Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6BNV

CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state, backbone-averaged with side chains truncated to alanine

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0001725	cellular_component	stress fiber
A	0003785	molecular_function	actin monomer binding
A	0005509	molecular_function	calcium ion binding
A	0005515	molecular_function	protein binding
A	0005523	molecular_function	tropomyosin binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005865	cellular_component	striated muscle thin filament
A	0005884	cellular_component	actin filament
A	0010628	biological_process	positive regulation of gene expression
A	0016787	molecular_function	hydrolase activity
A	0019904	molecular_function	protein domain specific binding
A	0030027	cellular_component	lamellipodium
A	0030041	biological_process	actin filament polymerization
A	0030175	cellular_component	filopodium
A	0030240	biological_process	skeletal muscle thin filament assembly
A	0031013	molecular_function	troponin I binding
A	0031432	molecular_function	titin binding
A	0031941	cellular_component	filamentous actin
A	0032036	molecular_function	myosin heavy chain binding
A	0032432	cellular_component	actin filament bundle
A	0042802	molecular_function	identical protein binding
A	0044297	cellular_component	cell body
A	0048306	molecular_function	calcium-dependent protein binding
A	0048741	biological_process	skeletal muscle fiber development
A	0051017	biological_process	actin filament bundle assembly
A	0090131	biological_process	mesenchyme migration
A	0098723	cellular_component	skeletal muscle myofibril
A	0140660	molecular_function	cytoskeletal motor activator activity
B	0000287	molecular_function	magnesium ion binding
B	0001725	cellular_component	stress fiber
B	0003785	molecular_function	actin monomer binding
B	0005509	molecular_function	calcium ion binding
B	0005515	molecular_function	protein binding
B	0005523	molecular_function	tropomyosin binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005856	cellular_component	cytoskeleton
B	0005865	cellular_component	striated muscle thin filament
B	0005884	cellular_component	actin filament
B	0010628	biological_process	positive regulation of gene expression
B	0016787	molecular_function	hydrolase activity
B	0019904	molecular_function	protein domain specific binding
B	0030027	cellular_component	lamellipodium
B	0030041	biological_process	actin filament polymerization
B	0030175	cellular_component	filopodium
B	0030240	biological_process	skeletal muscle thin filament assembly
B	0031013	molecular_function	troponin I binding
B	0031432	molecular_function	titin binding
B	0031941	cellular_component	filamentous actin
B	0032036	molecular_function	myosin heavy chain binding
B	0032432	cellular_component	actin filament bundle
B	0042802	molecular_function	identical protein binding
B	0044297	cellular_component	cell body
B	0048306	molecular_function	calcium-dependent protein binding
B	0048741	biological_process	skeletal muscle fiber development
B	0051017	biological_process	actin filament bundle assembly
B	0090131	biological_process	mesenchyme migration
B	0098723	cellular_component	skeletal muscle myofibril
B	0140660	molecular_function	cytoskeletal motor activator activity
C	0000287	molecular_function	magnesium ion binding
C	0001725	cellular_component	stress fiber
C	0003785	molecular_function	actin monomer binding
C	0005509	molecular_function	calcium ion binding
C	0005515	molecular_function	protein binding
C	0005523	molecular_function	tropomyosin binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005856	cellular_component	cytoskeleton
C	0005865	cellular_component	striated muscle thin filament
C	0005884	cellular_component	actin filament
C	0010628	biological_process	positive regulation of gene expression
C	0016787	molecular_function	hydrolase activity
C	0019904	molecular_function	protein domain specific binding
C	0030027	cellular_component	lamellipodium
C	0030041	biological_process	actin filament polymerization
