5X69

Human thymidylate synthase with a fragment bound in the dimer interface

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
A	0004799	molecular_function	thymidylate synthase activity
A	0005515	molecular_function	protein binding
A	0005542	molecular_function	folic acid binding
A	0005634	cellular_component	nucleus
A	0005657	cellular_component	replication fork
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005743	cellular_component	mitochondrial inner membrane
A	0005759	cellular_component	mitochondrial matrix
A	0005829	cellular_component	cytosol
A	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
A	0006231	biological_process	dTMP biosynthetic process
A	0006235	biological_process	dTTP biosynthetic process
A	0006260	biological_process	DNA replication
A	0008168	molecular_function	methyltransferase activity
A	0009165	biological_process	nucleotide biosynthetic process
A	0016740	molecular_function	transferase activity
A	0016741	molecular_function	transferase activity, transferring one-carbon groups
A	0017148	biological_process	negative regulation of translation
A	0032259	biological_process	methylation
A	0035999	biological_process	tetrahydrofolate interconversion
A	0046653	biological_process	tetrahydrofolate metabolic process
A	0071897	biological_process	DNA biosynthetic process
A	1990825	molecular_function	sequence-specific mRNA binding
B	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
B	0004799	molecular_function	thymidylate synthase activity
B	0005515	molecular_function	protein binding
B	0005542	molecular_function	folic acid binding
B	0005634	cellular_component	nucleus
B	0005657	cellular_component	replication fork
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005743	cellular_component	mitochondrial inner membrane
B	0005759	cellular_component	mitochondrial matrix
B	0005829	cellular_component	cytosol
B	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
B	0006231	biological_process	dTMP biosynthetic process
B	0006235	biological_process	dTTP biosynthetic process
B	0006260	biological_process	DNA replication
B	0008168	molecular_function	methyltransferase activity
B	0009165	biological_process	nucleotide biosynthetic process
B	0016740	molecular_function	transferase activity
B	0016741	molecular_function	transferase activity, transferring one-carbon groups
B	0017148	biological_process	negative regulation of translation
B	0032259	biological_process	methylation
B	0035999	biological_process	tetrahydrofolate interconversion
B	0046653	biological_process	tetrahydrofolate metabolic process
B	0071897	biological_process	DNA biosynthetic process
B	1990825	molecular_function	sequence-specific mRNA binding
C	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
C	0004799	molecular_function	thymidylate synthase activity
C	0005515	molecular_function	protein binding
C	0005542	molecular_function	folic acid binding
C	0005634	cellular_component	nucleus
C	0005657	cellular_component	replication fork
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005743	cellular_component	mitochondrial inner membrane
C	0005759	cellular_component	mitochondrial matrix
C	0005829	cellular_component	cytosol
C	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
C	0006231	biological_process	dTMP biosynthetic process
C	0006235	biological_process	dTTP biosynthetic process
C	0006260	biological_process	DNA replication
C	0008168	molecular_function	methyltransferase activity
C	0009165	biological_process	nucleotide biosynthetic process
C	0016740	molecular_function	transferase activity
C	0016741	molecular_function	transferase activity, transferring one-carbon groups
C	0017148	biological_process	negative regulation of translation
C	0032259	biological_process	methylation
C	0035999	biological_process	tetrahydrofolate interconversion
C	0046653	biological_process	tetrahydrofolate metabolic process
C	0071897	biological_process	DNA biosynthetic process
C	1990825	molecular_function	sequence-specific mRNA binding
D	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
D	0004799	molecular_function	thymidylate synthase activity
D	0005515	molecular_function	protein binding
D	0005542	molecular_function	folic acid binding
D	0005634	cellular_component	nucleus
D	0005657	cellular_component	replication fork
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005743	cellular_component	mitochondrial inner membrane
D	0005759	cellular_component	mitochondrial matrix
D	0005829	cellular_component	cytosol
D	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
D	0006231	biological_process	dTMP biosynthetic process
D	0006235	biological_process	dTTP biosynthetic process
D	0006260	biological_process	DNA replication
D	0008168	molecular_function	methyltransferase activity
D	0009165	biological_process	nucleotide biosynthetic process
D	0016740	molecular_function	transferase activity
D	0016741	molecular_function	transferase activity, transferring one-carbon groups
D	0017148	biological_process	negative regulation of translation
D	0032259	biological_process	methylation
D	0035999	biological_process	tetrahydrofolate interconversion
D	0046653	biological_process	tetrahydrofolate metabolic process
D	0071897	biological_process	DNA biosynthetic process
D	1990825	molecular_function	sequence-specific mRNA binding
E	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
E	0004799	molecular_function	thymidylate synthase activity
E	0005515	molecular_function	protein binding
E	0005542	molecular_function	folic acid binding
E	0005634	cellular_component	nucleus
E	0005657	cellular_component	replication fork
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005743	cellular_component	mitochondrial inner membrane
E	0005759	cellular_component	mitochondrial matrix
E	0005829	cellular_component	cytosol
E	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
E	0006231	biological_process	dTMP biosynthetic process
E	0006235	biological_process	dTTP biosynthetic process
E	0006260	biological_process	DNA replication
E	0008168	molecular_function	methyltransferase activity
E	0009165	biological_process	nucleotide biosynthetic process
E	0016740	molecular_function	transferase activity
E	0016741	molecular_function	transferase activity, transferring one-carbon groups
E	0017148	biological_process	negative regulation of translation
E	0032259	biological_process	methylation
E	0035999	biological_process	tetrahydrofolate interconversion
E	0046653	biological_process	tetrahydrofolate metabolic process
E	0071897	biological_process	DNA biosynthetic process
E	1990825	molecular_function	sequence-specific mRNA binding
F	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
F	0004799	molecular_function	thymidylate synthase activity
F	0005515	molecular_function	protein binding
F	0005542	molecular_function	folic acid binding
F	0005634	cellular_component	nucleus
F	0005657	cellular_component	replication fork
F	0005737	cellular_component	cytoplasm
F	0005739	cellular_component	mitochondrion
F	0005743	cellular_component	mitochondrial inner membrane
F	0005759	cellular_component	mitochondrial matrix
F	0005829	cellular_component	cytosol
F	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
F	0006231	biological_process	dTMP biosynthetic process
F	0006235	biological_process	dTTP biosynthetic process
F	0006260	biological_process	DNA replication
F	0008168	molecular_function	methyltransferase activity
F	0009165	biological_process	nucleotide biosynthetic process
F	0016740	molecular_function	transferase activity
F	0016741	molecular_function	transferase activity, transferring one-carbon groups
F	0017148	biological_process	negative regulation of translation
F	0032259	biological_process	methylation
F	0035999	biological_process	tetrahydrofolate interconversion
F	0046653	biological_process	tetrahydrofolate metabolic process
F	0071897	biological_process	DNA biosynthetic process
F	1990825	molecular_function	sequence-specific mRNA binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	binding site for residue 7ZC A 401

