5UDG
Mutant E97Q crystal structure of Bacillus subtilis QueF with a disulfide Cys 55-99
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0008616 | biological_process | queuosine biosynthetic process |
| A | 0033739 | molecular_function | preQ1 synthase activity |
| A | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0008616 | biological_process | queuosine biosynthetic process |
| B | 0033739 | molecular_function | preQ1 synthase activity |
| B | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0008616 | biological_process | queuosine biosynthetic process |
| C | 0033739 | molecular_function | preQ1 synthase activity |
| C | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0008616 | biological_process | queuosine biosynthetic process |
| D | 0033739 | molecular_function | preQ1 synthase activity |
| D | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
| E | 0005737 | cellular_component | cytoplasm |
| E | 0008616 | biological_process | queuosine biosynthetic process |
| E | 0033739 | molecular_function | preQ1 synthase activity |
| E | 0046857 | molecular_function | oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | binding site for residue MG A 201 |
| Chain | Residue |
| A | ASP162 |
| A | ARG164 |
| A | HOH340 |
| D | ARG164 |
| D | HOH311 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | binding site for residue MG A 202 |
| Chain | Residue |
| A | ASP106 |
| A | GLU109 |
| A | HOH339 |
| site_id | AC3 |
| Number of Residues | 1 |
| Details | binding site for residue PGE A 203 |
| Chain | Residue |
| A | GLY140 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | binding site for residue MG B 201 |
| Chain | Residue |
| B | ARG164 |
| B | HOH363 |
| C | ASP162 |
| C | ARG164 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | binding site for residue PGE B 202 |
| Chain | Residue |
| B | VAL38 |
| B | ASN39 |
| B | ARG40 |
| B | ASP41 |
| B | TYR143 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | binding site for residue PGE C 201 |
| Chain | Residue |
| A | GLN60 |
| A | PRO61 |
| C | LYS83 |
| C | PHE87 |
| D | GLY126 |
| site_id | AC7 |
| Number of Residues | 8 |
| Details | binding site for residue MG D 201 |
| Chain | Residue |
| A | ASP112 |
| A | HOH305 |
| A | HOH312 |
| D | ASP73 |
| D | GLU74 |
| D | HOH301 |
| D | HOH308 |
| D | HOH319 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | binding site for residue MG D 202 |
| Chain | Residue |
| A | ARG164 |
| D | ASP162 |
| D | ARG164 |
| D | HOH311 |
| site_id | AC9 |
| Number of Residues | 3 |
| Details | binding site for residue PGE D 203 |
| Chain | Residue |
| D | ASN39 |
| D | ARG114 |
| D | HOH323 |
| site_id | AD1 |
| Number of Residues | 3 |
| Details | binding site for residue MG E 201 |
| Chain | Residue |
| E | ASP162 |
| E | ARG164 |
| E | HOH328 |
| site_id | AD2 |
| Number of Residues | 5 |
| Details | binding site for residue MG E 202 |
| Chain | Residue |
| C | ASP73 |
| E | ASP73 |
| E | GLU74 |
| E | HOH308 |
| E | HOH331 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 5 |
| Details | ACT_SITE: Thioimide intermediate |
| Chain | Residue | Details |
| A | CYS55 | |
| B | CYS55 | |
| C | CYS55 | |
| D | CYS55 | |
| E | CYS55 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 5 |
| Details | ACT_SITE: Proton donor |
| Chain | Residue | Details |
| A | ASP62 | |
| B | ASP62 | |
| C | ASP62 | |
| D | ASP62 | |
| E | ASP62 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 20 |
| Details | BINDING: |
| Chain | Residue | Details |
| A | VAL77 | |
| C | HIS96 | |
| C | ASP162 | |
| C | ARG164 | |
| D | VAL77 | |
| D | HIS96 | |
| D | ASP162 | |
| D | ARG164 | |
| E | VAL77 | |
| E | HIS96 | |
| E | ASP162 | |
| A | HIS96 | |
| E | ARG164 | |
| A | ASP162 | |
| A | ARG164 | |
| B | VAL77 | |
| B | HIS96 | |
| B | ASP162 | |
| B | ARG164 | |
| C | VAL77 |
