5XTC

Cryo-EM structure of human respiratory complex I transmembrane arm

Functional Information from GO Data

Chain	GOid	namespace	contents
S	0005739	cellular_component	mitochondrion
S	0005743	cellular_component	mitochondrial inner membrane
S	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
S	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
S	0009060	biological_process	aerobic respiration
S	0031966	cellular_component	mitochondrial membrane
S	0032981	biological_process	mitochondrial respiratory chain complex I assembly
S	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
S	0045271	cellular_component	respiratory chain complex I
S	1902600	biological_process	proton transmembrane transport
U	0005515	molecular_function	protein binding
U	0005739	cellular_component	mitochondrion
U	0005743	cellular_component	mitochondrial inner membrane
U	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
U	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
U	0009060	biological_process	aerobic respiration
U	0032981	biological_process	mitochondrial respiratory chain complex I assembly
U	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
U	0045271	cellular_component	respiratory chain complex I
U	1902600	biological_process	proton transmembrane transport
V	0005515	molecular_function	protein binding
V	0005739	cellular_component	mitochondrion
V	0005743	cellular_component	mitochondrial inner membrane
V	0009060	biological_process	aerobic respiration
V	0032981	biological_process	mitochondrial respiratory chain complex I assembly
V	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
V	0045271	cellular_component	respiratory chain complex I
X	0006633	biological_process	fatty acid biosynthetic process
Y	0005743	cellular_component	mitochondrial inner membrane
Y	0045271	cellular_component	respiratory chain complex I
Z	0005739	cellular_component	mitochondrion
Z	0022900	biological_process	electron transport chain
b	0005515	molecular_function	protein binding
b	0005654	cellular_component	nucleoplasm
b	0005739	cellular_component	mitochondrion
b	0005743	cellular_component	mitochondrial inner membrane
b	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
b	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
b	0009060	biological_process	aerobic respiration
b	0031966	cellular_component	mitochondrial membrane
b	0032981	biological_process	mitochondrial respiratory chain complex I assembly
b	0042775	biological_process	mitochondrial ATP synthesis coupled electron transport
b	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
b	0045271	cellular_component	respiratory chain complex I
b	1902600	biological_process	proton transmembrane transport
c	0005739	cellular_component	mitochondrion
c	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
d	0005515	molecular_function	protein binding
d	0005739	cellular_component	mitochondrion
d	0005743	cellular_component	mitochondrial inner membrane
d	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
d	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
d	0009060	biological_process	aerobic respiration
d	0032981	biological_process	mitochondrial respiratory chain complex I assembly
d	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
d	0045271	cellular_component	respiratory chain complex I
d	1902600	biological_process	proton transmembrane transport
f	0005739	cellular_component	mitochondrion
f	0045271	cellular_component	respiratory chain complex I
g	0005515	molecular_function	protein binding
g	0005737	cellular_component	cytoplasm
g	0005739	cellular_component	mitochondrion
g	0005743	cellular_component	mitochondrial inner membrane
g	0005886	cellular_component	plasma membrane
g	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
g	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
g	0009060	biological_process	aerobic respiration
g	0010918	biological_process	positive regulation of mitochondrial membrane potential
g	0032981	biological_process	mitochondrial respiratory chain complex I assembly
g	0035577	cellular_component	azurophil granule membrane
g	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
g	0045271	cellular_component	respiratory chain complex I
g	1901223	biological_process	negative regulation of non-canonical NF-kappaB signal transduction
g	1902600	biological_process	proton transmembrane transport
g	1903427	biological_process	negative regulation of reactive oxygen species biosynthetic process
g	2001171	biological_process	positive regulation of ATP biosynthetic process
h	0005515	molecular_function	protein binding
h	0005739	cellular_component	mitochondrion
h	0005743	cellular_component	mitochondrial inner membrane
h	0005758	cellular_component	mitochondrial intermembrane space
h	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
h	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
h	0009060	biological_process	aerobic respiration
h	0032981	biological_process	mitochondrial respiratory chain complex I assembly
h	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
h	0045271	cellular_component	respiratory chain complex I
h	1902600	biological_process	proton transmembrane transport
i	0001666	biological_process	response to hypoxia
i	0005515	molecular_function	protein binding
i	0005739	cellular_component	mitochondrion
i	0005743	cellular_component	mitochondrial inner membrane
i	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
i	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
i	0009060	biological_process	aerobic respiration
i	0019901	molecular_function	protein kinase binding
i	0032981	biological_process	mitochondrial respiratory chain complex I assembly
i	0035255	molecular_function	ionotropic glutamate receptor binding
i	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
i	0045271	cellular_component	respiratory chain complex I
i	0072593	biological_process	reactive oxygen species metabolic process
i	1902600	biological_process	proton transmembrane transport
j	0005515	molecular_function	protein binding
j	0005739	cellular_component	mitochondrion
j	0005743	cellular_component	mitochondrial inner membrane
j	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
j	0006979	biological_process	response to oxidative stress
j	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
j	0009060	biological_process	aerobic respiration
j	0009642	biological_process	response to light intensity
j	0009725	biological_process	response to hormone
j	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
j	0045271	cellular_component	respiratory chain complex I
j	0071385	biological_process	cellular response to glucocorticoid stimulus
j	1902600	biological_process	proton transmembrane transport
k	0005739	cellular_component	mitochondrion
k	0005743	cellular_component	mitochondrial inner membrane
k	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
k	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
