6CXK

E. coli DHFR substrate complex with Dihydrofolate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004146	molecular_function	dihydrofolate reductase activity
A	0005515	molecular_function	protein binding
A	0005542	molecular_function	folic acid binding
A	0005829	cellular_component	cytosol
A	0006730	biological_process	one-carbon metabolic process
A	0009257	biological_process	10-formyltetrahydrofolate biosynthetic process
A	0009410	biological_process	response to xenobiotic stimulus
A	0016491	molecular_function	oxidoreductase activity
A	0031427	biological_process	response to methotrexate
A	0044281	biological_process	small molecule metabolic process
A	0046452	biological_process	dihydrofolate metabolic process
A	0046654	biological_process	tetrahydrofolate biosynthetic process
A	0046655	biological_process	folic acid metabolic process
A	0046656	biological_process	folic acid biosynthetic process
A	0046677	biological_process	response to antibiotic
A	0050661	molecular_function	NADP binding
A	0051870	molecular_function	methotrexate binding
A	0051871	molecular_function	dihydrofolic acid binding
A	0070401	molecular_function	NADP+ binding
A	0070402	molecular_function	NADPH binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue MG A 201

Chain	Residue
A	GLU101
A	GLU139
A	HOH340
A	HOH373
A	HOH384
A	HOH420

---

site_id	AC2
Number of Residues	5
Details	binding site for residue MG A 202

Chain	Residue
A	HOH448
A	HOH461
A	GLU101
A	SER138
A	HOH386

---

site_id	AC3
Number of Residues	4
Details	binding site for residue MG A 203

Chain	Residue
A	HIS124
A	HOH326
A	HOH342
A	HOH467

---

site_id	AC4
Number of Residues	6
Details	binding site for residue MG A 204

Chain	Residue
A	HIS160
A	HOH328
A	HOH378
A	HOH412
A	HOH429
A	HOH471

---

site_id	AC5
Number of Residues	5
Details	binding site for residue MG A 205

Chain	Residue
A	ASP142
A	HIS163
A	HOH312
A	HOH354
A	HOH409

---

site_id	AC6
Number of Residues	6
Details	binding site for residue MG A 206

Chain	Residue
A	ASN18
A	ASP70
A	HOH305
A	HOH319
A	HOH324
A	HOH462

---

site_id	AC7
Number of Residues	6
Details	binding site for residue MG A 207

Chain	Residue
A	ASP87
A	HOH318
A	HOH331
A	HOH419
A	HOH426
A	HOH435

---

site_id	AC8
Number of Residues	7
Details	binding site for residue MG A 208

Chain	Residue
A	GLU120
A	HOH316
A	HOH346
A	HOH358
A	HOH381
A	HOH463
A	HOH470

---

site_id	AC9
Number of Residues	5
Details	binding site for residue CL A 209

Chain	Residue
A	GLY43
A	HIS45
A	THR46
A	GLY96
A	HOH452

---

site_id	AD1
Number of Residues	17
Details	binding site for residue DHF A 210

Chain	Residue
A	ILE5
A	ALA6
A	ALA7
A	MET16
A	MET20
A	ASP27
A	PHE31
A	LYS32
A	ARG57
A	ILE94
A	TYR100
A	THR113
A	HOH306
A	HOH308
A	HOH309
A	HOH366
A	HOH388

---

Functional Information from PROSITE/UniProt

site_id	PS00075
Number of Residues	23
Details	DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGmenaMPWnlpa.DlawFkrnT

Chain	Residue	Details
A	VAL13-THR35

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	157
Details	Domain: {"description":"DHFR","evidences":[{"source":"PROSITE-ProRule","id":"PRU00660","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	22
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---