C	0030175	cellular_component	filopodium
C	0030240	biological_process	skeletal muscle thin filament assembly
C	0031013	molecular_function	troponin I binding
C	0031432	molecular_function	titin binding
C	0031941	cellular_component	filamentous actin
C	0032036	molecular_function	myosin heavy chain binding
C	0032432	cellular_component	actin filament bundle
C	0042802	molecular_function	identical protein binding
C	0044297	cellular_component	cell body
C	0048306	molecular_function	calcium-dependent protein binding
C	0048741	biological_process	skeletal muscle fiber development
C	0051017	biological_process	actin filament bundle assembly
C	0090131	biological_process	mesenchyme migration
C	0098723	cellular_component	skeletal muscle myofibril
C	0140660	molecular_function	cytoskeletal motor activator activity
D	0000287	molecular_function	magnesium ion binding
D	0001725	cellular_component	stress fiber
D	0003785	molecular_function	actin monomer binding
D	0005509	molecular_function	calcium ion binding
D	0005515	molecular_function	protein binding
D	0005523	molecular_function	tropomyosin binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0005865	cellular_component	striated muscle thin filament
D	0005884	cellular_component	actin filament
D	0010628	biological_process	positive regulation of gene expression
D	0016787	molecular_function	hydrolase activity
D	0019904	molecular_function	protein domain specific binding
D	0030027	cellular_component	lamellipodium
D	0030041	biological_process	actin filament polymerization
D	0030175	cellular_component	filopodium
D	0030240	biological_process	skeletal muscle thin filament assembly
D	0031013	molecular_function	troponin I binding
D	0031432	molecular_function	titin binding
D	0031941	cellular_component	filamentous actin
D	0032036	molecular_function	myosin heavy chain binding
D	0032432	cellular_component	actin filament bundle
D	0042802	molecular_function	identical protein binding
D	0044297	cellular_component	cell body
D	0048306	molecular_function	calcium-dependent protein binding
D	0048741	biological_process	skeletal muscle fiber development
D	0051017	biological_process	actin filament bundle assembly
D	0090131	biological_process	mesenchyme migration
D	0098723	cellular_component	skeletal muscle myofibril
D	0140660	molecular_function	cytoskeletal motor activator activity
E	0000287	molecular_function	magnesium ion binding
E	0001725	cellular_component	stress fiber
E	0003785	molecular_function	actin monomer binding
E	0005509	molecular_function	calcium ion binding
E	0005515	molecular_function	protein binding
E	0005523	molecular_function	tropomyosin binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005856	cellular_component	cytoskeleton
E	0005865	cellular_component	striated muscle thin filament
E	0005884	cellular_component	actin filament
E	0010628	biological_process	positive regulation of gene expression
E	0016787	molecular_function	hydrolase activity
E	0019904	molecular_function	protein domain specific binding
E	0030027	cellular_component	lamellipodium
E	0030041	biological_process	actin filament polymerization
E	0030175	cellular_component	filopodium
E	0030240	biological_process	skeletal muscle thin filament assembly
E	0031013	molecular_function	troponin I binding
E	0031432	molecular_function	titin binding
E	0031941	cellular_component	filamentous actin
E	0032036	molecular_function	myosin heavy chain binding
E	0032432	cellular_component	actin filament bundle
E	0042802	molecular_function	identical protein binding
E	0044297	cellular_component	cell body
E	0048306	molecular_function	calcium-dependent protein binding
E	0048741	biological_process	skeletal muscle fiber development
E	0051017	biological_process	actin filament bundle assembly
E	0090131	biological_process	mesenchyme migration
E	0098723	cellular_component	skeletal muscle myofibril
E	0140660	molecular_function	cytoskeletal motor activator activity
F	0000287	molecular_function	magnesium ion binding
F	0001725	cellular_component	stress fiber
F	0003785	molecular_function	actin monomer binding
