5WT4

L-Cysteine-PLP intermediate of NifS from Helicobacter pylori

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0006534	biological_process	cysteine metabolic process
A	0016226	biological_process	iron-sulfur cluster assembly
A	0016740	molecular_function	transferase activity
A	0030170	molecular_function	pyridoxal phosphate binding
A	0031071	molecular_function	cysteine desulfurase activity
A	0044571	biological_process	[2Fe-2S] cluster assembly
A	0046872	molecular_function	metal ion binding
A	0051537	molecular_function	2 iron, 2 sulfur cluster binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	binding site for residue IPA A 501

Chain	Residue
A	TYR56
A	VAL67
A	ASN235

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site_id	AC2
Number of Residues	14
Details	binding site for residue C6P A 502

Chain	Residue
A	GLN183
A	SER203
A	HIS205
A	LYS206
A	GLY240
A	THR241
A	ARG354
A	HOH601
A	CYS72
A	ALA73
A	THR74
A	HIS104
A	ASN155
A	ASP180

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Cysteine persulfide intermediate => ECO:0000255|HAMAP-Rule:MF_00331

Chain	Residue	Details
A	CYS328

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site_id	SWS_FT_FI2
Number of Residues	5
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00331

Chain	Residue	Details
A	ALA73
A	ASN155
A	GLN183
A	SER203
A	THR241

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site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: via persulfide group => ECO:0000255|HAMAP-Rule:MF_00331

Chain	Residue	Details
A	CYS328

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site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000255|HAMAP-Rule:MF_00331

Chain	Residue	Details
A	LYS206

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