005737	cellular_component	cytoplasm
O	0005839	cellular_component	proteasome core complex
O	0006511	biological_process	ubiquitin-dependent protein catabolic process
O	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
O	0019773	cellular_component	proteasome core complex, alpha-subunit complex
O	0034515	cellular_component	proteasome storage granule
O	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
O	0051603	biological_process	proteolysis involved in protein catabolic process
P	0000502	cellular_component	proteasome complex
P	0003674	molecular_function	molecular_function
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0005839	cellular_component	proteasome core complex
P	0006511	biological_process	ubiquitin-dependent protein catabolic process
P	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
P	0019773	cellular_component	proteasome core complex, alpha-subunit complex
P	0034515	cellular_component	proteasome storage granule
P	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
P	0051603	biological_process	proteolysis involved in protein catabolic process
P	0080129	biological_process	proteasome core complex assembly
Q	0000502	cellular_component	proteasome complex
Q	0003674	molecular_function	molecular_function
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005737	cellular_component	cytoplasm
Q	0005739	cellular_component	mitochondrion
Q	0005839	cellular_component	proteasome core complex
Q	0006511	biological_process	ubiquitin-dependent protein catabolic process
Q	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
Q	0019773	cellular_component	proteasome core complex, alpha-subunit complex
Q	0034515	cellular_component	proteasome storage granule
Q	0042175	cellular_component	nuclear outer membrane-endoplasmic reticulum membrane network
Q	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Q	0051603	biological_process	proteolysis involved in protein catabolic process
R	0000502	cellular_component	proteasome complex
R	0003674	molecular_function	molecular_function
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005737	cellular_component	cytoplasm
R	0005839	cellular_component	proteasome core complex
R	0006511	biological_process	ubiquitin-dependent protein catabolic process
R	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
R	0019773	cellular_component	proteasome core complex, alpha-subunit complex
R	0034515	cellular_component	proteasome storage granule
R	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
R	0051603	biological_process	proteolysis involved in protein catabolic process
S	0000502	cellular_component	proteasome complex
S	0003674	molecular_function	molecular_function
S	0005515	molecular_function	protein binding
S	0005634	cellular_component	nucleus
S	0005737	cellular_component	cytoplasm
S	0005839	cellular_component	proteasome core complex
S	0006511	biological_process	ubiquitin-dependent protein catabolic process
S	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
S	0019773	cellular_component	proteasome core complex, alpha-subunit complex
S	0034515	cellular_component	proteasome storage granule
S	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
S	0051603	biological_process	proteolysis involved in protein catabolic process
T	0000502	cellular_component	proteasome complex
T	0003729	molecular_function	mRNA binding
T	0005515	molecular_function	protein binding
T	0005634	cellular_component	nucleus
T	0005737	cellular_component	cytoplasm
T	0005839	cellular_component	proteasome core complex
T	0006511	biological_process	ubiquitin-dependent protein catabolic process
T	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
T	0019773	cellular_component	proteasome core complex, alpha-subunit complex
T	0034515	cellular_component	proteasome storage granule
T	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
T	0051603	biological_process	proteolysis involved in protein catabolic process
U	0000502	cellular_component	proteasome complex
U	0003674	molecular_function	molecular_function
U	0005515	molecular_function	protein binding
U	0005634	cellular_component	nucleus
U	0005737	cellular_component	cytoplasm
U	0005739	cellular_component	mitochondrion
