6AZN

Structural and biochemical characterization of a non-canonical biuret hydrolase (BiuH) from the cyanuric acid catabolism pathway of Rhizobium leguminasorum bv. viciae 3841

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016787	molecular_function	hydrolase activity
B	0016787	molecular_function	hydrolase activity
C	0016787	molecular_function	hydrolase activity
D	0016787	molecular_function	hydrolase activity
F	0016787	molecular_function	hydrolase activity
G	0016787	molecular_function	hydrolase activity
H	0016787	molecular_function	hydrolase activity
I	0016787	molecular_function	hydrolase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue EDO A 301

Chain	Residue
A	ARG186
A	GLY187
A	TYR188
A	HOH451
A	HOH505

---

site_id	AC2
Number of Residues	6
Details	binding site for residue PO4 A 302

Chain	Residue
B	ARG186
B	TYR188
A	ASN158
A	GLN159
A	ARG161
B	ASN158

---

site_id	AC3
Number of Residues	5
Details	binding site for residue EDO C 301

Chain	Residue
C	ASN158
C	GLN159
C	ARG161
D	ARG186
D	TYR188

---

site_id	AC4
Number of Residues	6
Details	binding site for residue PO4 F 301

Chain	Residue
F	ARG186
F	GLY187
F	TYR188
G	ASN158
G	GLN159
G	ARG161

---

site_id	AC5
Number of Residues	3
Details	binding site for residue EDO I 301

Chain	Residue
I	CYS42
I	GLU130
I	HOH521

---

site_id	AC6
Number of Residues	4
Details	binding site for residue EDO I 302

Chain	Residue
H	ARG186
H	TYR188
I	GLN159
I	ARG161

---

site_id	AC7
Number of Residues	6
Details	binding site for residue PO4 I 303

Chain	Residue
H	ASN158
H	GLN159
H	ARG161
I	ARG186
I	GLY187
I	TYR188

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Proton acceptor => ECO:0000305|PubMed:29425231

Chain	Residue	Details
A	ASP36
B	ASP36
C	ASP36
D	ASP36
F	ASP36
G	ASP36
H	ASP36
I	ASP36

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	ACT_SITE: ACT_SITE => ECO:0000305|PubMed:29425231

Chain	Residue	Details
A	LYS142
B	LYS142
C	LYS142
D	LYS142
F	LYS142
G	LYS142
H	LYS142
I	LYS142

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	ACT_SITE: Nucleophile => ECO:0000305|PubMed:29425231

Chain	Residue	Details
A	SER175
B	SER175
C	SER175
D	SER175
F	SER175
G	SER175
H	SER175
I	SER175

---

site_id	SWS_FT_FI4
Number of Residues	32
Details	BINDING: BINDING => ECO:0000269|PubMed:29425231, ECO:0007744|PDB:6AZQ

Chain	Residue	Details
A	GLU84
C	LYS145
C	ILE170
C	GLN215
D	GLU84
D	LYS145
D	ILE170
D	GLN215
F	GLU84
F	LYS145
F	ILE170
A	LYS145
F	GLN215
G	GLU84
G	LYS145
G	ILE170
G	GLN215
H	GLU84
H	LYS145
H	ILE170
H	GLN215
I	GLU84
A	ILE170
I	LYS145
I	ILE170
I	GLN215
A	GLN215
B	GLU84
B	LYS145
B	ILE170
B	GLN215
C	GLU84

---