k	0009060	biological_process	aerobic respiration
k	0016651	molecular_function	oxidoreductase activity, acting on NAD(P)H
k	0042773	biological_process	ATP synthesis coupled electron transport
k	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
k	0045271	cellular_component	respiratory chain complex I
k	1902600	biological_process	proton transmembrane transport
l	0001666	biological_process	response to hypoxia
l	0005739	cellular_component	mitochondrion
l	0005743	cellular_component	mitochondrial inner membrane
l	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
l	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
l	0009060	biological_process	aerobic respiration
l	0015990	biological_process	electron transport coupled proton transport
l	0032981	biological_process	mitochondrial respiratory chain complex I assembly
l	0042542	biological_process	response to hydrogen peroxide
l	0042773	biological_process	ATP synthesis coupled electron transport
l	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
l	0045271	cellular_component	respiratory chain complex I
m	0005739	cellular_component	mitochondrion
m	0005743	cellular_component	mitochondrial inner membrane
m	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
m	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
m	0009060	biological_process	aerobic respiration
m	0032981	biological_process	mitochondrial respiratory chain complex I assembly
m	0035094	biological_process	response to nicotine
m	0042220	biological_process	response to cocaine
m	0042542	biological_process	response to hydrogen peroxide
m	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
m	0045271	cellular_component	respiratory chain complex I
m	1902600	biological_process	proton transmembrane transport
n	0005739	cellular_component	mitochondrion
n	0005743	cellular_component	mitochondrial inner membrane
n	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
n	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
n	0009060	biological_process	aerobic respiration
n	0016607	cellular_component	nuclear speck
n	0032981	biological_process	mitochondrial respiratory chain complex I assembly
n	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
n	0045271	cellular_component	respiratory chain complex I
n	1902600	biological_process	proton transmembrane transport
o	0005654	cellular_component	nucleoplasm
o	0005739	cellular_component	mitochondrion
o	0005743	cellular_component	mitochondrial inner membrane
o	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
o	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
o	0009060	biological_process	aerobic respiration
o	0032981	biological_process	mitochondrial respiratory chain complex I assembly
o	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
o	0045271	cellular_component	respiratory chain complex I
o	1902600	biological_process	proton transmembrane transport
p	0005515	molecular_function	protein binding
p	0005739	cellular_component	mitochondrion
p	0005743	cellular_component	mitochondrial inner membrane
p	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
p	0007605	biological_process	sensory perception of sound
p	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
p	0009060	biological_process	aerobic respiration
p	0032981	biological_process	mitochondrial respiratory chain complex I assembly
p	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
p	0045271	cellular_component	respiratory chain complex I
p	1902600	biological_process	proton transmembrane transport
r	0001666	biological_process	response to hypoxia
r	0001701	biological_process	in utero embryonic development
r	0003954	molecular_function	NADH dehydrogenase activity
r	0005739	cellular_component	mitochondrion
r	0005743	cellular_component	mitochondrial inner membrane
r	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
r	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
r	0009060	biological_process	aerobic respiration
r	0015990	biological_process	electron transport coupled proton transport
r	0021549	biological_process	cerebellum development
r	0032981	biological_process	mitochondrial respiratory chain complex I assembly
r	0035094	biological_process	response to nicotine
r	0042773	biological_process	ATP synthesis coupled electron transport
r	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
r	0045271	cellular_component	respiratory chain complex I
r	0045471	biological_process	response to ethanol
r	0048039	molecular_function	ubiquinone binding
s	0001666	biological_process	response to hypoxia
s	0003954	molecular_function	NADH dehydrogenase activity
s	0005515	molecular_function	protein binding
s	0005739	cellular_component	mitochondrion
s	0005743	cellular_component	mitochondrial inner membrane
s	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
s	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
s	0009060	biological_process	aerobic respiration
s	0009410	biological_process	response to xenobiotic stimulus
s	0014070	biological_process	response to organic cyclic compound
s	0016020	cellular_component	membrane
s	0030425	cellular_component	dendrite
s	0031966	cellular_component	mitochondrial membrane
s	0032981	biological_process	mitochondrial respiratory chain complex I assembly
s	0033194	biological_process	response to hydroperoxide
s	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
s	0043025	cellular_component	neuronal cell body
s	0045271	cellular_component	respiratory chain complex I
s	1902600	biological_process	proton transmembrane transport
u	0005515	molecular_function	protein binding
u	0005739	cellular_component	mitochondrion
u	0005743	cellular_component	mitochondrial inner membrane
u	0005758	cellular_component	mitochondrial intermembrane space
u	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
u	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
u	0009060	biological_process	aerobic respiration
u	0032981	biological_process	mitochondrial respiratory chain complex I assembly
u	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
u	0044877	molecular_function	protein-containing complex binding
u	0045271	cellular_component	respiratory chain complex I
u	1902600	biological_process	proton transmembrane transport
v	0005739	cellular_component	mitochondrion
w	0005737	cellular_component	cytoplasm
w	0005739	cellular_component	mitochondrion
w	0005743	cellular_component	mitochondrial inner membrane
w	0005759	cellular_component	mitochondrial matrix
w	0006120	biological_process	mitochondrial electron transport, NADH to ubiquinone
w	0008137	molecular_function	NADH dehydrogenase (ubiquinone) activity
w	0009060	biological_process	aerobic respiration
w	0032981	biological_process	mitochondrial respiratory chain complex I assembly
w	0042776	biological_process	proton motive force-driven mitochondrial ATP synthesis
w	0045271	cellular_component	respiratory chain complex I
w	1902600	biological_process	proton transmembrane transport

