Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5V8F

Structural basis of MCM2-7 replicative helicase loading by ORC-Cdc6 and Cdt1

Replaces: 5UDB

Functional Information from GO Data

Chain	GOid	namespace	contents
2	0000727	biological_process	double-strand break repair via break-induced replication
2	0003677	molecular_function	DNA binding
2	0003682	molecular_function	chromatin binding
2	0003688	molecular_function	DNA replication origin binding
2	0003697	molecular_function	single-stranded DNA binding
2	0004386	molecular_function	helicase activity
2	0005515	molecular_function	protein binding
2	0005524	molecular_function	ATP binding
2	0005634	cellular_component	nucleus
2	0005654	cellular_component	nucleoplasm
2	0005656	cellular_component	nuclear pre-replicative complex
2	0005737	cellular_component	cytoplasm
2	0006260	biological_process	DNA replication
2	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
2	0006268	biological_process	DNA unwinding involved in DNA replication
2	0006270	biological_process	DNA replication initiation
2	0006271	biological_process	DNA strand elongation involved in DNA replication
2	0006279	biological_process	premeiotic DNA replication
2	0006974	biological_process	DNA damage response
2	0008270	molecular_function	zinc ion binding
2	0016787	molecular_function	hydrolase activity
2	0016887	molecular_function	ATP hydrolysis activity
2	0017116	molecular_function	single-stranded DNA helicase activity
2	0031261	cellular_component	DNA replication preinitiation complex
2	0031298	cellular_component	replication fork protection complex
2	0032508	biological_process	DNA duplex unwinding
2	0042555	cellular_component	MCM complex
2	0043138	molecular_function	3'-5' DNA helicase activity
2	0043596	cellular_component	nuclear replication fork
2	0046872	molecular_function	metal ion binding
2	0071162	cellular_component	CMG complex
2	1902975	biological_process	mitotic DNA replication initiation
2	1905775	biological_process	negative regulation of DNA helicase activity
3	0000727	biological_process	double-strand break repair via break-induced replication
3	0000781	cellular_component	chromosome, telomeric region
3	0003677	molecular_function	DNA binding
3	0003682	molecular_function	chromatin binding
3	0003688	molecular_function	DNA replication origin binding
3	0003697	molecular_function	single-stranded DNA binding
3	0004386	molecular_function	helicase activity
3	0005515	molecular_function	protein binding
3	0005524	molecular_function	ATP binding
3	0005634	cellular_component	nucleus
3	0005654	cellular_component	nucleoplasm
3	0005656	cellular_component	nuclear pre-replicative complex
3	0005737	cellular_component	cytoplasm
3	0006260	biological_process	DNA replication
3	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
3	0006268	biological_process	DNA unwinding involved in DNA replication
3	0006270	biological_process	DNA replication initiation
3	0006271	biological_process	DNA strand elongation involved in DNA replication
3	0006279	biological_process	premeiotic DNA replication
3	0016787	molecular_function	hydrolase activity
3	0016887	molecular_function	ATP hydrolysis activity
3	0017116	molecular_function	single-stranded DNA helicase activity
3	0030466	biological_process	silent mating-type cassette heterochromatin formation
3	0031261	cellular_component	DNA replication preinitiation complex
3	0031298	cellular_component	replication fork protection complex
3	0031509	biological_process	subtelomeric heterochromatin formation
3	0032508	biological_process	DNA duplex unwinding
3	0042555	cellular_component	MCM complex
3	0043596	cellular_component	nuclear replication fork
3	0071162	cellular_component	CMG complex
3	1902975	biological_process	mitotic DNA replication initiation
3	1904931	molecular_function	MCM complex binding
4	0000727	biological_process	double-strand break repair via break-induced replication
4	0003677	molecular_function	DNA binding
4	0003678	molecular_function	DNA helicase activity
4	0003688	molecular_function	DNA replication origin binding
4	0003697	molecular_function	single-stranded DNA binding
4	0004386	molecular_function	helicase activity
4	0005515	molecular_function	protein binding
4	0005524	molecular_function	ATP binding
4	0005634	cellular_component	nucleus
4	0005654	cellular_component	nucleoplasm
4	0005656	cellular_component	nuclear pre-replicative complex
4	0005737	cellular_component	cytoplasm
4	0006260	biological_process	DNA replication
