5UOE

Crystal Structure Analysis of Elbow-Engineered-Fab-Bound Human Insulin Degrading Enzyme (IDE)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0001540	molecular_function	amyloid-beta binding
A	0001618	molecular_function	virus receptor activity
A	0003824	molecular_function	catalytic activity
A	0004175	molecular_function	endopeptidase activity
A	0004222	molecular_function	metalloendopeptidase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005777	cellular_component	peroxisome
A	0005782	cellular_component	peroxisomal matrix
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006508	biological_process	proteolysis
A	0008233	molecular_function	peptidase activity
A	0008237	molecular_function	metallopeptidase activity
A	0008270	molecular_function	zinc ion binding
A	0008286	biological_process	insulin receptor signaling pathway
A	0009897	cellular_component	external side of plasma membrane
A	0009986	cellular_component	cell surface
A	0010815	biological_process	bradykinin catabolic process
A	0010992	biological_process	ubiquitin recycling
A	0016323	cellular_component	basolateral plasma membrane
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0017046	molecular_function	peptide hormone binding
A	0019885	biological_process	antigen processing and presentation of endogenous peptide antigen via MHC class I
A	0030163	biological_process	protein catabolic process
A	0031597	cellular_component	cytosolic proteasome complex
A	0031626	molecular_function	beta-endorphin binding
A	0032092	biological_process	positive regulation of protein binding
A	0042277	molecular_function	peptide binding
A	0042447	biological_process	hormone catabolic process
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0043171	biological_process	peptide catabolic process
A	0043559	molecular_function	insulin binding
A	0044877	molecular_function	protein-containing complex binding
A	0045732	biological_process	positive regulation of protein catabolic process
A	0045861	biological_process	negative regulation of proteolysis
A	0046718	biological_process	symbiont entry into host cell
A	0046872	molecular_function	metal ion binding
A	0050435	biological_process	amyloid-beta metabolic process
A	0051603	biological_process	proteolysis involved in protein catabolic process
A	0070062	cellular_component	extracellular exosome
A	0097242	biological_process	amyloid-beta clearance
A	0150094	biological_process	amyloid-beta clearance by cellular catabolic process
A	1901142	biological_process	insulin metabolic process
A	1901143	biological_process	insulin catabolic process
A	1903715	biological_process	regulation of aerobic respiration
B	0000166	molecular_function	nucleotide binding
B	0001540	molecular_function	amyloid-beta binding
B	0001618	molecular_function	virus receptor activity
B	0003824	molecular_function	catalytic activity
B	0004175	molecular_function	endopeptidase activity
B	0004222	molecular_function	metalloendopeptidase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005576	cellular_component	extracellular region
B	0005615	cellular_component	extracellular space
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005777	cellular_component	peroxisome
B	0005782	cellular_component	peroxisomal matrix
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006508	biological_process	proteolysis
B	0008233	molecular_function	peptidase activity
B	0008237	molecular_function	metallopeptidase activity
B	0008270	molecular_function	zinc ion binding
B	0008286	biological_process	insulin receptor signaling pathway
B	0009897	cellular_component	external side of plasma membrane
B	0009986	cellular_component	cell surface
B	0010815	biological_process	bradykinin catabolic process
B	0010992	biological_process	ubiquitin recycling
B	0016323	cellular_component	basolateral plasma membrane
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0017046	molecular_function	peptide hormone binding
B	0019885	biological_process	antigen processing and presentation of endogenous peptide antigen via MHC class I
B	0030163	biological_process	protein catabolic process
B	0031597	cellular_component	cytosolic proteasome complex
B	0031626	molecular_function	beta-endorphin binding
B	0032092	biological_process	positive regulation of protein binding
B	0042277	molecular_function	peptide binding
B	0042447	biological_process	hormone catabolic process
B	0042802	molecular_function	identical protein binding
B	0042803	molecular_function	protein homodimerization activity
B	0043171	biological_process	peptide catabolic process
B	0043559	molecular_function	insulin binding
B	0044877	molecular_function	protein-containing complex binding
B	0045732	biological_process	positive regulation of protein catabolic process
B	0045861	biological_process	negative regulation of proteolysis
B	0046718	biological_process	symbiont entry into host cell
B	0046872	molecular_function	metal ion binding
B	0050435	biological_process	amyloid-beta metabolic process
B	0051603	biological_process	proteolysis involved in protein catabolic process
B	0070062	cellular_component	extracellular exosome
B	0097242	biological_process	amyloid-beta clearance
B	0150094	biological_process	amyloid-beta clearance by cellular catabolic process
B	1901142	biological_process	insulin metabolic process