U	0005839	cellular_component	proteasome core complex
U	0006511	biological_process	ubiquitin-dependent protein catabolic process
U	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
U	0019773	cellular_component	proteasome core complex, alpha-subunit complex
U	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
U	0051603	biological_process	proteolysis involved in protein catabolic process
V	0004298	molecular_function	threonine-type endopeptidase activity
V	0005839	cellular_component	proteasome core complex
V	0051603	biological_process	proteolysis involved in protein catabolic process
W	0000502	cellular_component	proteasome complex
W	0005515	molecular_function	protein binding
W	0005634	cellular_component	nucleus
W	0005737	cellular_component	cytoplasm
W	0005829	cellular_component	cytosol
W	0005839	cellular_component	proteasome core complex
W	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
W	0019774	cellular_component	proteasome core complex, beta-subunit complex
W	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
W	0051603	biological_process	proteolysis involved in protein catabolic process
W	0061133	molecular_function	endopeptidase activator activity
X	0000502	cellular_component	proteasome complex
X	0005515	molecular_function	protein binding
X	0005634	cellular_component	nucleus
X	0005737	cellular_component	cytoplasm
X	0005789	cellular_component	endoplasmic reticulum membrane
X	0005829	cellular_component	cytosol
X	0005839	cellular_component	proteasome core complex
X	0010498	biological_process	proteasomal protein catabolic process
X	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
X	0019774	cellular_component	proteasome core complex, beta-subunit complex
X	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
X	0051603	biological_process	proteolysis involved in protein catabolic process
X	0061133	molecular_function	endopeptidase activator activity
Y	0004298	molecular_function	threonine-type endopeptidase activity
Y	0005839	cellular_component	proteasome core complex
Y	0051603	biological_process	proteolysis involved in protein catabolic process
Z	0000502	cellular_component	proteasome complex
Z	0003674	molecular_function	molecular_function
Z	0005515	molecular_function	protein binding
Z	0005634	cellular_component	nucleus
Z	0005737	cellular_component	cytoplasm
Z	0005829	cellular_component	cytosol
Z	0005839	cellular_component	proteasome core complex
Z	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
Z	0019774	cellular_component	proteasome core complex, beta-subunit complex
Z	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Z	0051603	biological_process	proteolysis involved in protein catabolic process
a	0000502	cellular_component	proteasome complex
a	0003674	molecular_function	molecular_function
a	0005515	molecular_function	protein binding
a	0005634	cellular_component	nucleus
a	0005737	cellular_component	cytoplasm
a	0005829	cellular_component	cytosol
a	0005839	cellular_component	proteasome core complex
a	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
a	0019774	cellular_component	proteasome core complex, beta-subunit complex
a	0034515	cellular_component	proteasome storage granule
a	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
a	0043248	biological_process	proteasome assembly
a	0051603	biological_process	proteolysis involved in protein catabolic process
b	0000502	cellular_component	proteasome complex
b	0004175	molecular_function	endopeptidase activity
b	0004298	molecular_function	threonine-type endopeptidase activity
b	0005515	molecular_function	protein binding
b	0005634	cellular_component	nucleus
b	0005737	cellular_component	cytoplasm
b	0005829	cellular_component	cytosol
b	0005839	cellular_component	proteasome core complex
b	0006508	biological_process	proteolysis
b	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
b	0019774	cellular_component	proteasome core complex, beta-subunit complex
b	0034515	cellular_component	proteasome storage granule
b	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
b	0051603	biological_process	proteolysis involved in protein catabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue MG G 301

