Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5ZRV

Structure of human mitochondrial trifunctional protein, octamer

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000062	molecular_function	fatty-acyl-CoA binding
A	0003824	molecular_function	catalytic activity
A	0003857	molecular_function	3-hydroxyacyl-CoA dehydrogenase activity
A	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
A	0003988	molecular_function	acetyl-CoA C-acyltransferase activity
A	0004300	molecular_function	enoyl-CoA hydratase activity
A	0005515	molecular_function	protein binding
A	0005739	cellular_component	mitochondrion
A	0005743	cellular_component	mitochondrial inner membrane
A	0006631	biological_process	fatty acid metabolic process
A	0006635	biological_process	fatty acid beta-oxidation
A	0009410	biological_process	response to xenobiotic stimulus
A	0016491	molecular_function	oxidoreductase activity
A	0016507	cellular_component	mitochondrial fatty acid beta-oxidation multienzyme complex
A	0016509	molecular_function	long-chain-3-hydroxyacyl-CoA dehydrogenase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0016740	molecular_function	transferase activity
A	0016829	molecular_function	lyase activity
A	0018812	molecular_function	3-hydroxyacyl-CoA dehydratase activity
A	0032868	biological_process	response to insulin
A	0035965	biological_process	cardiolipin acyl-chain remodeling
A	0042645	cellular_component	mitochondrial nucleoid
A	0044877	molecular_function	protein-containing complex binding
A	0051287	molecular_function	NAD binding
A	0070403	molecular_function	NAD+ binding
B	0003723	molecular_function	RNA binding
B	0003857	molecular_function	3-hydroxyacyl-CoA dehydrogenase activity
B	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
B	0003988	molecular_function	acetyl-CoA C-acyltransferase activity
B	0004300	molecular_function	enoyl-CoA hydratase activity
B	0005515	molecular_function	protein binding
B	0005739	cellular_component	mitochondrion
B	0005740	cellular_component	mitochondrial envelope
B	0005741	cellular_component	mitochondrial outer membrane
B	0005743	cellular_component	mitochondrial inner membrane
B	0005783	cellular_component	endoplasmic reticulum
B	0006631	biological_process	fatty acid metabolic process
B	0006635	biological_process	fatty acid beta-oxidation
B	0010467	biological_process	gene expression
B	0016507	cellular_component	mitochondrial fatty acid beta-oxidation multienzyme complex
B	0016746	molecular_function	acyltransferase activity
B	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
B	0042645	cellular_component	mitochondrial nucleoid
B	0050633	molecular_function	acetyl-CoA C-myristoyltransferase activity
B	0071222	biological_process	cellular response to lipopolysaccharide
B	0106222	molecular_function	lncRNA binding
C	0000062	molecular_function	fatty-acyl-CoA binding
C	0003824	molecular_function	catalytic activity
C	0003857	molecular_function	3-hydroxyacyl-CoA dehydrogenase activity
C	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
C	0003988	molecular_function	acetyl-CoA C-acyltransferase activity
C	0004300	molecular_function	enoyl-CoA hydratase activity
C	0005515	molecular_function	protein binding
C	0005739	cellular_component	mitochondrion
C	0005743	cellular_component	mitochondrial inner membrane
C	0006631	biological_process	fatty acid metabolic process
C	0006635	biological_process	fatty acid beta-oxidation
C	0009410	biological_process	response to xenobiotic stimulus
C	0016491	molecular_function	oxidoreductase activity
C	0016507	cellular_component	mitochondrial fatty acid beta-oxidation multienzyme complex
C	0016509	molecular_function	long-chain-3-hydroxyacyl-CoA dehydrogenase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0016740	molecular_function	transferase activity
C	0016829	molecular_function	lyase activity
C	0018812	molecular_function	3-hydroxyacyl-CoA dehydratase activity
C	0032868	biological_process	response to insulin
C	0035965	biological_process	cardiolipin acyl-chain remodeling
C	0042645	cellular_component	mitochondrial nucleoid
C	0044877	molecular_function	protein-containing complex binding
C	0051287	molecular_function	NAD binding
C	0070403	molecular_function	NAD+ binding
D	0003723	molecular_function	RNA binding
D	0003857	molecular_function	3-hydroxyacyl-CoA dehydrogenase activity
D	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
D	0003988	molecular_function	acetyl-CoA C-acyltransferase activity
D	0004300	molecular_function	enoyl-CoA hydratase activity
D	0005515	molecular_function	protein binding
D	0005739	cellular_component	mitochondrion
D	0005740	cellular_component	mitochondrial envelope
D	0005741	cellular_component	mitochondrial outer membrane
D	0005743	cellular_component	mitochondrial inner membrane
D	0005783	cellular_component	endoplasmic reticulum
D	0006631	biological_process	fatty acid metabolic process
D	0006635	biological_process	fatty acid beta-oxidation
D	0010467	biological_process	gene expression
D	0016507	cellular_component	mitochondrial fatty acid beta-oxidation multienzyme complex
D	0016746	molecular_function	acyltransferase activity
D	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
D	0042645	cellular_component	mitochondrial nucleoid
D	0050633	molecular_function	acetyl-CoA C-myristoyltransferase activity