4	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
4	0006268	biological_process	DNA unwinding involved in DNA replication
4	0006270	biological_process	DNA replication initiation
4	0006271	biological_process	DNA strand elongation involved in DNA replication
4	0006279	biological_process	premeiotic DNA replication
4	0008270	molecular_function	zinc ion binding
4	0009378	molecular_function	four-way junction helicase activity
4	0016787	molecular_function	hydrolase activity
4	0016887	molecular_function	ATP hydrolysis activity
4	0017116	molecular_function	single-stranded DNA helicase activity
4	0031261	cellular_component	DNA replication preinitiation complex
4	0031298	cellular_component	replication fork protection complex
4	0032508	biological_process	DNA duplex unwinding
4	0033260	biological_process	nuclear DNA replication
4	0042555	cellular_component	MCM complex
4	0043138	molecular_function	3'-5' DNA helicase activity
4	0043596	cellular_component	nuclear replication fork
4	0071162	cellular_component	CMG complex
4	0097373	cellular_component	MCM core complex
4	1902975	biological_process	mitotic DNA replication initiation
5	0000727	biological_process	double-strand break repair via break-induced replication
5	0000781	cellular_component	chromosome, telomeric region
5	0003677	molecular_function	DNA binding
5	0003682	molecular_function	chromatin binding
5	0003688	molecular_function	DNA replication origin binding
5	0003697	molecular_function	single-stranded DNA binding
5	0004386	molecular_function	helicase activity
5	0005515	molecular_function	protein binding
5	0005524	molecular_function	ATP binding
5	0005634	cellular_component	nucleus
5	0005654	cellular_component	nucleoplasm
5	0005656	cellular_component	nuclear pre-replicative complex
5	0005737	cellular_component	cytoplasm
5	0006260	biological_process	DNA replication
5	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
5	0006268	biological_process	DNA unwinding involved in DNA replication
5	0006270	biological_process	DNA replication initiation
5	0006279	biological_process	premeiotic DNA replication
5	0006368	biological_process	transcription elongation by RNA polymerase II
5	0008270	molecular_function	zinc ion binding
5	0016787	molecular_function	hydrolase activity
5	0016887	molecular_function	ATP hydrolysis activity
5	0017116	molecular_function	single-stranded DNA helicase activity
5	0030174	biological_process	regulation of DNA-templated DNA replication initiation
5	0031261	cellular_component	DNA replication preinitiation complex
5	0031298	cellular_component	replication fork protection complex
5	0031507	biological_process	heterochromatin formation
5	0031509	biological_process	subtelomeric heterochromatin formation
5	0032508	biological_process	DNA duplex unwinding
5	0033260	biological_process	nuclear DNA replication
5	0042555	cellular_component	MCM complex
5	0043138	molecular_function	3'-5' DNA helicase activity
5	0043596	cellular_component	nuclear replication fork
5	0071162	cellular_component	CMG complex
6	0000727	biological_process	double-strand break repair via break-induced replication
6	0003677	molecular_function	DNA binding
6	0003678	molecular_function	DNA helicase activity
6	0003688	molecular_function	DNA replication origin binding
6	0003697	molecular_function	single-stranded DNA binding
6	0004386	molecular_function	helicase activity
6	0005515	molecular_function	protein binding
6	0005524	molecular_function	ATP binding
6	0005634	cellular_component	nucleus
6	0005654	cellular_component	nucleoplasm
6	0005656	cellular_component	nuclear pre-replicative complex
6	0005737	cellular_component	cytoplasm
6	0006260	biological_process	DNA replication
6	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
6	0006268	biological_process	DNA unwinding involved in DNA replication
6	0006270	biological_process	DNA replication initiation
6	0006271	biological_process	DNA strand elongation involved in DNA replication
6	0006279	biological_process	premeiotic DNA replication
6	0008270	molecular_function	zinc ion binding
6	0009378	molecular_function	four-way junction helicase activity
6	0016787	molecular_function	hydrolase activity
6	0016887	molecular_function	ATP hydrolysis activity
6	0017116	molecular_function	single-stranded DNA helicase activity
6	0031261	cellular_component	DNA replication preinitiation complex
6	0031298	cellular_component	replication fork protection complex
