6EVX
Cryo-EM structure of GDP.Pi-microtubule rapidly co-polymerised with doublecortin
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0000226 | biological_process | microtubule cytoskeleton organization |
A | 0000278 | biological_process | mitotic cell cycle |
A | 0003924 | molecular_function | GTPase activity |
A | 0005200 | molecular_function | structural constituent of cytoskeleton |
A | 0005525 | molecular_function | GTP binding |
A | 0005737 | cellular_component | cytoplasm |
A | 0005856 | cellular_component | cytoskeleton |
A | 0005874 | cellular_component | microtubule |
A | 0007017 | biological_process | microtubule-based process |
A | 0015630 | cellular_component | microtubule cytoskeleton |
A | 0016787 | molecular_function | hydrolase activity |
A | 0046872 | molecular_function | metal ion binding |
B | 0000226 | biological_process | microtubule cytoskeleton organization |
B | 0000278 | biological_process | mitotic cell cycle |
B | 0003924 | molecular_function | GTPase activity |
B | 0005200 | molecular_function | structural constituent of cytoskeleton |
B | 0005515 | molecular_function | protein binding |
B | 0005525 | molecular_function | GTP binding |
B | 0005737 | cellular_component | cytoplasm |
B | 0005856 | cellular_component | cytoskeleton |
B | 0005874 | cellular_component | microtubule |
B | 0007017 | biological_process | microtubule-based process |
B | 0046872 | molecular_function | metal ion binding |
C | 0000226 | biological_process | microtubule cytoskeleton organization |
C | 0000278 | biological_process | mitotic cell cycle |
C | 0003924 | molecular_function | GTPase activity |
C | 0005200 | molecular_function | structural constituent of cytoskeleton |
C | 0005525 | molecular_function | GTP binding |
C | 0005737 | cellular_component | cytoplasm |
C | 0005856 | cellular_component | cytoskeleton |
C | 0005874 | cellular_component | microtubule |
C | 0007017 | biological_process | microtubule-based process |
C | 0015630 | cellular_component | microtubule cytoskeleton |
C | 0016787 | molecular_function | hydrolase activity |
C | 0046872 | molecular_function | metal ion binding |
D | 0000226 | biological_process | microtubule cytoskeleton organization |
D | 0000278 | biological_process | mitotic cell cycle |
D | 0003924 | molecular_function | GTPase activity |
D | 0005200 | molecular_function | structural constituent of cytoskeleton |
D | 0005515 | molecular_function | protein binding |
D | 0005525 | molecular_function | GTP binding |
D | 0005737 | cellular_component | cytoplasm |
D | 0005856 | cellular_component | cytoskeleton |
D | 0005874 | cellular_component | microtubule |
D | 0007017 | biological_process | microtubule-based process |
D | 0046872 | molecular_function | metal ion binding |
E | 0000226 | biological_process | microtubule cytoskeleton organization |
E | 0000278 | biological_process | mitotic cell cycle |
E | 0003924 | molecular_function | GTPase activity |
E | 0005200 | molecular_function | structural constituent of cytoskeleton |
E | 0005525 | molecular_function | GTP binding |
E | 0005737 | cellular_component | cytoplasm |
E | 0005856 | cellular_component | cytoskeleton |
E | 0005874 | cellular_component | microtubule |
E | 0007017 | biological_process | microtubule-based process |
E | 0015630 | cellular_component | microtubule cytoskeleton |
E | 0016787 | molecular_function | hydrolase activity |
E | 0046872 | molecular_function | metal ion binding |
F | 0000226 | biological_process | microtubule cytoskeleton organization |
F | 0000278 | biological_process | mitotic cell cycle |
