Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5XMI

Cryo-EM Structure of the ATP-bound VPS4 mutant-E233Q hexamer (masked)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0001778	biological_process	plasma membrane repair
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005768	cellular_component	endosome
A	0005783	cellular_component	endoplasmic reticulum
A	0005886	cellular_component	plasma membrane
A	0006914	biological_process	autophagy
A	0006997	biological_process	nucleus organization
A	0007033	biological_process	vacuole organization
A	0010008	cellular_component	endosome membrane
A	0015031	biological_process	protein transport
A	0016020	cellular_component	membrane
A	0016125	biological_process	sterol metabolic process
A	0016197	biological_process	endosomal transport
A	0016236	biological_process	macroautophagy
A	0016887	molecular_function	ATP hydrolysis activity
A	0030496	cellular_component	midbody
A	0031468	biological_process	nuclear membrane reassembly
A	0032511	biological_process	late endosome to vacuole transport via multivesicular body sorting pathway
A	0036258	biological_process	multivesicular body assembly
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0043162	biological_process	ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
A	0045053	biological_process	protein retention in Golgi apparatus
A	0045324	biological_process	late endosome to vacuole transport
A	0061709	biological_process	reticulophagy
A	0061764	biological_process	late endosome to lysosome transport via multivesicular body sorting pathway
A	0061952	biological_process	midbody abscission
A	0070676	biological_process	intralumenal vesicle formation
A	0071985	biological_process	multivesicular body sorting pathway
A	0090148	biological_process	membrane fission
A	0090611	biological_process	ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
A	0097352	biological_process	autophagosome maturation
A	1990621	cellular_component	ESCRT IV complex
B	0000166	molecular_function	nucleotide binding
B	0001778	biological_process	plasma membrane repair
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005768	cellular_component	endosome
B	0005783	cellular_component	endoplasmic reticulum
B	0005886	cellular_component	plasma membrane
B	0006914	biological_process	autophagy
B	0006997	biological_process	nucleus organization
B	0007033	biological_process	vacuole organization
B	0010008	cellular_component	endosome membrane
B	0015031	biological_process	protein transport
B	0016020	cellular_component	membrane
B	0016125	biological_process	sterol metabolic process
B	0016197	biological_process	endosomal transport
B	0016236	biological_process	macroautophagy
B	0016887	molecular_function	ATP hydrolysis activity
B	0030496	cellular_component	midbody
B	0031468	biological_process	nuclear membrane reassembly
B	0032511	biological_process	late endosome to vacuole transport via multivesicular body sorting pathway
B	0036258	biological_process	multivesicular body assembly
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0043162	biological_process	ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
B	0045053	biological_process	protein retention in Golgi apparatus
B	0045324	biological_process	late endosome to vacuole transport
B	0061709	biological_process	reticulophagy
B	0061764	biological_process	late endosome to lysosome transport via multivesicular body sorting pathway
B	0061952	biological_process	midbody abscission
B	0070676	biological_process	intralumenal vesicle formation
B	0071985	biological_process	multivesicular body sorting pathway
B	0090148	biological_process	membrane fission
B	0090611	biological_process	ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
B	0097352	biological_process	autophagosome maturation
B	1990621	cellular_component	ESCRT IV complex
C	0000166	molecular_function	nucleotide binding
C	0001778	biological_process	plasma membrane repair
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005768	cellular_component	endosome
C	0005783	cellular_component	endoplasmic reticulum
C	0005886	cellular_component	plasma membrane
C	0006914	biological_process	autophagy
C	0006997	biological_process	nucleus organization
C	0007033	biological_process	vacuole organization
C	0010008	cellular_component	endosome membrane
C	0015031	biological_process	protein transport
C	0016020	cellular_component	membrane
C	0016125	biological_process	sterol metabolic process
C	0016197	biological_process	endosomal transport
C	0016236	biological_process	macroautophagy
C	0016887	molecular_function	ATP hydrolysis activity
C	0030496	cellular_component	midbody
C	0031468	biological_process	nuclear membrane reassembly
C	0032511	biological_process	late endosome to vacuole transport via multivesicular body sorting pathway
C	0036258	biological_process	multivesicular body assembly
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0043162	biological_process	ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
C	0045053	biological_process	protein retention in Golgi apparatus
C	0045324	biological_process	late endosome to vacuole transport
C	0061709	biological_process	reticulophagy
C	0061764	biological_process	late endosome to lysosome transport via multivesicular body sorting pathway
C	0061952	biological_process	midbody abscission
C	0070676	biological_process	intralumenal vesicle formation
C	0071985	biological_process	multivesicular body sorting pathway
C	0090148	biological_process	membrane fission
C	0090611	biological_process	ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
C	0097352	biological_process	autophagosome maturation
C	1990621	cellular_component	ESCRT IV complex
D	0000166	molecular_function	nucleotide binding
