5XWP
Crystal structure of LbuCas13a-crRNA-target RNA ternary complex
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003723 | molecular_function | RNA binding |
| A | 0004518 | molecular_function | nuclease activity |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0051607 | biological_process | defense response to virus |
| B | 0003723 | molecular_function | RNA binding |
| B | 0004518 | molecular_function | nuclease activity |
| B | 0004519 | molecular_function | endonuclease activity |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0051607 | biological_process | defense response to virus |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 104 |
| Details | Region: {"description":"Binds crRNA repeat","evidences":[{"source":"PubMed","id":"28757251","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 38 |
| Details | Region: {"description":"Binds crRNA processing site","evidences":[{"source":"PubMed","id":"28757251","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 6 |
| Details | Region: {"description":"Binds target RNA","evidences":[{"source":"PubMed","id":"28757251","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 62 |
| Details | Region: {"description":"Binds crRNA spacer","evidences":[{"source":"PubMed","id":"28757251","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 12 |
| Details | Region: {"description":"Binds crRNA spacer and target RNA","evidences":[{"source":"PubMed","id":"28757251","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 6 |
| Details | Region: {"description":"Binds 3'-end of target RNA, in adjacent protein","evidences":[{"source":"PubMed","id":"28757251","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 8 |
| Details | Active site: {"description":"For target RNA cleavage","evidences":[{"source":"PubMed","id":"27669025","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 7 |
| Details | Site: {"description":"Binds target RNA","evidences":[{"source":"PubMed","id":"28757251","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | Site: {"description":"Binds 3' nucleotide of the target RNA PFS","evidences":[{"source":"PubMed","id":"28757251","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 4 |
| Details | Site: {"description":"Binds crRNA repeat","evidences":[{"source":"PubMed","id":"28757251","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 2 |
| Details | Site: {"description":"Binds 3'-end of target RNA, in adjacent protein","evidences":[{"source":"PubMed","id":"28757251","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 5 |
| Details | Site: {"description":"Binds crRNA spacer","evidences":[{"source":"PubMed","id":"28757251","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
