Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6AHC

Cryo-EM structure of aldehyde-alcohol dehydrogenase reveals a high-order helical architecture critical for its activity

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
A	0005515	molecular_function	protein binding
A	0005829	cellular_component	cytosol
A	0006066	biological_process	alcohol metabolic process
A	0006115	biological_process	ethanol biosynthetic process
A	0006979	biological_process	response to oxidative stress
A	0008198	molecular_function	ferrous iron binding
A	0008774	molecular_function	acetaldehyde dehydrogenase (acetylating) activity
A	0015976	biological_process	carbon utilization
A	0016020	cellular_component	membrane
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
A	0019664	biological_process	mixed acid fermentation
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
A	0051260	biological_process	protein homooligomerization
B	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
B	0005515	molecular_function	protein binding
B	0005829	cellular_component	cytosol
B	0006066	biological_process	alcohol metabolic process
B	0006115	biological_process	ethanol biosynthetic process
B	0006979	biological_process	response to oxidative stress
B	0008198	molecular_function	ferrous iron binding
B	0008774	molecular_function	acetaldehyde dehydrogenase (acetylating) activity
B	0015976	biological_process	carbon utilization
B	0016020	cellular_component	membrane
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
B	0019664	biological_process	mixed acid fermentation
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding
B	0051260	biological_process	protein homooligomerization
C	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
C	0005515	molecular_function	protein binding
C	0005829	cellular_component	cytosol
C	0006066	biological_process	alcohol metabolic process
C	0006115	biological_process	ethanol biosynthetic process
C	0006979	biological_process	response to oxidative stress
C	0008198	molecular_function	ferrous iron binding
C	0008774	molecular_function	acetaldehyde dehydrogenase (acetylating) activity
C	0015976	biological_process	carbon utilization
C	0016020	cellular_component	membrane
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
C	0019664	biological_process	mixed acid fermentation
C	0042802	molecular_function	identical protein binding
C	0046872	molecular_function	metal ion binding
C	0051260	biological_process	protein homooligomerization
D	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
D	0005515	molecular_function	protein binding
D	0005829	cellular_component	cytosol
D	0006066	biological_process	alcohol metabolic process
D	0006115	biological_process	ethanol biosynthetic process
D	0006979	biological_process	response to oxidative stress
D	0008198	molecular_function	ferrous iron binding
D	0008774	molecular_function	acetaldehyde dehydrogenase (acetylating) activity
D	0015976	biological_process	carbon utilization
D	0016020	cellular_component	membrane
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
D	0019664	biological_process	mixed acid fermentation
D	0042802	molecular_function	identical protein binding
D	0046872	molecular_function	metal ion binding
D	0051260	biological_process	protein homooligomerization
E	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
E	0005515	molecular_function	protein binding
E	0005829	cellular_component	cytosol
E	0006066	biological_process	alcohol metabolic process
E	0006115	biological_process	ethanol biosynthetic process
E	0006979	biological_process	response to oxidative stress
E	0008198	molecular_function	ferrous iron binding
E	0008774	molecular_function	acetaldehyde dehydrogenase (acetylating) activity
E	0015976	biological_process	carbon utilization
E	0016020	cellular_component	membrane
E	0016491	molecular_function	oxidoreductase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
E	0019664	biological_process	mixed acid fermentation
E	0042802	molecular_function	identical protein binding
E	0046872	molecular_function	metal ion binding
E	0051260	biological_process	protein homooligomerization
F	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
F	0005515	molecular_function	protein binding
F	0005829	cellular_component	cytosol
F	0006066	biological_process	alcohol metabolic process
F	0006115	biological_process	ethanol biosynthetic process
F	0006979	biological_process	response to oxidative stress
F	0008198	molecular_function	ferrous iron binding
F	0008774	molecular_function	acetaldehyde dehydrogenase (acetylating) activity
F	0015976	biological_process	carbon utilization
F	0016020	cellular_component	membrane
F	0016491	molecular_function	oxidoreductase activity
F	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
F	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
F	0019664	biological_process	mixed acid fermentation
F	0042802	molecular_function	identical protein binding
F	0046872	molecular_function	metal ion binding
F	0051260	biological_process	protein homooligomerization
G	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
G	0005515	molecular_function	protein binding
G	0005829	cellular_component	cytosol
G	0006066	biological_process	alcohol metabolic process
G	0006115	biological_process	ethanol biosynthetic process
G	0006979	biological_process	response to oxidative stress
G	0008198	molecular_function	ferrous iron binding
G	0008774	molecular_function	acetaldehyde dehydrogenase (acetylating) activity
G	0015976	biological_process	carbon utilization
G	0016020	cellular_component	membrane
G	0016491	molecular_function	oxidoreductase activity
G	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
G	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
G	0019664	biological_process	mixed acid fermentation
G	0042802	molecular_function	identical protein binding
G	0046872	molecular_function	metal ion binding
G	0051260	biological_process	protein homooligomerization
H	0004022	molecular_function	alcohol dehydrogenase (NAD+) activity
H	0005515	molecular_function	protein binding
H	0005829	cellular_component	cytosol
H	0006066	biological_process	alcohol metabolic process
H	0006115	biological_process	ethanol biosynthetic process
H	0006979	biological_process	response to oxidative stress
H	0008198	molecular_function	ferrous iron binding
H	0008774	molecular_function	acetaldehyde dehydrogenase (acetylating) activity
H	0015976	biological_process	carbon utilization
H	0016020	cellular_component	membrane
H	0016491	molecular_function	oxidoreductase activity
H	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
H	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
H	0019664	biological_process	mixed acid fermentation
H	0042802	molecular_function	identical protein binding
H	0046872	molecular_function	metal ion binding
H	0051260	biological_process	protein homooligomerization