D	0071222	biological_process	cellular response to lipopolysaccharide
D	0106222	molecular_function	lncRNA binding
E	0000062	molecular_function	fatty-acyl-CoA binding
E	0003824	molecular_function	catalytic activity
E	0003857	molecular_function	3-hydroxyacyl-CoA dehydrogenase activity
E	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
E	0003988	molecular_function	acetyl-CoA C-acyltransferase activity
E	0004300	molecular_function	enoyl-CoA hydratase activity
E	0005515	molecular_function	protein binding
E	0005739	cellular_component	mitochondrion
E	0005743	cellular_component	mitochondrial inner membrane
E	0006631	biological_process	fatty acid metabolic process
E	0006635	biological_process	fatty acid beta-oxidation
E	0009410	biological_process	response to xenobiotic stimulus
E	0016491	molecular_function	oxidoreductase activity
E	0016507	cellular_component	mitochondrial fatty acid beta-oxidation multienzyme complex
E	0016509	molecular_function	long-chain-3-hydroxyacyl-CoA dehydrogenase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0016740	molecular_function	transferase activity
E	0016829	molecular_function	lyase activity
E	0018812	molecular_function	3-hydroxyacyl-CoA dehydratase activity
E	0032868	biological_process	response to insulin
E	0035965	biological_process	cardiolipin acyl-chain remodeling
E	0042645	cellular_component	mitochondrial nucleoid
E	0044877	molecular_function	protein-containing complex binding
E	0051287	molecular_function	NAD binding
E	0070403	molecular_function	NAD+ binding
F	0003723	molecular_function	RNA binding
F	0003857	molecular_function	3-hydroxyacyl-CoA dehydrogenase activity
F	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
F	0003988	molecular_function	acetyl-CoA C-acyltransferase activity
F	0004300	molecular_function	enoyl-CoA hydratase activity
F	0005515	molecular_function	protein binding
F	0005739	cellular_component	mitochondrion
F	0005740	cellular_component	mitochondrial envelope
F	0005741	cellular_component	mitochondrial outer membrane
F	0005743	cellular_component	mitochondrial inner membrane
F	0005783	cellular_component	endoplasmic reticulum
F	0006631	biological_process	fatty acid metabolic process
F	0006635	biological_process	fatty acid beta-oxidation
F	0010467	biological_process	gene expression
F	0016507	cellular_component	mitochondrial fatty acid beta-oxidation multienzyme complex
F	0016746	molecular_function	acyltransferase activity
F	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
F	0042645	cellular_component	mitochondrial nucleoid
F	0050633	molecular_function	acetyl-CoA C-myristoyltransferase activity
F	0071222	biological_process	cellular response to lipopolysaccharide
F	0106222	molecular_function	lncRNA binding
G	0000062	molecular_function	fatty-acyl-CoA binding
G	0003824	molecular_function	catalytic activity
G	0003857	molecular_function	3-hydroxyacyl-CoA dehydrogenase activity
G	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
G	0003988	molecular_function	acetyl-CoA C-acyltransferase activity
G	0004300	molecular_function	enoyl-CoA hydratase activity
G	0005515	molecular_function	protein binding
G	0005739	cellular_component	mitochondrion
G	0005743	cellular_component	mitochondrial inner membrane
G	0006631	biological_process	fatty acid metabolic process
G	0006635	biological_process	fatty acid beta-oxidation
G	0009410	biological_process	response to xenobiotic stimulus
G	0016491	molecular_function	oxidoreductase activity
G	0016507	cellular_component	mitochondrial fatty acid beta-oxidation multienzyme complex
G	0016509	molecular_function	long-chain-3-hydroxyacyl-CoA dehydrogenase activity
G	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
G	0016740	molecular_function	transferase activity
G	0016829	molecular_function	lyase activity
G	0018812	molecular_function	3-hydroxyacyl-CoA dehydratase activity
G	0032868	biological_process	response to insulin
G	0035965	biological_process	cardiolipin acyl-chain remodeling
G	0042645	cellular_component	mitochondrial nucleoid
G	0044877	molecular_function	protein-containing complex binding
G	0051287	molecular_function	NAD binding
G	0070403	molecular_function	NAD+ binding
H	0003723	molecular_function	RNA binding
H	0003857	molecular_function	3-hydroxyacyl-CoA dehydrogenase activity
H	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
H	0003988	molecular_function	acetyl-CoA C-acyltransferase activity
H	0004300	molecular_function	enoyl-CoA hydratase activity
H	0005515	molecular_function	protein binding
H	0005739	cellular_component	mitochondrion
H	0005740	cellular_component	mitochondrial envelope
H	0005741	cellular_component	mitochondrial outer membrane
H	0005743	cellular_component	mitochondrial inner membrane
H	0005783	cellular_component	endoplasmic reticulum
H	0006631	biological_process	fatty acid metabolic process
H	0006635	biological_process	fatty acid beta-oxidation
H	0010467	biological_process	gene expression
H	0016507	cellular_component	mitochondrial fatty acid beta-oxidation multienzyme complex
H	0016746	molecular_function	acyltransferase activity
H	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
H	0042645	cellular_component	mitochondrial nucleoid
H	0050633	molecular_function	acetyl-CoA C-myristoyltransferase activity
H	0071222	biological_process	cellular response to lipopolysaccharide
H	0106222	molecular_function	lncRNA binding