F	0005509	molecular_function	calcium ion binding
F	0005515	molecular_function	protein binding
F	0005523	molecular_function	tropomyosin binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005856	cellular_component	cytoskeleton
F	0005865	cellular_component	striated muscle thin filament
F	0005884	cellular_component	actin filament
F	0010628	biological_process	positive regulation of gene expression
F	0016787	molecular_function	hydrolase activity
F	0019904	molecular_function	protein domain specific binding
F	0030027	cellular_component	lamellipodium
F	0030041	biological_process	actin filament polymerization
F	0030175	cellular_component	filopodium
F	0030240	biological_process	skeletal muscle thin filament assembly
F	0031013	molecular_function	troponin I binding
F	0031432	molecular_function	titin binding
F	0031941	cellular_component	filamentous actin
F	0032036	molecular_function	myosin heavy chain binding
F	0032432	cellular_component	actin filament bundle
F	0042802	molecular_function	identical protein binding
F	0044297	cellular_component	cell body
F	0048306	molecular_function	calcium-dependent protein binding
F	0048741	biological_process	skeletal muscle fiber development
F	0051017	biological_process	actin filament bundle assembly
F	0090131	biological_process	mesenchyme migration
F	0098723	cellular_component	skeletal muscle myofibril
F	0140660	molecular_function	cytoskeletal motor activator activity
G	0000287	molecular_function	magnesium ion binding
G	0001725	cellular_component	stress fiber
G	0003785	molecular_function	actin monomer binding
G	0005509	molecular_function	calcium ion binding
G	0005515	molecular_function	protein binding
G	0005523	molecular_function	tropomyosin binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005856	cellular_component	cytoskeleton
G	0005865	cellular_component	striated muscle thin filament
G	0005884	cellular_component	actin filament
G	0010628	biological_process	positive regulation of gene expression
G	0016787	molecular_function	hydrolase activity
G	0019904	molecular_function	protein domain specific binding
G	0030027	cellular_component	lamellipodium
G	0030041	biological_process	actin filament polymerization
G	0030175	cellular_component	filopodium
G	0030240	biological_process	skeletal muscle thin filament assembly
G	0031013	molecular_function	troponin I binding
G	0031432	molecular_function	titin binding
G	0031941	cellular_component	filamentous actin
G	0032036	molecular_function	myosin heavy chain binding
G	0032432	cellular_component	actin filament bundle
G	0042802	molecular_function	identical protein binding
G	0044297	cellular_component	cell body
G	0048306	molecular_function	calcium-dependent protein binding
G	0048741	biological_process	skeletal muscle fiber development
G	0051017	biological_process	actin filament bundle assembly
G	0090131	biological_process	mesenchyme migration
G	0098723	cellular_component	skeletal muscle myofibril
G	0140660	molecular_function	cytoskeletal motor activator activity
H	0000287	molecular_function	magnesium ion binding
H	0001725	cellular_component	stress fiber
H	0003785	molecular_function	actin monomer binding
H	0005509	molecular_function	calcium ion binding
H	0005515	molecular_function	protein binding
H	0005523	molecular_function	tropomyosin binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005856	cellular_component	cytoskeleton
H	0005865	cellular_component	striated muscle thin filament
H	0005884	cellular_component	actin filament
H	0010628	biological_process	positive regulation of gene expression
H	0016787	molecular_function	hydrolase activity
H	0019904	molecular_function	protein domain specific binding
H	0030027	cellular_component	lamellipodium
H	0030041	biological_process	actin filament polymerization
H	0030175	cellular_component	filopodium
H	0030240	biological_process	skeletal muscle thin filament assembly
H	0031013	molecular_function	troponin I binding
H	0031432	molecular_function	titin binding
H	0031941	cellular_component	filamentous actin
H	0032036	molecular_function	myosin heavy chain binding
