Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6DVB

Crystal structure of Mycobacterium tuberculosis transcription initiation complex(ECF sigma factor L) containing 5nt RNA with 5nt spacer

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000428	cellular_component	DNA-directed RNA polymerase complex
A	0003677	molecular_function	DNA binding
A	0003899	molecular_function	DNA-directed RNA polymerase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006351	biological_process	DNA-templated transcription
A	0009274	cellular_component	peptidoglycan-based cell wall
A	0016740	molecular_function	transferase activity
A	0016779	molecular_function	nucleotidyltransferase activity
A	0034062	molecular_function	5'-3' RNA polymerase activity
A	0046983	molecular_function	protein dimerization activity
B	0000428	cellular_component	DNA-directed RNA polymerase complex
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed RNA polymerase activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006351	biological_process	DNA-templated transcription
B	0009274	cellular_component	peptidoglycan-based cell wall
B	0016740	molecular_function	transferase activity
B	0016779	molecular_function	nucleotidyltransferase activity
B	0034062	molecular_function	5'-3' RNA polymerase activity
B	0046983	molecular_function	protein dimerization activity
C	0000428	cellular_component	DNA-directed RNA polymerase complex
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed RNA polymerase activity
C	0005515	molecular_function	protein binding
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006351	biological_process	DNA-templated transcription
C	0009274	cellular_component	peptidoglycan-based cell wall
C	0016740	molecular_function	transferase activity
C	0016779	molecular_function	nucleotidyltransferase activity
C	0032549	molecular_function	ribonucleoside binding
C	0034062	molecular_function	5'-3' RNA polymerase activity
C	0046677	biological_process	response to antibiotic
D	0000287	molecular_function	magnesium ion binding
D	0000428	cellular_component	DNA-directed RNA polymerase complex
D	0003677	molecular_function	DNA binding
D	0003899	molecular_function	DNA-directed RNA polymerase activity
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006351	biological_process	DNA-templated transcription
D	0008270	molecular_function	zinc ion binding
D	0009274	cellular_component	peptidoglycan-based cell wall
D	0016740	molecular_function	transferase activity
D	0016779	molecular_function	nucleotidyltransferase activity
D	0034062	molecular_function	5'-3' RNA polymerase activity
D	0046872	molecular_function	metal ion binding
E	0000345	cellular_component	cytosolic DNA-directed RNA polymerase complex
E	0000428	cellular_component	DNA-directed RNA polymerase complex
E	0001000	molecular_function	bacterial-type RNA polymerase core enzyme binding
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed RNA polymerase activity
E	0005829	cellular_component	cytosol
E	0006351	biological_process	DNA-templated transcription
E	0006352	biological_process	DNA-templated transcription initiation
E	0009274	cellular_component	peptidoglycan-based cell wall
E	0016740	molecular_function	transferase activity
E	0016779	molecular_function	nucleotidyltransferase activity
E	0034062	molecular_function	5'-3' RNA polymerase activity
F	0003677	molecular_function	DNA binding
F	0003700	molecular_function	DNA-binding transcription factor activity
F	0005515	molecular_function	protein binding
F	0006352	biological_process	DNA-templated transcription initiation
F	0006355	biological_process	regulation of DNA-templated transcription
F	0016987	molecular_function	sigma factor activity
F	2000142	biological_process	regulation of DNA-templated transcription initiation

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN D 2001

Chain	Residue
D	CYS891
D	CYS968
D	CYS975
D	CYS978

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN D 2002

Chain	Residue
D	CYS60
D	CYS62
D	CYS75
D	CYS78

Functional Information from PROSITE/UniProt

site_id	PS01063
Number of Residues	33
Details	SIGMA70_ECF Sigma-70 factors ECF subfamily signature. AEDVVQeTLLrAWqhpevigdtarparAWLftV

Chain	Residue	Details
F	ALA40-VAL72

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKLAGrHGNKGV

Chain	Residue	Details
C	GLY882-VAL894

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	225
Details	Region: {"description":"Alpha N-terminal domain (alpha-NTD)","evidences":[{"source":"HAMAP-Rule","id":"MF_00059","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	11
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01322","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	19
Details	DNA binding: {"description":"H-T-H motif","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	67
Details	Region: {"description":"Sigma-70 factor domain-2"}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	48
Details	Region: {"description":"Sigma-70 factor domain-4"}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	3
Details	Motif: {"description":"Interaction with polymerase core subunit RpoC","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)