Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6GYS

Cryo-EM structure of the CBF3-CEN3 complex of the budding yeast kinetochore

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000775	cellular_component	chromosome, centromeric region
A	0000776	cellular_component	kinetochore
A	0000921	biological_process	septin ring assembly
A	0003677	molecular_function	DNA binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005694	cellular_component	chromosome
A	0008301	molecular_function	DNA binding, bending
A	0031518	cellular_component	CBF3 complex
A	0051382	biological_process	kinetochore assembly
B	0000775	cellular_component	chromosome, centromeric region
B	0000776	cellular_component	kinetochore
B	0000921	biological_process	septin ring assembly
B	0000981	molecular_function	DNA-binding transcription factor activity, RNA polymerase II-specific
B	0003677	molecular_function	DNA binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005694	cellular_component	chromosome
B	0006355	biological_process	regulation of DNA-templated transcription
B	0006357	biological_process	regulation of transcription by RNA polymerase II
B	0007094	biological_process	mitotic spindle assembly checkpoint signaling
B	0008270	molecular_function	zinc ion binding
B	0008301	molecular_function	DNA binding, bending
B	0019237	molecular_function	centromeric DNA binding
B	0031518	cellular_component	CBF3 complex
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding
B	0051382	biological_process	kinetochore assembly
C	0000775	cellular_component	chromosome, centromeric region
C	0000776	cellular_component	kinetochore
C	0000921	biological_process	septin ring assembly
C	0000981	molecular_function	DNA-binding transcription factor activity, RNA polymerase II-specific
C	0003677	molecular_function	DNA binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005694	cellular_component	chromosome
C	0006355	biological_process	regulation of DNA-templated transcription
C	0006357	biological_process	regulation of transcription by RNA polymerase II
C	0007094	biological_process	mitotic spindle assembly checkpoint signaling
C	0008270	molecular_function	zinc ion binding
C	0008301	molecular_function	DNA binding, bending
C	0019237	molecular_function	centromeric DNA binding
C	0031518	cellular_component	CBF3 complex
C	0042802	molecular_function	identical protein binding
C	0046872	molecular_function	metal ion binding
C	0051382	biological_process	kinetochore assembly
D	0000082	biological_process	G1/S transition of mitotic cell cycle
D	0000086	biological_process	G2/M transition of mitotic cell cycle
D	0000278	biological_process	mitotic cell cycle
D	0000409	biological_process	regulation of transcription by galactose
D	0000775	cellular_component	chromosome, centromeric region
D	0000776	cellular_component	kinetochore
D	0000781	cellular_component	chromosome, telomeric region
D	0000921	biological_process	septin ring assembly
D	0003677	molecular_function	DNA binding
D	0003688	molecular_function	DNA replication origin binding
D	0004842	molecular_function	ubiquitin-protein transferase activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005694	cellular_component	chromosome
D	0005737	cellular_component	cytoplasm
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0007035	biological_process	vacuolar acidification
D	0007096	biological_process	regulation of exit from mitosis
D	0007346	biological_process	regulation of mitotic cell cycle
D	0008053	biological_process	mitochondrial fusion
D	0010458	biological_process	exit from mitosis
D	0010828	biological_process	positive regulation of glucose transmembrane transport
D	0012505	cellular_component	endomembrane system
D	0016567	biological_process	protein ubiquitination
D	0019005	cellular_component	SCF ubiquitin ligase complex
D	0019222	biological_process	regulation of metabolic process
D	0030466	biological_process	silent mating-type cassette heterochromatin formation
D	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
D	0031335	biological_process	regulation of sulfur amino acid metabolic process
D	0031509	biological_process	subtelomeric heterochromatin formation
D	0031518	cellular_component	CBF3 complex
D	0031573	biological_process	mitotic intra-S DNA damage checkpoint signaling
D	0043254	biological_process	regulation of protein-containing complex assembly
D	0043291	cellular_component	RAVE complex
D	0045116	biological_process	protein neddylation
D	0051382	biological_process	kinetochore assembly
D	0061630	molecular_function	ubiquitin protein ligase activity
D	0065003	biological_process	protein-containing complex assembly
D	0070072	biological_process	vacuolar proton-transporting V-type ATPase complex assembly
D	0071406	biological_process	cellular response to methylmercury
D	0097602	molecular_function	cullin family protein binding
D	2000766	biological_process	negative regulation of cytoplasmic translation
E	0000022	biological_process	mitotic spindle elongation
E	0000775	cellular_component	chromosome, centromeric region
E	0000776	cellular_component	kinetochore
E	0000779	cellular_component	condensed chromosome, centromeric region
E	0000921	biological_process	septin ring assembly
E	0003677	molecular_function	DNA binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005694	cellular_component	chromosome
E	0005737	cellular_component	cytoplasm
E	0005816	cellular_component	spindle pole body
E	0005819	cellular_component	spindle
E	0005856	cellular_component	cytoskeleton
E	0007059	biological_process	chromosome segregation
E	0008301	molecular_function	DNA binding, bending
E	0019237	molecular_function	centromeric DNA binding
E	0031518	cellular_component	CBF3 complex
E	0051233	cellular_component	spindle midzone