Chain	Residue
A	GLY143
A	ASN183
A	ARG185
D	GLY143
D	ALA144
D	ASN183
D	ARG185
D	ASP186

---

site_id	AC2
Number of Residues	12
Details	binding site for residue UMP A 402

Chain	Residue
A	HIS196
A	GLN214
A	ARG215
A	SER216
A	ASP218
A	ASN226
A	HIS256
A	TYR258
A	HOH502
D	ARG175
D	ARG176
A	CYS195

---

site_id	AC3
Number of Residues	12
Details	binding site for residue UMP B 401

Chain	Residue
B	ARG50
B	CYS195
B	HIS196
B	GLN214
B	ARG215
B	SER216
B	ASP218
B	ASN226
B	HIS256
B	TYR258
E	ARG175
E	ARG176

---

site_id	AC4
Number of Residues	12
Details	binding site for residue UMP D 401

Chain	Residue
A	ARG175
A	ARG176
D	ARG50
D	CYS195
D	HIS196
D	GLN214
D	ARG215
D	SER216
D	ASP218
D	ASN226
D	HIS256
D	TYR258

---

site_id	AC5
Number of Residues	8
Details	binding site for residue 7ZC E 401

Chain	Residue
B	GLY143
B	GLU145
B	ASN183
B	ARG185
E	GLY143
E	ASN183
E	ARG185
E	ASP186

---

site_id	AC6
Number of Residues	13
Details	binding site for residue UMP F 401

Chain	Residue
C	ARG175
C	ARG176
F	ARG50
F	CYS195
F	GLN214
F	ARG215
F	SER216
F	GLY217
F	ASP218
F	ASN226
F	HIS256
F	TYR258
F	HOH502

---

Functional Information from PROSITE/UniProt

site_id	PS00091
Number of Residues	29
Details	THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImcaWNprdlplma.....LpPCHalcQFyV

Chain	Residue	Details
A	ARG175-VAL203

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	10
Details	Binding site: {"description":"in other chain","evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P45352","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	36
Details	Binding site: {"description":"in other chain","evidences":[{"source":"UniProtKB","id":"P45352","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	21
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---