B	1901143	biological_process	insulin catabolic process
B	1903715	biological_process	regulation of aerobic respiration
C	0000166	molecular_function	nucleotide binding
C	0001540	molecular_function	amyloid-beta binding
C	0001618	molecular_function	virus receptor activity
C	0003824	molecular_function	catalytic activity
C	0004175	molecular_function	endopeptidase activity
C	0004222	molecular_function	metalloendopeptidase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005576	cellular_component	extracellular region
C	0005615	cellular_component	extracellular space
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005777	cellular_component	peroxisome
C	0005782	cellular_component	peroxisomal matrix
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006508	biological_process	proteolysis
C	0008233	molecular_function	peptidase activity
C	0008237	molecular_function	metallopeptidase activity
C	0008270	molecular_function	zinc ion binding
C	0008286	biological_process	insulin receptor signaling pathway
C	0009897	cellular_component	external side of plasma membrane
C	0009986	cellular_component	cell surface
C	0010815	biological_process	bradykinin catabolic process
C	0010992	biological_process	ubiquitin recycling
C	0016323	cellular_component	basolateral plasma membrane
C	0016787	molecular_function	hydrolase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0017046	molecular_function	peptide hormone binding
C	0019885	biological_process	antigen processing and presentation of endogenous peptide antigen via MHC class I
C	0030163	biological_process	protein catabolic process
C	0031597	cellular_component	cytosolic proteasome complex
C	0031626	molecular_function	beta-endorphin binding
C	0032092	biological_process	positive regulation of protein binding
C	0042277	molecular_function	peptide binding
C	0042447	biological_process	hormone catabolic process
C	0042802	molecular_function	identical protein binding
C	0042803	molecular_function	protein homodimerization activity
C	0043171	biological_process	peptide catabolic process
C	0043559	molecular_function	insulin binding
C	0044877	molecular_function	protein-containing complex binding
C	0045732	biological_process	positive regulation of protein catabolic process
C	0045861	biological_process	negative regulation of proteolysis
C	0046718	biological_process	symbiont entry into host cell
C	0046872	molecular_function	metal ion binding
C	0050435	biological_process	amyloid-beta metabolic process
C	0051603	biological_process	proteolysis involved in protein catabolic process
C	0070062	cellular_component	extracellular exosome
C	0097242	biological_process	amyloid-beta clearance
C	0150094	biological_process	amyloid-beta clearance by cellular catabolic process
C	1901142	biological_process	insulin metabolic process
C	1901143	biological_process	insulin catabolic process
C	1903715	biological_process	regulation of aerobic respiration
D	0000166	molecular_function	nucleotide binding
D	0001540	molecular_function	amyloid-beta binding
D	0001618	molecular_function	virus receptor activity
D	0003824	molecular_function	catalytic activity
D	0004175	molecular_function	endopeptidase activity
D	0004222	molecular_function	metalloendopeptidase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005576	cellular_component	extracellular region
D	0005615	cellular_component	extracellular space
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005777	cellular_component	peroxisome
D	0005782	cellular_component	peroxisomal matrix
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006508	biological_process	proteolysis
D	0008233	molecular_function	peptidase activity
D	0008237	molecular_function	metallopeptidase activity
D	0008270	molecular_function	zinc ion binding
D	0008286	biological_process	insulin receptor signaling pathway
D	0009897	cellular_component	external side of plasma membrane
D	0009986	cellular_component	cell surface
D	0010815	biological_process	bradykinin catabolic process
D	0010992	biological_process	ubiquitin recycling
D	0016323	cellular_component	basolateral plasma membrane
D	0016787	molecular_function	hydrolase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0017046	molecular_function	peptide hormone binding
D	0019885	biological_process	antigen processing and presentation of endogenous peptide antigen via MHC class I
D	0030163	biological_process	protein catabolic process
D	0031597	cellular_component	cytosolic proteasome complex
D	0031626	molecular_function	beta-endorphin binding
D	0032092	biological_process	positive regulation of protein binding
D	0042277	molecular_function	peptide binding
D	0042447	biological_process	hormone catabolic process
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0043171	biological_process	peptide catabolic process
D	0043559	molecular_function	insulin binding
D	0044877	molecular_function	protein-containing complex binding
D	0045732	biological_process	positive regulation of protein catabolic process
D	0045861	biological_process	negative regulation of proteolysis
D	0046718	biological_process	symbiont entry into host cell
D	0046872	molecular_function	metal ion binding
D	0050435	biological_process	amyloid-beta metabolic process
D	0051603	biological_process	proteolysis involved in protein catabolic process
D	0070062	cellular_component	extracellular exosome