Functional Information from PROSITE/UniProt

site_id	PS00060
Number of Residues	21
Details	ADH_IRON_2 Iron-containing alcohol dehydrogenases signature 2. GvCHsmAHkLGSqfhIpHGlA

Chain	Residue	Details
A	GLY720-ALA740

site_id	PS00913
Number of Residues	29
Details	ADH_IRON_1 Iron-containing alcohol dehydrogenases signature 1. AIvDanlvmdmPkslcAfGglDAVthamE

Chain	Residue	Details
A	ALA632-GLU660

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Nucleophile => ECO:0000250\|UniProtKB:Q9HTJ1

Chain	Residue	Details
A	CYS246
B	CYS246
C	CYS246
D	CYS246
E	CYS246
F	CYS246
G	CYS246
H	CYS246

site_id	SWS_FT_FI2
Number of Residues	80
Details	BINDING: BINDING => ECO:0000269\|PubMed:32523125, ECO:0007744\|PDB:7BVP

Chain	Residue	Details
A	ILE110
A	LYS619
B	ILE110
B	GLY195
B	GLY213
B	GLU335
B	LEU419
B	ASP487
B	ASP519
B	GLY546
B	VAL610
A	GLY195
B	LYS619
C	ILE110
C	GLY195
C	GLY213
C	GLU335
C	LEU419
C	ASP487
C	ASP519
C	GLY546
C	VAL610
A	GLY213
C	LYS619
D	ILE110
D	GLY195
D	GLY213
D	GLU335
D	LEU419
D	ASP487
D	ASP519
D	GLY546
D	VAL610
A	GLU335
D	LYS619
E	ILE110
E	GLY195
E	GLY213
E	GLU335
E	LEU419
E	ASP487
E	ASP519
E	GLY546
E	VAL610
A	LEU419
E	LYS619
F	ILE110
F	GLY195
F	GLY213
F	GLU335
F	LEU419
F	ASP487
F	ASP519
F	GLY546
F	VAL610
A	ASP487
F	LYS619
G	ILE110
G	GLY195
G	GLY213
G	GLU335
G	LEU419
G	ASP487
G	ASP519
G	GLY546
G	VAL610
A	ASP519
G	LYS619
H	ILE110
H	GLY195
H	GLY213
H	GLU335
H	LEU419
H	ASP487
H	ASP519
H	GLY546
H	VAL610
A	GLY546
H	LYS619
A	VAL610

site_id	SWS_FT_FI3
Number of Residues	32
Details	BINDING: BINDING => ECO:0000269\|PubMed:32188856, ECO:0000305\|PubMed:32523125, ECO:0007744\|PDB:6TQH, ECO:0007744\|PDB:6TQM

Chain	Residue	Details
A	ASP653
C	HIS657
C	HIS723
C	HIS737
D	ASP653
D	HIS657
D	HIS723
D	HIS737
E	ASP653
E	HIS657
E	HIS723
A	HIS657
E	HIS737
F	ASP653
F	HIS657
F	HIS723
F	HIS737
G	ASP653
G	HIS657
G	HIS723
G	HIS737
H	ASP653
A	HIS723
H	HIS657
H	HIS723
H	HIS737
A	HIS737
B	ASP653
B	HIS657
B	HIS723
B	HIS737
C	ASP653

site_id	SWS_FT_FI4
Number of Residues	8
Details	MOD_RES: N6-acetyllysine => ECO:0000269\|PubMed:18723842

Chain	Residue	Details
A	LYS358
B	LYS358
C	LYS358
D	LYS358
E	LYS358
F	LYS358
G	LYS358
H	LYS358

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)