D	0001778	biological_process	plasma membrane repair
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005768	cellular_component	endosome
D	0005783	cellular_component	endoplasmic reticulum
D	0005886	cellular_component	plasma membrane
D	0006914	biological_process	autophagy
D	0006997	biological_process	nucleus organization
D	0007033	biological_process	vacuole organization
D	0010008	cellular_component	endosome membrane
D	0015031	biological_process	protein transport
D	0016020	cellular_component	membrane
D	0016125	biological_process	sterol metabolic process
D	0016197	biological_process	endosomal transport
D	0016236	biological_process	macroautophagy
D	0016887	molecular_function	ATP hydrolysis activity
D	0030496	cellular_component	midbody
D	0031468	biological_process	nuclear membrane reassembly
D	0032511	biological_process	late endosome to vacuole transport via multivesicular body sorting pathway
D	0036258	biological_process	multivesicular body assembly
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0043162	biological_process	ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
D	0045053	biological_process	protein retention in Golgi apparatus
D	0045324	biological_process	late endosome to vacuole transport
D	0061709	biological_process	reticulophagy
D	0061764	biological_process	late endosome to lysosome transport via multivesicular body sorting pathway
D	0061952	biological_process	midbody abscission
D	0070676	biological_process	intralumenal vesicle formation
D	0071985	biological_process	multivesicular body sorting pathway
D	0090148	biological_process	membrane fission
D	0090611	biological_process	ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
D	0097352	biological_process	autophagosome maturation
D	1990621	cellular_component	ESCRT IV complex
E	0000166	molecular_function	nucleotide binding
E	0001778	biological_process	plasma membrane repair
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005768	cellular_component	endosome
E	0005783	cellular_component	endoplasmic reticulum
E	0005886	cellular_component	plasma membrane
E	0006914	biological_process	autophagy
E	0006997	biological_process	nucleus organization
E	0007033	biological_process	vacuole organization
E	0010008	cellular_component	endosome membrane
E	0015031	biological_process	protein transport
E	0016020	cellular_component	membrane
E	0016125	biological_process	sterol metabolic process
E	0016197	biological_process	endosomal transport
E	0016236	biological_process	macroautophagy
E	0016887	molecular_function	ATP hydrolysis activity
E	0030496	cellular_component	midbody
E	0031468	biological_process	nuclear membrane reassembly
E	0032511	biological_process	late endosome to vacuole transport via multivesicular body sorting pathway
E	0036258	biological_process	multivesicular body assembly
E	0042802	molecular_function	identical protein binding
E	0042803	molecular_function	protein homodimerization activity
E	0043162	biological_process	ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
E	0045053	biological_process	protein retention in Golgi apparatus
E	0045324	biological_process	late endosome to vacuole transport
E	0061709	biological_process	reticulophagy
E	0061764	biological_process	late endosome to lysosome transport via multivesicular body sorting pathway
E	0061952	biological_process	midbody abscission
E	0070676	biological_process	intralumenal vesicle formation
E	0071985	biological_process	multivesicular body sorting pathway
E	0090148	biological_process	membrane fission
E	0090611	biological_process	ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
E	0097352	biological_process	autophagosome maturation
E	1990621	cellular_component	ESCRT IV complex
F	0000166	molecular_function	nucleotide binding
F	0001778	biological_process	plasma membrane repair
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005768	cellular_component	endosome
F	0005783	cellular_component	endoplasmic reticulum
F	0005886	cellular_component	plasma membrane
F	0006914	biological_process	autophagy
F	0006997	biological_process	nucleus organization
F	0007033	biological_process	vacuole organization
F	0010008	cellular_component	endosome membrane
F	0015031	biological_process	protein transport
F	0016020	cellular_component	membrane
F	0016125	biological_process	sterol metabolic process
F	0016197	biological_process	endosomal transport
F	0016236	biological_process	macroautophagy
F	0016887	molecular_function	ATP hydrolysis activity
F	0030496	cellular_component	midbody
F	0031468	biological_process	nuclear membrane reassembly
F	0032511	biological_process	late endosome to vacuole transport via multivesicular body sorting pathway
F	0036258	biological_process	multivesicular body assembly
F	0042802	molecular_function	identical protein binding
F	0042803	molecular_function	protein homodimerization activity
F	0043162	biological_process	ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
F	0045053	biological_process	protein retention in Golgi apparatus
F	0045324	biological_process	late endosome to vacuole transport
F	0061709	biological_process	reticulophagy
F	0061764	biological_process	late endosome to lysosome transport via multivesicular body sorting pathway
F	0061952	biological_process	midbody abscission
F	0070676	biological_process	intralumenal vesicle formation
F	0071985	biological_process	multivesicular body sorting pathway
F	0090148	biological_process	membrane fission
F	0090611	biological_process	ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
F	0097352	biological_process	autophagosome maturation
F	1990621	cellular_component	ESCRT IV complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	binding site for residue ATP B 501

