5TJ9
2.6 Angstrom Crystal Structure of S-adenosylhomocysteinase from Cryptosporidium parvum in Complex with Aristeromycin and NAD
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004013 | molecular_function | adenosylhomocysteinase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0033353 | biological_process | S-adenosylmethionine cycle |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0004013 | molecular_function | adenosylhomocysteinase activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0033353 | biological_process | S-adenosylmethionine cycle |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0004013 | molecular_function | adenosylhomocysteinase activity |
| C | 0005829 | cellular_component | cytosol |
| C | 0006730 | biological_process | one-carbon metabolic process |
| C | 0016787 | molecular_function | hydrolase activity |
| C | 0033353 | biological_process | S-adenosylmethionine cycle |
| D | 0000166 | molecular_function | nucleotide binding |
| D | 0004013 | molecular_function | adenosylhomocysteinase activity |
| D | 0005829 | cellular_component | cytosol |
| D | 0006730 | biological_process | one-carbon metabolic process |
| D | 0016787 | molecular_function | hydrolase activity |
| D | 0033353 | biological_process | S-adenosylmethionine cycle |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 15 |
| Details | binding site for residue 7CY A 501 |
| Chain | Residue |
| A | HIS53 |
| A | HIS356 |
| A | LEU401 |
| A | THR408 |
| A | HIS410 |
| A | MET415 |
| A | NAD502 |
| A | THR55 |
| A | GLU57 |
| A | THR58 |
| A | ASP137 |
| A | GLU211 |
| A | THR212 |
| A | LYS241 |
| A | ASP245 |
| site_id | AC2 |
| Number of Residues | 31 |
| Details | binding site for residue NAD A 502 |
| Chain | Residue |
| A | THR212 |
| A | THR213 |
| A | THR214 |
| A | ASN246 |
| A | GLY275 |
| A | GLY277 |
| A | GLU278 |
| A | VAL279 |
| A | THR297 |
| A | GLU298 |
| A | ILE299 |
| A | ASP300 |
| A | CYS303 |
| A | THR331 |
| A | GLY332 |
| A | ASN333 |
| A | ILE354 |
| A | GLY355 |
| A | HIS356 |
| A | ASN403 |
| A | HIS410 |
| A | 7CY501 |
| A | HOH602 |
| A | HOH623 |
| A | HOH636 |
| A | HOH638 |
| A | HOH648 |
| A | HOH680 |
| B | GLN476 |
| B | LYS489 |
| B | TYR493 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 A 503 |
| Chain | Residue |
| A | LYS270 |
| A | ARG293 |
| A | HOH613 |
| C | GLN313 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | binding site for residue SO4 A 504 |
| Chain | Residue |
| A | ARG38 |
| A | HOH641 |
| site_id | AC5 |
| Number of Residues | 2 |
| Details | binding site for residue SO4 A 505 |
| Chain | Residue |
| A | MET3 |
| A | GLU4 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | binding site for residue P6G A 506 |
| Chain | Residue |
| A | ASN481 |
| A | VAL482 |
| A | LYS489 |
| A | SER490 |
| B | SER224 |
| site_id | AC7 |
| Number of Residues | 1 |
| Details | binding site for residue GOL A 507 |
| Chain | Residue |
| A | ILE7 |
| site_id | AC8 |
| Number of Residues | 15 |
| Details | binding site for residue 7CY B 501 |
| Chain | Residue |
| B | HIS53 |
| B | THR55 |
| B | GLU57 |
| B | THR58 |
| B | ASP137 |
| B | GLU211 |
| B | THR212 |
| B | LYS241 |
| B | ASP245 |
| B | HIS356 |
| B | THR408 |
| B | HIS410 |
| B | MET415 |
| B | PHE419 |
| B | NAD502 |
| site_id | AC9 |
| Number of Residues | 28 |
| Details | binding site for residue NAD B 502 |
| Chain | Residue |
| B | HOH628 |
| B | HOH641 |
| B | HOH651 |
| B | HOH673 |
| A | LYS489 |
| A | TYR493 |
| B | THR212 |
| B | THR213 |
| B | THR214 |
| B | ASN246 |
| B | GLY275 |
| B | GLY277 |
| B | GLU278 |
| B | VAL279 |
| B | THR297 |
| B | GLU298 |
| B | ILE299 |
| B | ASP300 |
| B | THR331 |
| B | GLY332 |
| B | ASN333 |
| B | ILE354 |
| B | GLY355 |
| B | HIS356 |
| B | ASN403 |
| B | HIS410 |
| B | 7CY501 |
| B | HOH605 |
| site_id | AD1 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 B 503 |
| Chain | Residue |
| B | LYS270 |
| B | ARG293 |
| D | GLN313 |
| site_id | AD2 |
| Number of Residues | 1 |