E	0051382	biological_process	kinetochore assembly
H	0000775	cellular_component	chromosome, centromeric region
H	0000776	cellular_component	kinetochore
H	0000921	biological_process	septin ring assembly
H	0003677	molecular_function	DNA binding
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005694	cellular_component	chromosome
H	0008301	molecular_function	DNA binding, bending
H	0031518	cellular_component	CBF3 complex
H	0051382	biological_process	kinetochore assembly
I	0000775	cellular_component	chromosome, centromeric region
I	0000776	cellular_component	kinetochore
I	0000921	biological_process	septin ring assembly
I	0000981	molecular_function	DNA-binding transcription factor activity, RNA polymerase II-specific
I	0003677	molecular_function	DNA binding
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005694	cellular_component	chromosome
I	0006355	biological_process	regulation of DNA-templated transcription
I	0006357	biological_process	regulation of transcription by RNA polymerase II
I	0007094	biological_process	mitotic spindle assembly checkpoint signaling
I	0008270	molecular_function	zinc ion binding
I	0008301	molecular_function	DNA binding, bending
I	0019237	molecular_function	centromeric DNA binding
I	0031518	cellular_component	CBF3 complex
I	0042802	molecular_function	identical protein binding
I	0046872	molecular_function	metal ion binding
I	0051382	biological_process	kinetochore assembly
J	0000775	cellular_component	chromosome, centromeric region
J	0000776	cellular_component	kinetochore
J	0000921	biological_process	septin ring assembly
J	0000981	molecular_function	DNA-binding transcription factor activity, RNA polymerase II-specific
J	0003677	molecular_function	DNA binding
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005694	cellular_component	chromosome
J	0006355	biological_process	regulation of DNA-templated transcription
J	0006357	biological_process	regulation of transcription by RNA polymerase II
J	0007094	biological_process	mitotic spindle assembly checkpoint signaling
J	0008270	molecular_function	zinc ion binding
J	0008301	molecular_function	DNA binding, bending
J	0019237	molecular_function	centromeric DNA binding
J	0031518	cellular_component	CBF3 complex
J	0042802	molecular_function	identical protein binding
J	0046872	molecular_function	metal ion binding
J	0051382	biological_process	kinetochore assembly
K	0000082	biological_process	G1/S transition of mitotic cell cycle
K	0000086	biological_process	G2/M transition of mitotic cell cycle
K	0000278	biological_process	mitotic cell cycle
K	0000409	biological_process	regulation of transcription by galactose
K	0000775	cellular_component	chromosome, centromeric region
K	0000776	cellular_component	kinetochore
K	0000781	cellular_component	chromosome, telomeric region
K	0000921	biological_process	septin ring assembly
K	0003677	molecular_function	DNA binding
K	0003688	molecular_function	DNA replication origin binding
K	0004842	molecular_function	ubiquitin-protein transferase activity
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005694	cellular_component	chromosome
K	0005737	cellular_component	cytoplasm
K	0006511	biological_process	ubiquitin-dependent protein catabolic process
K	0007035	biological_process	vacuolar acidification
K	0007096	biological_process	regulation of exit from mitosis
K	0007346	biological_process	regulation of mitotic cell cycle
K	0008053	biological_process	mitochondrial fusion
K	0010458	biological_process	exit from mitosis
K	0010828	biological_process	positive regulation of glucose transmembrane transport
K	0012505	cellular_component	endomembrane system
K	0016567	biological_process	protein ubiquitination
K	0019005	cellular_component	SCF ubiquitin ligase complex
K	0019222	biological_process	regulation of metabolic process
K	0030466	biological_process	silent mating-type cassette heterochromatin formation
K	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
K	0031335	biological_process	regulation of sulfur amino acid metabolic process
K	0031509	biological_process	subtelomeric heterochromatin formation
K	0031518	cellular_component	CBF3 complex
K	0031573	biological_process	mitotic intra-S DNA damage checkpoint signaling
K	0043254	biological_process	regulation of protein-containing complex assembly
K	0043291	cellular_component	RAVE complex
K	0045116	biological_process	protein neddylation
K	0051382	biological_process	kinetochore assembly
K	0061630	molecular_function	ubiquitin protein ligase activity
K	0065003	biological_process	protein-containing complex assembly
K	0070072	biological_process	vacuolar proton-transporting V-type ATPase complex assembly
K	0071406	biological_process	cellular response to methylmercury
K	0097602	molecular_function	cullin family protein binding
K	2000766	biological_process	negative regulation of cytoplasmic translation
L	0000022	biological_process	mitotic spindle elongation
L	0000775	cellular_component	chromosome, centromeric region
L	0000776	cellular_component	kinetochore
L	0000779	cellular_component	condensed chromosome, centromeric region
L	0000921	biological_process	septin ring assembly
L	0003677	molecular_function	DNA binding
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005694	cellular_component	chromosome
L	0005737	cellular_component	cytoplasm
L	0005816	cellular_component	spindle pole body
L	0005819	cellular_component	spindle
L	0005856	cellular_component	cytoskeleton
L	0007059	biological_process	chromosome segregation
L	0008301	molecular_function	DNA binding, bending
L	0019237	molecular_function	centromeric DNA binding
L	0031518	cellular_component	CBF3 complex
L	0051233	cellular_component	spindle midzone
L	0051382	biological_process	kinetochore assembly

