6HCS

Crystal structure of CaM-peptide complex containing AzF at position 108

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000086	biological_process	G2/M transition of mitotic cell cycle
A	0000922	cellular_component	spindle pole
A	0002027	biological_process	regulation of heart rate
A	0005509	molecular_function	calcium ion binding
A	0005513	biological_process	detection of calcium ion
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005813	cellular_component	centrosome
A	0005819	cellular_component	spindle
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0005876	cellular_component	spindle microtubule
A	0005886	cellular_component	plasma membrane
A	0007186	biological_process	G protein-coupled receptor signaling pathway
A	0008076	cellular_component	voltage-gated potassium channel complex
A	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
A	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
A	0010856	molecular_function	adenylate cyclase activator activity
A	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
A	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
A	0016020	cellular_component	membrane
A	0016240	biological_process	autophagosome membrane docking
A	0019855	molecular_function	calcium channel inhibitor activity
A	0019901	molecular_function	protein kinase binding
A	0021762	biological_process	substantia nigra development
A	0030017	cellular_component	sarcomere
A	0031432	molecular_function	titin binding
A	0031514	cellular_component	motile cilium
A	0031954	biological_process	positive regulation of protein autophosphorylation
A	0031982	cellular_component	vesicle
A	0032465	biological_process	regulation of cytokinesis
A	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
A	0032991	cellular_component	protein-containing complex
A	0034704	cellular_component	calcium channel complex
A	0035307	biological_process	positive regulation of protein dephosphorylation
A	0035458	biological_process	cellular response to interferon-beta
A	0043209	cellular_component	myelin sheath
A	0043539	molecular_function	protein serine/threonine kinase activator activity
A	0044325	molecular_function	transmembrane transporter binding
A	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
A	0046872	molecular_function	metal ion binding
A	0048306	molecular_function	calcium-dependent protein binding
A	0050848	biological_process	regulation of calcium-mediated signaling
A	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
A	0051592	biological_process	response to calcium ion
A	0055117	biological_process	regulation of cardiac muscle contraction
A	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
A	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
A	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
A	0071346	biological_process	cellular response to type II interferon
A	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
A	0072542	molecular_function	protein phosphatase activator activity
A	0097225	cellular_component	sperm midpiece
A	0098901	biological_process	regulation of cardiac muscle cell action potential
A	0140056	biological_process	organelle localization by membrane tethering
A	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
A	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
A	1902494	cellular_component	catalytic complex
A	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
A	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering
C	0000086	biological_process	G2/M transition of mitotic cell cycle
C	0000922	cellular_component	spindle pole
C	0002027	biological_process	regulation of heart rate
C	0005509	molecular_function	calcium ion binding
C	0005513	biological_process	detection of calcium ion
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005813	cellular_component	centrosome
C	0005819	cellular_component	spindle
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0005876	cellular_component	spindle microtubule
C	0005886	cellular_component	plasma membrane
C	0007186	biological_process	G protein-coupled receptor signaling pathway
C	0008076	cellular_component	voltage-gated potassium channel complex
C	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
C	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
C	0010856	molecular_function	adenylate cyclase activator activity
C	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
C	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
C	0016020	cellular_component	membrane
C	0016240	biological_process	autophagosome membrane docking
C	0019855	molecular_function	calcium channel inhibitor activity
C	0019901	molecular_function	protein kinase binding
C	0021762	biological_process	substantia nigra development
C	0030017	cellular_component	sarcomere
C	0031432	molecular_function	titin binding
C	0031514	cellular_component	motile cilium
C	0031954	biological_process	positive regulation of protein autophosphorylation
C	0031982	cellular_component	vesicle
C	0032465	biological_process	regulation of cytokinesis
C	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
C	0032991	cellular_component	protein-containing complex
C	0034704	cellular_component	calcium channel complex
C	0035307	biological_process	positive regulation of protein dephosphorylation
C	0035458	biological_process	cellular response to interferon-beta
C	0043209	cellular_component	myelin sheath
C	0043539	molecular_function	protein serine/threonine kinase activator activity
C	0044325	molecular_function	transmembrane transporter binding
C	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
C	0046872	molecular_function	metal ion binding
C	0048306	molecular_function	calcium-dependent protein binding
C	0050848	biological_process	regulation of calcium-mediated signaling
C	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
C	0051592	biological_process	response to calcium ion
C	0055117	biological_process	regulation of cardiac muscle contraction
C	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
C	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
C	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
C	0071346	biological_process	cellular response to type II interferon
C	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
C	0072542	molecular_function	protein phosphatase activator activity
C	0097225	cellular_component	sperm midpiece
C	0098901	biological_process	regulation of cardiac muscle cell action potential
C	0140056	biological_process	organelle localization by membrane tethering
C	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
C	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
C	1902494	cellular_component	catalytic complex
C	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
C	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering
E	0000086	biological_process	G2/M transition of mitotic cell cycle