6	0032508	biological_process	DNA duplex unwinding
6	0042555	cellular_component	MCM complex
6	0043138	molecular_function	3'-5' DNA helicase activity
6	0043596	cellular_component	nuclear replication fork
6	0071162	cellular_component	CMG complex
6	0097373	cellular_component	MCM core complex
6	1902969	biological_process	mitotic DNA replication
6	1990518	molecular_function	single-stranded 3'-5' DNA helicase activity
7	0000727	biological_process	double-strand break repair via break-induced replication
7	0000781	cellular_component	chromosome, telomeric region
7	0003677	molecular_function	DNA binding
7	0003678	molecular_function	DNA helicase activity
7	0003682	molecular_function	chromatin binding
7	0003688	molecular_function	DNA replication origin binding
7	0003697	molecular_function	single-stranded DNA binding
7	0004386	molecular_function	helicase activity
7	0005515	molecular_function	protein binding
7	0005524	molecular_function	ATP binding
7	0005634	cellular_component	nucleus
7	0005654	cellular_component	nucleoplasm
7	0005656	cellular_component	nuclear pre-replicative complex
7	0005737	cellular_component	cytoplasm
7	0006260	biological_process	DNA replication
7	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
7	0006268	biological_process	DNA unwinding involved in DNA replication
7	0006270	biological_process	DNA replication initiation
7	0006271	biological_process	DNA strand elongation involved in DNA replication
7	0006279	biological_process	premeiotic DNA replication
7	0008270	molecular_function	zinc ion binding
7	0009378	molecular_function	four-way junction helicase activity
7	0016787	molecular_function	hydrolase activity
7	0016887	molecular_function	ATP hydrolysis activity
7	0017116	molecular_function	single-stranded DNA helicase activity
7	0030466	biological_process	silent mating-type cassette heterochromatin formation
7	0031261	cellular_component	DNA replication preinitiation complex
7	0031298	cellular_component	replication fork protection complex
7	0031509	biological_process	subtelomeric heterochromatin formation
7	0032508	biological_process	DNA duplex unwinding
7	0033260	biological_process	nuclear DNA replication
7	0042555	cellular_component	MCM complex
7	0043138	molecular_function	3'-5' DNA helicase activity
7	0043596	cellular_component	nuclear replication fork
7	0071162	cellular_component	CMG complex
7	0097373	cellular_component	MCM core complex
7	1904931	molecular_function	MCM complex binding
8	0000727	biological_process	double-strand break repair via break-induced replication
8	0003677	molecular_function	DNA binding
8	0003688	molecular_function	DNA replication origin binding
8	0005515	molecular_function	protein binding
8	0005634	cellular_component	nucleus
8	0005654	cellular_component	nucleoplasm
8	0005656	cellular_component	nuclear pre-replicative complex
8	0005737	cellular_component	cytoplasm
8	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
8	0030174	biological_process	regulation of DNA-templated DNA replication initiation
8	0051301	biological_process	cell division
9	0000082	biological_process	G1/S transition of mitotic cell cycle
9	0003682	molecular_function	chromatin binding
9	0003688	molecular_function	DNA replication origin binding
9	0003924	molecular_function	GTPase activity
9	0004861	molecular_function	cyclin-dependent protein serine/threonine kinase inhibitor activity
9	0005515	molecular_function	protein binding
9	0005524	molecular_function	ATP binding
9	0005525	molecular_function	GTP binding
9	0005634	cellular_component	nucleus
9	0005654	cellular_component	nucleoplasm
9	0005656	cellular_component	nuclear pre-replicative complex
9	0005694	cellular_component	chromosome
9	0005737	cellular_component	cytoplasm
9	0006260	biological_process	DNA replication
9	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
9	0006270	biological_process	DNA replication initiation
9	0016887	molecular_function	ATP hydrolysis activity
9	0030174	biological_process	regulation of DNA-templated DNA replication initiation
9	0031261	cellular_component	DNA replication preinitiation complex
9	0033314	biological_process	mitotic DNA replication checkpoint signaling
9	0051301	biological_process	cell division
9	0140530	biological_process	MCM complex loading
A	0003677	molecular_function	DNA binding
A	0003682	molecular_function	chromatin binding
A	0003688	molecular_function	DNA replication origin binding