Chain	Residue
G	THR8
G	TYR119
G	ARG122
G	ALA123
G	MET125

---

site_id	AC2
Number of Residues	2
Details	binding site for residue CL G 302

Chain	Residue
G	ARG111
G	ASN114

---

site_id	AC3
Number of Residues	11
Details	binding site for residue GQH H 301

Chain	Residue
H	SER32
H	GLY45
H	GLY47
H	ALA49
H	ASP53
H	GLY128
H	SER129
I	ASP124
I	LEU125
H	THR1
H	THR21

---

site_id	AC4
Number of Residues	4
Details	binding site for residue MG I 301

Chain	Residue
I	ALA174
I	ASP177
I	SER180
I	ASP204

---

site_id	AC5
Number of Residues	13
Details	binding site for residue GQH K 301

Chain	Residue
K	THR1
K	ALA20
K	THR21
K	ALA27
K	VAL31
K	MET45
K	GLY47
K	ALA49
K	GLN53
K	SER131
K	HOH404
L	ASP126
L	GLU132

---

site_id	AC6
Number of Residues	6
Details	binding site for residue MG K 302

Chain	Residue
K	ALA165
K	HIS166
K	ASP168
K	ALA169
K	SER171
W	ASP204

---

site_id	AC7
Number of Residues	5
Details	binding site for residue MG N 201

Chain	Residue
N	ARG19
N	ILE163
N	ASP166
N	SER169
a	LEU34

---

site_id	AC8
Number of Residues	6
Details	binding site for residue SO4 N 202

Chain	Residue
N	THR1
N	SER46
N	GLY47
N	GLY128
N	SER129
N	HOH301

---

site_id	AC9
Number of Residues	2
Details	binding site for residue CL U 301

Chain	Residue
U	ARG111
U	ASN114

---

site_id	AD1
Number of Residues	5
Details	binding site for residue MG V 302

Chain	Residue
L	ASP222
V	ILE163
V	PHE164
V	ASP166
V	SER169

---

site_id	AD2
Number of Residues	4
Details	binding site for residue MG W 301

Chain	Residue
W	ALA174
W	ASP177
W	SER180
W	ASP204

---

site_id	AD3
Number of Residues	5
Details	binding site for residue MG Y 302

Chain	Residue
I	ASP204
Y	ALA165
Y	HIS166
Y	ASP168
Y	ALA169

---

site_id	AD4
Number of Residues	6
Details	binding site for residue MG Z 301

Chain	Residue
Z	ARG28
Z	THR192
Z	HIS195
Z	ILE196
Z	VAL198
Z	ASP222

---

site_id	AD5
Number of Residues	7
Details	binding site for residue SO4 b 201

Chain	Residue
b	THR1
b	SER46
b	GLY47
b	GLY128
b	SER129
b	HOH302
b	HOH305

---

site_id	AD6
Number of Residues	18
Details	binding site for Di-peptide GQH V 301 and THR V 1

Chain	Residue
V	THR2
V	ASP17
V	THR21
V	CYS31
V	SER32
V	LYS33
V	GLY45
V	ALA46
V	GLY47
V	ALA49
V	ASP53
V	GLY128
V	SER129
V	GLY130
V	GLY168
V	SER169
W	ASP124
W	LEU125

---

site_id	AD7
Number of Residues	19
Details	binding site for Di-peptide GQH Y 301 and THR Y 1

Chain	Residue
Y	THR2
Y	ASP17
Y	ALA20
Y	THR21
Y	ALA27
Y	VAL31
Y	LYS33
Y	MET45
Y	GLY47
Y	ALA49
Y	GLN53
Y	GLY130
Y	SER131
Y	GLY132
Y	TYR170
Y	SER171
Y	HOH404
Z	ASP126
Z	GLU132

---

Functional Information from PROSITE/UniProt

site_id	PS00388
Number of Residues	23
Details	PROTEASOME_ALPHA_1 Proteasome alpha-type subunits signature. YdgdtVtFSPtGRlfQVEYAleA

Chain	Residue	Details
E	TYR5-ALA27
G	TYR3-ALA25
B	TYR5-SER27
D	TYR0-ALA22
F	TYR4-ALA26
C	TYR2-ALA24
A	TYR5-ALA27

---

site_id	PS00854
Number of Residues	48
Details	PROTEASOME_BETA_1 Proteasome beta-type subunits signature. ISMkYdnGVIIAADnlgsygsllrfngve.RlipvgdntvvgisGdiSD

Chain	Residue	Details
a	ILE12-ASP59
I	VAL12-ASP58
H	ALA4-ASP51
J	LEU5-ASP52
L	LEU13-ASP60
b	MET4-ASP51
B	ILE35-ASP82
C	VAL32-ASP79
K	LEU4-ASP51

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:9087403

Chain	Residue	Details
N	THR1
b	THR1
B	LYS198
B	LYS230
P	LYS99
P	LYS198
P	LYS230

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING:

Chain	Residue	Details
K	ALA49
Y	ALA49
W	LYS69

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047

Chain	Residue	Details
G	LYS223
R	SER243
U	LYS223

---