6CZF

The structure of E. coli PurF in complex with ppGpp-Mg

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004044	molecular_function	amidophosphoribosyltransferase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006164	biological_process	purine nucleotide biosynthetic process
A	0006189	biological_process	'de novo' IMP biosynthetic process
A	0006541	biological_process	glutamine metabolic process
A	0009113	biological_process	purine nucleobase biosynthetic process
A	0016757	molecular_function	glycosyltransferase activity
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
A	0097216	molecular_function	guanosine tetraphosphate binding
B	0000287	molecular_function	magnesium ion binding
B	0004044	molecular_function	amidophosphoribosyltransferase activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006164	biological_process	purine nucleotide biosynthetic process
B	0006189	biological_process	'de novo' IMP biosynthetic process
B	0006541	biological_process	glutamine metabolic process
B	0009113	biological_process	purine nucleobase biosynthetic process
B	0016757	molecular_function	glycosyltransferase activity
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding
B	0097216	molecular_function	guanosine tetraphosphate binding
C	0000287	molecular_function	magnesium ion binding
C	0004044	molecular_function	amidophosphoribosyltransferase activity
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006164	biological_process	purine nucleotide biosynthetic process
C	0006189	biological_process	'de novo' IMP biosynthetic process
C	0006541	biological_process	glutamine metabolic process
C	0009113	biological_process	purine nucleobase biosynthetic process
C	0016757	molecular_function	glycosyltransferase activity
C	0042802	molecular_function	identical protein binding
C	0046872	molecular_function	metal ion binding
C	0097216	molecular_function	guanosine tetraphosphate binding
D	0000287	molecular_function	magnesium ion binding
D	0004044	molecular_function	amidophosphoribosyltransferase activity
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006164	biological_process	purine nucleotide biosynthetic process
D	0006189	biological_process	'de novo' IMP biosynthetic process
D	0006541	biological_process	glutamine metabolic process
D	0009113	biological_process	purine nucleobase biosynthetic process
D	0016757	molecular_function	glycosyltransferase activity
D	0042802	molecular_function	identical protein binding
D	0046872	molecular_function	metal ion binding
D	0097216	molecular_function	guanosine tetraphosphate binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	1
Details	binding site for residue MG A 501

Chain	Residue
A	G4P502

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site_id	AC2
Number of Residues	21
Details	binding site for residue G4P A 502

Chain	Residue
A	SER83
A	GLU84
A	MG501
A	HOH655
C	ARG45
C	LYS46
C	ASN48
C	ARG58
C	HIS59
C	ARG62
A	ARG45
C	SER81
C	ALA82
C	SER83
A	LYS46
A	ASN48
A	ARG58
A	HIS59
A	ARG62
A	SER81
A	ALA82

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site_id	AC3
Number of Residues	1
Details	binding site for residue MG D 501

Chain	Residue
D	G4P502

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site_id	AC4
Number of Residues	21
Details	binding site for residue G4P D 502

Chain	Residue
B	ARG45
B	LYS46
B	ASN48
B	ARG58
B	HIS59
B	ARG62
B	SER81
B	ALA82
B	SER83
B	HOH688
D	ARG45
D	LYS46
D	ASN48
D	ARG58
D	HIS59
D	ARG62
D	SER81
D	ALA82
D	SER83
D	MG501
D	HOH658

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Functional Information from PROSITE/UniProt

site_id	PS00103
Number of Residues	13
Details	PUR_PYR_PR_TRANSFER Purine/pyrimidine phosphoribosyl transferases signature. VLLVDDSIVRGtT

Chain	Residue	Details
A	VAL362-THR374

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Nucleophile => ECO:0000255|HAMAP-Rule:MF_01931, ECO:0000269|PubMed:7037784

Chain	Residue	Details
A	CYS1
B	CYS1
C	CYS1
D	CYS1

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site_id	SWS_FT_FI2
Number of Residues	12
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01931

Chain	Residue	Details
A	THR304
D	THR304
D	ASP366
D	ASP367
A	ASP366
A	ASP367
B	THR304
B	ASP366
B	ASP367
C	THR304
C	ASP366
C	ASP367

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 214

Chain	Residue	Details
A	CYS1	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
A	GLY27	activator, electrostatic stabiliser, hydrogen bond acceptor
A	ASN101	electrostatic stabiliser, hydrogen bond donor
A	GLY102	electrostatic stabiliser, hydrogen bond donor
A	TYR258	electrostatic stabiliser, hydrogen bond donor

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site_id	MCSA2
Number of Residues	5
Details	M-CSA 214

Chain	Residue	Details
B	CYS1	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
B	GLY27	activator, electrostatic stabiliser, hydrogen bond acceptor
B	ASN101	electrostatic stabiliser, hydrogen bond donor
B	GLY102	electrostatic stabiliser, hydrogen bond donor
B	TYR258	electrostatic stabiliser, hydrogen bond donor

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site_id	MCSA3
Number of Residues	5
Details	M-CSA 214

Chain	Residue	Details
C	CYS1	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
C	GLY27	activator, electrostatic stabiliser, hydrogen bond acceptor
C	ASN101	electrostatic stabiliser, hydrogen bond donor
C	GLY102	electrostatic stabiliser, hydrogen bond donor
C	TYR258	electrostatic stabiliser, hydrogen bond donor

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site_id	MCSA4
Number of Residues	5
Details	M-CSA 214

Chain	Residue	Details
D	CYS1	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
D	GLY27	activator, electrostatic stabiliser, hydrogen bond acceptor
D	ASN101	electrostatic stabiliser, hydrogen bond donor
D	GLY102	electrostatic stabiliser, hydrogen bond donor
D	TYR258	electrostatic stabiliser, hydrogen bond donor

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