6CN1
2.75 Angstrom Resolution Crystal Structure of UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Pseudomonas putida in Complex with Uridine-diphosphate-2(n-acetylglucosaminyl) butyric acid, (2R)-2-(phosphonooxy)propanoic acid and Magnesium
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0008360 | biological_process | regulation of cell shape |
| A | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| A | 0009252 | biological_process | peptidoglycan biosynthetic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| A | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| A | 0051301 | biological_process | cell division |
| A | 0071555 | biological_process | cell wall organization |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0008360 | biological_process | regulation of cell shape |
| B | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| B | 0009252 | biological_process | peptidoglycan biosynthetic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| B | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| B | 0051301 | biological_process | cell division |
| B | 0071555 | biological_process | cell wall organization |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0008360 | biological_process | regulation of cell shape |
| C | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| C | 0009252 | biological_process | peptidoglycan biosynthetic process |
| C | 0016740 | molecular_function | transferase activity |
| C | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| C | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| C | 0051301 | biological_process | cell division |
| C | 0071555 | biological_process | cell wall organization |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0008360 | biological_process | regulation of cell shape |
| D | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| D | 0009252 | biological_process | peptidoglycan biosynthetic process |
| D | 0016740 | molecular_function | transferase activity |
| D | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| D | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| D | 0051301 | biological_process | cell division |
| D | 0071555 | biological_process | cell wall organization |
| E | 0003824 | molecular_function | catalytic activity |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0008360 | biological_process | regulation of cell shape |
| E | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| E | 0009252 | biological_process | peptidoglycan biosynthetic process |
| E | 0016740 | molecular_function | transferase activity |
| E | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| E | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| E | 0051301 | biological_process | cell division |
| E | 0071555 | biological_process | cell wall organization |
| F | 0003824 | molecular_function | catalytic activity |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0008360 | biological_process | regulation of cell shape |
| F | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| F | 0009252 | biological_process | peptidoglycan biosynthetic process |
| F | 0016740 | molecular_function | transferase activity |
| F | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| F | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| F | 0051301 | biological_process | cell division |
| F | 0071555 | biological_process | cell wall organization |
| G | 0003824 | molecular_function | catalytic activity |
| G | 0005737 | cellular_component | cytoplasm |
| G | 0008360 | biological_process | regulation of cell shape |
| G | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| G | 0009252 | biological_process | peptidoglycan biosynthetic process |
| G | 0016740 | molecular_function | transferase activity |
| G | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| G | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| G | 0051301 | biological_process | cell division |
