6E88

Cryo-EM structure of C. elegans GDP-microtubule

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000132	biological_process	establishment of mitotic spindle orientation
A	0000166	molecular_function	nucleotide binding
A	0000226	biological_process	microtubule cytoskeleton organization
A	0000278	biological_process	mitotic cell cycle
A	0005200	molecular_function	structural constituent of cytoskeleton
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005874	cellular_component	microtubule
A	0007017	biological_process	microtubule-based process
A	0008104	biological_process	intracellular protein localization
A	0009792	biological_process	embryo development ending in birth or egg hatching
A	0016787	molecular_function	hydrolase activity
A	0032465	biological_process	regulation of cytokinesis
A	0046872	molecular_function	metal ion binding
A	0051642	biological_process	centrosome localization
B	0000166	molecular_function	nucleotide binding
B	0000212	biological_process	meiotic spindle organization
B	0000226	biological_process	microtubule cytoskeleton organization
B	0000278	biological_process	mitotic cell cycle
B	0003924	molecular_function	GTPase activity
B	0005200	molecular_function	structural constituent of cytoskeleton
B	0005525	molecular_function	GTP binding
B	0005737	cellular_component	cytoplasm
B	0005856	cellular_component	cytoskeleton
B	0005874	cellular_component	microtubule
B	0005876	cellular_component	spindle microtubule
B	0007017	biological_process	microtubule-based process
B	0045298	cellular_component	tubulin complex
B	0046872	molecular_function	metal ion binding
C	0000132	biological_process	establishment of mitotic spindle orientation
C	0000166	molecular_function	nucleotide binding
C	0000226	biological_process	microtubule cytoskeleton organization
C	0000278	biological_process	mitotic cell cycle
C	0005200	molecular_function	structural constituent of cytoskeleton
C	0005525	molecular_function	GTP binding
C	0005737	cellular_component	cytoplasm
C	0005856	cellular_component	cytoskeleton
C	0005874	cellular_component	microtubule
C	0007017	biological_process	microtubule-based process
C	0008104	biological_process	intracellular protein localization
C	0009792	biological_process	embryo development ending in birth or egg hatching
C	0016787	molecular_function	hydrolase activity
C	0032465	biological_process	regulation of cytokinesis
C	0046872	molecular_function	metal ion binding
C	0051642	biological_process	centrosome localization
D	0000166	molecular_function	nucleotide binding
D	0000212	biological_process	meiotic spindle organization
D	0000226	biological_process	microtubule cytoskeleton organization
D	0000278	biological_process	mitotic cell cycle
D	0003924	molecular_function	GTPase activity
D	0005200	molecular_function	structural constituent of cytoskeleton
D	0005525	molecular_function	GTP binding
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0005874	cellular_component	microtubule
D	0005876	cellular_component	spindle microtubule
D	0007017	biological_process	microtubule-based process
D	0045298	cellular_component	tubulin complex
D	0046872	molecular_function	metal ion binding
H	0000132	biological_process	establishment of mitotic spindle orientation
H	0000166	molecular_function	nucleotide binding
H	0000226	biological_process	microtubule cytoskeleton organization
H	0000278	biological_process	mitotic cell cycle
H	0005200	molecular_function	structural constituent of cytoskeleton
H	0005525	molecular_function	GTP binding
H	0005737	cellular_component	cytoplasm
H	0005856	cellular_component	cytoskeleton
H	0005874	cellular_component	microtubule
H	0007017	biological_process	microtubule-based process
H	0008104	biological_process	intracellular protein localization
H	0009792	biological_process	embryo development ending in birth or egg hatching
H	0016787	molecular_function	hydrolase activity
H	0032465	biological_process	regulation of cytokinesis
H	0046872	molecular_function	metal ion binding
H	0051642	biological_process	centrosome localization
I	0000132	biological_process	establishment of mitotic spindle orientation
I	0000166	molecular_function	nucleotide binding
I	0000226	biological_process	microtubule cytoskeleton organization
I	0000278	biological_process	mitotic cell cycle
I	0005200	molecular_function	structural constituent of cytoskeleton
I	0005525	molecular_function	GTP binding
I	0005737	cellular_component	cytoplasm
I	0005856	cellular_component	cytoskeleton
I	0005874	cellular_component	microtubule
I	0007017	biological_process	microtubule-based process
I	0008104	biological_process	intracellular protein localization
I	0009792	biological_process	embryo development ending in birth or egg hatching
I	0016787	molecular_function	hydrolase activity
