5ZE1

Hairpin Forming Complex, RAG1/2-Nicked 12RSS/23RSS complex in 2mM Mn2+ for 10 min at 4'C

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004519	molecular_function	endonuclease activity
A	0033151	biological_process	V(D)J recombination
A	0043565	molecular_function	sequence-specific DNA binding
A	0061630	molecular_function	ubiquitin protein ligase activity
B	0003677	molecular_function	DNA binding
B	0005634	cellular_component	nucleus
B	0006310	biological_process	DNA recombination
C	0004519	molecular_function	endonuclease activity
C	0033151	biological_process	V(D)J recombination
C	0043565	molecular_function	sequence-specific DNA binding
C	0061630	molecular_function	ubiquitin protein ligase activity
D	0003677	molecular_function	DNA binding
D	0005634	cellular_component	nucleus
D	0006310	biological_process	DNA recombination
N	0003677	molecular_function	DNA binding
N	0005634	cellular_component	nucleus

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN A 2001

Chain	Residue
A	CYS727
A	CYS730
A	HIS937
A	HIS942

---

site_id	AC2
Number of Residues	5
Details	binding site for residue MN A 2002

Chain	Residue
A	ASP600
A	GLU962
A	MN2003
F	DG30
F	DT31

---

site_id	AC3
Number of Residues	7
Details	binding site for residue MN A 2003

Chain	Residue
A	ASP600
A	ASP708
A	MN2002
A	HOH2104
F	DT31
I	DA16
I	HOH101

---

site_id	AC4
Number of Residues	6
Details	binding site for residue EDO A 2004

Chain	Residue
A	THR728
A	ARG748
A	ASN753
A	THR787
A	VAL788
A	PRO789

---

site_id	AC5
Number of Residues	5
Details	binding site for residue K A 2005

Chain	Residue
A	GLU649
A	SER963
A	HOH2101
L	DA18
L	DC19

---

site_id	AC6
Number of Residues	4
Details	binding site for residue ZN C 2001

Chain	Residue
C	CYS727
C	CYS730
C	HIS937
C	HIS942

---

site_id	AC7
Number of Residues	6
Details	binding site for residue MN C 2002

Chain	Residue
C	ASP600
C	ASP708
C	HOH2101
C	HOH2106
G	DT42
J	DA16

---

site_id	AC8
Number of Residues	5
Details	binding site for residue MN C 2003

Chain	Residue
C	ASP600
C	GLU962
G	DG41
G	DT42
G	HOH103

---

site_id	AC9
Number of Residues	6
Details	binding site for residue K C 2004

Chain	Residue
C	GLU649
C	SER963
C	HOH2104
G	HOH102
M	DA18
M	DC19

---

Functional Information from PROSITE/UniProt

site_id	PS00353
Number of Residues	12
Details	HMG_BOX_1 HMG box A DNA-binding domain signature. FSKKCsERWKTM

Chain	Residue	Details
N	PHE41-MET52

---

site_id	PS00435
Number of Residues	11
Details	PEROXIDASE_1 Peroxidases proximal heme-ligand signature. DTVYILGGHSL

Chain	Residue	Details
B	ASP214-LEU224

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Site: {"description":"Essential for DNA hairpin formation, participates in base-stacking interactions near the cleavage site"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	67
Details	DNA binding: {"description":"NBD","evidences":[{"source":"PROSITE-ProRule","id":"PRU00820","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	16
Details	Region: {"description":"NLS 1","evidences":[{"source":"UniProtKB","id":"P63159","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	16
Details	Motif: {"description":"Nuclear localization signal (NLS) 1","evidences":[{"source":"UniProtKB","id":"P63159","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Site: {"description":"Cleavage; by CASP1","evidences":[{"source":"UniProtKB","id":"P09429","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	5
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P10103","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Modified residue: {"description":"Cysteine sulfonic acid (-SO3H); alternate","evidences":[{"source":"UniProtKB","id":"P63159","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	3
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"23806337","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P09429","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	1
Details	Modified residue: {"description":"Cysteine sulfonic acid (-SO3H)","evidences":[{"source":"UniProtKB","id":"P63159","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	5
Details	Cross-link: {"description":"Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-?)","evidences":[{"source":"UniProtKB","id":"P09429","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---