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	binding site for residue PLX U 101

Chain	Residue
U	ALA12
U	GLU16
U	PHE23
U	GLY26
j	TRP106
j	LEU107
j	ASP112
s	PRO295

---

site_id	AC2
Number of Residues	11
Details	binding site for residue CDL V 201

Chain	Residue
V	TYR39
V	ARG40
V	ASN44
V	TYR59
V	THR62
i	PRO110
i	HIS112
i	LEU160
l	ILE576
l	THR577
V	THR35

---

site_id	AC3
Number of Residues	5
Details	binding site for residue PEE V 202

Chain	Residue
V	TRP133
i	LEU276
i	ILE277
i	THR284
l	PEE701

---

site_id	AC4
Number of Residues	7
Details	binding site for residue PLX V 203

Chain	Residue
V	TYR108
V	GLY109
V	ALA112
l	LEU553
o	PRO82
o	ALA91
o	PHE95

---

site_id	AC5
Number of Residues	12
Details	binding site for residue PEE W 201

Chain	Residue
S	HIS40
W	PHE140
W	MET141
W	TYR143
W	THR144
j	LEU7
m	VAL37
m	LEU44
m	PHE56
s	LEU77
s	LEU98
s	PHE104

---

site_id	AC6
Number of Residues	9
Details	binding site for residue PLX b 201

Chain	Residue
a	THR87
a	ASN90
a	GLN95
b	TYR84
b	TYR88
b	GLU92
d	ARG43
e	TYR108
r	PLX502

---

site_id	AC7
Number of Residues	10
Details	binding site for residue PLX g 201

Chain	Residue
g	PRO9
g	LEU10
g	PHE12
g	ARG77
h	PRO2
h	PHE3
i	THR132
i	TRP133
i	TYR143
i	ILE346

---

site_id	AC8
Number of Residues	4
Details	binding site for residue PLX g 202

Chain	Residue
f	HIS62
g	LEU25
g	PHE35
g	PHE68

---

site_id	AC9
Number of Residues	10
Details	binding site for residue PLX g 203

Chain	Residue
f	LYS43
f	THR47
g	ARG48
g	ARG50
g	ARG59
g	LEU62
g	TYR63
g	PHE67
i	PRO324
i	PHE325

