6CZF
The structure of E. coli PurF in complex with ppGpp-Mg
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0000287 | molecular_function | magnesium ion binding |
A | 0004044 | molecular_function | amidophosphoribosyltransferase activity |
A | 0005737 | cellular_component | cytoplasm |
A | 0005829 | cellular_component | cytosol |
A | 0006164 | biological_process | purine nucleotide biosynthetic process |
A | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
A | 0006541 | biological_process | glutamine metabolic process |
A | 0009113 | biological_process | purine nucleobase biosynthetic process |
A | 0016757 | molecular_function | glycosyltransferase activity |
A | 0042802 | molecular_function | identical protein binding |
A | 0046872 | molecular_function | metal ion binding |
A | 0097216 | molecular_function | guanosine tetraphosphate binding |
B | 0000287 | molecular_function | magnesium ion binding |
B | 0004044 | molecular_function | amidophosphoribosyltransferase activity |
B | 0005737 | cellular_component | cytoplasm |
B | 0005829 | cellular_component | cytosol |
B | 0006164 | biological_process | purine nucleotide biosynthetic process |
B | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
B | 0006541 | biological_process | glutamine metabolic process |
B | 0009113 | biological_process | purine nucleobase biosynthetic process |
B | 0016757 | molecular_function | glycosyltransferase activity |
B | 0042802 | molecular_function | identical protein binding |
B | 0046872 | molecular_function | metal ion binding |
B | 0097216 | molecular_function | guanosine tetraphosphate binding |
C | 0000287 | molecular_function | magnesium ion binding |
C | 0004044 | molecular_function | amidophosphoribosyltransferase activity |
C | 0005737 | cellular_component | cytoplasm |
C | 0005829 | cellular_component | cytosol |
C | 0006164 | biological_process | purine nucleotide biosynthetic process |
C | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
C | 0006541 | biological_process | glutamine metabolic process |
C | 0009113 | biological_process | purine nucleobase biosynthetic process |
C | 0016757 | molecular_function | glycosyltransferase activity |
C | 0042802 | molecular_function | identical protein binding |
C | 0046872 | molecular_function | metal ion binding |
C | 0097216 | molecular_function | guanosine tetraphosphate binding |
D | 0000287 | molecular_function | magnesium ion binding |
D | 0004044 | molecular_function | amidophosphoribosyltransferase activity |
D | 0005737 | cellular_component | cytoplasm |
D | 0005829 | cellular_component | cytosol |
D | 0006164 | biological_process | purine nucleotide biosynthetic process |
D | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
D | 0006541 | biological_process | glutamine metabolic process |
D | 0009113 | biological_process | purine nucleobase biosynthetic process |
D | 0016757 | molecular_function | glycosyltransferase activity |
D | 0042802 | molecular_function | identical protein binding |
D | 0046872 | molecular_function | metal ion binding |
D | 0097216 | molecular_function | guanosine tetraphosphate binding |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 1 |
Details | binding site for residue MG A 501 |
Chain | Residue |
A | G4P502 |
site_id | AC2 |
Number of Residues | 21 |
Details | binding site for residue G4P A 502 |
Chain | Residue |
A | SER83 |
A | GLU84 |
A | MG501 |
A | HOH655 |
C | ARG45 |
C | LYS46 |
C | ASN48 |
C | ARG58 |
C | HIS59 |
C | ARG62 |
A | ARG45 |
C | SER81 |
C | ALA82 |
C | SER83 |
A | LYS46 |
A | ASN48 |
A | ARG58 |
A | HIS59 |
A | ARG62 |
A | SER81 |
A | ALA82 |
site_id | AC3 |
Number of Residues | 1 |
Details | binding site for residue MG D 501 |
Chain | Residue |
D | G4P502 |
site_id | AC4 |
Number of Residues | 21 |
Details | binding site for residue G4P D 502 |
Chain | Residue |
B | ARG45 |
B | LYS46 |
B | ASN48 |
B | ARG58 |
B | HIS59 |
B | ARG62 |
B | SER81 |
B | ALA82 |
B | SER83 |
B | HOH688 |
D | ARG45 |
D | LYS46 |
D | ASN48 |
D | ARG58 |
D | HIS59 |
D | ARG62 |
D | SER81 |
D | ALA82 |
D | SER83 |
D | MG501 |
D | HOH658 |
Functional Information from PROSITE/UniProt
site_id | PS00103 |
Number of Residues | 13 |
Details | PUR_PYR_PR_TRANSFER Purine/pyrimidine phosphoribosyl transferases signature. VLLVDDSIVRGtT |
Chain | Residue | Details |
A | VAL362-THR374 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 4 |
Details | ACT_SITE: Nucleophile => ECO:0000255|HAMAP-Rule:MF_01931, ECO:0000269|PubMed:7037784 |
Chain | Residue | Details |
A | CYS1 | |
B | CYS1 | |
C | CYS1 | |
D | CYS1 |
site_id | SWS_FT_FI2 |
Number of Residues | 12 |
Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01931 |
Chain | Residue | Details |
A | THR304 | |
D | THR304 | |
D | ASP366 | |
D | ASP367 | |
A | ASP366 | |
A | ASP367 | |
B | THR304 | |
B | ASP366 | |
B | ASP367 | |
C | THR304 | |
C | ASP366 | |
C | ASP367 |
Catalytic Information from CSA
site_id | MCSA1 |
Number of Residues | 5 |
Details | M-CSA 214 |
Chain | Residue | Details |
A | CYS1 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
A | GLY27 | activator, electrostatic stabiliser, hydrogen bond acceptor |
A | ASN101 | electrostatic stabiliser, hydrogen bond donor |
A | GLY102 | electrostatic stabiliser, hydrogen bond donor |
A | TYR258 | electrostatic stabiliser, hydrogen bond donor |
site_id | MCSA2 |
Number of Residues | 5 |
Details | M-CSA 214 |
Chain | Residue | Details |
B | CYS1 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
B | GLY27 | activator, electrostatic stabiliser, hydrogen bond acceptor |
B | ASN101 | electrostatic stabiliser, hydrogen bond donor |
B | GLY102 | electrostatic stabiliser, hydrogen bond donor |
B | TYR258 | electrostatic stabiliser, hydrogen bond donor |
site_id | MCSA3 |
Number of Residues | 5 |
Details | M-CSA 214 |
Chain | Residue | Details |
C | CYS1 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
C | GLY27 | activator, electrostatic stabiliser, hydrogen bond acceptor |
C | ASN101 | electrostatic stabiliser, hydrogen bond donor |
C | GLY102 | electrostatic stabiliser, hydrogen bond donor |
C | TYR258 | electrostatic stabiliser, hydrogen bond donor |
site_id | MCSA4 |
Number of Residues | 5 |
Details | M-CSA 214 |
Chain | Residue | Details |
D | CYS1 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
D | GLY27 | activator, electrostatic stabiliser, hydrogen bond acceptor |
D | ASN101 | electrostatic stabiliser, hydrogen bond donor |
D | GLY102 | electrostatic stabiliser, hydrogen bond donor |
D | TYR258 | electrostatic stabiliser, hydrogen bond donor |