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5XMI

Cryo-EM Structure of the ATP-bound VPS4 mutant-E233Q hexamer (masked)

Functional Information from GO Data
ChainGOidnamespacecontents
A0001778biological_processplasma membrane repair
A0005515molecular_functionprotein binding
A0005524molecular_functionATP binding
A0005643cellular_componentnuclear pore
A0005737cellular_componentcytoplasm
A0005768cellular_componentendosome
A0005783cellular_componentendoplasmic reticulum
A0005886cellular_componentplasma membrane
A0006914biological_processautophagy
A0006997biological_processnucleus organization
A0007033biological_processvacuole organization
A0010008cellular_componentendosome membrane
A0015031biological_processprotein transport
A0016020cellular_componentmembrane
A0016125biological_processsterol metabolic process
A0016197biological_processendosomal transport
A0016236biological_processmacroautophagy
A0016887molecular_functionATP hydrolysis activity
A0030496cellular_componentmidbody
A0031468biological_processnuclear membrane reassembly
A0032511biological_processlate endosome to vacuole transport via multivesicular body sorting pathway
A0036258biological_processmultivesicular body assembly
A0042802molecular_functionidentical protein binding
A0042803molecular_functionprotein homodimerization activity
A0045053biological_processprotein retention in Golgi apparatus
A0045324biological_processlate endosome to vacuole transport
A0061709biological_processreticulophagy
A0061764biological_processlate endosome to lysosome transport via multivesicular body sorting pathway
A0061952biological_processmidbody abscission
A0070676biological_processintralumenal vesicle formation
A0071985biological_processmultivesicular body sorting pathway
A0090148biological_processmembrane fission
A0097352biological_processautophagosome maturation
A1904949cellular_componentATPase complex
A1990621cellular_componentESCRT IV complex
B0001778biological_processplasma membrane repair
B0005515molecular_functionprotein binding
B0005524molecular_functionATP binding
B0005643cellular_componentnuclear pore
B0005737cellular_componentcytoplasm
B0005768cellular_componentendosome
B0005783cellular_componentendoplasmic reticulum
B0005886cellular_componentplasma membrane
B0006914biological_processautophagy
B0006997biological_processnucleus organization
B0007033biological_processvacuole organization
B0010008cellular_componentendosome membrane
B0015031biological_processprotein transport
B0016020cellular_componentmembrane
B0016125biological_processsterol metabolic process
B0016197biological_processendosomal transport
B0016236biological_processmacroautophagy
B0016887molecular_functionATP hydrolysis activity
B0030496cellular_componentmidbody
B0031468biological_processnuclear membrane reassembly
B0032511biological_processlate endosome to vacuole transport via multivesicular body sorting pathway
B0036258biological_processmultivesicular body assembly
B0042802molecular_functionidentical protein binding
B0042803molecular_functionprotein homodimerization activity
B0045053biological_processprotein retention in Golgi apparatus
B0045324biological_processlate endosome to vacuole transport
B0061709biological_processreticulophagy
B0061764biological_processlate endosome to lysosome transport via multivesicular body sorting pathway
B0061952biological_processmidbody abscission
B0070676biological_processintralumenal vesicle formation
B0071985biological_processmultivesicular body sorting pathway
B0090148biological_processmembrane fission
B0097352biological_processautophagosome maturation
B1904949cellular_componentATPase complex
B1990621cellular_componentESCRT IV complex
C0001778biological_processplasma membrane repair
C0005515molecular_functionprotein binding
C0005524molecular_functionATP binding
C0005643cellular_componentnuclear pore
C0005737cellular_componentcytoplasm
C0005768cellular_componentendosome
C0005783cellular_componentendoplasmic reticulum
C0005886cellular_componentplasma membrane
C0006914biological_processautophagy
C0006997biological_processnucleus organization
C0007033biological_processvacuole organization
C0010008cellular_componentendosome membrane
C0015031biological_processprotein transport
C0016020cellular_componentmembrane
C0016125biological_processsterol metabolic process
C0016197biological_processendosomal transport
C0016236biological_processmacroautophagy
C0016887molecular_functionATP hydrolysis activity
C0030496cellular_componentmidbody
C0031468biological_processnuclear membrane reassembly
C0032511biological_processlate endosome to vacuole transport via multivesicular body sorting pathway
C0036258biological_processmultivesicular body assembly
C0042802molecular_functionidentical protein binding
C0042803molecular_functionprotein homodimerization activity
C0045053biological_processprotein retention in Golgi apparatus
C0045324biological_processlate endosome to vacuole transport
C0061709biological_processreticulophagy
C0061764biological_processlate endosome to lysosome transport via multivesicular body sorting pathway
C0061952biological_processmidbody abscission
C0070676biological_processintralumenal vesicle formation
C0071985biological_processmultivesicular body sorting pathway
C0090148biological_processmembrane fission
C0097352biological_processautophagosome maturation
C1904949cellular_componentATPase complex
C1990621cellular_componentESCRT IV complex