Functional Information from PROSITE/UniProt

site_id	PS00067
Number of Residues	25
Details	3HCDH 3-hydroxyacyl-CoA dehydrogenase signature. DgpGFYtTRclaPMMsevir.ILqeG

Chain	Residue	Details
A	ASP541-GLY565

site_id	PS00098
Number of Residues	19
Details	THIOLASE_1 Thiolases acyl-enzyme intermediate signature. VTMaCISANqAMttgvglI

Chain	Residue	Details
B	VAL134-ILE152

site_id	PS00099
Number of Residues	14
Details	THIOLASE_3 Thiolases active site. GLVAACAAgGqGhA

Chain	Residue	Details
B	GLY453-ALA466

site_id	PS00166
Number of Residues	21
Details	ENOYL_COA_HYDRATASE Enoyl-CoA hydratase/isomerase signature. VAaINGsclGGGlevaIsCQY

Chain	Residue	Details
A	VAL138-TYR158

site_id	PS00737
Number of Residues	17
Details	THIOLASE_2 Thiolases signature 2. NnwGGsLSlGHPfGaTG

Chain	Residue	Details
B	ASN418-GLY434

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	188
Details	INTRAMEM: INTRAMEM => ECO:0000305\|PubMed:29915090

Chain	Residue	Details
B	ILE173-SER220
D	ILE173-SER220
F	ILE173-SER220
H	ILE173-SER220

site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: Acyl-thioester intermediate => ECO:0000305\|PubMed:30850536

