6CZF
The structure of E. coli PurF in complex with ppGpp-Mg
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0004044 | molecular_function | amidophosphoribosyltransferase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006164 | biological_process | purine nucleotide biosynthetic process |
| A | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
| A | 0006541 | biological_process | glutamine metabolic process |
| A | 0009113 | biological_process | purine nucleobase biosynthetic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016757 | molecular_function | glycosyltransferase activity |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0097216 | molecular_function | guanosine tetraphosphate binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0004044 | molecular_function | amidophosphoribosyltransferase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006164 | biological_process | purine nucleotide biosynthetic process |
| B | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
| B | 0006541 | biological_process | glutamine metabolic process |
| B | 0009113 | biological_process | purine nucleobase biosynthetic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016757 | molecular_function | glycosyltransferase activity |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0097216 | molecular_function | guanosine tetraphosphate binding |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0004044 | molecular_function | amidophosphoribosyltransferase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006164 | biological_process | purine nucleotide biosynthetic process |
| C | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
| C | 0006541 | biological_process | glutamine metabolic process |
| C | 0009113 | biological_process | purine nucleobase biosynthetic process |
| C | 0016740 | molecular_function | transferase activity |
| C | 0016757 | molecular_function | glycosyltransferase activity |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0097216 | molecular_function | guanosine tetraphosphate binding |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0004044 | molecular_function | amidophosphoribosyltransferase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006164 | biological_process | purine nucleotide biosynthetic process |
| D | 0006189 | biological_process | 'de novo' IMP biosynthetic process |
| D | 0006541 | biological_process | glutamine metabolic process |
| D | 0009113 | biological_process | purine nucleobase biosynthetic process |
| D | 0016740 | molecular_function | transferase activity |
| D | 0016757 | molecular_function | glycosyltransferase activity |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0097216 | molecular_function | guanosine tetraphosphate binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 1 |
| Details | binding site for residue MG A 501 |
| Chain | Residue |
| A | G4P502 |
| site_id | AC2 |
| Number of Residues | 21 |
| Details | binding site for residue G4P A 502 |
| Chain | Residue |
| A | SER83 |
| A | GLU84 |
| A | MG501 |
| A | HOH655 |
| C | ARG45 |
| C | LYS46 |
| C | ASN48 |
| C | ARG58 |
| C | HIS59 |
| C | ARG62 |
| A | ARG45 |
| C | SER81 |
| C | ALA82 |
| C | SER83 |
| A | LYS46 |
| A | ASN48 |
| A | ARG58 |
| A | HIS59 |
| A | ARG62 |
| A | SER81 |
| A | ALA82 |
| site_id | AC3 |
| Number of Residues | 1 |
| Details | binding site for residue MG D 501 |
| Chain | Residue |
| D | G4P502 |
| site_id | AC4 |
| Number of Residues | 21 |
| Details | binding site for residue G4P D 502 |
| Chain | Residue |
| B | ARG45 |
| B | LYS46 |
| B | ASN48 |
| B | ARG58 |
| B | HIS59 |
| B | ARG62 |
| B | SER81 |
| B | ALA82 |
| B | SER83 |
| B | HOH688 |
| D | ARG45 |
| D | LYS46 |
| D | ASN48 |
| D | ARG58 |
| D | HIS59 |
| D | ARG62 |
| D | SER81 |
| D | ALA82 |
| D | SER83 |
| D | MG501 |
| D | HOH658 |
Functional Information from PROSITE/UniProt
| site_id | PS00103 |
| Number of Residues | 13 |
| Details | PUR_PYR_PR_TRANSFER Purine/pyrimidine phosphoribosyl transferases signature. VLLVDDSIVRGtT |
| Chain | Residue | Details |
| A | VAL362-THR374 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 936 |
| Details | Domain: {"description":"Glutamine amidotransferase type-2","evidences":[{"source":"HAMAP-Rule","id":"MF_01931","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"HAMAP-Rule","id":"MF_01931","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"7037784","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 12 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01931","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 214 |
| Chain | Residue | Details |
| A | CYS1 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| A | GLY27 | activator, electrostatic stabiliser, hydrogen bond acceptor |
| A | ASN101 | electrostatic stabiliser, hydrogen bond donor |
| A | GLY102 | electrostatic stabiliser, hydrogen bond donor |
| A | TYR258 | electrostatic stabiliser, hydrogen bond donor |
| site_id | MCSA2 |
| Number of Residues | 5 |
| Details | M-CSA 214 |
| Chain | Residue | Details |
| B | CYS1 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| B | GLY27 | activator, electrostatic stabiliser, hydrogen bond acceptor |
| B | ASN101 | electrostatic stabiliser, hydrogen bond donor |
| B | GLY102 | electrostatic stabiliser, hydrogen bond donor |
| B | TYR258 | electrostatic stabiliser, hydrogen bond donor |
| site_id | MCSA3 |
| Number of Residues | 5 |
| Details | M-CSA 214 |
| Chain | Residue | Details |
| C | CYS1 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| C | GLY27 | activator, electrostatic stabiliser, hydrogen bond acceptor |
| C | ASN101 | electrostatic stabiliser, hydrogen bond donor |
| C | GLY102 | electrostatic stabiliser, hydrogen bond donor |
| C | TYR258 | electrostatic stabiliser, hydrogen bond donor |
| site_id | MCSA4 |
| Number of Residues | 5 |
| Details | M-CSA 214 |
| Chain | Residue | Details |
| D | CYS1 | covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor |
| D | GLY27 | activator, electrostatic stabiliser, hydrogen bond acceptor |
| D | ASN101 | electrostatic stabiliser, hydrogen bond donor |
| D | GLY102 | electrostatic stabiliser, hydrogen bond donor |
| D | TYR258 | electrostatic stabiliser, hydrogen bond donor |
