Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5UOE

Crystal Structure Analysis of Elbow-Engineered-Fab-Bound Human Insulin Degrading Enzyme (IDE)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001618	molecular_function	virus receptor activity
A	0004175	molecular_function	endopeptidase activity
A	0004222	molecular_function	metalloendopeptidase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005777	cellular_component	peroxisome
A	0005782	cellular_component	peroxisomal matrix
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006508	biological_process	proteolysis
A	0008237	molecular_function	metallopeptidase activity
A	0008270	molecular_function	zinc ion binding
A	0008286	biological_process	insulin receptor signaling pathway
A	0009897	cellular_component	external side of plasma membrane
A	0009986	cellular_component	cell surface
A	0010815	biological_process	bradykinin catabolic process
A	0010992	biological_process	ubiquitin recycling
A	0016323	cellular_component	basolateral plasma membrane
A	0019885	biological_process	antigen processing and presentation of endogenous peptide antigen via MHC class I
A	0030163	biological_process	protein catabolic process
A	0032092	biological_process	positive regulation of protein binding
A	0042277	molecular_function	peptide binding
A	0042447	biological_process	hormone catabolic process
A	0042803	molecular_function	protein homodimerization activity
A	0043171	biological_process	peptide catabolic process
A	0043559	molecular_function	insulin binding
A	0045732	biological_process	positive regulation of protein catabolic process
A	0046718	biological_process	symbiont entry into host cell
A	0046872	molecular_function	metal ion binding
A	0050435	biological_process	amyloid-beta metabolic process
A	0051603	biological_process	proteolysis involved in protein catabolic process
A	0070062	cellular_component	extracellular exosome
A	0097242	biological_process	amyloid-beta clearance
A	0150094	biological_process	amyloid-beta clearance by cellular catabolic process
A	1901142	biological_process	insulin metabolic process
A	1901143	biological_process	insulin catabolic process
A	1903715	biological_process	regulation of aerobic respiration
B	0001618	molecular_function	virus receptor activity
B	0004175	molecular_function	endopeptidase activity
B	0004222	molecular_function	metalloendopeptidase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005576	cellular_component	extracellular region
B	0005615	cellular_component	extracellular space
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005777	cellular_component	peroxisome
B	0005782	cellular_component	peroxisomal matrix
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006508	biological_process	proteolysis
B	0008237	molecular_function	metallopeptidase activity
B	0008270	molecular_function	zinc ion binding
B	0008286	biological_process	insulin receptor signaling pathway
B	0009897	cellular_component	external side of plasma membrane
B	0009986	cellular_component	cell surface
B	0010815	biological_process	bradykinin catabolic process
B	0010992	biological_process	ubiquitin recycling
B	0016323	cellular_component	basolateral plasma membrane
B	0019885	biological_process	antigen processing and presentation of endogenous peptide antigen via MHC class I
B	0030163	biological_process	protein catabolic process
B	0032092	biological_process	positive regulation of protein binding
B	0042277	molecular_function	peptide binding
B	0042447	biological_process	hormone catabolic process
B	0042803	molecular_function	protein homodimerization activity
B	0043171	biological_process	peptide catabolic process
B	0043559	molecular_function	insulin binding
B	0045732	biological_process	positive regulation of protein catabolic process
B	0046718	biological_process	symbiont entry into host cell
B	0046872	molecular_function	metal ion binding
B	0050435	biological_process	amyloid-beta metabolic process
B	0051603	biological_process	proteolysis involved in protein catabolic process
B	0070062	cellular_component	extracellular exosome
B	0097242	biological_process	amyloid-beta clearance
B	0150094	biological_process	amyloid-beta clearance by cellular catabolic process
B	1901142	biological_process	insulin metabolic process
B	1901143	biological_process	insulin catabolic process
B	1903715	biological_process	regulation of aerobic respiration
C	0001618	molecular_function	virus receptor activity
C	0004175	molecular_function	endopeptidase activity
C	0004222	molecular_function	metalloendopeptidase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005576	cellular_component	extracellular region
C	0005615	cellular_component	extracellular space
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005777	cellular_component	peroxisome
C	0005782	cellular_component	peroxisomal matrix
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006508	biological_process	proteolysis
C	0008237	molecular_function	metallopeptidase activity
C	0008270	molecular_function	zinc ion binding
C	0008286	biological_process	insulin receptor signaling pathway
C	0009897	cellular_component	external side of plasma membrane
C	0009986	cellular_component	cell surface
C	0010815	biological_process	bradykinin catabolic process
C	0010992	biological_process	ubiquitin recycling
C	0016323	cellular_component	basolateral plasma membrane
C	0019885	biological_process	antigen processing and presentation of endogenous peptide antigen via MHC class I