| G | 0071555 | biological_process | cell wall organization |
| H | 0003824 | molecular_function | catalytic activity |
| H | 0005737 | cellular_component | cytoplasm |
| H | 0008360 | biological_process | regulation of cell shape |
| H | 0008760 | molecular_function | UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity |
| H | 0009252 | biological_process | peptidoglycan biosynthetic process |
| H | 0016740 | molecular_function | transferase activity |
| H | 0016765 | molecular_function | transferase activity, transferring alkyl or aryl (other than methyl) groups |
| H | 0019277 | biological_process | UDP-N-acetylgalactosamine biosynthetic process |
| H | 0051301 | biological_process | cell division |
| H | 0071555 | biological_process | cell wall organization |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 20 |
| Details | binding site for residue EPU A 501 |
| Chain | Residue |
| A | LYS22 |
| A | VAL164 |
| A | SER165 |
| A | VAL166 |
| A | THR167 |
| A | THR307 |
| A | ASP308 |
| A | ILE330 |
| A | PHE331 |
| A | LEU373 |
| A | HOH601 |
| A | ASN23 |
| A | HOH605 |
| A | ALA94 |
| A | SER121 |
| A | ARG122 |
| A | PRO123 |
| A | VAL124 |
| A | ASP125 |
| A | LEU126 |
| site_id | AC2 |
| Number of Residues | 9 |
| Details | binding site for residue 0V5 A 502 |
| Chain | Residue |
| A | ARG93 |
| A | CYS117 |
| A | ALA118 |
| A | ILE119 |
| A | ARG400 |
| A | MG503 |
| A | HOH602 |
| A | HOH606 |
| A | HOH607 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | binding site for residue MG A 503 |
| Chain | Residue |
| A | 0V5502 |
| A | HOH602 |
| A | HOH607 |
| A | HOH636 |
| A | HOH639 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | binding site for residue CL A 504 |
| Chain | Residue |
| A | GLU263 |
| A | HOH638 |
| site_id | AC5 |
| Number of Residues | 18 |
| Details | binding site for residue EPU B 500 |
| Chain | Residue |
| B | LYS22 |
| B | ASN23 |
| B | ALA94 |
| B | SER121 |
| B | ARG122 |
| B | PRO123 |
| B | VAL124 |
| B | ASP125 |
| B | LEU126 |
| B | THR163 |
| B | SER165 |
| B | VAL166 |
| B | THR167 |
| B | ASP308 |
| B | ILE330 |
| B | PHE331 |
| B | LEU373 |
| B | HOH601 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | binding site for residue MG B 502 |
| Chain | Residue |
| B | ARG400 |
| B | 0V5501 |
| B | HOH609 |
| B | HOH615 |
| B | HOH630 |
| B | HOH632 |
| site_id | AC7 |
| Number of Residues | 19 |
| Details | binding site for residue EPU C 501 |
| Chain | Residue |
| C | LYS22 |
| C | ASN23 |
| C | ALA94 |
| C | SER121 |
| C | ARG122 |
| C | PRO123 |
| C | VAL124 |
| C | ASP125 |
| C | LEU126 |
| C | THR163 |
| C | SER165 |
| C | VAL166 |
| C | THR167 |
| C | THR307 |
| C | ASP308 |
| C | ILE330 |
| C | PHE331 |
| C | LEU373 |
| C | HOH603 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | binding site for residue MG C 503 |
| Chain | Residue |
| C | 0V5502 |
| C | HOH601 |
| C | HOH619 |
| C | HOH624 |
| C | HOH643 |
| site_id | AC9 |
| Number of Residues | 2 |
| Details | binding site for residue CL C 504 |
| Chain | Residue |
| C | SER121 |
| C | GLU332 |
| site_id | AD1 |
| Number of Residues | 20 |
| Details | binding site for residue EPU D 500 |
| Chain | Residue |
| D | THR307 |
| D | ASP308 |
| D | ILE330 |
| D | PHE331 |
| D | LEU373 |
| D | HOH615 |
| D | HOH629 |
| D | LYS22 |
| D | ASN23 |
| D | SER121 |
| D | ARG122 |
| D | PRO123 |
| D | VAL124 |
| D | ASP125 |
| D | LEU126 |
| D | THR163 |
| D | VAL164 |
| D | SER165 |
| D | VAL166 |
| D | THR167 |
| site_id | AD2 |
| Number of Residues | 6 |
| Details | binding site for residue MG D 502 |
| Chain | Residue |
| D | ARG400 |
| D | 0V5501 |
| D | HOH601 |
| D | HOH627 |
| D | HOH631 |
| D | HOH635 |
| site_id | AD3 |
| Number of Residues | 18 |
| Details | binding site for residue EPU E 500 |
| Chain | Residue |
| E | LYS22 |
| E | ASN23 |