H	0032432	cellular_component	actin filament bundle
H	0042802	molecular_function	identical protein binding
H	0044297	cellular_component	cell body
H	0048306	molecular_function	calcium-dependent protein binding
H	0048741	biological_process	skeletal muscle fiber development
H	0051017	biological_process	actin filament bundle assembly
H	0090131	biological_process	mesenchyme migration
H	0098723	cellular_component	skeletal muscle myofibril
H	0140660	molecular_function	cytoskeletal motor activator activity
I	0003774	molecular_function	cytoskeletal motor activity
I	0005524	molecular_function	ATP binding
I	0016459	cellular_component	myosin complex
I	0051015	molecular_function	actin filament binding
J	0003774	molecular_function	cytoskeletal motor activity
J	0005524	molecular_function	ATP binding
J	0016459	cellular_component	myosin complex
J	0051015	molecular_function	actin filament binding
K	0003774	molecular_function	cytoskeletal motor activity
K	0005524	molecular_function	ATP binding
K	0016459	cellular_component	myosin complex
K	0051015	molecular_function	actin filament binding
L	0003774	molecular_function	cytoskeletal motor activity
L	0005524	molecular_function	ATP binding
L	0016459	cellular_component	myosin complex
L	0051015	molecular_function	actin filament binding
M	0003774	molecular_function	cytoskeletal motor activity
M	0005524	molecular_function	ATP binding
M	0016459	cellular_component	myosin complex
M	0051015	molecular_function	actin filament binding
N	0003774	molecular_function	cytoskeletal motor activity
N	0005524	molecular_function	ATP binding
N	0016459	cellular_component	myosin complex
N	0051015	molecular_function	actin filament binding
O	0005509	molecular_function	calcium ion binding
O	0005515	molecular_function	protein binding
O	0005737	cellular_component	cytoplasm
O	0017022	molecular_function	myosin binding
O	0032991	cellular_component	protein-containing complex
O	0046872	molecular_function	metal ion binding
O	0051401	molecular_function	CH domain binding
O	0097718	molecular_function	disordered domain specific binding
P	0005509	molecular_function	calcium ion binding
P	0005515	molecular_function	protein binding
P	0005737	cellular_component	cytoplasm
P	0017022	molecular_function	myosin binding
P	0032991	cellular_component	protein-containing complex
P	0046872	molecular_function	metal ion binding
P	0051401	molecular_function	CH domain binding
P	0097718	molecular_function	disordered domain specific binding
Q	0005509	molecular_function	calcium ion binding
Q	0005515	molecular_function	protein binding
Q	0005737	cellular_component	cytoplasm
Q	0017022	molecular_function	myosin binding
Q	0032991	cellular_component	protein-containing complex
Q	0046872	molecular_function	metal ion binding
Q	0051401	molecular_function	CH domain binding
Q	0097718	molecular_function	disordered domain specific binding
R	0005509	molecular_function	calcium ion binding
R	0005515	molecular_function	protein binding
R	0005737	cellular_component	cytoplasm
R	0017022	molecular_function	myosin binding
R	0032991	cellular_component	protein-containing complex
R	0046872	molecular_function	metal ion binding
R	0051401	molecular_function	CH domain binding
R	0097718	molecular_function	disordered domain specific binding
S	0005509	molecular_function	calcium ion binding
S	0005515	molecular_function	protein binding
S	0005737	cellular_component	cytoplasm
S	0017022	molecular_function	myosin binding
S	0032991	cellular_component	protein-containing complex
S	0046872	molecular_function	metal ion binding
S	0051401	molecular_function	CH domain binding
S	0097718	molecular_function	disordered domain specific binding
T	0005509	molecular_function	calcium ion binding
T	0005515	molecular_function	protein binding
T	0005737	cellular_component	cytoplasm
T	0017022	molecular_function	myosin binding
T	0032991	cellular_component	protein-containing complex
T	0046872	molecular_function	metal ion binding
T	0051401	molecular_function	CH domain binding
T	0097718	molecular_function	disordered domain specific binding