Chain	Residue
B	ASP134
B	GLY336
B	SER337
B	ALA136
B	GLY176
B	THR177
B	GLY178
B	LYS179
B	SER180
B	TYR181
B	MET307

site_id	AC2
Number of Residues	14
Details	binding site for residue ATP C 501

Chain	Residue
B	LEU257
B	ASN261
B	ARG289
C	ASP134
C	PRO175
C	GLY176
C	THR177
C	GLY178
C	LYS179
C	SER180
C	TYR181
C	ASN277
C	MET307
C	SER337

site_id	AC3
Number of Residues	15
Details	binding site for residue ATP D 501

Chain	Residue
C	ASN261
C	ASN265
C	ARG289
D	ALA136
D	GLY176
D	THR177
D	GLY178
D	LYS179
D	SER180
D	TYR181
D	GLN233
D	ASN277
D	MET307
D	GLY336
D	SER337

site_id	AC4
Number of Residues	16
Details	binding site for residue ATP E 501

Chain	Residue
D	ASN261
D	ASN265
D	ARG289
E	ASP134
E	ALA136
E	GLY176
E	THR177
E	GLY178
E	LYS179
E	SER180
E	TYR181
E	GLN233
E	ASN277
E	MET307
E	GLY336
E	SER337

site_id	AC5
Number of Residues	16
Details	binding site for residue ATP F 501

Chain	Residue
E	VAL263
E	ASN265
E	ARG289
F	ALA136
F	GLY176
F	THR177
F	GLY178
F	LYS179
F	SER180
F	TYR181
F	ASP232
F	GLN233
F	ASN277
F	MET307
F	GLY336
F	SER337

Functional Information from PROSITE/UniProt

site_id	PS00674
Number of Residues	20
Details	AAA AAA-protein family signature. VlVLgATNipwqLDsAIrrR

Chain	Residue	Details
A	VAL270-ARG289

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	234
Details	Region: {"description":"Disordered","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	96
Details	Compositional bias: {"description":"Basic and acidic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	42
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)