D0001778biological_processplasma membrane repair
D0005515molecular_functionprotein binding
D0005524molecular_functionATP binding
D0005643cellular_componentnuclear pore
D0005737cellular_componentcytoplasm
D0005768cellular_componentendosome
D0005783cellular_componentendoplasmic reticulum
D0005886cellular_componentplasma membrane
D0006914biological_processautophagy
D0006997biological_processnucleus organization
D0007033biological_processvacuole organization
D0010008cellular_componentendosome membrane
D0015031biological_processprotein transport
D0016020cellular_componentmembrane
D0016125biological_processsterol metabolic process
D0016197biological_processendosomal transport
D0016236biological_processmacroautophagy
D0016887molecular_functionATP hydrolysis activity
D0030496cellular_componentmidbody
D0031468biological_processnuclear membrane reassembly
D0032511biological_processlate endosome to vacuole transport via multivesicular body sorting pathway
D0036258biological_processmultivesicular body assembly
D0042802molecular_functionidentical protein binding
D0042803molecular_functionprotein homodimerization activity
D0045053biological_processprotein retention in Golgi apparatus
D0045324biological_processlate endosome to vacuole transport
D0061709biological_processreticulophagy
D0061764biological_processlate endosome to lysosome transport via multivesicular body sorting pathway
D0061952biological_processmidbody abscission
D0070676biological_processintralumenal vesicle formation
D0071985biological_processmultivesicular body sorting pathway
D0090148biological_processmembrane fission
D0097352biological_processautophagosome maturation
D1904949cellular_componentATPase complex
D1990621cellular_componentESCRT IV complex
E0001778biological_processplasma membrane repair
E0005515molecular_functionprotein binding
E0005524molecular_functionATP binding
E0005643cellular_componentnuclear pore
E0005737cellular_componentcytoplasm
E0005768cellular_componentendosome
E0005783cellular_componentendoplasmic reticulum
E0005886cellular_componentplasma membrane
E0006914biological_processautophagy
E0006997biological_processnucleus organization
E0007033biological_processvacuole organization
E0010008cellular_componentendosome membrane
E0015031biological_processprotein transport
E0016020cellular_componentmembrane
E0016125biological_processsterol metabolic process
E0016197biological_processendosomal transport
E0016236biological_processmacroautophagy
E0016887molecular_functionATP hydrolysis activity
E0030496cellular_componentmidbody
E0031468biological_processnuclear membrane reassembly
E0032511biological_processlate endosome to vacuole transport via multivesicular body sorting pathway
E0036258biological_processmultivesicular body assembly
E0042802molecular_functionidentical protein binding
E0042803molecular_functionprotein homodimerization activity
E0045053biological_processprotein retention in Golgi apparatus
E0045324biological_processlate endosome to vacuole transport
E0061709biological_processreticulophagy
E0061764biological_processlate endosome to lysosome transport via multivesicular body sorting pathway
E0061952biological_processmidbody abscission
E0070676biological_processintralumenal vesicle formation
E0071985biological_processmultivesicular body sorting pathway
E0090148biological_processmembrane fission
E0097352biological_processautophagosome maturation
E1904949cellular_componentATPase complex
E1990621cellular_componentESCRT IV complex
F0001778biological_processplasma membrane repair
F0005515molecular_functionprotein binding
F0005524molecular_functionATP binding
F0005643cellular_componentnuclear pore
F0005737cellular_componentcytoplasm
F0005768cellular_componentendosome
F0005783cellular_componentendoplasmic reticulum
F0005886cellular_componentplasma membrane
F0006914biological_processautophagy
F0006997biological_processnucleus organization
F0007033biological_processvacuole organization
F0010008cellular_componentendosome membrane
F0015031biological_processprotein transport
F0016020cellular_componentmembrane
F0016125biological_processsterol metabolic process
F0016197biological_processendosomal transport
F0016236biological_processmacroautophagy
F0016887molecular_functionATP hydrolysis activity
F0030496cellular_componentmidbody
F0031468biological_processnuclear membrane reassembly
F0032511biological_processlate endosome to vacuole transport via multivesicular body sorting pathway
F0036258biological_processmultivesicular body assembly
F0042802molecular_functionidentical protein binding
F0042803molecular_functionprotein homodimerization activity
F0045053biological_processprotein retention in Golgi apparatus
F0045324biological_processlate endosome to vacuole transport
F0061709biological_processreticulophagy
F0061764biological_processlate endosome to lysosome transport via multivesicular body sorting pathway
F0061952biological_processmidbody abscission
F0070676biological_processintralumenal vesicle formation
F0071985biological_processmultivesicular body sorting pathway
F0090148biological_processmembrane fission
F0097352biological_processautophagosome maturation
F1904949cellular_componentATPase complex
F1990621cellular_componentESCRT IV complex
Functional Information from PDB Data
site_idAC1
Number of Residues11
Detailsbinding site for residue ATP B 501
ChainResidue
BASP134
BGLY336
BSER337
BALA136
BGLY176
BTHR177
BGLY178
BLYS179
BSER180
BTYR181
BMET307

