6COE
AtHNL enantioselectivity mutant At-A9-H7 Apo Y13C,Y121L,P126F,L128W,C131T,F179L,A209I with benzaldehyde, MANDELIC ACID NITRILE
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0002213 | biological_process | defense response to insect |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0009611 | biological_process | response to wounding |
| A | 0009694 | biological_process | jasmonic acid metabolic process |
| A | 0009696 | biological_process | salicylic acid metabolic process |
| A | 0016139 | biological_process | glycoside catabolic process |
| A | 0016829 | molecular_function | lyase activity |
| A | 0046593 | molecular_function | mandelonitrile lyase activity |
| A | 0080030 | molecular_function | methyl indole-3-acetate esterase activity |
| A | 0080031 | molecular_function | methyl salicylate esterase activity |
| A | 0080032 | molecular_function | methyl jasmonate esterase activity |
| B | 0002213 | biological_process | defense response to insect |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0009611 | biological_process | response to wounding |
| B | 0009694 | biological_process | jasmonic acid metabolic process |
| B | 0009696 | biological_process | salicylic acid metabolic process |
| B | 0016139 | biological_process | glycoside catabolic process |
| B | 0016829 | molecular_function | lyase activity |
| B | 0046593 | molecular_function | mandelonitrile lyase activity |
| B | 0080030 | molecular_function | methyl indole-3-acetate esterase activity |
| B | 0080031 | molecular_function | methyl salicylate esterase activity |
| B | 0080032 | molecular_function | methyl jasmonate esterase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 301 |
| Chain | Residue |
| A | GLU53 |
| A | ASN141 |
| A | MET144 |
| A | ASP183 |
| A | LYS186 |
| A | HOH432 |
| A | HOH467 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | binding site for residue GOL A 302 |
| Chain | Residue |
| A | HIS137 |
| A | GLU138 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | binding site for residue GOL A 303 |
| Chain | Residue |
| A | GLY128 |
| A | GLY130 |
| A | PHE153 |
| A | ARG157 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue CL A 304 |
| Chain | Residue |
| A | ASN12 |
| A | ALA13 |
| A | SER81 |
| A | PHE82 |
| A | MNN306 |
| A | MXN307 |
| site_id | AC5 |
| Number of Residues | 1 |
| Details | binding site for residue HBX A 305 |
| Chain | Residue |
| A | HOH408 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | binding site for residue GOL B 301 |
| Chain | Residue |
| B | GLU53 |
| B | THR139 |
| B | ARG140 |
| B | ASN161 |
| B | GLY233 |
| B | LYS242 |
| B | HOH401 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | binding site for residue CL B 302 |
| Chain | Residue |
| B | ASN12 |
| B | ALA13 |
| B | SER81 |
| B | PHE82 |
| B | MNN304 |
| B | MXN305 |
| site_id | AC8 |
| Number of Residues | 11 |
| Details | binding site for residues MNN A 306 and MXN A 307 |
| Chain | Residue |
| A | ASN12 |
| A | ALA13 |
| A | SER81 |
| A | PHE82 |
| A | PHE107 |
| A | LEU122 |
| A | TRP129 |
| A | MET149 |
| A | ILE210 |
| A | HIS236 |
| A | CL304 |
| site_id | AC9 |
| Number of Residues | 11 |
| Details | binding site for residues MNN A 306 and MXN A 307 |
| Chain | Residue |
| A | ASN12 |
| A | ALA13 |
| A | SER81 |
| A | PHE82 |
| A | PHE107 |
| A | LEU122 |
| A | TRP129 |
| A | MET149 |
| A | ILE210 |
| A | HIS236 |
| A | CL304 |
| site_id | AD1 |
| Number of Residues | 11 |
| Details | binding site for residues MNN A 306 and MXN A 307 |
| Chain | Residue |
| A | ASN12 |
| A | ALA13 |
| A | SER81 |
| A | PHE82 |
| A | PHE107 |
| A | LEU122 |
| A | TRP129 |
| A | MET149 |
| A | ILE210 |
| A | HIS236 |
| A | CL304 |
| site_id | AD2 |
| Number of Residues | 11 |