F | 0003924 | molecular_function | GTPase activity |
F | 0005200 | molecular_function | structural constituent of cytoskeleton |
F | 0005515 | molecular_function | protein binding |
F | 0005525 | molecular_function | GTP binding |
F | 0005737 | cellular_component | cytoplasm |
F | 0005856 | cellular_component | cytoskeleton |
F | 0005874 | cellular_component | microtubule |
F | 0007017 | biological_process | microtubule-based process |
F | 0046872 | molecular_function | metal ion binding |
G | 0000226 | biological_process | microtubule cytoskeleton organization |
G | 0000278 | biological_process | mitotic cell cycle |
G | 0003924 | molecular_function | GTPase activity |
G | 0005200 | molecular_function | structural constituent of cytoskeleton |
G | 0005515 | molecular_function | protein binding |
G | 0005525 | molecular_function | GTP binding |
G | 0005737 | cellular_component | cytoplasm |
G | 0005856 | cellular_component | cytoskeleton |
G | 0005874 | cellular_component | microtubule |
G | 0007017 | biological_process | microtubule-based process |
G | 0046872 | molecular_function | metal ion binding |
H | 0000226 | biological_process | microtubule cytoskeleton organization |
H | 0000278 | biological_process | mitotic cell cycle |
H | 0003924 | molecular_function | GTPase activity |
H | 0005200 | molecular_function | structural constituent of cytoskeleton |
H | 0005515 | molecular_function | protein binding |
H | 0005525 | molecular_function | GTP binding |
H | 0005737 | cellular_component | cytoplasm |
H | 0005856 | cellular_component | cytoskeleton |
H | 0005874 | cellular_component | microtubule |
H | 0007017 | biological_process | microtubule-based process |
H | 0046872 | molecular_function | metal ion binding |
I | 0000226 | biological_process | microtubule cytoskeleton organization |
I | 0000278 | biological_process | mitotic cell cycle |
I | 0003924 | molecular_function | GTPase activity |
I | 0005200 | molecular_function | structural constituent of cytoskeleton |
I | 0005515 | molecular_function | protein binding |
I | 0005525 | molecular_function | GTP binding |
I | 0005737 | cellular_component | cytoplasm |
I | 0005856 | cellular_component | cytoskeleton |
I | 0005874 | cellular_component | microtubule |
I | 0007017 | biological_process | microtubule-based process |
I | 0046872 | molecular_function | metal ion binding |
J | 0000226 | biological_process | microtubule cytoskeleton organization |
J | 0000278 | biological_process | mitotic cell cycle |
J | 0003924 | molecular_function | GTPase activity |
J | 0005200 | molecular_function | structural constituent of cytoskeleton |
J | 0005525 | molecular_function | GTP binding |
J | 0005737 | cellular_component | cytoplasm |
J | 0005856 | cellular_component | cytoskeleton |
J | 0005874 | cellular_component | microtubule |
J | 0007017 | biological_process | microtubule-based process |
J | 0015630 | cellular_component | microtubule cytoskeleton |
J | 0016787 | molecular_function | hydrolase activity |
J | 0046872 | molecular_function | metal ion binding |
K | 0000226 | biological_process | microtubule cytoskeleton organization |
K | 0000278 | biological_process | mitotic cell cycle |
K | 0003924 | molecular_function | GTPase activity |
K | 0005200 | molecular_function | structural constituent of cytoskeleton |
K | 0005525 | molecular_function | GTP binding |
K | 0005737 | cellular_component | cytoplasm |
K | 0005856 | cellular_component | cytoskeleton |
K | 0005874 | cellular_component | microtubule |