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue ZN B 701

Chain	Residue
B	CYS14
B	CYS17
B	CYS24
B	CYS30
B	ZN702

site_id	AC2
Number of Residues	6
Details	binding site for residue ZN B 702

Chain	Residue
B	CYS42
B	ZN701
B	CYS14
B	VAL16
B	CYS30
B	CYS33

site_id	AC3
Number of Residues	6
Details	binding site for residue ZN C 701

Chain	Residue
C	CYS14
C	CYS17
C	CYS24
C	CYS30
C	ASN32
C	ZN702

site_id	AC4
Number of Residues	6
Details	binding site for residue ZN C 702

Chain	Residue
C	CYS14
C	VAL16
C	CYS30
C	CYS33
C	CYS42
C	ZN701

site_id	AC5
Number of Residues	5
Details	binding site for residue ZN I 701

Chain	Residue
I	CYS17
I	ARG20
I	CYS30
I	CYS33
I	ZN702

site_id	AC6
Number of Residues	6
Details	binding site for residue ZN I 702

Chain	Residue
I	CYS14
I	CYS17
I	CYS30
I	CYS33
I	CYS42
I	ZN701

site_id	AC7
Number of Residues	6
Details	binding site for residue ZN J 701

Chain	Residue
J	CYS14
J	CYS17
J	VAL22
J	ASP25
J	CYS30
J	ZN702

site_id	AC8
Number of Residues	5
Details	binding site for residue ZN J 702

Chain	Residue
J	CYS14
J	PRO29
J	CYS30
J	CYS42
J	ZN701

Functional Information from PROSITE/UniProt

site_id	PS00463
Number of Residues	30
Details	ZN2_CY6_FUNGAL_1 Zn(2)-C6 fungal-type DNA-binding domain signature. PCsvCTrrKvkCdrmip.......CgnCrkrgqdseC

Chain	Residue	Details
B	PRO13-CYS42

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18407956

Chain	Residue	Details
E	SER566
L	SER566
I	CYS14-CYS42
J	CYS14-CYS42

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0007744\|PubMed:19779198

Chain	Residue	Details
E	TYR706
L	TYR706
I	SER575
J	SER575

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)