site_idAC2
Number of Residues14
Detailsbinding site for residue ATP C 501
ChainResidue
BLEU257
BASN261
BARG289
CASP134
CPRO175
CGLY176
CTHR177
CGLY178
CLYS179
CSER180
CTYR181
CASN277
CMET307
CSER337

site_idAC3
Number of Residues15
Detailsbinding site for residue ATP D 501
ChainResidue
CASN261
CASN265
CARG289
DALA136
DGLY176
DTHR177
DGLY178
DLYS179
DSER180
DTYR181
DGLN233
DASN277
DMET307
DGLY336
DSER337

site_idAC4
Number of Residues16
Detailsbinding site for residue ATP E 501
ChainResidue
DASN261
DASN265
DARG289
EASP134
EALA136
EGLY176
ETHR177
EGLY178
ELYS179
ESER180
ETYR181
EGLN233
EASN277
EMET307
EGLY336
ESER337

site_idAC5
Number of Residues16
Detailsbinding site for residue ATP F 501
ChainResidue
EVAL263
EASN265
EARG289
FALA136
FGLY176
FTHR177
FGLY178
FLYS179
FSER180
FTYR181
FASP232
FGLN233
FASN277
FMET307
FGLY336
FSER337

Functional Information from PROSITE/UniProt
site_idPS00674
Number of Residues20
DetailsAAA AAA-protein family signature. VlVLgATNipwqLDsAIrrR
ChainResidueDetails
AVAL270-ARG289

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues6
DetailsBINDING: BINDING => ECO:0000255
ChainResidueDetails
AGLY173
BGLY173
CGLY173
DGLY173
EGLY173
FGLY173

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PDB entries from 2024-10-30

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