Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6E06

Crystal structure of Mycobacterium tuberculosis dethiobiotin synthetase in complex with cytidine triphosphate solved by precipitant-ligand exchange (crystals grown in citrate precipitant)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0004141	molecular_function	dethiobiotin synthase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0009102	biological_process	biotin biosynthetic process
A	0016874	molecular_function	ligase activity
A	0046872	molecular_function	metal ion binding
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0004141	molecular_function	dethiobiotin synthase activity
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0009102	biological_process	biotin biosynthetic process
B	0016874	molecular_function	ligase activity
B	0046872	molecular_function	metal ion binding
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0004141	molecular_function	dethiobiotin synthase activity
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0009102	biological_process	biotin biosynthetic process
C	0016874	molecular_function	ligase activity
C	0046872	molecular_function	metal ion binding
D	0000166	molecular_function	nucleotide binding
D	0000287	molecular_function	magnesium ion binding
D	0004141	molecular_function	dethiobiotin synthase activity
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0009102	biological_process	biotin biosynthetic process
D	0016874	molecular_function	ligase activity
D	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	17
Details	binding site for residue CTP A 301

Chain	Residue
A	THR11
A	GLY169
A	LEU196
A	PRO197
A	GLY199
A	ALA200
A	ALA201
A	MG302
A	HOH401
A	GLY12
A	VAL13
A	GLY14
A	LYS15
A	THR16
A	VAL17
A	ASP49
A	GLU108

site_id	AC2
Number of Residues	5
Details	binding site for residue MG A 302

Chain	Residue
A	THR16
A	ASP49
A	GLU108
A	CTP301
A	HOH401

site_id	AC3
Number of Residues	18
Details	binding site for residue CTP B 301

Chain	Residue
B	GLY12
B	VAL13
B	GLY14
B	LYS15
B	THR16
B	VAL17
B	LYS37
B	ASP49
B	GLU108
B	GLY111
B	GLY169
B	LEU196
B	PRO197
B	GLY199
B	ALA200
B	ALA201
B	MG302
B	HOH412

site_id	AC4
Number of Residues	6
Details	binding site for residue MG B 302

Chain	Residue
B	THR16
B	LYS37
B	ASP49
B	GLU108
B	CTP301
B	HOH412

site_id	AC5
Number of Residues	20
Details	binding site for residue CTP C 301

Chain	Residue
C	THR11
C	GLY12
C	VAL13
C	GLY14
C	LYS15
C	THR16
C	VAL17
C	ASP49
C	GLU108
C	GLY169
C	PRO197
C	GLY199
C	ALA200
C	ALA201
C	MG302
C	HOH407
C	HOH414
C	HOH425
C	HOH443
C	HOH446

site_id	AC6
Number of Residues	6
Details	binding site for residue MG C 302

Chain	Residue
C	THR16
C	LYS37
C	ASP49
C	GLU108
C	CTP301
C	HOH407

site_id	AC7
Number of Residues	17
Details	binding site for residue CTP D 301

Chain	Residue
D	THR11
D	GLY12
D	VAL13
D	GLY14
D	LYS15
D	THR16
D	VAL17
D	ASP49
D	GLU108
D	GLY111
D	GLY169
D	PRO197
D	GLY199
D	ALA200
D	ALA201
D	MG302
D	HOH406

site_id	AC8
Number of Residues	5
Details	binding site for residue MG D 302

Chain	Residue
D	THR16
D	ASP49
D	GLU108
D	CTP301
D	HOH406

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: ACT_SITE => ECO:0000255\|HAMAP-Rule:MF_00336

Chain	Residue	Details
A	LYS37
B	LYS37
C	LYS37
D	LYS37

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269\|PubMed:25801336, ECO:0000269\|PubMed:30289406, ECO:0000269\|DOI:10.1021/acscatal.8b03475, ECO:0007744\|PDB:4WOP, ECO:0007744\|PDB:6CVE, ECO:0007744\|PDB:6CVF, ECO:0007744\|PDB:6E05, ECO:0007744\|PDB:6E06

Chain	Residue	Details
A	THR11
A	PRO197
B	THR11
B	PRO197
C	THR11
C	PRO197
D	THR11
D	PRO197

site_id	SWS_FT_FI3
Number of Residues	12
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00336, ECO:0000269\|PubMed:20565114, ECO:0000269\|PubMed:30289406, ECO:0000269\|DOI:10.1021/acscatal.8b03475, ECO:0007744\|PDB:3FPA, ECO:0007744\|PDB:6CVE, ECO:0007744\|PDB:6CVF, ECO:0007744\|PDB:6CVV, ECO:0007744\|PDB:6CZB, ECO:0007744\|PDB:6CZC, ECO:0007744\|PDB:6CZD, ECO:0007744\|PDB:6CZE, ECO:0007744\|PDB:6E05, ECO:0007744\|PDB:6E06

Chain	Residue	Details
A	THR16
D	THR16
D	ASP49
D	GLU108
A	ASP49
A	GLU108
B	THR16
B	ASP49
B	GLU108
C	THR16
C	ASP49
C	GLU108

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:20565114, ECO:0000269\|DOI:10.1021/acscatal.8b03475, ECO:0007744\|PDB:3FMF, ECO:0007744\|PDB:3FMI, ECO:0007744\|PDB:6CVE

Chain	Residue	Details
A	LYS37
B	LYS37
C	LYS37
D	LYS37

site_id	SWS_FT_FI5
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_00336

Chain	Residue	Details
A	THR41
B	THR41
C	THR41
D	THR41

site_id	SWS_FT_FI6
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:20565114, ECO:0000269\|DOI:10.1021/acscatal.8b03475, ECO:0007744\|PDB:3FMF, ECO:0007744\|PDB:3FMI, ECO:0007744\|PDB:3FPA, ECO:0007744\|PDB:6CVE

Chain	Residue	Details
A	GLY144
B	GLY144
C	GLY144
D	GLY144

site_id	SWS_FT_FI7
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:30289406, ECO:0000269\|DOI:10.1021/acscatal.8b03475, ECO:0007744\|PDB:6CVE, ECO:0007744\|PDB:6CVF, ECO:0007744\|PDB:6CVU, ECO:0007744\|PDB:6E05, ECO:0007744\|PDB:6E06

Chain	Residue	Details
A	GLY169
B	GLY169
C	GLY169
D	GLY169

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)