6G60
Choline sulfatase from Ensifer (Sinorhizobium) meliloti cocrystalized with choline
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0008484 | molecular_function | sulfuric ester hydrolase activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0042425 | biological_process | choline biosynthetic process |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0047753 | molecular_function | choline-sulfatase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0008484 | molecular_function | sulfuric ester hydrolase activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0042425 | biological_process | choline biosynthetic process |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0047753 | molecular_function | choline-sulfatase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0008484 | molecular_function | sulfuric ester hydrolase activity |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0042425 | biological_process | choline biosynthetic process |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0047753 | molecular_function | choline-sulfatase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0008484 | molecular_function | sulfuric ester hydrolase activity |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0042425 | biological_process | choline biosynthetic process |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0047753 | molecular_function | choline-sulfatase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | binding site for residue CHT A 601 |
| Chain | Residue |
| A | ASN75 |
| A | TYR123 |
| A | TRP129 |
| A | TRP143 |
| A | HIS145 |
| A | GLU386 |
| A | LEU499 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | binding site for residue MG A 602 |
| Chain | Residue |
| A | ASP296 |
| A | HIS297 |
| A | ASP14 |
| A | DDZ54 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 A 603 |
| Chain | Residue |
| A | ARG445 |
| A | TRP449 |
| A | MET451 |
| A | GLU452 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 A 604 |
| Chain | Residue |
| A | HIS333 |
| A | LEU334 |
| A | THR335 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | binding site for residue CHT B 601 |
| Chain | Residue |
| B | ASN75 |
| B | TYR123 |
| B | TRP129 |
| B | TRP143 |
| B | HIS145 |
| B | HIS201 |
| B | GLU386 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue MG B 602 |
| Chain | Residue |
| B | ASP14 |
| B | DDZ54 |
| B | ASP296 |
| B | HIS297 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 B 603 |
| Chain | Residue |
| B | HIS333 |
| B | LEU334 |
| B | THR335 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 B 604 |
| Chain | Residue |
| B | ARG71 |
| B | HOH703 |
| B | HOH739 |
| D | ARG71 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 B 605 |
| Chain | Residue |
| B | GLN435 |
| B | GLN435 |
| B | LEU438 |
| B | LEU438 |
| B | ARG442 |
| B | ARG442 |
| site_id | AD1 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 B 606 |
| Chain | Residue |
| B | GLU182 |
| B | ARG189 |
| B | HOH844 |
| site_id | AD2 |
| Number of Residues | 8 |
| Details | binding site for residue CHT C 601 |
| Chain | Residue |
| C | ASN75 |
| C | TYR123 |
| C | TRP129 |
| C | TRP143 |
| C | HIS145 |
| C | HIS201 |
| C | GLU386 |
| C | LEU499 |
| site_id | AD3 |
| Number of Residues | 4 |
| Details | binding site for residue MG C 602 |
| Chain | Residue |
| C | ASP14 |
| C | DDZ54 |
| C | ASP296 |
| C | HIS297 |
| site_id | AD4 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 C 603 |
| Chain | Residue |
| A | ARG71 |
| C | ARG71 |
| C | HOH713 |
| C | HOH728 |
| site_id | AD5 |
| Number of Residues | 8 |
| Details | binding site for residue CHT D 1000 |
| Chain | Residue |
| D | DDZ54 |
| D | ASN75 |
| D | TYR123 |
| D | TRP129 |
| D | TRP143 |
| D | HIS145 |
| D | HIS201 |
| D | LYS309 |
| site_id | AD6 |
| Number of Residues | 5 |
| Details | binding site for residue MG D 1001 |
| Chain | Residue |
| D | ASP14 |
| D | DDZ54 |
| D | LYS102 |
| D | ASP296 |
| D | HIS297 |
| site_id | AD7 |
| Number of Residues | 15 |
| Details | binding site for Di-peptide LEU B 53 and DDZ B 54 |
| Chain | Residue |
| B | ASP14 |
| B | SER51 |
| B | PRO52 |
| B | ALA55 |
| B | PRO56 |
| B | ALA57 |
| B | ARG58 |
| B | ASN75 |
| B | LYS102 |
| B | HIS104 |
| B | ASP296 |
| B | HIS297 |
| B | ALA385 |
| B | MG602 |
| B | HOH760 |
| site_id | AD8 |
| Number of Residues | 14 |
| Details | binding site for Di-peptide DDZ B 54 and ALA B 55 |
| Chain | Residue |
| B | HIS297 |
| B | MG602 |
| B | HOH760 |
| B | HOH850 |
| B | ASP14 |
| B | LEU53 |
| B | PRO56 |
| B | ALA57 |
| B | ARG58 |
| B | ALA59 |
| B | ASN75 |
| B | LYS102 |
| B | HIS104 |
| B | ASP296 |
| site_id | AD9 |
| Number of Residues | 14 |
| Details | binding site for Di-peptide LEU C 53 and DDZ C 54 |
| Chain | Residue |
| C | SER51 |
| C | PRO52 |
| C | ALA55 |
| C | PRO56 |
| C | ALA57 |
| C | ARG58 |
| C | ASN75 |
| C | LYS102 |
| C | HIS104 |
| C | ASP296 |
| C | HIS297 |
| C | ALA385 |
| C | MG602 |
| C | HOH808 |
| site_id | AE1 |
| Number of Residues | 14 |
| Details | binding site for Di-peptide DDZ C 54 and ALA C 55 |
| Chain | Residue |
| C | LEU53 |
| C | PRO56 |
| C | ALA57 |
| C | ARG58 |
| C | ALA59 |
| C | ASP74 |
| C | ASN75 |
| C | LYS102 |
| C | HIS104 |
| C | ASP296 |
| C | HIS297 |
| C | MG602 |
| C | HOH808 |
| C | HOH886 |
| site_id | AE2 |
| Number of Residues | 13 |
| Details | binding site for Di-peptide LEU D 53 and DDZ D 54 |
| Chain | Residue |
| D | SER51 |
| D | PRO52 |
| D | ALA55 |
| D | PRO56 |
| D | ALA57 |
| D | ARG58 |
| D | ASN75 |
| D | LYS102 |
| D | HIS104 |
| D | HIS297 |
| D | ALA385 |
| D | CHT1000 |
| D | MG1001 |
| site_id | AE3 |
| Number of Residues | 12 |
| Details | binding site for Di-peptide DDZ D 54 and ALA D 55 |
| Chain | Residue |
| D | LEU53 |
| D | PRO56 |
| D | ALA57 |
| D | ARG58 |
| D | ALA59 |
| D | ASN75 |
| D | LYS102 |
| D | HIS104 |
| D | HIS297 |
| D | CHT1000 |
| D | MG1001 |
| D | HOH1215 |
Functional Information from PROSITE/UniProt
| site_id | PS00149 |
| Number of Residues | 11 |
| Details | SULFATASE_2 Sulfatases signature 2. GYyTalSGK.MH |
| Chain | Residue | Details |
| A | GLY94-HIS104 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"UniProtKB","id":"P15289","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Active site: {"evidences":[{"source":"UniProtKB","id":"P15289","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 16 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | Binding site: {"description":"via 3-oxoalanine","evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | Modified residue: {"description":"3-oxoalanine (Cys)","evidences":[{"source":"UniProtKB","id":"P15289","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