K | 0007017 | biological_process | microtubule-based process |
K | 0015630 | cellular_component | microtubule cytoskeleton |
K | 0016787 | molecular_function | hydrolase activity |
K | 0046872 | molecular_function | metal ion binding |
L | 0000226 | biological_process | microtubule cytoskeleton organization |
L | 0000278 | biological_process | mitotic cell cycle |
L | 0003924 | molecular_function | GTPase activity |
L | 0005200 | molecular_function | structural constituent of cytoskeleton |
L | 0005525 | molecular_function | GTP binding |
L | 0005737 | cellular_component | cytoplasm |
L | 0005856 | cellular_component | cytoskeleton |
L | 0005874 | cellular_component | microtubule |
L | 0007017 | biological_process | microtubule-based process |
L | 0015630 | cellular_component | microtubule cytoskeleton |
L | 0016787 | molecular_function | hydrolase activity |
L | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 13 |
Details | binding site for residue GDP F 501 |
Chain | Residue |
E | PO4501 |
F | ASP177 |
F | ASN204 |
F | TYR222 |
F | ASN226 |
F | GLY10 |
F | GLN11 |
F | CYS12 |
F | SER138 |
F | GLY141 |
F | GLY142 |
F | THR143 |
F | GLY144 |
site_id | AC2 |
Number of Residues | 18 |
Details | binding site for residue GTP A 501 |
Chain | Residue |
A | GLY10 |
A | GLN11 |
A | ALA12 |
A | GLN15 |
A | GLU71 |
A | ALA99 |
A | ALA100 |
A | ASN101 |
A | SER140 |
A | GLY143 |
A | GLY144 |
A | THR145 |
A | GLY146 |
A | ASN206 |
A | TYR224 |
A | ASN228 |
A | ILE231 |
A | MG502 |
site_id | AC3 |
Number of Residues | 3 |
Details | binding site for residue MG A 502 |
Chain | Residue |
A | GLN11 |
A | GLU71 |
A | GTP501 |
site_id | AC4 |
Number of Residues | 6 |
Details | binding site for residue PO4 A 503 |
Chain | Residue |
A | ASP251 |
A | GLU254 |
B | GLN11 |
B | GLU69 |
B | THR143 |
B | GDP501 |
site_id | AC5 |
Number of Residues | 5 |
Details | binding site for residue PO4 G 501 |
Chain | Residue |
G | GLN11 |
G | GLU69 |
G | ALA97 |
G | THR143 |
G | GDP502 |
site_id | AC6 |
Number of Residues | 13 |
Details | binding site for residue GDP G 502 |
Chain | Residue |
G | GLY10 |
G | GLN11 |
G | CYS12 |
G | SER138 |
G | GLY141 |
G | GLY142 |
G | THR143 |
G | GLY144 |
G | ASP177 |
G | ASN204 |
G | TYR222 |
G | ASN226 |
G | PO4501 |
site_id | AC7 |
Number of Residues | 14 |
Details | binding site for residue GDP D 501 |
Chain | Residue |
C | LEU248 |
C | PO4501 |
D | GLY10 |
D | GLN11 |
D | CYS12 |
D | SER138 |
D | GLY141 |
D | GLY142 |
D | THR143 |
D | GLY144 |
D | ASP177 |
D | ASN204 |
D | TYR222 |
D | ASN226 |
site_id | AC8 |
Number of Residues | 4 |
Details | binding site for residue PO4 I 501 |
Chain | Residue |
I | GLN11 |
I | GLU69 |
I | THR143 |
I | GDP502 |
site_id | AC9 |
Number of Residues | 13 |
Details | binding site for residue GDP I 502 |
Chain | Residue |
I | GLY10 |
I | GLN11 |
I | CYS12 |
I | SER138 |
I | GLY141 |
I | GLY142 |
I | THR143 |
I | GLY144 |
I | ASP177 |
I | ASN204 |
I | TYR222 |
I | ASN226 |
I | PO4501 |
site_id | AD1 |
Number of Residues | 14 |
Details | binding site for residue GDP B 501 |
Chain | Residue |
B | ASP177 |
B | ASN204 |
B | TYR222 |
B | ASN226 |
A | LEU248 |
A | PO4503 |
B | GLY10 |
B | GLN11 |
B | CYS12 |
B | SER138 |
B | GLY141 |
B | GLY142 |
B | THR143 |
B | GLY144 |
site_id | AD2 |
Number of Residues | 4 |
Details | binding site for residue PO4 H 501 |
Chain | Residue |
H | GLN11 |
H | ALA97 |
H | THR143 |
H | GDP502 |
site_id | AD3 |
Number of Residues | 13 |
Details | binding site for residue GDP H 502 |
Chain | Residue |
H | GLY10 |
H | GLN11 |
H | CYS12 |
H | SER138 |
H | GLY141 |
