6AI5

Disulfide-free, Zn-directed tetramer of the engineered cyt cb562 variant, C96T/A104AB3

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005506	molecular_function	iron ion binding
A	0009055	molecular_function	electron transfer activity
A	0020037	molecular_function	heme binding
A	0022900	biological_process	electron transport chain
A	0042597	cellular_component	periplasmic space
A	0046872	molecular_function	metal ion binding
C	0005506	molecular_function	iron ion binding
C	0009055	molecular_function	electron transfer activity
C	0020037	molecular_function	heme binding
C	0022900	biological_process	electron transport chain
C	0042597	cellular_component	periplasmic space
C	0046872	molecular_function	metal ion binding
E	0005506	molecular_function	iron ion binding
E	0009055	molecular_function	electron transfer activity
E	0020037	molecular_function	heme binding
E	0022900	biological_process	electron transport chain
E	0042597	cellular_component	periplasmic space
E	0046872	molecular_function	metal ion binding
G	0005506	molecular_function	iron ion binding
G	0009055	molecular_function	electron transfer activity
G	0020037	molecular_function	heme binding
G	0022900	biological_process	electron transport chain
G	0042597	cellular_component	periplasmic space
G	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details	binding site for residue HEM A 201

Chain	Residue
A	GLU4
A	TYR105
A	ARG106
A	HOH312
A	MET7
A	ASN11
A	PRO45
A	PRO46
A	PHE61
A	CYS98
A	CYS101
A	HIS102

---

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN A 202

Chain	Residue
A	HIS73
A	HIS77
C	HIS63
E	ASP74

---

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN A 203

Chain	Residue
A	GLU86
A	HIS89
A	CL204
G	HIS100

---

site_id	AC4
Number of Residues	5
Details	binding site for residue CL A 204

Chain	Residue
A	GLU86
A	HIS89
A	ZN203
G	HIS100
G	ALA104

---

site_id	AC5
Number of Residues	3
Details	binding site for residue MG A 205

Chain	Residue
A	GLU8
A	ASP12
E	GLU8

---

site_id	AC6
Number of Residues	4
Details	binding site for residue ZN A 206

Chain	Residue
A	HIS63
C	HIS73
C	HIS77
G	ASP74

---

site_id	AC7
Number of Residues	4
Details	binding site for residue ZN A 207

Chain	Residue
A	ASP74
E	HIS73
E	HIS77
G	HIS63

---

site_id	AC8
Number of Residues	4
Details	binding site for residue ZN A 208

Chain	Residue
A	HIS100
G	GLU86
G	HIS89
G	HOH384

---

site_id	AC9
Number of Residues	4
Details	binding site for residue ZN C 202

Chain	Residue
C	HIS89
E	HIS100
E	HOH316
G	GLU4

---

site_id	AD1
Number of Residues	3
Details	binding site for residue MG C 203

Chain	Residue
C	ASN13
C	HOH318
C	HOH342

---

site_id	AD2
Number of Residues	4
Details	binding site for residue ZN C 204

Chain	Residue
C	HIS100
E	GLU86
E	HIS89
E	HOH371

---

site_id	AD3
Number of Residues	4
Details	binding site for residue ZN C 205

Chain	Residue
C	ASP74
E	HIS63
G	HIS73
G	HIS77

---

site_id	AD4
Number of Residues	2
Details	binding site for residue CL E 202

Chain	Residue
C	GLU86
E	HIS100

---

site_id	AD5
Number of Residues	4
Details	binding site for residue CL E 203

Chain	Residue
A	HIS77
C	HOH389
E	LEU78
E	GLU81

---

site_id	AD6
Number of Residues	4
Details	binding site for residue CL E 204

Chain	Residue
E	TRP41
E	ALA43
E	MET58
E	ARG62

---

site_id	AD7
Number of Residues	5
Details	binding site for residue MG E 205

Chain	Residue
C	GLU8
C	ASN11
E	ASP2
E	ASP5
E	HOH302

---

site_id	AD8
Number of Residues	4
Details	binding site for residue CL G 202

Chain	Residue
G	ALA24
G	ASP28
G	HOH389
G	HOH391

---

site_id	AD9
Number of Residues	5
Details	binding site for residue MG G 203

Chain	Residue
A	HOH343
C	HIS77
C	ASN80
C	HOH397
G	GLU81

---

site_id	AE1
Number of Residues	18
Details	binding site for Di-peptide HEM C 201 and CYS C 98

Chain	Residue
C	GLU4
C	MET7
C	GLU8
C	LEU14
C	PRO45
C	PHE61
C	LEU94
C	LYS95
C	THR96
C	THR97
C	ASN99
C	HIS100
C	CYS101
C	HIS102
C	ARG106
C	HOH313
C	HOH386
E	ASP2

---

site_id	AE2
Number of Residues	18
Details	binding site for Di-peptide HEM C 201 and CYS C 101

Chain	Residue
C	GLU8
C	LEU14
C	PRO45
C	PHE61
C	THR97
C	CYS98
C	ASN99
C	HIS100
C	HIS102
C	GLN103
C	ALA104
C	TYR105
C	ARG106
C	HOH313
C	HOH386
E	ASP2
C	GLU4
C	MET7

---

site_id	AE3
Number of Residues	18
Details	binding site for Di-peptide HEM E 201 and CYS E 101

Chain	Residue
A	GLU8
A	HOH335
E	GLU4
E	MET7
E	GLU8
E	ASN11
E	PRO45
E	PHE61
E	THR97
E	CYS98
E	ASN99
E	HIS100
E	HIS102
E	GLN103
E	ALA104
E	TYR105
E	ARG106
E	HOH328

---

site_id	AE4
Number of Residues	18
Details	binding site for Di-peptide HEM E 201 and CYS E 98

Chain	Residue
A	GLU8
A	HOH335
E	GLU4
E	MET7
E	GLU8
E	ASN11
E	PRO45
E	PHE61
E	LEU94
E	LYS95
E	THR96
E	THR97
E	ASN99
E	HIS100
E	CYS101
E	HIS102
E	ARG106
E	HOH328

---

site_id	AE5
Number of Residues	16
Details	binding site for Di-peptide HEM G 201 and CYS G 101

Chain	Residue
E	GLN103
G	GLU4
G	MET7
G	GLU8
G	PRO45
G	PHE61
G	THR97
G	CYS98
G	ASN99
G	HIS100
G	HIS102
G	GLN103
G	ALA104
G	TYR105
G	ARG106
G	HOH334

---

site_id	AE6
Number of Residues	16
Details	binding site for Di-peptide HEM G 201 and CYS G 98

Chain	Residue
E	GLN103
G	GLU4
G	MET7
G	GLU8
G	PRO45
G	PHE61
G	LEU94
G	LYS95
G	THR96
G	THR97
G	ASN99
G	HIS100
G	CYS101
G	HIS102
G	ARG106
G	HOH334

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	BINDING: axial binding residue

Chain	Residue	Details
A	MET7
A	HIS102
C	MET7
C	HIS102
E	MET7
E	HIS102
G	MET7
G	HIS102

---