6FOP

Glycoside hydrolase family 81 from Clostridium thermocellum (CtLam81A), Mutant E515A

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000272	biological_process	polysaccharide catabolic process
A	0000287	molecular_function	magnesium ion binding
A	0005509	molecular_function	calcium ion binding
A	0005576	cellular_component	extracellular region
A	0016151	molecular_function	nickel cation binding
A	0016787	molecular_function	hydrolase activity
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0042973	molecular_function	glucan endo-1,3-beta-D-glucosidase activity
A	0046872	molecular_function	metal ion binding
A	0052861	molecular_function	endo-1,3(4)-beta-glucanase activity
A	0071555	biological_process	cell wall organization

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	binding site for residue CA A 801

Chain	Residue
A	LEU490
A	ARG491
A	PHE493
A	HOH961
A	HOH1130
A	HOH1139
A	HOH1384

---

site_id	AC2
Number of Residues	5
Details	binding site for residue CA A 802

Chain	Residue
A	GLU409
A	LYS412
A	CL819
A	ASP401
A	THR404

---

site_id	AC3
Number of Residues	6
Details	binding site for residue CA A 803

Chain	Residue
A	ASP401
A	GLU409
A	HOH1079
A	HOH1458
A	HOH1466
A	HOH1478

---

site_id	AC4
Number of Residues	5
Details	binding site for residue MPD A 804

Chain	Residue
A	THR410
A	GLU595
A	MPD806
A	HOH1040
A	HOH1217

---

site_id	AC5
Number of Residues	6
Details	binding site for residue MPD A 805

Chain	Residue
A	TYR363
A	LYS367
A	SER516
A	TRP637
A	HOH1026
A	HOH1046

---

site_id	AC6
Number of Residues	7
Details	binding site for residue MPD A 806

Chain	Residue
A	TYR363
A	TYR430
A	ASP438
A	TRP588
A	MPD804
A	HOH1026
A	HOH1046

---

site_id	AC7
Number of Residues	6
Details	binding site for residue MPD A 807

Chain	Residue
A	PRO377
A	GLU380
A	ARG456
A	TYR710
A	HOH957
A	HOH1459

---

site_id	AC8
Number of Residues	5
Details	binding site for residue MPD A 808

Chain	Residue
A	THR410
A	TYR430
A	HOH1010
A	HOH1273
A	HOH1560

---

site_id	AC9
Number of Residues	6
Details	binding site for residue MPD A 809

Chain	Residue
A	GLY40
A	LYS42
A	TRP588
A	HOH907
A	HOH1211
A	HOH1251

---

site_id	AD1
Number of Residues	3
Details	binding site for residue MPD A 810

Chain	Residue
A	TYR416
A	SER418
A	THR485

---

site_id	AD2
Number of Residues	5
Details	binding site for residue MPD A 811

Chain	Residue
A	ASN344
A	LEU382
A	GLY383
A	ASN384
A	CL817

---

site_id	AD3
Number of Residues	2
Details	binding site for residue MPD A 812

Chain	Residue
A	TYR178
A	THR238

---

site_id	AD4
Number of Residues	7
Details	binding site for residue MPD A 813

Chain	Residue
A	THR260
A	GLU261
A	THR690
A	ALA691
A	THR720
A	PHE721
A	SER722

---

site_id	AD5
Number of Residues	6
Details	binding site for residue MPD A 814

Chain	Residue
A	LYS582
A	GLU586
A	TRP588
A	HOH1045
A	HOH1147
A	HOH1449

---

site_id	AD6
Number of Residues	8
Details	binding site for residue MPD A 815

Chain	Residue
A	TYR302
A	ARG705
A	TYR707
A	ASP723
A	GLY739
A	SER740
A	HOH1244
A	HOH1255

---

site_id	AD7
Number of Residues	5
Details	binding site for residue CL A 816

Chain	Residue
A	TYR178
A	GLN184
A	CYS185
A	LYS241
A	HOH1429

---

site_id	AD8
Number of Residues	4
Details	binding site for residue CL A 817

Chain	Residue
A	ASN344
A	ASN348
A	GLN459
A	MPD811

---

site_id	AD9
Number of Residues	2
Details	binding site for residue CL A 818

Chain	Residue
A	GLY277
A	GLY277

---

site_id	AE1
Number of Residues	6
Details	binding site for residue CL A 819

Chain	Residue
A	CA802
A	HOH1478
A	ASP401
A	THR404
A	LYS406
A	GLU409

---

site_id	AE2
Number of Residues	3
Details	binding site for residue NA A 820

Chain	Residue
A	THR592
A	ASN593
A	HOH1443

---

site_id	AE3
Number of Residues	3
Details	binding site for residue NA A 821

Chain	Residue
A	LYS669
A	HOH1254
A	HOH1465

---

site_id	AE4
Number of Residues	5
Details	binding site for residue ACT A 822

Chain	Residue
A	PRO59
A	SER131
A	ASP132
A	HOH1139
A	HOH1332

---

site_id	AE5
Number of Residues	1
Details	binding site for residue ACT A 823

Chain	Residue
A	THR410

---

site_id	AE6
Number of Residues	6
Details	binding site for residue MG A 824

Chain	Residue
A	TYR49
A	ILE67
A	ASN70
A	GLN71
A	TYR72
A	ALA125

---

site_id	AE7
Number of Residues	6
Details	binding site for residue MG A 825

Chain	Residue
A	LYS654
A	ASN655
A	TRP657
A	HOH1263
A	HOH1487
A	HOH1546

---

site_id	AE8
Number of Residues	4
Details	binding site for residue MG A 826

Chain	Residue
A	LYS563
A	HOH1113
A	HOH1453
A	HOH1516

---

site_id	AE9
Number of Residues	5
Details	binding site for residue MG A 827

Chain	Residue
A	ASP169
A	PHE170
A	GLY211
A	HOH956
A	HOH1436

---

site_id	AF1
Number of Residues	4
Details	binding site for residue NI A 828

Chain	Residue
A	HIS585
A	HIS597
A	GLU624
A	GLU628

---

site_id	AF2
Number of Residues	4
Details	binding site for residue NI A 829

Chain	Residue
A	ASN658
A	ASN658
A	GLU659
A	GLU659

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	646
Details	Domain: {"description":"GH81","evidences":[{"source":"PROSITE-ProRule","id":"PRU01352","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	193
Details	Region: {"description":"beta-sandwich subdomain","evidences":[{"source":"PROSITE-ProRule","id":"PRU01352","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	93
Details	Region: {"description":"alpha/beta subdomain","evidences":[{"source":"PROSITE-ProRule","id":"PRU01352","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	349
Details	Region: {"description":"(alpha/beta)6 barrel subdomain","evidences":[{"source":"PROSITE-ProRule","id":"PRU01352","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Active site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01352","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Active site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU01352","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"29870811","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"29870811","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6FOP","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q9KG76","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---