6CCE
Crystal structure of a Mycobacterium smegmatis RNA polymerase transcription initiation complex with inhibitor Kanglemycin A
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000428 | cellular_component | DNA-directed RNA polymerase complex |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003899 | molecular_function | DNA-directed RNA polymerase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006351 | biological_process | DNA-templated transcription |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016779 | molecular_function | nucleotidyltransferase activity |
| A | 0034062 | molecular_function | 5'-3' RNA polymerase activity |
| A | 0046983 | molecular_function | protein dimerization activity |
| B | 0000428 | cellular_component | DNA-directed RNA polymerase complex |
| B | 0003677 | molecular_function | DNA binding |
| B | 0003899 | molecular_function | DNA-directed RNA polymerase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0006351 | biological_process | DNA-templated transcription |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016779 | molecular_function | nucleotidyltransferase activity |
| B | 0034062 | molecular_function | 5'-3' RNA polymerase activity |
| B | 0046983 | molecular_function | protein dimerization activity |
| C | 0000428 | cellular_component | DNA-directed RNA polymerase complex |
| C | 0003677 | molecular_function | DNA binding |
| C | 0003899 | molecular_function | DNA-directed RNA polymerase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0006351 | biological_process | DNA-templated transcription |
| C | 0016740 | molecular_function | transferase activity |
| C | 0016779 | molecular_function | nucleotidyltransferase activity |
| C | 0032549 | molecular_function | ribonucleoside binding |
| C | 0034062 | molecular_function | 5'-3' RNA polymerase activity |
| C | 0046677 | biological_process | response to antibiotic |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0000428 | cellular_component | DNA-directed RNA polymerase complex |
| D | 0003677 | molecular_function | DNA binding |
| D | 0003899 | molecular_function | DNA-directed RNA polymerase activity |
| D | 0006351 | biological_process | DNA-templated transcription |
| D | 0008270 | molecular_function | zinc ion binding |
| D | 0016740 | molecular_function | transferase activity |
| D | 0016779 | molecular_function | nucleotidyltransferase activity |
| D | 0034062 | molecular_function | 5'-3' RNA polymerase activity |
| D | 0046872 | molecular_function | metal ion binding |
| E | 0000428 | cellular_component | DNA-directed RNA polymerase complex |
| E | 0003677 | molecular_function | DNA binding |
| E | 0003899 | molecular_function | DNA-directed RNA polymerase activity |
| E | 0006351 | biological_process | DNA-templated transcription |
| E | 0016740 | molecular_function | transferase activity |
| E | 0016779 | molecular_function | nucleotidyltransferase activity |
| E | 0034062 | molecular_function | 5'-3' RNA polymerase activity |
| F | 0003677 | molecular_function | DNA binding |
| F | 0003700 | molecular_function | DNA-binding transcription factor activity |
| F | 0005737 | cellular_component | cytoplasm |
| F | 0006352 | biological_process | DNA-templated transcription initiation |
| F | 0006355 | biological_process | regulation of DNA-templated transcription |
| F | 0010468 | biological_process | regulation of gene expression |
| F | 0016987 | molecular_function | sigma factor activity |
| F | 2000142 | biological_process | regulation of DNA-templated transcription initiation |
| J | 0001000 | molecular_function | bacterial-type RNA polymerase core enzyme binding |
| J | 0045893 | biological_process | positive regulation of DNA-templated transcription |
| J | 0046677 | biological_process | response to antibiotic |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 18 |
| Details | binding site for residue KNG C 1201 |
| Chain | Residue |
| C | ARG164 |
| C | HIS442 |
| C | ARG445 |
| C | SER447 |
| C | LEU449 |
| C | ARG456 |
| C | PRO480 |
| C | ILE488 |
| C | ARG604 |
| F | GLU368 |
| C | GLY423 |
| C | THR424 |
| C | SER425 |
| C | GLN426 |
| C | SER428 |
| C | GLN429 |
| C | PHE430 |
| C | ASP432 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 C 1202 |
| Chain | Residue |
| C | ARG595 |
| C | ARG915 |
| C | ARG916 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 C 1203 |
| Chain | Residue |
| B | TYR32 |
| C | HIS720 |
| C | ARG1005 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue ZN D 2001 |
| Chain | Residue |
| D | CYS890 |
| D | ARG962 |
| D | CYS967 |
| D | CYS974 |
| D | CYS977 |
| site_id | AC5 |
| Number of Residues | 3 |
| Details | binding site for residue MG D 2002 |
| Chain | Residue |
| D | ASP535 |
| D | ASP537 |
| D | ASP539 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | binding site for residue GLU D 2003 |
| Chain | Residue |
| D | ARG886 |
| D | ILE1265 |
| D | SER1266 |
| D | ARG1267 |
| D | ARG1269 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 D 2004 |
| Chain | Residue |
| C | GLY1050 |
| D | ARG421 |
| D | ARG427 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 D 2005 |
| Chain | Residue |
| D | GLU887 |
| D | HIS888 |
| D | ARG1267 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 D 2006 |
| Chain | Residue |
| D | ASP882 |
| D | VAL883 |
| D | THR971 |
| D | GLY972 |
| D | ARG1160 |
| D | GLN1161 |
| D | ARG1164 |
| site_id | AD1 |
| Number of Residues | 5 |
| Details | binding site for residue SO4 D 2007 |
| Chain | Residue |
| D | GLU893 |
| D | ARG894 |
| D | GLY895 |
| D | LYS960 |
| D | ARG1129 |
| site_id | AD2 |
| Number of Residues | 2 |
| Details | binding site for residue SO4 F 501 |
| Chain | Residue |
| F | ALA254 |
| F | PHE255 |
| site_id | AD3 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 F 502 |
| Chain | Residue |
| C | TYR1012 |
| D | VAL592 |
| D | SER628 |
| D | ARG630 |
| F | LYS210 |
| F | LEU211 |
| F | VAL213 |
| site_id | AD4 |
| Number of Residues | 3 |
| Details | binding site for residue EDO F 503 |
| Chain | Residue |
| F | ARG290 |
| F | THR294 |
| O | DA32 |
| site_id | AD5 |
| Number of Residues | 3 |
| Details | binding site for residue EDO F 504 |
| Chain | Residue |
| D | ARG356 |
| F | GLN291 |
| F | ALA292 |
| site_id | AD6 |
| Number of Residues | 3 |
| Details | binding site for residue EDO F 505 |
| Chain | Residue |
| F | ARG224 |
| F | ASP225 |
| F | ARG228 |
Functional Information from PROSITE/UniProt
| site_id | PS00715 |
| Number of Residues | 14 |
| Details | SIGMA70_1 Sigma-70 factors family signature 1. DLIQeGnLGLIrAV |
| Chain | Residue | Details |
| F | ASP257-VAL270 |
| site_id | PS00716 |
| Number of Residues | 27 |
| Details | SIGMA70_2 Sigma-70 factors family signature 2. TldEIGqvygVTrerIrQIEsktMskL |
| Chain | Residue | Details |
| F | THR426-LEU452 |
| site_id | PS00867 |
| Number of Residues | 8 |
| Details | CPSASE_2 Carbamoyl-phosphate synthase subdomain signature 2. LLEANLRL |
| Chain | Residue | Details |
| F | LEU233-LEU240 |
| site_id | PS01166 |
| Number of Residues | 13 |
| Details | RNA_POL_BETA RNA polymerases beta chain signature. GdKLAGrHGNKGV |
| Chain | Residue | Details |
| C | GLY873-VAL885 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 7 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01322","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