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005656	cellular_component	nuclear pre-replicative complex
A	0005664	cellular_component	nuclear origin of replication recognition complex
A	0005694	cellular_component	chromosome
A	0006260	biological_process	DNA replication
A	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
A	0006270	biological_process	DNA replication initiation
A	0016887	molecular_function	ATP hydrolysis activity
A	0030466	biological_process	silent mating-type cassette heterochromatin formation
A	0031261	cellular_component	DNA replication preinitiation complex
A	0031491	molecular_function	nucleosome binding
A	0032993	cellular_component	protein-DNA complex
A	0033314	biological_process	mitotic DNA replication checkpoint signaling
A	0034728	biological_process	nucleosome organization
A	0043007	biological_process	maintenance of rDNA
A	0046872	molecular_function	metal ion binding
B	0000781	cellular_component	chromosome, telomeric region
B	0000808	cellular_component	origin recognition complex
B	0003682	molecular_function	chromatin binding
B	0003688	molecular_function	DNA replication origin binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005656	cellular_component	nuclear pre-replicative complex
B	0005664	cellular_component	nuclear origin of replication recognition complex
B	0006260	biological_process	DNA replication
B	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
B	0006270	biological_process	DNA replication initiation
B	0030466	biological_process	silent mating-type cassette heterochromatin formation
B	0031261	cellular_component	DNA replication preinitiation complex
B	0031509	biological_process	subtelomeric heterochromatin formation
C	0003677	molecular_function	DNA binding
C	0003682	molecular_function	chromatin binding
C	0003688	molecular_function	DNA replication origin binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005656	cellular_component	nuclear pre-replicative complex
C	0005664	cellular_component	nuclear origin of replication recognition complex
C	0006260	biological_process	DNA replication
C	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
C	0006270	biological_process	DNA replication initiation
C	0030466	biological_process	silent mating-type cassette heterochromatin formation
C	0031261	cellular_component	DNA replication preinitiation complex
D	0000808	cellular_component	origin recognition complex
D	0003677	molecular_function	DNA binding
D	0003688	molecular_function	DNA replication origin binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005656	cellular_component	nuclear pre-replicative complex
D	0005664	cellular_component	nuclear origin of replication recognition complex
D	0006260	biological_process	DNA replication
D	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
D	0006270	biological_process	DNA replication initiation
D	0030466	biological_process	silent mating-type cassette heterochromatin formation
D	0031261	cellular_component	DNA replication preinitiation complex
E	0000808	cellular_component	origin recognition complex
E	0003688	molecular_function	DNA replication origin binding
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005656	cellular_component	nuclear pre-replicative complex
E	0005664	cellular_component	nuclear origin of replication recognition complex
E	0006260	biological_process	DNA replication
E	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
E	0006270	biological_process	DNA replication initiation
E	0006275	biological_process	regulation of DNA replication
E	0030466	biological_process	silent mating-type cassette heterochromatin formation
E	0031261	cellular_component	DNA replication preinitiation complex
E	0035361	cellular_component	Cul8-RING ubiquitin ligase complex
F	0003677	molecular_function	DNA binding
F	0003688	molecular_function	DNA replication origin binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005656	cellular_component	nuclear pre-replicative complex
F	0005664	cellular_component	nuclear origin of replication recognition complex
F	0006260	biological_process	DNA replication
F	0006267	biological_process	pre-replicative complex assembly involved in nuclear cell cycle DNA replication
F	0006270	biological_process	DNA replication initiation
F	0030466	biological_process	silent mating-type cassette heterochromatin formation
F	0031261	cellular_component	DNA replication preinitiation complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	18
Details	binding site for residue AGS 2 2001