D	0097242	biological_process	amyloid-beta clearance
D	0150094	biological_process	amyloid-beta clearance by cellular catabolic process
D	1901142	biological_process	insulin metabolic process
D	1901143	biological_process	insulin catabolic process
D	1903715	biological_process	regulation of aerobic respiration
E	0000166	molecular_function	nucleotide binding
E	0001540	molecular_function	amyloid-beta binding
E	0001618	molecular_function	virus receptor activity
E	0003824	molecular_function	catalytic activity
E	0004175	molecular_function	endopeptidase activity
E	0004222	molecular_function	metalloendopeptidase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005576	cellular_component	extracellular region
E	0005615	cellular_component	extracellular space
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005777	cellular_component	peroxisome
E	0005782	cellular_component	peroxisomal matrix
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006508	biological_process	proteolysis
E	0008233	molecular_function	peptidase activity
E	0008237	molecular_function	metallopeptidase activity
E	0008270	molecular_function	zinc ion binding
E	0008286	biological_process	insulin receptor signaling pathway
E	0009897	cellular_component	external side of plasma membrane
E	0009986	cellular_component	cell surface
E	0010815	biological_process	bradykinin catabolic process
E	0010992	biological_process	ubiquitin recycling
E	0016323	cellular_component	basolateral plasma membrane
E	0016787	molecular_function	hydrolase activity
E	0016887	molecular_function	ATP hydrolysis activity
E	0017046	molecular_function	peptide hormone binding
E	0019885	biological_process	antigen processing and presentation of endogenous peptide antigen via MHC class I
E	0030163	biological_process	protein catabolic process
E	0031597	cellular_component	cytosolic proteasome complex
E	0031626	molecular_function	beta-endorphin binding
E	0032092	biological_process	positive regulation of protein binding
E	0042277	molecular_function	peptide binding
E	0042447	biological_process	hormone catabolic process
E	0042802	molecular_function	identical protein binding
E	0042803	molecular_function	protein homodimerization activity
E	0043171	biological_process	peptide catabolic process
E	0043559	molecular_function	insulin binding
E	0044877	molecular_function	protein-containing complex binding
E	0045732	biological_process	positive regulation of protein catabolic process
E	0045861	biological_process	negative regulation of proteolysis
E	0046718	biological_process	symbiont entry into host cell
E	0046872	molecular_function	metal ion binding
E	0050435	biological_process	amyloid-beta metabolic process
E	0051603	biological_process	proteolysis involved in protein catabolic process
E	0070062	cellular_component	extracellular exosome
E	0097242	biological_process	amyloid-beta clearance
E	0150094	biological_process	amyloid-beta clearance by cellular catabolic process
E	1901142	biological_process	insulin metabolic process
E	1901143	biological_process	insulin catabolic process
E	1903715	biological_process	regulation of aerobic respiration

Functional Information from PROSITE/UniProt

site_id	PS00143
Number of Residues	24
Details	INSULINASE Insulinase family, zinc-binding region signature. GslsdppniaGlSHFLEHMlFlGT

Chain	Residue	Details
A	GLY95-THR118

---

site_id	PS00290
Number of Residues	7
Details	IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. YACEVTH

Chain	Residue	Details
L	TYR193-HIS199
H	TYR202-HIS208

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	5
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU10096","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"10684867","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"17051221","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19321446","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"23922390","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	15
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU10096","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17051221","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17613531","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18986166","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19321446","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2G54","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2JG4","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3CWW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3N56","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3N57","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	30
Details	Binding site: {"description":"in the exosite"}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	20
Details	Binding site: {"evidences":[{"source":"PubMed","id":"18986166","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	35
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P35559","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	10
Details	Modified residue: {"description":"N6-succinyllysine","evidences":[{"source":"UniProtKB","id":"Q9JHR7","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	20
Details	Motif: {"description":"SlyX motif","evidences":[{"source":"UniProtKB","id":"Q9JHR7","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---