---

site_id	AD1
Number of Residues	9
Details	binding site for residue CDL i 401

Chain	Residue
g	LEU46
i	THR238
i	TRP239
i	THR241
i	PRO242
i	LEU243
r	TRP16
r	SER90
r	LYS93

---

site_id	AD2
Number of Residues	11
Details	binding site for residue PEE l 701

Chain	Residue
Q	GLN46
Q	TYR53
V	PEE202
i	ILE285
i	LEU288
i	TYR291
i	ARG295
l	SER567
l	GLN570
l	ILE571
r	VAL155

---

site_id	AD3
Number of Residues	11
Details	binding site for residue PEE l 702

Chain	Residue
l	LEU173
l	TYR174
l	MET533
l	HIS534
l	ILE537
l	PRO538
l	GLY541
l	THR544
c	ARG116
l	THR166
l	GLN170

---

site_id	AD4
Number of Residues	13
Details	binding site for residue CDL l 703

Chain	Residue
a	LEU68
a	LEU71
a	ARG72
l	THR22
l	LEU23
l	PRO26
l	LYS28
l	GLN116
l	LYS119
l	TYR120
r	LEU354
r	PHE357
r	LEU360

---

site_id	AD5
Number of Residues	6
Details	binding site for residue CDL l 704

Chain	Residue
c	ASP148
l	SER209
l	THR211
l	LEU214
l	LEU272
r	ASN390

---

site_id	AD6
Number of Residues	11
Details	binding site for residue CDL n 101

Chain	Residue
g	THR26
g	LEU31
g	TYR32
i	PRO338
n	TYR26
n	LYS30
n	ARG34
r	THR55
r	PHE121
u	PHE169
u	TRP170

---

site_id	AD7
Number of Residues	15
Details	binding site for residue 8Q1 p 201

Chain	Residue
X	ASP111
X	SER112
X	LEU113
p	GLN13
p	ALA23
p	HIS26
p	LEU27
p	MET44
p	ARG47
p	PHE48
p	HIS51
p	LYS52
p	GLU54
p	ALA60
p	ARG74

---

site_id	AD8
Number of Residues	7
Details	binding site for residue PLX r 501

Chain	Residue
o	TYR102
r	ASN188
r	TRP190
r	ASN193
r	HIS256
r	MET257
r	PRO260

---

site_id	AD9
Number of Residues	8
Details	binding site for residue PLX r 502

Chain	Residue
b	PLX201
e	ALA107
e	TYR108
l	ILE16
r	LEU68
r	TRP71
r	LEU447
r	ASN450

---

site_id	AE1
Number of Residues	7
Details	binding site for residue PLX s 401

Chain	Residue
s	MET183
s	MET184
s	ILE273
s	TYR277
s	PHE280
s	LEU288
s	ASN292

---

Functional Information from PROSITE/UniProt

site_id	PS00012
Number of Residues	16
Details	PHOSPHOPANTETHEINE Phosphopantetheine attachment site. DLGLDSLDQVEIIMAM

Chain	Residue	Details
X	ASP107-MET122

---

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DIDAEKLMCpqEI

Chain	Residue	Details
X	ASP132-ILE144

---

site_id	PS00667
Number of Residues	16
Details	COMPLEX1_ND1_1 Respiratory-chain NADH dehydrogenase subunit 1 signature 1. GLLQpFaDAMKLFtKE

Chain	Residue	Details
s	GLY44-GLU59

---

site_id	PS00668
Number of Residues	14
Details	COMPLEX1_ND1_2 Respiratory-chain NADH dehydrogenase subunit 1 signature 2. PFDLAEGEseLVs.G

Chain	Residue	Details
s	PRO197-GLY210

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	160
Details	TRANSMEM: Helical => ECO:0000255

Chain	Residue	Details
s	PRO2-LEU22
r	LEU351-PRO371
r	ILE391-PHE411
l	TRP407-LEU429
l	ALA458-PHE478
l	ILE482-LEU502
l	GLY582-ILE602
s	ILE68-PRO88
s	LEU100-GLY120
s	LEU146-ILE166
s	HIS171-ALA191
s	ILE231-SER251
s	GLU253-ILE273
s	LEU294-ILE314
r	SER308-ALA330

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:Q99LC3

Chain	Residue	Details
w	LYS285
Z	LYS34

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
o	SER26

---