| E | ALA94 |
| E | SER121 |
| E | ARG122 |
| E | PRO123 |
| E | VAL124 |
| E | ASP125 |
| E | LEU126 |
| E | HIS127 |
| E | THR163 |
| E | SER165 |
| E | VAL166 |
| E | THR167 |
| E | ASP308 |
| E | ILE330 |
| E | PHE331 |
| E | LEU373 |
| site_id | AD4 |
| Number of Residues | 5 |
| Details | binding site for residue MG E 502 |
| Chain | Residue |
| E | 0V5501 |
| E | HOH602 |
| E | HOH608 |
| E | HOH627 |
| E | HOH631 |
| site_id | AD5 |
| Number of Residues | 18 |
| Details | binding site for residue EPU F 501 |
| Chain | Residue |
| F | LYS22 |
| F | ASN23 |
| F | ALA94 |
| F | SER121 |
| F | ARG122 |
| F | PRO123 |
| F | VAL124 |
| F | ASP125 |
| F | LEU126 |
| F | THR163 |
| F | SER165 |
| F | VAL166 |
| F | THR167 |
| F | THR307 |
| F | ASP308 |
| F | ILE330 |
| F | PHE331 |
| F | HOH617 |
| site_id | AD6 |
| Number of Residues | 6 |
| Details | binding site for residue MG F 503 |
| Chain | Residue |
| F | ARG400 |
| F | 0V5502 |
| F | HOH601 |
| F | HOH602 |
| F | HOH622 |
| F | HOH628 |
| site_id | AD7 |
| Number of Residues | 3 |
| Details | binding site for residue CL F 504 |
| Chain | Residue |
| F | GLN410 |
| F | ARG417 |
| F | ARG418 |
| site_id | AD8 |
| Number of Residues | 20 |
| Details | binding site for residue EPU G 500 |
| Chain | Residue |
| G | LYS22 |
| G | ASN23 |
| G | ALA94 |
| G | SER121 |
| G | ARG122 |
| G | PRO123 |
| G | VAL124 |
| G | ASP125 |
| G | LEU126 |
| G | VAL164 |
| G | SER165 |
| G | VAL166 |
| G | THR167 |
| G | THR307 |
| G | ASP308 |
| G | ILE330 |
| G | PHE331 |
| G | LEU373 |
| G | HOH603 |
| G | HOH610 |
| site_id | AD9 |
| Number of Residues | 5 |
| Details | binding site for residue MG G 502 |
| Chain | Residue |
| G | 0V5501 |
| G | HOH601 |
| G | HOH611 |
| G | HOH613 |
| G | HOH617 |
| site_id | AE1 |
| Number of Residues | 19 |
| Details | binding site for residue EPU H 501 |
| Chain | Residue |
| H | LYS22 |
| H | ASN23 |
| H | SER121 |
| H | ARG122 |
| H | PRO123 |
| H | VAL124 |
| H | ASP125 |
| H | LEU126 |
| H | THR163 |
| H | SER165 |
| H | VAL166 |
| H | THR167 |
| H | THR307 |
| H | ASP308 |
| H | ILE330 |
| H | PHE331 |
| H | LEU373 |
| H | HOH601 |
| H | HOH627 |
| site_id | AE2 |
| Number of Residues | 6 |
| Details | binding site for residue MG H 503 |
| Chain | Residue |
| H | ARG400 |
| H | 0V5502 |
| H | HOH604 |
| H | HOH614 |
| H | HOH624 |
| H | HOH641 |
| site_id | AE3 |
| Number of Residues | 1 |
| Details | binding site for residue CL H 504 |
| Chain | Residue |
| H | ARG77 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | ACT_SITE: Proton donor => ECO:0000255|HAMAP-Rule:MF_00111 |
| Chain | Residue | Details |
| A | CYS117 | |
| B | CYS117 | |
| C | CYS117 | |
| D | CYS117 | |
| E | CYS117 | |
| F | CYS117 | |
| G | CYS117 | |
| H | CYS117 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 40 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00111 |
| Chain | Residue | Details |
| D | ARG93 | |
| D | ARG122 | |
| D | ASP308 | |
| D | ILE330 | |
| E | LYS22 | |
| E | ARG93 | |
| E | ARG122 | |
| E | ASP308 | |
| E | ILE330 | |
| F | LYS22 | |
| F | ARG93 | |
| F | ARG122 | |
| F | ASP308 | |
| F | ILE330 | |
| G | LYS22 | |
| G | ARG93 | |
| G | ARG122 | |
| G | ASP308 | |
| G | ILE330 | |
| H | LYS22 | |
| H | ARG93 | |
| H | ARG122 | |
| H | ASP308 | |
| H | ILE330 | |
| A | LYS22 | |
| A | ARG93 | |
| B | ILE330 | |
| C | LYS22 | |
| C | ARG93 | |
| C | ARG122 | |
| C | ASP308 | |
| C | ILE330 | |
| D | LYS22 | |
| A | ARG122 | |
| A | ASP308 | |
| A | ILE330 | |
| B | LYS22 | |
| B | ARG93 | |
| B | ARG122 | |
| B | ASP308 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | MOD_RES: 2-(S-cysteinyl)pyruvic acid O-phosphothioketal => ECO:0000255|HAMAP-Rule:MF_00111 |
| Chain | Residue | Details |
| B | CYS117 | |
| C | CYS117 | |
| D | CYS117 | |
| E | CYS117 | |
| F | CYS117 | |
| G | CYS117 | |
| H | CYS117 | |
| A | CYS117 |