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL

Chain	Residue	Details
O	ASP20-LEU32
O	ASP56-PHE68
O	ASP93-LEU105
O	ASP129-PHE141

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
A	TYR53-GLY63

site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WITKqEYDE

Chain	Residue	Details
A	TRP356-GLU364

site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApLNPkaNR

Chain	Residue	Details
A	LEU104-ARG116

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255

Chain	Residue	Details
I	GLY151
O	GLU67
S	GLU140
T	ASP20
T	ASP22
T	ASP24
T	THR26
T	GLU31
T	ASP56
T	ASP58
T	ASN60
T	THR62
O	ASP93
T	GLU67
T	ASP93
T	ASP95
T	ASN97
T	TYR99
T	GLU104
T	ASP129
T	ASP131
T	ASP133
T	GLN135
O	ASP95
T	GLU140
O	ASN97
O	TYR99
O	GLU104
O	ASP129
O	ASP131
O	ASP133
O	GLN135
J	GLY151
O	GLU140
P	ASP20
P	ASP22
P	ASP24
P	THR26
P	GLU31
P	ASP56
P	ASP58
P	ASN60
P	THR62
K	GLY151
P	GLU67
P	ASP93
P	ASP95
P	ASN97
P	TYR99
P	GLU104
P	ASP129
P	ASP131
P	ASP133
P	GLN135
L	GLY151
P	GLU140
Q	ASP20
Q	ASP22
Q	ASP24
Q	THR26
Q	GLU31
Q	ASP56
Q	ASP58
Q	ASN60
Q	THR62
M	GLY151
Q	GLU67
Q	ASP93
Q	ASP95
Q	ASN97
Q	TYR99
Q	GLU104
Q	ASP129
Q	ASP131
Q	ASP133
Q	GLN135
N	GLY151
Q	GLU140
R	ASP20
R	ASP22
R	ASP24
R	THR26
R	GLU31
R	ASP56
R	ASP58
R	ASN60
R	THR62
O	ASP58
R	GLU67
R	ASP93
R	ASP95
R	ASN97
R	TYR99
R	GLU104
R	ASP129
R	ASP131
R	ASP133
R	GLN135
O	ASN60
R	GLU140
S	ASP20
S	ASP22
S	ASP24
S	THR26
S	GLU31
S	ASP56
S	ASP58
S	ASN60
S	THR62
O	THR62
S	GLU67
S	ASP93
S	ASP95
S	ASN97
S	TYR99
S	GLU104
S	ASP129
S	ASP131
S	ASP133
S	GLN135

site_id	SWS_FT_FI2
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:Q9UM54

Chain	Residue	Details
I	SER267
J	SER267
K	SER267
L	SER267
M	SER267
N	SER267
G	ASP1
H	ASP1

site_id	SWS_FT_FI3
Number of Residues	6
Details	MOD_RES: Phosphothreonine => ECO:0000269\|PubMed:12682054

Chain	Residue	Details
I	VAL406
E	MET47
F	MET44
F	MET47
G	MET44
G	MET47
H	MET44
H	MET47
J	VAL406
K	VAL406
L	VAL406
M	VAL406
N	VAL406
D	MET44
D	MET47
E	MET44

site_id	SWS_FT_FI4
Number of Residues	6
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:Q64331

Chain	Residue	Details
I	LEU605
J	LEU605
K	LEU605
L	LEU605
M	LEU605
N	LEU605
G	LYS61
H	LYS61

site_id	SWS_FT_FI5
Number of Residues	8
Details	MOD_RES: Tele-methylhistidine => ECO:0000269\|PubMed:1150665, ECO:0000269\|PubMed:16905096, ECO:0000269\|PubMed:213279, ECO:0000269\|PubMed:2395459, ECO:0000269\|PubMed:499690, ECO:0007744\|PDB:1ATN, ECO:0007744\|PDB:1NWK

Chain	Residue	Details
A	HIS73
B	HIS73
C	HIS73
D	HIS73
E	HIS73
F	HIS73
G	HIS73
H	HIS73

site_id	SWS_FT_FI6
Number of Residues	8
Details	MOD_RES: N6-methyllysine => ECO:0000250\|UniProtKB:P68133

Chain	Residue	Details
A	LYS84
B	LYS84
C	LYS84
D	LYS84
E	LYS84
F	LYS84
G	LYS84
H	LYS84

site_id	SWS_FT_FI7
Number of Residues	8
Details	MOD_RES: ADP-ribosylarginine; by SpvB => ECO:0000305\|PubMed:16905096

Chain	Residue	Details
A	ARG177
B	ARG177
C	ARG177
D	ARG177
E	ARG177
F	ARG177
G	ARG177
H	ARG177

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)