H | GLY142 |
H | THR143 |
H | GLY144 |
H | ASP177 |
H | ASN204 |
H | TYR222 |
H | ASN226 |
H | PO4501 |
site_id | AD4 |
Number of Residues | 6 |
Details | binding site for residue PO4 E 501 |
Chain | Residue |
E | ASP251 |
E | GLU254 |
F | GLN11 |
F | GLU69 |
F | ALA97 |
F | GDP501 |
site_id | AD5 |
Number of Residues | 18 |
Details | binding site for residue GTP E 502 |
Chain | Residue |
E | GLY10 |
E | GLN11 |
E | ALA12 |
E | GLN15 |
E | GLU71 |
E | ALA99 |
E | ALA100 |
E | ASN101 |
E | SER140 |
E | GLY143 |
E | GLY144 |
E | THR145 |
E | GLY146 |
E | ASN206 |
E | TYR224 |
E | ASN228 |
E | ILE231 |
E | MG503 |
site_id | AD6 |
Number of Residues | 3 |
Details | binding site for residue MG E 503 |
Chain | Residue |
E | GLN11 |
E | GLU71 |
E | GTP502 |
site_id | AD7 |
Number of Residues | 18 |
Details | binding site for residue GTP J 501 |
Chain | Residue |
J | GLY10 |
J | GLN11 |
J | ALA12 |
J | GLN15 |
J | GLU71 |
J | ALA99 |
J | ALA100 |
J | ASN101 |
J | SER140 |
J | GLY143 |
J | GLY144 |
J | THR145 |
J | GLY146 |
J | ASN206 |
J | TYR224 |
J | ASN228 |
J | ILE231 |
J | MG502 |
site_id | AD8 |
Number of Residues | 3 |
Details | binding site for residue MG J 502 |
Chain | Residue |
J | GLN11 |
J | GLU71 |
J | GTP501 |
site_id | AD9 |
Number of Residues | 6 |
Details | binding site for residue PO4 C 501 |
Chain | Residue |
C | ASP251 |
C | GLU254 |
D | GLN11 |
D | GLU69 |
D | THR143 |
D | GDP501 |
site_id | AE1 |
Number of Residues | 19 |
Details | binding site for residue GTP C 502 |
Chain | Residue |
C | GLY10 |
C | GLN11 |
C | ALA12 |
C | GLN15 |
C | GLU71 |
C | ALA99 |
C | ALA100 |
C | ASN101 |
C | SER140 |
C | GLY143 |
C | GLY144 |
C | THR145 |
C | GLY146 |
C | ASN206 |
C | TYR224 |
C | ASN228 |
C | ILE231 |
C | MG503 |
I | LYS252 |
site_id | AE2 |
Number of Residues | 3 |
Details | binding site for residue MG C 503 |
Chain | Residue |
C | GLN11 |
C | GLU71 |
C | GTP502 |
site_id | AE3 |
Number of Residues | 18 |
Details | binding site for residue GTP L 501 |
Chain | Residue |
L | GLY10 |
L | GLN11 |
L | ALA12 |
L | GLN15 |
L | GLU71 |
L | ALA99 |
L | ALA100 |
L | ASN101 |
L | SER140 |
L | GLY143 |
L | GLY144 |
L | THR145 |
L | GLY146 |
L | ASN206 |
L | TYR224 |
L | ASN228 |
L | ILE231 |
L | MG502 |
site_id | AE4 |
Number of Residues | 3 |
Details | binding site for residue MG L 502 |
Chain | Residue |
L | GLN11 |
L | GLU71 |
L | GTP501 |
site_id | AE5 |
Number of Residues | 18 |
Details | binding site for residue GTP K 501 |
Chain | Residue |
K | GLY10 |
K | GLN11 |
K | ALA12 |
K | GLN15 |
K | GLU71 |
K | ALA99 |
K | ALA100 |
K | ASN101 |
K | SER140 |
K | GLY143 |
K | GLY144 |
K | THR145 |
K | GLY146 |
K | ASN206 |
K | TYR224 |
K | ASN228 |
K | ILE231 |
K | MG502 |
site_id | AE6 |
Number of Residues | 3 |
Details | binding site for residue MG K 502 |
Chain | Residue |
K | GLN11 |
K | GLU71 |
K | GTP501 |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 6 |
Details | ACT_SITE: ACT_SITE => ECO:0000250|UniProtKB:P68363 |
Chain | Residue | Details |
A | GLU254 | |
G | GLY142 | |
G | THR143 | |
G | GLY144 | |
G | ASN204 | |
G | ASN226 | |
D | GLN11 | |
D | SER138 | |
D | GLY142 | |
D | THR143 | |
D | GLY144 | |
E | GLU254 | |
D | ASN204 | |
D | ASN226 | |
I | GLN11 | |
I | SER138 | |
I | GLY142 | |
I | THR143 | |
I | GLY144 | |
I | ASN204 | |
I | ASN226 | |
B | GLN11 | |
J | GLU254 | |
B | SER138 | |
B | GLY142 | |
B | THR143 | |
B | GLY144 | |
B | ASN204 | |
B | ASN226 | |
H | GLN11 | |
H | SER138 | |
H | GLY142 | |
H | THR143 | |
C | GLU254 | |
H | GLY144 | |
H | ASN204 | |
H | ASN226 | |
L | GLU254 | |