I	0032465	biological_process	regulation of cytokinesis
I	0046872	molecular_function	metal ion binding
I	0051642	biological_process	centrosome localization
J	0000166	molecular_function	nucleotide binding
J	0000212	biological_process	meiotic spindle organization
J	0000226	biological_process	microtubule cytoskeleton organization
J	0000278	biological_process	mitotic cell cycle
J	0003924	molecular_function	GTPase activity
J	0005200	molecular_function	structural constituent of cytoskeleton
J	0005525	molecular_function	GTP binding
J	0005737	cellular_component	cytoplasm
J	0005856	cellular_component	cytoskeleton
J	0005874	cellular_component	microtubule
J	0005876	cellular_component	spindle microtubule
J	0007017	biological_process	microtubule-based process
J	0045298	cellular_component	tubulin complex
J	0046872	molecular_function	metal ion binding
K	0000166	molecular_function	nucleotide binding
K	0000212	biological_process	meiotic spindle organization
K	0000226	biological_process	microtubule cytoskeleton organization
K	0000278	biological_process	mitotic cell cycle
K	0003924	molecular_function	GTPase activity
K	0005200	molecular_function	structural constituent of cytoskeleton
K	0005525	molecular_function	GTP binding
K	0005737	cellular_component	cytoplasm
K	0005856	cellular_component	cytoskeleton
K	0005874	cellular_component	microtubule
K	0005876	cellular_component	spindle microtubule
K	0007017	biological_process	microtubule-based process
K	0045298	cellular_component	tubulin complex
K	0046872	molecular_function	metal ion binding
L	0000132	biological_process	establishment of mitotic spindle orientation
L	0000166	molecular_function	nucleotide binding
L	0000226	biological_process	microtubule cytoskeleton organization
L	0000278	biological_process	mitotic cell cycle
L	0005200	molecular_function	structural constituent of cytoskeleton
L	0005525	molecular_function	GTP binding
L	0005737	cellular_component	cytoplasm
L	0005856	cellular_component	cytoskeleton
L	0005874	cellular_component	microtubule
L	0007017	biological_process	microtubule-based process
L	0008104	biological_process	intracellular protein localization
L	0009792	biological_process	embryo development ending in birth or egg hatching
L	0016787	molecular_function	hydrolase activity
L	0032465	biological_process	regulation of cytokinesis
L	0046872	molecular_function	metal ion binding
L	0051642	biological_process	centrosome localization
M	0000132	biological_process	establishment of mitotic spindle orientation
M	0000166	molecular_function	nucleotide binding
M	0000226	biological_process	microtubule cytoskeleton organization
M	0000278	biological_process	mitotic cell cycle
M	0005200	molecular_function	structural constituent of cytoskeleton
M	0005525	molecular_function	GTP binding
M	0005737	cellular_component	cytoplasm
M	0005856	cellular_component	cytoskeleton
M	0005874	cellular_component	microtubule
M	0007017	biological_process	microtubule-based process
M	0008104	biological_process	intracellular protein localization
M	0009792	biological_process	embryo development ending in birth or egg hatching
M	0016787	molecular_function	hydrolase activity
M	0032465	biological_process	regulation of cytokinesis
M	0046872	molecular_function	metal ion binding
M	0051642	biological_process	centrosome localization
N	0000166	molecular_function	nucleotide binding
N	0000212	biological_process	meiotic spindle organization
N	0000226	biological_process	microtubule cytoskeleton organization
N	0000278	biological_process	mitotic cell cycle
N	0003924	molecular_function	GTPase activity
N	0005200	molecular_function	structural constituent of cytoskeleton
N	0005525	molecular_function	GTP binding
N	0005737	cellular_component	cytoplasm
N	0005856	cellular_component	cytoskeleton
N	0005874	cellular_component	microtubule
N	0005876	cellular_component	spindle microtubule
N	0007017	biological_process	microtubule-based process
N	0045298	cellular_component	tubulin complex
N	0046872	molecular_function	metal ion binding
O	0000166	molecular_function	nucleotide binding
O	0000212	biological_process	meiotic spindle organization
O	0000226	biological_process	microtubule cytoskeleton organization
O	0000278	biological_process	mitotic cell cycle
O	0003924	molecular_function	GTPase activity
O	0005200	molecular_function	structural constituent of cytoskeleton
O	0005525	molecular_function	GTP binding
O	0005737	cellular_component	cytoplasm
O	0005856	cellular_component	cytoskeleton
O	0005874	cellular_component	microtubule
O	0005876	cellular_component	spindle microtubule
O	0007017	biological_process	microtubule-based process
O	0045298	cellular_component	tubulin complex
O	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	binding site for residue GTP A 501