Chain	Residue	Details
B	CYS138
D	CYS138
F	CYS138
H	CYS138
E	GLU151
E	GLU173
G	GLU151
G	GLU173

site_id	SWS_FT_FI3
Number of Residues	4
Details	ACT_SITE: Proton donor/acceptor => ECO:0000305\|PubMed:30850536

Chain	Residue	Details
B	CYS458
D	CYS458
F	CYS458
H	CYS458

site_id	SWS_FT_FI4
Number of Residues	4
Details	SITE: Increases nucleophilicity of active site Cys => ECO:0000305\|PubMed:30850536

Chain	Residue	Details
B	HIS428
A	LYS406
A	LYS411
A	LYS460
A	LYS505
A	LYS519
A	LYS569
A	LYS644
A	LYS664
A	LYS728
A	LYS759
D	HIS428
C	LYS46
C	LYS60
C	LYS166
C	LYS214
C	LYS249
C	LYS303
C	LYS326
C	LYS334
C	LYS350
C	LYS406
F	HIS428
C	LYS411
C	LYS460
C	LYS505
C	LYS519
C	LYS569
C	LYS644
C	LYS664
C	LYS728
C	LYS759
E	LYS46
H	HIS428
E	LYS60
E	LYS166
E	LYS214
E	LYS249
E	LYS303
E	LYS326
E	LYS334
E	LYS350
E	LYS406
E	LYS411
A	LYS249
E	LYS460
E	LYS505
E	LYS519
E	LYS569
E	LYS644
E	LYS664
E	LYS728
E	LYS759
G	LYS46
G	LYS60
A	LYS303
G	LYS166
G	LYS214
G	LYS249
G	LYS303
G	LYS326
G	LYS334
G	LYS350
G	LYS406
G	LYS411
G	LYS460
A	LYS326
G	LYS505
G	LYS519
G	LYS569
G	LYS644
G	LYS664
G	LYS728
G	LYS759
A	LYS334
A	LYS350

site_id	SWS_FT_FI5
Number of Residues	16
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250\|UniProtKB:Q99JY0

Chain	Residue	Details
B	LYS72
F	LYS188
F	LYS293
F	LYS332
H	LYS72
H	LYS188
H	LYS293
H	LYS332
B	LYS188
B	LYS293
B	LYS332
D	LYS72
D	LYS188
D	LYS293
D	LYS332
F	LYS72

site_id	SWS_FT_FI6
Number of Residues	12
Details	MOD_RES: N6-succinyllysine => ECO:0000250\|UniProtKB:Q99JY0

Chain	Residue	Details
B	LYS190
H	LYS190
H	LYS272
H	LYS291
E	LYS213
E	LYS230
E	LYS415
E	LYS440
E	LYS634
E	LYS646
G	LYS213
B	LYS272
G	LYS230
G	LYS415
G	LYS440
G	LYS634
G	LYS646
B	LYS291
D	LYS190
D	LYS272
D	LYS291
F	LYS190
F	LYS272
F	LYS291

site_id	SWS_FT_FI7
Number of Residues	12
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:Q99JY0

Chain	Residue	Details
B	LYS298
H	LYS298
H	LYS348
H	LYS361
B	LYS348
B	LYS361
D	LYS298
D	LYS348
D	LYS361
F	LYS298
F	LYS348
F	LYS361

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:Q8BMS1

Chain	Residue	Details
A	SER316
C	SER316
E	SER316
G	SER316

site_id	SWS_FT_FI9
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:24275569

Chain	Residue	Details
A	THR395
C	THR395
E	THR395
G	THR395

site_id	SWS_FT_FI10
Number of Residues	4
Details	MOD_RES: Omega-N-methylarginine => ECO:0000250\|UniProtKB:Q8BMS1

Chain	Residue	Details
A	ARG399
C	ARG399
E	ARG399
G	ARG399

site_id	SWS_FT_FI11
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:Q64428

Chain	Residue	Details
A	SER419
A	SER650
C	SER419
C	SER650
E	SER419
E	SER650
G	SER419
G	SER650

site_id	SWS_FT_FI12
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
A	SER756
C	SER756
E	SER756
G	SER756

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)