Chain	Residue
2	ILE505
2	GLU608
2	ASN651
2	VAL699
6	GLU657
6	GLN658
6	PRO704
6	ARG708
6	VAL797
6	ARG798
2	TYR506
2	PRO545
2	GLY546
2	THR547
2	ALA548
2	LYS549
2	SER550
2	GLN551

site_id	AC2
Number of Residues	14
Details	binding site for residue AGS 4 2001

Chain	Residue
4	ILE530
4	TYR531
4	PRO570
4	SER571
4	THR572
4	SER573
4	LYS574
4	SER575
4	GLN576
4	LEU720
7	ILE450
7	ARG593
7	PRO686
7	ARG687

site_id	AC3
Number of Residues	12
Details	binding site for residue AGS 6 1101

Chain	Residue
4	GLU650
4	ARG701
4	THR795
4	ARG796
6	SER578
6	THR579
6	SER580
6	LYS581
6	SER582
6	GLU640
6	ASN683
6	ILE731

site_id	AC4
Number of Residues	16
Details	binding site for residue AGS 7 2001

Chain	Residue
3	GLU491
3	ARG542
3	ARG700
3	GLU703
7	ILE422
7	TYR423
7	GLY460
7	ASP461
7	PRO462
7	GLY463
7	ALA465
7	LYS466
7	SER467
7	GLN468
7	ASN568
7	LEU612

site_id	AC5
Number of Residues	12
Details	binding site for residue AGS 9 2001

Chain	Residue
9	GLY108
9	PRO109
9	PRO110
9	GLY111
9	THR112
9	GLY113
9	LYS114
9	THR115
9	ALA116
9	MET296
A	SER615
A	ARG616

site_id	AC6
Number of Residues	16
Details	binding site for residue AGS A 2001

Chain	Residue
A	SER432
A	LEU449
A	PRO481
A	GLY482
A	VAL483
A	GLY484
A	LYS485
A	THR486
A	LEU487
A	GLU567
A	ILE635
A	ARG639
A	ALA703
A	ARG704
A	LEU707
D	ARG267

site_id	AC7
Number of Residues	12
Details	binding site for residue AGS E 2001

Chain	Residue
E	PHE256
E	VAL8
E	TYR38
E	SER39
E	GLY40
E	THR41
E	GLY42
E	LYS43
E	THR44
E	TYR45
E	TYR192
E	ILE200

site_id	AC8
Number of Residues	11
Details	binding site for residue AGS D 2001

Chain	Residue
D	TYR61
D	ARG104
D	GLN105
D	SER106
D	TYR107
D	LYS108
D	THR109
D	TYR110
D	GLU218
D	LYS338
E	LYS158

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DLNQSKKMNvsEF

Chain	Residue	Details
C	ASP3-PHE15

site_id	PS00847
Number of Residues	9
Details	MCM_1 MCM family signature. GICCIDEFD

Chain	Residue	Details
7	GLY519-ASP527
5	GLY475-ASP483
6	GLY634-ASP642
2	GLY602-ASP610
4	GLY627-ASP635
3	GLY468-ASP476

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:18407956

Chain	Residue	Details
F	THR146
B	THR187
A	ASP566
A	GLU567
A	ASN600
A	ARG704
7	ARG593
7	ARG687

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:19779198

Chain	Residue	Details
B	SER188
6	SER372

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:17330950, ECO:0007744\|PubMed:18407956, ECO:0007744\|PubMed:19779198

Chain	Residue	Details
A	SER237
2	SER16
2	SER23
2	SER164
2	SER170

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:17330950

Chain	Residue	Details
6	THR766

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:17330950

Chain	Residue	Details
3	THR742

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)