Chain	Residue
A	GLN11
A	GLN15
A	ASP96
A	ALA98
A	ASN99
A	SER138
A	GLU181
A	TYR222
B	LYS252

---

site_id	AC2
Number of Residues	14
Details	binding site for residue GDP B 501

Chain	Residue
B	GLY10
B	GLN11
B	CYS12
B	GLN15
B	ASN99
B	SER138
B	GLY141
B	GLY142
B	THR143
B	GLY144
B	ASP177
B	ASN204
B	TYR222
B	ASN226

---

site_id	AC3
Number of Residues	16
Details	binding site for residue GTP C 501

Chain	Residue
C	GLY10
C	GLN11
C	ALA12
C	GLN15
C	LEU68
C	ASP96
C	ALA97
C	ALA98
C	ASN99
C	SER138
C	GLY141
C	GLY142
C	THR143
C	GLY144
C	GLU181
C	TYR222

---

site_id	AC4
Number of Residues	13
Details	binding site for residue GDP D 501

Chain	Residue
D	GLN11
D	CYS12
D	GLN15
D	ASN99
D	SER138
D	GLY141
D	GLY142
D	THR143
D	GLY144
D	ASP177
D	ASN204
D	TYR222
D	ASN226

---

site_id	AC5
Number of Residues	11
Details	binding site for residue GTP H 501

Chain	Residue
H	GLN11
H	ALA12
H	GLN15
H	ASP96
H	ASN99
H	SER138
H	GLY141
H	GLY142
H	THR143
H	GLU181
H	TYR222

---

site_id	AC6
Number of Residues	15
Details	binding site for residue GDP J 501

Chain	Residue
J	GLN11
J	CYS12
J	GLN15
J	ASN99
J	SER138
J	GLY141
J	GLY142
J	THR143
J	GLY144
J	ASP177
J	ASN204
J	TYR222
J	LEU225
J	ASN226
L	LEU246

---

site_id	AC7
Number of Residues	14
Details	binding site for residue GTP L 501

Chain	Residue
L	GLY10
L	GLN11
L	ALA12
L	GLN15
L	ASP96
L	ALA97
L	ALA98
L	ASN99
L	SER138
L	GLY141
L	GLY142
L	THR143
L	GLU181
L	TYR222

---

site_id	AC8
Number of Residues	13
Details	binding site for residue GDP N 501

Chain	Residue
N	THR143
N	GLY144
N	ASP177
N	ASN204
N	TYR222
N	ASN226
N	GLN11
N	CYS12
N	GLN15
N	ASN99
N	SER138
N	GLY141
N	GLY142

---

site_id	AC9
Number of Residues	13
Details	binding site for residue GTP I 501

Chain	Residue
I	GLY10
I	GLN11
I	ALA12
I	GLN15
I	ASP96
I	ALA97
I	ALA98
I	ASN99
I	SER138
I	GLY142
I	THR143
I	GLU181
I	TYR222

---

site_id	AD1
Number of Residues	13
Details	binding site for residue GDP K 501

Chain	Residue
K	GLN11
K	CYS12
K	GLN15
K	ASN99
K	SER138
K	GLY141
K	GLY142
K	THR143
K	GLY144
K	ASP177
K	ASN204
K	TYR222
K	ASN226

---

site_id	AD2
Number of Residues	10
Details	binding site for residue GTP M 501

Chain	Residue
M	GLN11
M	GLN15
M	ASP96
M	ALA97
M	ALA98
M	ASN99
M	SER138
M	GLU181
M	TYR222
O	LYS252

---

site_id	AD3
Number of Residues	12
Details	binding site for residue GDP O 501

Chain	Residue
O	GLN11
O	CYS12
O	GLN15
O	ASN99
O	SER138
O	GLY141
O	GLY142
O	THR143
O	GLY144
O	ASP177
O	ASN204
O	ASN226

---

Functional Information from PROSITE/UniProt

site_id	PS00227
Number of Residues	7
Details	TUBULIN Tubulin subunits alpha, beta, and gamma signature. GGGTGSG

Chain	Residue	Details
A	GLY140-GLY146
B	GLY140-GLY146

---

site_id	PS00228
Number of Residues	4
Details	TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MREI

Chain	Residue	Details
B	MET1-ILE4

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	Active site: {"evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	54
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	42
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q13509","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---