E	0000922	cellular_component	spindle pole
E	0002027	biological_process	regulation of heart rate
E	0005509	molecular_function	calcium ion binding
E	0005513	biological_process	detection of calcium ion
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005813	cellular_component	centrosome
E	0005819	cellular_component	spindle
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0005876	cellular_component	spindle microtubule
E	0005886	cellular_component	plasma membrane
E	0007186	biological_process	G protein-coupled receptor signaling pathway
E	0008076	cellular_component	voltage-gated potassium channel complex
E	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
E	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
E	0010856	molecular_function	adenylate cyclase activator activity
E	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
E	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
E	0016020	cellular_component	membrane
E	0016240	biological_process	autophagosome membrane docking
E	0019855	molecular_function	calcium channel inhibitor activity
E	0019901	molecular_function	protein kinase binding
E	0021762	biological_process	substantia nigra development
E	0030017	cellular_component	sarcomere
E	0031432	molecular_function	titin binding
E	0031514	cellular_component	motile cilium
E	0031954	biological_process	positive regulation of protein autophosphorylation
E	0031982	cellular_component	vesicle
E	0032465	biological_process	regulation of cytokinesis
E	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
E	0032991	cellular_component	protein-containing complex
E	0034704	cellular_component	calcium channel complex
E	0035307	biological_process	positive regulation of protein dephosphorylation
E	0035458	biological_process	cellular response to interferon-beta
E	0043209	cellular_component	myelin sheath
E	0043539	molecular_function	protein serine/threonine kinase activator activity
E	0044325	molecular_function	transmembrane transporter binding
E	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
E	0046872	molecular_function	metal ion binding
E	0048306	molecular_function	calcium-dependent protein binding
E	0050848	biological_process	regulation of calcium-mediated signaling
E	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
E	0051592	biological_process	response to calcium ion
E	0055117	biological_process	regulation of cardiac muscle contraction
E	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
E	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
E	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
E	0071346	biological_process	cellular response to type II interferon
E	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
E	0072542	molecular_function	protein phosphatase activator activity
E	0097225	cellular_component	sperm midpiece
E	0098901	biological_process	regulation of cardiac muscle cell action potential
E	0140056	biological_process	organelle localization by membrane tethering
E	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
E	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
E	1902494	cellular_component	catalytic complex
E	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
E	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering
G	0000086	biological_process	G2/M transition of mitotic cell cycle
G	0000922	cellular_component	spindle pole
G	0002027	biological_process	regulation of heart rate
G	0005509	molecular_function	calcium ion binding
G	0005513	biological_process	detection of calcium ion
G	0005515	molecular_function	protein binding
G	0005576	cellular_component	extracellular region
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005813	cellular_component	centrosome
G	0005819	cellular_component	spindle
G	0005829	cellular_component	cytosol
G	0005856	cellular_component	cytoskeleton
G	0005876	cellular_component	spindle microtubule
G	0005886	cellular_component	plasma membrane
G	0007186	biological_process	G protein-coupled receptor signaling pathway
G	0008076	cellular_component	voltage-gated potassium channel complex
G	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
G	0010801	biological_process	negative regulation of peptidyl-threonine phosphorylation
G	0010856	molecular_function	adenylate cyclase activator activity
G	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
G	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
G	0016020	cellular_component	membrane
G	0016240	biological_process	autophagosome membrane docking
G	0019855	molecular_function	calcium channel inhibitor activity
G	0019901	molecular_function	protein kinase binding
G	0021762	biological_process	substantia nigra development
G	0030017	cellular_component	sarcomere
G	0031432	molecular_function	titin binding
G	0031514	cellular_component	motile cilium
G	0031954	biological_process	positive regulation of protein autophosphorylation
G	0031982	cellular_component	vesicle
G	0032465	biological_process	regulation of cytokinesis
G	0032516	biological_process	positive regulation of phosphoprotein phosphatase activity
G	0032991	cellular_component	protein-containing complex
G	0034704	cellular_component	calcium channel complex
G	0035307	biological_process	positive regulation of protein dephosphorylation
G	0035458	biological_process	cellular response to interferon-beta
G	0043209	cellular_component	myelin sheath
G	0043539	molecular_function	protein serine/threonine kinase activator activity
G	0044325	molecular_function	transmembrane transporter binding
G	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
G	0046872	molecular_function	metal ion binding
G	0048306	molecular_function	calcium-dependent protein binding
G	0050848	biological_process	regulation of calcium-mediated signaling
G	0051343	biological_process	positive regulation of cyclic-nucleotide phosphodiesterase activity
G	0051592	biological_process	response to calcium ion
G	0055117	biological_process	regulation of cardiac muscle contraction
G	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
G	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
G	0060316	biological_process	positive regulation of ryanodine-sensitive calcium-release channel activity
G	0071346	biological_process	cellular response to type II interferon
G	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
G	0072542	molecular_function	protein phosphatase activator activity
G	0097225	cellular_component	sperm midpiece
G	0098901	biological_process	regulation of cardiac muscle cell action potential
G	0140056	biological_process	organelle localization by membrane tethering
G	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
G	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
G	1902494	cellular_component	catalytic complex
G	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
G	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue CA A 201