K | GLU254 | |
F | ASN226 | |
G | GLN11 | |
G | SER138 |
site_id | SWS_FT_FI2 |
Number of Residues | 48 |
Details | BINDING: BINDING => ECO:0000250|UniProtKB:P68363 |
Chain | Residue | Details |
A | GLN11 | |
E | GLU71 | |
E | SER140 | |
E | GLY144 | |
E | THR145 | |
E | THR179 | |
E | ASN206 | |
E | ASN228 | |
J | GLN11 | |
J | GLU71 | |
J | SER140 | |
A | GLU71 | |
J | GLY144 | |
J | THR145 | |
J | THR179 | |
J | ASN206 | |
J | ASN228 | |
C | GLN11 | |
C | GLU71 | |
C | SER140 | |
C | GLY144 | |
C | THR145 | |
A | SER140 | |
C | THR179 | |
C | ASN206 | |
C | ASN228 | |
L | GLN11 | |
L | GLU71 | |
L | SER140 | |
L | GLY144 | |
L | THR145 | |
L | THR179 | |
L | ASN206 | |
A | GLY144 | |
L | ASN228 | |
K | GLN11 | |
K | GLU71 | |
K | SER140 | |
K | GLY144 | |
K | THR145 | |
K | THR179 | |
K | ASN206 | |
K | ASN228 | |
A | THR145 | |
A | THR179 | |
A | ASN206 | |
A | ASN228 | |
E | GLN11 |
site_id | SWS_FT_FI3 |
Number of Residues | 6 |
Details | SITE: Involved in polymerization => ECO:0000250 |
Chain | Residue | Details |
A | TYR451 | |
E | TYR451 | |
J | TYR451 | |
C | TYR451 | |
L | TYR451 | |
K | TYR451 |
site_id | SWS_FT_FI4 |
Number of Residues | 6 |
Details | MOD_RES: N6-acetyllysine; alternate => ECO:0000250|UniProtKB:P68363 |
Chain | Residue | Details |
A | LYS40 | |
E | LYS40 | |
J | LYS40 | |
C | LYS40 | |
L | LYS40 | |
K | LYS40 |
site_id | SWS_FT_FI5 |
Number of Residues | 12 |
Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P68363 |
Chain | Residue | Details |
A | SER48 | |
L | SER232 | |
K | SER48 | |
K | SER232 | |
A | SER232 | |
E | SER48 | |
E | SER232 | |
J | SER48 | |
J | SER232 | |
C | SER48 | |
C | SER232 | |
L | SER48 |
site_id | SWS_FT_FI6 |
Number of Residues | 6 |
Details | MOD_RES: 3'-nitrotyrosine => ECO:0000250|UniProtKB:P68373 |
Chain | Residue | Details |
A | TYR282 | |
B | THR290 | |
H | THR285 | |
H | THR290 | |
E | TYR282 | |
J | TYR282 | |
C | TYR282 | |
L | TYR282 | |
K | TYR282 | |
I | THR285 | |
I | THR290 | |
B | THR285 |
site_id | SWS_FT_FI7 |
Number of Residues | 6 |
Details | MOD_RES: Omega-N-methylarginine => ECO:0000250|UniProtKB:P68363 |
Chain | Residue | Details |
A | ARG339 | |
E | ARG339 | |
J | ARG339 | |
C | ARG339 | |
L | ARG339 | |
K | ARG339 |
site_id | SWS_FT_FI8 |
Number of Residues | 6 |
Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P68373 |
Chain | Residue | Details |
A | SER439 | |
E | SER439 | |
J | SER439 | |
C | SER439 | |
L | SER439 | |
K | SER439 |
site_id | SWS_FT_FI9 |
Number of Residues | 6 |
Details | MOD_RES: 5-glutamyl polyglutamate => ECO:0000250|UniProtKB:P68363 |
Chain | Residue | Details |
A | GLU443 | |
E | GLU443 | |
J | GLU443 | |
C | GLU443 | |
L | GLU443 | |
K | GLU443 |
site_id | SWS_FT_FI10 |
Number of Residues | 6 |
Details | MOD_RES: 5-glutamyl polyglutamate => ECO:0000250|UniProtKB:P68369 |
Chain | Residue | Details |
A | GLU445 | |
B | LYS324 | |
H | LYS324 | |
E | GLU445 | |
J | GLU445 | |
C | GLU445 | |
L | GLU445 | |
K | GLU445 | |
I | LYS324 |
site_id | SWS_FT_FI11 |
Number of Residues | 6 |
Details | MOD_RES: 3'-nitrotyrosine => ECO:0000250|UniProtKB:Q71U36 |
Chain | Residue | Details |
A | TYR451 | |
E | TYR451 | |
J | TYR451 | |
C | TYR451 | |
L | TYR451 | |
K | TYR451 |
site_id | SWS_FT_FI12 |
Number of Residues | 18 |
Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250|UniProtKB:P68363 |
Chain | Residue | Details |
A | LYS326 | |
C | LYS326 | |
C | LYS370 | |
L | LYS326 | |
L | LYS370 | |
K | LYS326 | |
K | LYS370 | |
A | LYS370 | |
E | LYS326 | |
E | LYS370 | |
J | LYS326 | |
J | LYS370 |