5UE9

WT DHODB with orotate bound

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004152	molecular_function	dihydroorotate dehydrogenase activity
A	0004589	molecular_function	dihydroorotate dehydrogenase (NAD+) activity
A	0005737	cellular_component	cytoplasm
A	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
A	0006221	biological_process	pyrimidine nucleotide biosynthetic process
A	0006222	biological_process	UMP biosynthetic process
A	0016491	molecular_function	oxidoreductase activity
A	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
A	0044205	biological_process	'de novo' UMP biosynthetic process
B	0006221	biological_process	pyrimidine nucleotide biosynthetic process
B	0009055	molecular_function	electron transfer activity
B	0016491	molecular_function	oxidoreductase activity
B	0044205	biological_process	'de novo' UMP biosynthetic process
B	0046872	molecular_function	metal ion binding
B	0050660	molecular_function	flavin adenine dinucleotide binding
B	0051536	molecular_function	iron-sulfur cluster binding
B	0051537	molecular_function	2 iron, 2 sulfur cluster binding
C	0004152	molecular_function	dihydroorotate dehydrogenase activity
C	0004589	molecular_function	dihydroorotate dehydrogenase (NAD+) activity
C	0005737	cellular_component	cytoplasm
C	0006207	biological_process	'de novo' pyrimidine nucleobase biosynthetic process
C	0006221	biological_process	pyrimidine nucleotide biosynthetic process
C	0006222	biological_process	UMP biosynthetic process
C	0016491	molecular_function	oxidoreductase activity
C	0016627	molecular_function	oxidoreductase activity, acting on the CH-CH group of donors
C	0044205	biological_process	'de novo' UMP biosynthetic process
D	0006221	biological_process	pyrimidine nucleotide biosynthetic process
D	0009055	molecular_function	electron transfer activity
D	0016491	molecular_function	oxidoreductase activity
D	0044205	biological_process	'de novo' UMP biosynthetic process
D	0046872	molecular_function	metal ion binding
D	0050660	molecular_function	flavin adenine dinucleotide binding
D	0051536	molecular_function	iron-sulfur cluster binding
D	0051537	molecular_function	2 iron, 2 sulfur cluster binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	binding site for residue FMN A 401

Chain	Residue
A	SER24
A	ASN197
A	THR198
A	SER221
A	GLY222
A	MET247
A	GLY248
A	GLY249
A	GLY270
A	THR271
A	ORO402
A	GLY25
A	HOH507
A	LYS48
A	ALA49
A	ASN72
A	ASN104
A	ASN132
A	LYS170
A	ILE196

---

site_id	AC2
Number of Residues	10
Details	binding site for residue ORO A 402

Chain	Residue
A	LYS48
A	ASN72
A	ALA73
A	ILE74
A	GLY75
A	LEU76
A	ASN132
A	ASN197
A	THR198
A	FMN401

---

site_id	AC3
Number of Residues	3
Details	binding site for residue CL A 403

Chain	Residue
A	THR51
A	HIS53
A	ARG55

---

site_id	AC4
Number of Residues	16
Details	binding site for residue FAD B 301

Chain	Residue
B	ARG53
B	PRO54
B	ILE55
B	SER56
B	LEU70
B	TYR71
B	ARG72
B	SER78
B	GLY79
B	THR80
B	ILE120
B	GLU221
B	SER222
B	ARG223
B	MET224
B	HOH407

---

site_id	AC5
Number of Residues	9
Details	binding site for residue FES B 302

Chain	Residue
B	ALA225
B	CYS226
B	GLY227
B	GLY229
B	CYS231
B	TYR232
B	ALA233
B	CYS234
B	CYS249

---

site_id	AC6
Number of Residues	6
Details	binding site for residue GOL B 303

Chain	Residue
B	GLY144
B	PHE145
B	ASP170
B	GLY178
B	HIS179
B	VAL180

---

site_id	AC7
Number of Residues	15
Details	binding site for residue FAD D 301

Chain	Residue
D	ARG53
D	PRO54
D	ILE55
D	SER56
D	LEU70
D	TYR71
D	ARG72
D	SER78
D	GLY79
D	THR80
D	ILE120
D	GLU221
D	SER222
D	ARG223
D	MET224

---

site_id	AC8
Number of Residues	9
Details	binding site for residue FES D 302

Chain	Residue
D	ALA225
D	CYS226
D	GLY227
D	GLY229
D	ALA230
D	CYS231
D	TYR232
D	CYS234
D	CYS249

---

site_id	AC9
Number of Residues	21
Details	binding site for residue FMN C 401

Chain	Residue
C	THR198
C	SER221
C	GLY222
C	MET247
C	GLY248
C	GLY249
C	GLY270
C	THR271
C	ORO402
C	HOH505
C	ALA23
C	SER24
C	GLY25
C	LYS48
C	ALA49
C	ASN72
C	ASN104
C	ASN132
C	LYS170
C	ILE196
C	ASN197

---

site_id	AD1
Number of Residues	8
Details	binding site for residue ORO C 402

Chain	Residue
C	ASN72
C	ILE74
C	GLY75
C	LEU76
C	ASN132
C	ASN197
C	THR198
C	FMN401

---

site_id	AD2
Number of Residues	7
Details	binding site for residue GOL C 403

Chain	Residue
C	ARG55
C	ASN78
C	PRO79
C	HOH515
D	ASN243
D	HIS244
D	ALA245

---

Functional Information from PROSITE/UniProt

site_id	PS00911
Number of Residues	20
Details	DHODEHASE_1 Dihydroorotate dehydrogenase signature 1. GsimvKATTlhpRfGNptPR

Chain	Residue	Details
A	GLY43-ARG62

---

site_id	PS00912
Number of Residues	21
Details	DHODEHASE_2 Dihydroorotate dehydrogenase signature 2. IIGmGGVeSaqdVleMYmAGA

Chain	Residue	Details
A	ILE244-ALA264

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Nucleophile"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	30
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	202
Details	Domain: {"description":"FAD-binding FR-type","evidences":[{"source":"HAMAP-Rule","id":"MF_01211","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	20
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01211","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---