Chain	Residue
A	ASP20
A	ASP22
A	ASP24
A	THR26
A	GLU31
A	HOH342

---

site_id	AC2
Number of Residues	6
Details	binding site for residue CA A 202

Chain	Residue
A	THR62
A	GLU67
A	HOH312
A	ASP56
A	ASP58
A	ASN60

---

site_id	AC3
Number of Residues	6
Details	binding site for residue CA A 203

Chain	Residue
A	ASP93
A	ASP95
A	ASN97
A	TYR99
A	GLU104
A	HOH313

---

site_id	AC4
Number of Residues	6
Details	binding site for residue CA A 204

Chain	Residue
A	ASP129
A	ASP131
A	ASP133
A	GLN135
A	GLU140
A	HOH311

---

site_id	AC5
Number of Residues	6
Details	binding site for residue CA C 201

Chain	Residue
C	ASP20
C	ASP22
C	ASP24
C	THR26
C	GLU31
C	HOH320

---

site_id	AC6
Number of Residues	7
Details	binding site for residue CA C 202

Chain	Residue
C	ASP56
C	ASP58
C	ASN60
C	THR62
C	GLU67
C	HOH307
C	HOH347

---

site_id	AC7
Number of Residues	6
Details	binding site for residue CA C 203

Chain	Residue
C	ASP93
C	ASP95
C	ASN97
C	TYR99
C	GLU104
C	HOH312

---

site_id	AC8
Number of Residues	6
Details	binding site for residue CA C 204

Chain	Residue
C	ASP129
C	ASP131
C	ASP133
C	GLN135
C	GLU140
C	HOH306

---

site_id	AC9
Number of Residues	5
Details	binding site for residue CA E 201

Chain	Residue
E	ASP129
E	ASP131
E	ASP133
E	GLN135
E	GLU140

---

site_id	AD1
Number of Residues	5
Details	binding site for residue CA E 202

Chain	Residue
E	ASP93
E	ASP95
E	ASN97
E	TYR99
E	GLU104

---

site_id	AD2
Number of Residues	5
Details	binding site for residue CA E 203

Chain	Residue
E	ASP56
E	ASP58
E	ASN60
E	THR62
E	GLU67

---

site_id	AD3
Number of Residues	5
Details	binding site for residue CA E 204

Chain	Residue
E	ASP20
E	ASP22
E	ASP24
E	THR26
E	GLU31

---

site_id	AD4
Number of Residues	5
Details	binding site for residue CA G 201

Chain	Residue
G	ASP56
G	ASP58
G	ASN60
G	THR62
G	GLU67

---

site_id	AD5
Number of Residues	5
Details	binding site for residue CA G 202

Chain	Residue
G	ASP93
G	ASP95
G	ASN97
G	TYR99
G	GLU104

---

site_id	AD6
Number of Residues	5
Details	binding site for residue CA G 203

Chain	Residue
G	ASP129
G	ASP131
G	ASP133
G	GLN135
G	GLU140

---

site_id	AD7
Number of Residues	5
Details	binding site for residue CA G 204

Chain	Residue
G	ASP20
G	ASP22
G	ASP24
G	THR26
G	GLU31

---

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL

Chain	Residue	Details
A	ASP20-LEU32
A	ASP56-PHE68
A	ASP93-LEU105
A	ASP129-PHE141

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	MOD_RES: Phosphothreonine; by autocatalysis => ECO:0000250

Chain	Residue	Details
B	THR305
A	GLU67
C	ASP20
C	ASP22