| Details | binding site for residues MNN A 306 and MXN A 307 |
| Chain | Residue |
| A | ASN12 |
| A | ALA13 |
| A | SER81 |
| A | PHE82 |
| A | PHE107 |
| A | LEU122 |
| A | TRP129 |
| A | MET149 |
| A | ILE210 |
| A | HIS236 |
| A | CL304 |
| site_id | AD3 |
| Number of Residues | 11 |
| Details | binding site for residues MNN A 306 and MXN A 307 |
| Chain | Residue |
| A | ASN12 |
| A | ALA13 |
| A | SER81 |
| A | PHE82 |
| A | PHE107 |
| A | LEU122 |
| A | TRP129 |
| A | MET149 |
| A | ILE210 |
| A | HIS236 |
| A | CL304 |
| site_id | AD4 |
| Number of Residues | 11 |
| Details | binding site for residues MNN A 306 and MXN A 307 |
| Chain | Residue |
| A | ASN12 |
| A | ALA13 |
| A | SER81 |
| A | PHE82 |
| A | PHE107 |
| A | LEU122 |
| A | TRP129 |
| A | MET149 |
| A | ILE210 |
| A | HIS236 |
| A | CL304 |
| site_id | AD5 |
| Number of Residues | 11 |
| Details | binding site for residues MNN A 306 and MXN A 307 |
| Chain | Residue |
| A | ASN12 |
| A | ALA13 |
| A | SER81 |
| A | PHE82 |
| A | PHE107 |
| A | LEU122 |
| A | TRP129 |
| A | MET149 |
| A | ILE210 |
| A | HIS236 |
| A | CL304 |
| site_id | AD6 |
| Number of Residues | 11 |
| Details | binding site for residues MNN A 306 and MXN A 307 |
| Chain | Residue |
| A | ASN12 |
| A | ALA13 |
| A | SER81 |
| A | PHE82 |
| A | PHE107 |
| A | LEU122 |
| A | TRP129 |
| A | MET149 |
| A | ILE210 |
| A | HIS236 |
| A | CL304 |
| site_id | AD7 |
| Number of Residues | 11 |
| Details | binding site for residues MNN B 304 and MXN B 305 |
| Chain | Residue |
| B | ASN12 |
| B | ALA13 |
| B | SER81 |
| B | PHE82 |
| B | PHE107 |
| B | MET123 |
| B | PHE126 |
| B | TRP129 |
| B | MET149 |
| B | HIS236 |
| B | CL302 |
| site_id | AD8 |
| Number of Residues | 11 |
| Details | binding site for residues MNN B 304 and MXN B 305 |
| Chain | Residue |
| B | ASN12 |
| B | ALA13 |
| B | SER81 |
| B | PHE82 |
| B | PHE107 |
| B | MET123 |
| B | PHE126 |
| B | TRP129 |
| B | MET149 |
| B | HIS236 |
| B | CL302 |
| site_id | AD9 |
| Number of Residues | 11 |
| Details | binding site for residues MNN B 304 and MXN B 305 |
| Chain | Residue |
| B | ASN12 |
| B | ALA13 |
| B | SER81 |
| B | PHE82 |
| B | PHE107 |
| B | MET123 |
| B | PHE126 |
| B | TRP129 |
| B | MET149 |
| B | HIS236 |
| B | CL302 |
| site_id | AE1 |
| Number of Residues | 11 |
| Details | binding site for residues MNN B 304 and MXN B 305 |
| Chain | Residue |
| B | ASN12 |
| B | ALA13 |
| B | SER81 |
| B | PHE82 |
| B | PHE107 |
| B | MET123 |
| B | PHE126 |
| B | TRP129 |
| B | MET149 |
| B | HIS236 |
| B | CL302 |
| site_id | AE2 |
| Number of Residues | 11 |
| Details | binding site for residues MNN B 304 and MXN B 305 |
| Chain | Residue |
| B | ASN12 |
| B | ALA13 |
| B | SER81 |
| B | PHE82 |
| B | PHE107 |
| B | MET123 |
| B | PHE126 |
| B | TRP129 |
| B | MET149 |
| B | HIS236 |
| B | CL302 |
| site_id | AE3 |
| Number of Residues | 11 |
| Details | binding site for residues MNN B 304 and MXN B 305 |
| Chain | Residue |
| B | ASN12 |
| B | ALA13 |
| B | SER81 |
| B | PHE82 |
| B | PHE107 |
| B | MET123 |
| B | PHE126 |
| B | TRP129 |
| B | MET149 |
| B | HIS236 |
| B | CL302 |
| site_id | AE4 |
| Number of Residues | 11 |
| Details | binding site for residues MNN B 304 and MXN B 305 |
| Chain | Residue |
| B | ASN12 |
| B | ALA13 |
| B | SER81 |
| B | PHE82 |
| B | PHE107 |
| B | MET123 |
| B | PHE126 |
| B | TRP129 |
| B | MET149 |
| B | HIS236 |
| B | CL302 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000250|UniProtKB:P52704 |
| Chain | Residue | Details |
| A | SER81 | |
| A | HIS236 | |
| B | SER81 | |
| B | HIS236 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | SITE: Increases basicity of active site His => ECO:0000250|UniProtKB:P52704 |
| Chain | Residue | Details |
| A | ASP208 | |
| B | ASP208 |
