6AFV

Proton pyrophosphatase-L555K mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004427	molecular_function	inorganic diphosphate phosphatase activity
A	0005774	cellular_component	vacuolar membrane
A	0006811	biological_process	monoatomic ion transport
A	0009678	molecular_function	diphosphate hydrolysis-driven proton transmembrane transporter activity
A	0016020	cellular_component	membrane
A	0046872	molecular_function	metal ion binding
A	1902600	biological_process	proton transmembrane transport
B	0004427	molecular_function	inorganic diphosphate phosphatase activity
B	0005774	cellular_component	vacuolar membrane
B	0006811	biological_process	monoatomic ion transport
B	0009678	molecular_function	diphosphate hydrolysis-driven proton transmembrane transporter activity
B	0016020	cellular_component	membrane
B	0046872	molecular_function	metal ion binding
B	1902600	biological_process	proton transmembrane transport

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	binding site for residue PO4 A 1001

Chain	Residue
A	LYS250
A	ASP731
A	PO41002
A	MG1005
A	MG1006
A	MG1007
A	HOH1136
A	HOH1176
A	ASP253
A	ASP283
A	ASP287
A	ASP507
A	ASN534
A	ASP691
A	ASP727
A	LYS730

---

site_id	AC2
Number of Residues	17
Details	binding site for residue PO4 A 1002

Chain	Residue
A	ASP253
A	ASP257
A	ASP507
A	ASP691
A	PO41001
A	MG1003
A	MG1004
A	MG1005
A	MG1006
A	MG1007
A	HOH1111
A	HOH1112
A	HOH1113
A	HOH1116
A	HOH1118
A	HOH1132
A	HOH1141

---

site_id	AC3
Number of Residues	8
Details	binding site for residue MG A 1003

Chain	Residue
A	ASP253
A	ASP257
A	ASP723
A	PO41002
A	MG1005
A	HOH1113
A	HOH1116
A	HOH1118

---

site_id	AC4
Number of Residues	7
Details	binding site for residue MG A 1004

Chain	Residue
A	ASP507
A	PO41002
A	MG1006
A	HOH1112
A	HOH1141
A	HOH1152
A	HOH1158

---

site_id	AC5
Number of Residues	9
Details	binding site for residue MG A 1005

Chain	Residue
A	LYS250
A	ASP253
A	ASP727
A	LYS730
A	PO41001
A	PO41002
A	MG1003
A	HOH1111
A	HOH1116

---

site_id	AC6
Number of Residues	7
Details	binding site for residue MG A 1006

Chain	Residue
A	ASP253
A	ASP283
A	ASP507
A	PO41001
A	PO41002
A	MG1004
A	HOH1112

---

site_id	AC7
Number of Residues	7
Details	binding site for residue MG A 1007

Chain	Residue
A	ASP530
A	ASN534
A	ASP691
A	PO41001
A	PO41002
A	K1008
A	HOH1132

---

site_id	AC8
Number of Residues	6
Details	binding site for residue K A 1008

Chain	Residue
A	GLY533
A	ASN534
A	MG1007
A	HOH1144
A	HOH1155
A	HOH1176

---

site_id	AC9
Number of Residues	4
Details	binding site for residue 1PG A 1009

Chain	Residue
A	MET592
A	LEU651
A	THR652
A	VAL656

---

site_id	AD1
Number of Residues	4
Details	binding site for residue 1PG A 1010

Chain	Residue
A	ILE577
A	LEU762
A	PHE766
B	1PG1011

---

site_id	AD2
Number of Residues	2
Details	binding site for residue 1PG A 1011

Chain	Residue
A	ASP636
A	LYS640

---

site_id	AD3
Number of Residues	14
Details	binding site for residue PO4 B 1001

Chain	Residue
B	MG1007
B	HOH1151
B	LYS250
B	ASP253
B	ASP287
B	ASP507
B	ASN534
B	ASP691
B	ASP727
B	LYS730
B	ASP731
B	PO41002
B	MG1005
B	MG1006

---

site_id	AD4
Number of Residues	14
Details	binding site for residue PO4 B 1002

Chain	Residue
B	ASP253
B	GLU268
B	ASP507
B	PO41001
B	MG1003
B	MG1004
B	MG1005
B	MG1006
B	MG1007
B	HOH1105
B	HOH1115
B	HOH1120
B	HOH1124
B	HOH1159

---

site_id	AD5
Number of Residues	7
Details	binding site for residue MG B 1003

Chain	Residue
B	ASP253
B	ASP257
B	PO41002
B	MG1005
B	HOH1105
B	HOH1115
B	HOH1128

---

site_id	AD6
Number of Residues	6
Details	binding site for residue MG B 1004

Chain	Residue
B	ASP507
B	PO41002
B	HOH1119
B	HOH1120
B	HOH1126
B	HOH1159

---

site_id	AD7
Number of Residues	8
Details	binding site for residue MG B 1005

Chain	Residue
B	ASP253
B	ASP727
B	LYS730
B	PO41001
B	PO41002
B	MG1003
B	HOH1115
B	HOH1124

---

site_id	AD8
Number of Residues	5
Details	binding site for residue MG B 1006

Chain	Residue
B	ASP283
B	ASP507
B	PO41001
B	PO41002
B	HOH1120

---

site_id	AD9
Number of Residues	6
Details	binding site for residue MG B 1007

Chain	Residue
B	ASP530
B	ASN534
B	ASP691
B	PO41001
B	PO41002
B	K1008

---

site_id	AE1
Number of Residues	4
Details	binding site for residue K B 1008

Chain	Residue
B	ASN534
B	MG1007
B	HOH1148
B	HOH1151

---

site_id	AE2
Number of Residues	4
Details	binding site for residue 1PG B 1009

Chain	Residue
B	TRP588
B	MET592
B	LEU648
B	THR652

---

site_id	AE3
Number of Residues	4
Details	binding site for residue 1PG B 1010

Chain	Residue
B	PHE660
B	LEU762
B	PHE766
B	HOH1108

---

site_id	AE4
Number of Residues	3
Details	binding site for residue 1PG B 1011

Chain	Residue
A	1PG1010
B	LYS457
B	PHE464

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	148
Details	Topological domain: {"description":"Intravacuolar","evidences":[{"source":"PubMed","id":"22456709","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	850
Details	Transmembrane: {"description":"Helical"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	370
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"source":"PubMed","id":"22456709","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"PubMed","id":"22456709","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	12
Details	Site: {"description":"Important for proton transport","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---