| Details | binding site for residue SO4 B 504 |
| Chain | Residue |
| B | TRP101 |
| site_id | AD3 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 B 505 |
| Chain | Residue |
| B | LYS2 |
| B | MET3 |
| B | GLU4 |
| B | ARG6 |
| site_id | AD4 |
| Number of Residues | 1 |
| Details | binding site for residue SO4 B 506 |
| Chain | Residue |
| B | ARG35 |
| site_id | AD5 |
| Number of Residues | 1 |
| Details | binding site for residue SO4 B 507 |
| Chain | Residue |
| B | ARG343 |
| site_id | AD6 |
| Number of Residues | 4 |
| Details | binding site for residue P6G B 508 |
| Chain | Residue |
| B | ASN481 |
| B | VAL482 |
| B | PRO487 |
| B | HOH661 |
| site_id | AD7 |
| Number of Residues | 16 |
| Details | binding site for residue 7CY C 501 |
| Chain | Residue |
| C | HIS53 |
| C | THR55 |
| C | GLU57 |
| C | THR58 |
| C | ASP137 |
| C | GLU211 |
| C | THR212 |
| C | LYS241 |
| C | ASP245 |
| C | HIS356 |
| C | THR408 |
| C | GLY409 |
| C | HIS410 |
| C | MET415 |
| C | PHE419 |
| C | NAD502 |
| site_id | AD8 |
| Number of Residues | 29 |
| Details | binding site for residue NAD C 502 |
| Chain | Residue |
| C | THR212 |
| C | THR213 |
| C | THR214 |
| C | ASN246 |
| C | GLY275 |
| C | GLY277 |
| C | GLU278 |
| C | VAL279 |
| C | THR297 |
| C | GLU298 |
| C | ILE299 |
| C | ASP300 |
| C | CYS303 |
| C | THR331 |
| C | GLY332 |
| C | ASN333 |
| C | ILE354 |
| C | GLY355 |
| C | HIS356 |
| C | ASN403 |
| C | HIS410 |
| C | 7CY501 |
| C | HOH624 |
| C | HOH628 |
| C | HOH633 |
| C | HOH653 |
| D | GLN476 |
| D | LYS489 |
| D | TYR493 |
| site_id | AD9 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 C 503 |
| Chain | Residue |
| A | GLN313 |
| C | LYS270 |
| C | ARG293 |
| C | HOH606 |
| site_id | AE1 |
| Number of Residues | 1 |
| Details | binding site for residue GOL C 504 |
| Chain | Residue |
| C | ILE7 |
| site_id | AE2 |
| Number of Residues | 15 |
| Details | binding site for residue 7CY D 501 |
| Chain | Residue |
| D | HIS53 |
| D | THR55 |
| D | GLU57 |
| D | THR58 |
| D | ASP137 |
| D | GLU211 |
| D | THR212 |
| D | LYS241 |
| D | ASP245 |
| D | HIS356 |
| D | LEU404 |
| D | THR408 |
| D | HIS410 |
| D | MET415 |
| D | NAD502 |
| site_id | AE3 |
| Number of Residues | 29 |
| Details | binding site for residue NAD D 502 |
| Chain | Residue |
| C | LYS489 |
| C | TYR493 |
| D | THR212 |
| D | THR213 |
| D | THR214 |
| D | ASN246 |
| D | GLY275 |
| D | GLY277 |
| D | GLU278 |
| D | VAL279 |
| D | THR297 |
| D | GLU298 |
| D | ILE299 |
| D | ASP300 |
| D | CYS303 |
| D | ALA330 |
| D | THR331 |
| D | GLY332 |
| D | ASN333 |
| D | ILE354 |
| D | GLY355 |
| D | HIS356 |
| D | ASN403 |
| D | HIS410 |
| D | 7CY501 |
| D | HOH641 |
| D | HOH673 |
| D | HOH682 |
| D | HOH691 |
| site_id | AE4 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 D 503 |
| Chain | Residue |
| B | GLN313 |
| D | LYS270 |
| D | ARG293 |
| D | HOH695 |
| site_id | AE5 |
| Number of Residues | 1 |
| Details | binding site for residue SO4 D 504 |
| Chain | Residue |
| D | TYR456 |
| site_id | AE6 |
| Number of Residues | 5 |
| Details | binding site for residue SO4 D 505 |
| Chain | Residue |
| D | TYR1 |
| D | LYS2 |
| D | MET3 |
| D | GLU4 |
| D | ARG6 |
| site_id | AE7 |
| Number of Residues | 1 |
| Details | binding site for residue PEG D 506 |
| Chain | Residue |
| D | ILE7 |
| site_id | AE8 |
| Number of Residues | 2 |
| Details | binding site for residue PEG D 507 |
| Chain | Residue |
| D | ASN481 |
| D | VAL482 |
| site_id | AE9 |
| Number of Residues | 1 |
| Details | binding site for residue PEG D 508 |
| Chain | Residue |
| D | ASN481 |
Functional Information from PROSITE/UniProt
| site_id | PS00738 |
| Number of Residues | 15 |
| Details | ADOHCYASE_1 S-adenosyl-L-homocysteine hydrolase signature 1. SCNiYSTQDhAAAAL |
| Chain | Residue | Details |
| A | SER76-LEU90 |
| site_id | PS00739 |
| Number of Residues | 17 |
| Details | ADOHCYASE_2 S-adenosyl-L-homocysteine hydrolase signature 2. GKkivVlGYGeVGKGc.A |
| Chain | Residue | Details |
| A | GLY268-ALA284 |