C	ASP24
C	THR26
C	GLU31
C	ASP56
C	ASP58
C	ASN60
C	THR62
B	THR306
C	GLU67
E	ASP20
E	ASP22
E	ASP24
E	THR26
E	GLU31
E	ASP56
E	ASP58
E	ASN60
E	THR62
D	THR305
E	GLU67
G	ASP20
G	ASP22
G	ASP24
G	THR26
G	GLU31
G	ASP56
G	ASP58
G	ASN60
G	THR62
D	THR306
G	GLU67
F	THR305
F	THR306
H	THR305
H	THR306
A	THR62

---

site_id	SWS_FT_FI2
Number of Residues	40
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00448, ECO:0000269|PubMed:1474585, ECO:0000269|PubMed:27564677, ECO:0007744|PDB:1CLL, ECO:0007744|PDB:5J03

Chain	Residue	Details
A	ASP93
A	GLU140
C	ASP93
C	ASP95
C	ASN97
C	TYR99
C	GLU104
C	ASP129
C	ASP131
C	ASP133
C	GLN135
A	ASP95
C	GLU140
E	ASP93
E	ASP95
E	ASN97
E	TYR99
E	GLU104
E	ASP129
E	ASP131
E	ASP133
E	GLN135
A	ASN97
E	GLU140
G	ASP93
G	ASP95
G	ASN97
G	TYR99
G	GLU104
G	ASP129
G	ASP131
G	ASP133
G	GLN135
A	TYR99
G	GLU140
A	GLU104
A	ASP129
A	ASP131
A	ASP133
A	GLN135

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: N-acetylalanine => ECO:0000269|PubMed:7093203, ECO:0000269|Ref.7, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378, ECO:0007744|PubMed:25944712

Chain	Residue	Details
A	ALA1
C	ALA1
E	ALA1
G	ALA1

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: N6-acetyllysine; alternate => ECO:0007744|PubMed:19608861

Chain	Residue	Details
A	LYS21
C	LYS21
E	LYS21
G	LYS21

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: Phosphothreonine; by CaMK4 => ECO:0000250|UniProtKB:P0DP29

Chain	Residue	Details
A	THR44
C	THR44
E	THR44
G	THR44

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER81
C	SER81
E	SER81
G	SER81

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
A	LYS94
C	LYS94
E	LYS94
G	LYS94

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:19690332

Chain	Residue	Details
A	TYR99
C	TYR99
E	TYR99
G	TYR99

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569

Chain	Residue	Details
A	SER101
C	SER101
E	SER101
G	SER101

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:24275569

Chain	Residue	Details
A	THR110
C	THR110
E	THR110
G	THR110

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	MOD_RES: N6-methyllysine; alternate => ECO:0007744|PubMed:24129315

Chain	Residue	Details
A	LYS115
C	LYS115
E	LYS115
G	LYS115

---

site_id	SWS_FT_FI12
Number of Residues	4
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:19690332

Chain	Residue	Details
A	TYR138
C	TYR138
E	TYR138
G	TYR138

---

site_id	SWS_FT_FI13
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000250|UniProtKB:P62157

Chain	Residue	Details
A	LYS21
C	LYS21
E	LYS21
G	LYS21

---