C	0030163	biological_process	protein catabolic process
C	0032092	biological_process	positive regulation of protein binding
C	0042277	molecular_function	peptide binding
C	0042447	biological_process	hormone catabolic process
C	0042803	molecular_function	protein homodimerization activity
C	0043171	biological_process	peptide catabolic process
C	0043559	molecular_function	insulin binding
C	0045732	biological_process	positive regulation of protein catabolic process
C	0046718	biological_process	symbiont entry into host cell
C	0046872	molecular_function	metal ion binding
C	0050435	biological_process	amyloid-beta metabolic process
C	0051603	biological_process	proteolysis involved in protein catabolic process
C	0070062	cellular_component	extracellular exosome
C	0097242	biological_process	amyloid-beta clearance
C	0150094	biological_process	amyloid-beta clearance by cellular catabolic process
C	1901142	biological_process	insulin metabolic process
C	1901143	biological_process	insulin catabolic process
C	1903715	biological_process	regulation of aerobic respiration
D	0001618	molecular_function	virus receptor activity
D	0004175	molecular_function	endopeptidase activity
D	0004222	molecular_function	metalloendopeptidase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005576	cellular_component	extracellular region
D	0005615	cellular_component	extracellular space
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005777	cellular_component	peroxisome
D	0005782	cellular_component	peroxisomal matrix
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006508	biological_process	proteolysis
D	0008237	molecular_function	metallopeptidase activity
D	0008270	molecular_function	zinc ion binding
D	0008286	biological_process	insulin receptor signaling pathway
D	0009897	cellular_component	external side of plasma membrane
D	0009986	cellular_component	cell surface
D	0010815	biological_process	bradykinin catabolic process
D	0010992	biological_process	ubiquitin recycling
D	0016323	cellular_component	basolateral plasma membrane
D	0019885	biological_process	antigen processing and presentation of endogenous peptide antigen via MHC class I
D	0030163	biological_process	protein catabolic process
D	0032092	biological_process	positive regulation of protein binding
D	0042277	molecular_function	peptide binding
D	0042447	biological_process	hormone catabolic process
D	0042803	molecular_function	protein homodimerization activity
D	0043171	biological_process	peptide catabolic process
D	0043559	molecular_function	insulin binding
D	0045732	biological_process	positive regulation of protein catabolic process
D	0046718	biological_process	symbiont entry into host cell
D	0046872	molecular_function	metal ion binding
D	0050435	biological_process	amyloid-beta metabolic process
D	0051603	biological_process	proteolysis involved in protein catabolic process
D	0070062	cellular_component	extracellular exosome
D	0097242	biological_process	amyloid-beta clearance
D	0150094	biological_process	amyloid-beta clearance by cellular catabolic process
D	1901142	biological_process	insulin metabolic process
D	1901143	biological_process	insulin catabolic process
D	1903715	biological_process	regulation of aerobic respiration
E	0001618	molecular_function	virus receptor activity
E	0004175	molecular_function	endopeptidase activity
E	0004222	molecular_function	metalloendopeptidase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005576	cellular_component	extracellular region
E	0005615	cellular_component	extracellular space
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005777	cellular_component	peroxisome
E	0005782	cellular_component	peroxisomal matrix
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006508	biological_process	proteolysis
E	0008237	molecular_function	metallopeptidase activity
E	0008270	molecular_function	zinc ion binding
E	0008286	biological_process	insulin receptor signaling pathway
E	0009897	cellular_component	external side of plasma membrane
E	0009986	cellular_component	cell surface
E	0010815	biological_process	bradykinin catabolic process
E	0010992	biological_process	ubiquitin recycling
E	0016323	cellular_component	basolateral plasma membrane
E	0019885	biological_process	antigen processing and presentation of endogenous peptide antigen via MHC class I
E	0030163	biological_process	protein catabolic process
E	0032092	biological_process	positive regulation of protein binding
E	0042277	molecular_function	peptide binding
E	0042447	biological_process	hormone catabolic process
E	0042803	molecular_function	protein homodimerization activity
E	0043171	biological_process	peptide catabolic process
E	0043559	molecular_function	insulin binding
E	0045732	biological_process	positive regulation of protein catabolic process
E	0046718	biological_process	symbiont entry into host cell
E	0046872	molecular_function	metal ion binding
E	0050435	biological_process	amyloid-beta metabolic process
E	0051603	biological_process	proteolysis involved in protein catabolic process
E	0070062	cellular_component	extracellular exosome
E	0097242	biological_process	amyloid-beta clearance
E	0150094	biological_process	amyloid-beta clearance by cellular catabolic process
E	1901142	biological_process	insulin metabolic process
E	1901143	biological_process	insulin catabolic process
E	1903715	biological_process	regulation of aerobic respiration

