5T0M

A histone H3K9M mutation traps histone methyltransferase Clr4 to prevent heterochromatin spreading

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002039	molecular_function	p53 binding
A	0005634	cellular_component	nucleus
A	0008270	molecular_function	zinc ion binding
A	0016279	molecular_function	protein-lysine N-methyltransferase activity
A	0042054	molecular_function	histone methyltransferase activity
B	0002039	molecular_function	p53 binding
B	0005634	cellular_component	nucleus
B	0008270	molecular_function	zinc ion binding
B	0016279	molecular_function	protein-lysine N-methyltransferase activity
B	0042054	molecular_function	histone methyltransferase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN A 1201

Chain	Residue
A	CYS974
A	CYS987
A	CYS1017
A	CYS1021

---

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN A 1202

Chain	Residue
A	CYS980
A	CYS1017
A	CYS1023
A	CYS1027

---

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN A 1203

Chain	Residue
A	CYS976
A	CYS980
A	CYS985
A	CYS974

---

site_id	AC4
Number of Residues	4
Details	binding site for residue ZN A 1204

Chain	Residue
A	CYS1115
A	CYS1168
A	CYS1170
A	CYS1175

---

site_id	AC5
Number of Residues	19
Details	binding site for residue SAM A 1205

Chain	Residue
A	MET1048
A	TRP1050
A	SER1084
A	TYR1085
A	ARG1109
A	PHE1110
A	ILE1111
A	ASN1112
A	HIS1113
A	TYR1154
A	PHE1166
A	THR1167
A	CYS1168
A	GLN1169
A	SAH1206
A	HOH1404
A	HOH1422
A	HOH1423
A	HOH1425

---

site_id	AC6
Number of Residues	19
Details	binding site for residue SAH A 1206

Chain	Residue
A	MET1048
A	TRP1050
A	SER1084
A	TYR1085
A	ARG1109
A	PHE1110
A	ILE1111
A	ASN1112
A	HIS1113
A	TYR1154
A	PHE1166
A	THR1167
A	CYS1168
A	GLN1169
A	SAM1205
A	HOH1404
A	HOH1422
A	HOH1423
A	HOH1425

---

site_id	AC7
Number of Residues	4
Details	binding site for residue ZN B 1201

Chain	Residue
B	CYS974
B	CYS987
B	CYS1017
B	CYS1021

---

site_id	AC8
Number of Residues	4
Details	binding site for residue ZN B 1202

Chain	Residue
B	CYS980
B	CYS1017
B	CYS1023
B	CYS1027

---

site_id	AC9
Number of Residues	4
Details	binding site for residue ZN B 1203

Chain	Residue
B	CYS974
B	CYS976
B	CYS980
B	CYS985

---

site_id	AD1
Number of Residues	4
Details	binding site for residue ZN B 1204

Chain	Residue
B	CYS1115
B	CYS1168
B	CYS1170
B	CYS1175

---

site_id	AD2
Number of Residues	18
Details	binding site for residue SAM B 1205

Chain	Residue
B	MET1048
B	TRP1050
B	SER1084
B	TYR1085
B	ARG1109
B	PHE1110
B	ILE1111
B	ASN1112
B	HIS1113
B	TYR1154
B	PHE1158
B	PHE1166
B	THR1167
B	CYS1168
B	GLN1169
B	HOH1354
B	HOH1402
B	HOH1413

---

site_id	AD3
Number of Residues	16
Details	binding site for Di-peptide ARG C 8 and NLE C 9

Chain	Residue
C	LYS4
C	THR6
C	ALA7
C	SER10
C	HOH105
B	TYR1067
B	ASP1074
B	ALA1077
B	ASP1078
B	LEU1086
B	PHE1087
B	ASP1088
B	PHE1152
B	TYR1154
B	ARG1157
B	PHE1158

---

site_id	AD4
Number of Residues	11
Details	binding site for Di-peptide NLE C 9 and SER C 10

Chain	Residue
B	TYR1067
B	LEU1086
B	PHE1087
B	ASP1088
B	ASN1091
B	PHE1152
B	TYR1154
C	ARG8
C	THR11
C	GLY12
C	HOH105

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	126
Details	Domain: {"description":"Pre-SET","evidences":[{"source":"PROSITE-ProRule","id":"PRU00157","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	32
Details	Domain: {"description":"Post-SET"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	25
Details	Region: {"description":"Interaction with histone H3","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	36
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Site: {"description":"Histone H3K9me binding","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	117
Details	Domain: {"description":"SET","evidences":[{"source":"PROSITE-ProRule","id":"PRU00190","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine; by HASPIN and VRK1","evidences":[{"source":"PubMed","id":"15681610","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16185088","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"31527692","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Modified residue: {"description":"N6-methyllysine; alternate","evidences":[{"source":"PubMed","id":"16267050","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16457588","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17194708","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Modified residue: {"description":"5-glutamyl serotonin; alternate","evidences":[{"source":"PubMed","id":"30867594","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine; by PKC","evidences":[{"source":"PubMed","id":"20228790","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	Modified residue: {"description":"Symmetric dimethylarginine; by PRMT5; alternate","evidences":[{"source":"UniProtKB","id":"P68433","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	Modified residue: {"description":"N6-methyllysine; alternate","evidences":[{"source":"PubMed","id":"11242053","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16185088","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16267050","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16457588","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17194708","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---