5TT5

Escherichia coli LigA (K115M) in complex with NAD+

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003911	molecular_function	DNA ligase (NAD+) activity
A	0005515	molecular_function	protein binding
A	0005829	cellular_component	cytosol
A	0006259	biological_process	DNA metabolic process
A	0006260	biological_process	DNA replication
A	0006266	biological_process	DNA ligation
A	0006281	biological_process	DNA repair
A	0006288	biological_process	base-excision repair, DNA ligation
A	0006974	biological_process	DNA damage response
A	0016874	molecular_function	ligase activity
A	0046872	molecular_function	metal ion binding
A	0070403	molecular_function	NAD+ binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	34
Details	binding site for residue NAD A 701

Chain	Residue
A	HIS18
A	SER81
A	LEU82
A	GLU113
A	LEU114
A	MET115
A	LEU116
A	ALA120
A	ARG136
A	GLU173
A	TYR225
A	TYR22
A	VAL288
A	LYS290
A	LYS314
A	MG703
A	HOH900
A	HOH905
A	HOH949
A	HOH958
A	HOH968
A	HOH969
A	HIS23
A	HOH1031
A	HOH1041
A	HOH1083
A	HOH1182
A	HOH1275
A	PRO28
A	ILE30
A	ASP32
A	TYR35
A	ASP36
A	LEU80

---

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN A 702

Chain	Residue
A	CYS408
A	CYS411
A	CYS426
A	CYS432

---

site_id	AC3
Number of Residues	6
Details	binding site for residue MG A 703

Chain	Residue
A	NAD701
A	HOH905
A	HOH949
A	HOH958
A	HOH962
A	HOH1182

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Functional Information from PROSITE/UniProt

site_id	PS01056
Number of Residues	16
Details	DNA_LIGASE_N2 NAD-dependent DNA ligase signature 2. VGRTGaITpVarLePV

Chain	Residue	Details
A	VAL331-VAL346

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: N6-AMP-lysine intermediate => ECO:0000255|HAMAP-Rule:MF_01588, ECO:0000269|PubMed:11781321

Chain	Residue	Details
A	MET115

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site_id	SWS_FT_FI2
Number of Residues	3
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01588

Chain	Residue	Details
A	ASP32
A	GLU173
A	LYS314

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01588, ECO:0000305|PubMed:17466627

Chain	Residue	Details
A	SER81
A	GLU113
A	ARG136
A	LYS290

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01588, ECO:0000269|PubMed:17466627

Chain	Residue	Details
A	CYS408
A	CYS411
A	CYS426
A	CYS432

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	SITE: Interaction with target DNA => ECO:0000305|PubMed:17466627

Chain	Residue	Details
A	ARG487
A	SER492

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