Functional Information from PROSITE/UniProt

site_id	PS00143
Number of Residues	24
Details	INSULINASE Insulinase family, zinc-binding region signature. GslsdppniaGlSHFLEHMlFlGT

Chain	Residue	Details
A	GLY95-THR118

site_id	PS00290
Number of Residues	7
Details	IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. YICNVNH

Chain	Residue	Details
H	TYR202-HIS208
L	TYR193-HIS199

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	5
Details	ACT_SITE: Proton acceptor => ECO:0000255\|PROSITE-ProRule:PRU10096, ECO:0000305\|PubMed:10684867, ECO:0000305\|PubMed:17051221, ECO:0000305\|PubMed:19321446, ECO:0000305\|PubMed:23922390

Chain	Residue	Details
A	GLU111
B	GLU111
C	GLU111
D	GLU111
E	GLU111

site_id	SWS_FT_FI2
Number of Residues	15
Details	BINDING: BINDING => ECO:0000255\|PROSITE-ProRule:PRU10096, ECO:0000269\|PubMed:17051221, ECO:0000269\|PubMed:17613531, ECO:0000269\|PubMed:18986166, ECO:0000269\|PubMed:19321446, ECO:0007744\|PDB:2G54, ECO:0007744\|PDB:2JG4, ECO:0007744\|PDB:3CWW, ECO:0007744\|PDB:3N56, ECO:0007744\|PDB:3N57

Chain	Residue	Details
A	HIS108
D	HIS108
D	HIS112
D	GLU189
E	HIS108
E	HIS112
E	GLU189
A	HIS112
A	GLU189
B	HIS108
B	HIS112
B	GLU189
C	HIS108
C	HIS112
C	GLU189

site_id	SWS_FT_FI3
Number of Residues	5
Details	BINDING: in the exosite

Chain	Residue	Details
A	HIS336
B	HIS336
C	HIS336
D	HIS336
E	HIS336

site_id	SWS_FT_FI4
Number of Residues	5
Details	BINDING: BINDING => ECO:0000269\|PubMed:18986166

Chain	Residue	Details
A	LEU359
B	LEU359
C	LEU359
D	LEU359
E	LEU359

site_id	SWS_FT_FI5
Number of Residues	10
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P35559

Chain	Residue	Details
A	ARG429
E	ASP895
A	ASP895
B	ARG429
B	ASP895
C	ARG429
C	ASP895
D	ARG429
D	ASP895
E	ARG429

site_id	SWS_FT_FI6
Number of Residues	10
Details	MOD_RES: N6-succinyllysine => ECO:0000250\|UniProtKB:Q9JHR7

Chain	Residue	Details
A	LYS192
E	LYS697
A	LYS697
B	LYS192
B	LYS697
C	LYS192
C	LYS697
D	LYS192
D	LYS697
E	LYS192

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)