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6BGI

Cryo-EM structure of the TMEM16A calcium-activated chloride channel in nanodisc

Functional Information from GO Data
ChainGOidnamespacecontents
A0005229molecular_functionintracellularly calcium-gated chloride channel activity
A0005247molecular_functionvoltage-gated chloride channel activity
A0005254molecular_functionchloride channel activity
A0005515molecular_functionprotein binding
A0005886cellular_componentplasma membrane
A0006821biological_processchloride transport
A0007200biological_processphospholipase C-activating G protein-coupled receptor signaling pathway
A0009897cellular_componentexternal side of plasma membrane
A0016324cellular_componentapical plasma membrane
A0034220biological_processmonoatomic ion transmembrane transport
A0034605biological_processcellular response to heat
A0034707cellular_componentchloride channel complex
A0035774biological_processpositive regulation of insulin secretion involved in cellular response to glucose stimulus
A0042391biological_processregulation of membrane potential
A0042734cellular_componentpresynaptic membrane
A0042802molecular_functionidentical protein binding
A0042803molecular_functionprotein homodimerization activity
A0042995cellular_componentcell projection
A0045202cellular_componentsynapse
A0046872molecular_functionmetal ion binding
A0046983molecular_functionprotein dimerization activity
A0050965biological_processdetection of temperature stimulus involved in sensory perception of pain
A0050966biological_processdetection of mechanical stimulus involved in sensory perception of pain
A0051649biological_processestablishment of localization in cell
A0060438biological_processtrachea development
A0070254biological_processmucus secretion
A0098793cellular_componentpresynapse
A0098978cellular_componentglutamatergic synapse
A0106091biological_processglial cell projection elongation
A1902476biological_processchloride transmembrane transport
B0005229molecular_functionintracellularly calcium-gated chloride channel activity
B0005247molecular_functionvoltage-gated chloride channel activity
B0005254molecular_functionchloride channel activity
B0005515molecular_functionprotein binding
B0005886cellular_componentplasma membrane
B0006821biological_processchloride transport
B0007200biological_processphospholipase C-activating G protein-coupled receptor signaling pathway
B0009897cellular_componentexternal side of plasma membrane
B0016324cellular_componentapical plasma membrane
B0034220biological_processmonoatomic ion transmembrane transport
B0034605biological_processcellular response to heat
B0034707cellular_componentchloride channel complex
B0035774biological_processpositive regulation of insulin secretion involved in cellular response to glucose stimulus
B0042391biological_processregulation of membrane potential
B0042734cellular_componentpresynaptic membrane
B0042802molecular_functionidentical protein binding
B0042803molecular_functionprotein homodimerization activity
B0042995cellular_componentcell projection
B0045202cellular_componentsynapse
B0046872molecular_functionmetal ion binding
B0046983molecular_functionprotein dimerization activity
B0050965biological_processdetection of temperature stimulus involved in sensory perception of pain
B0050966biological_processdetection of mechanical stimulus involved in sensory perception of pain
B0051649biological_processestablishment of localization in cell
B0060438biological_processtrachea development
B0070254biological_processmucus secretion
B0098793cellular_componentpresynapse
B0098978cellular_componentglutamatergic synapse
B0106091biological_processglial cell projection elongation
B1902476biological_processchloride transmembrane transport
Functional Information from PDB Data
site_idAC1
Number of Residues4
Detailsbinding site for residue CA A 1001
ChainResidue
AGLU650
AGLU701
AGLU730
AASP734

site_idAC2
Number of Residues5
Detailsbinding site for residue CA A 1002
ChainResidue
ALEU643
AASN646
AGLU701
AASN726
AGLU730

site_idAC3
Number of Residues3
Detailsbinding site for residue CA B 1001
ChainResidue
BGLU650
BGLU701
BASP734

site_idAC4
Number of Residues5
Detailsbinding site for residue CA B 1002
ChainResidue
BLEU643
BASN646
BGLU701
BASN726
BGLU730

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues952
DetailsTOPO_DOM: Cytoplasmic => ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691
ChainResidueDetails
AMET1-ALA333
BLEU742-ASN758
ALYS428-ILE493
AGLU564-PHE581
AILE653-TYR699
ALEU742-ASN758
BMET1-ALA333
BLYS428-ILE493
BGLU564-PHE581
BILE653-TYR699

site_idSWS_FT_FI2
Number of Residues400
DetailsTRANSMEM: Helical => ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691
ChainResidueDetails
ATRP334-LEU354
APHE867-LYS887
BTRP334-LEU354
BPRO407-TRP427
BILE494-THR514
BLEU543-ILE563
BLEU582-PHE602
BILE632-GLY652
BMET700-PRO720
BLEU721-GLU741
BILE759-SER779
APRO407-TRP427
BPHE867-LYS887
AILE494-THR514
ALEU543-ILE563
ALEU582-PHE602
AILE632-GLY652
AMET700-PRO720
ALEU721-GLU741
AILE759-SER779

site_idSWS_FT_FI3
Number of Residues384
DetailsTOPO_DOM: Extracellular => ECO:0000269|PubMed:29236684, ECO:0000269|PubMed:29236691
ChainResidueDetails
ATYR355-ASN406
AALA515-ASN542
AVAL603-CYS631
AASP780-VAL866
BTYR355-ASN406
BALA515-ASN542
BVAL603-CYS631
BASP780-VAL866

site_idSWS_FT_FI4
Number of Residues6
DetailsBINDING: BINDING => ECO:0000269|PubMed:33378669
ChainResidueDetails
AGLU425
APRO883
AASP888
BGLU425
BPRO883
BASP888

site_idSWS_FT_FI5
Number of Residues12
DetailsBINDING: BINDING => ECO:0000305|PubMed:29236691
ChainResidueDetails
AILE651
BGLN705
BASP734
BPHE738
APRO654
AMET702
AGLN705
AASP734
APHE738
BILE651
BPRO654
BMET702

site_idSWS_FT_FI6
Number of Residues2
DetailsSITE: Unlikely to bind calcium but may play an important structural role => ECO:0000269|PubMed:33378669
ChainResidueDetails
ALYS428
BLYS428

site_idSWS_FT_FI7
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q5XXA6
ChainResidueDetails
ASER196
BSER196

site_idSWS_FT_FI8
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255
ChainResidueDetails
ASER806
BSER806

224572

PDB entries from 2024-09-04

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