Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5N8Y

KaiCBA circadian clock backbone model based on a Cryo-EM density

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0003677	molecular_function	DNA binding
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0006355	biological_process	regulation of DNA-templated transcription
A	0007623	biological_process	circadian rhythm
A	0009649	biological_process	entrainment of circadian clock
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0042752	biological_process	regulation of circadian rhythm
A	0042754	biological_process	negative regulation of circadian rhythm
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
A	0048511	biological_process	rhythmic process
A	0070297	biological_process	regulation of phosphorelay signal transduction system
A	0106310	molecular_function	protein serine kinase activity
B	0000287	molecular_function	magnesium ion binding
B	0003677	molecular_function	DNA binding
B	0004674	molecular_function	protein serine/threonine kinase activity
B	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0006355	biological_process	regulation of DNA-templated transcription
B	0007623	biological_process	circadian rhythm
B	0009649	biological_process	entrainment of circadian clock
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0042752	biological_process	regulation of circadian rhythm
B	0042754	biological_process	negative regulation of circadian rhythm
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding
B	0048511	biological_process	rhythmic process
B	0070297	biological_process	regulation of phosphorelay signal transduction system
B	0106310	molecular_function	protein serine kinase activity
C	0000287	molecular_function	magnesium ion binding
C	0003677	molecular_function	DNA binding
C	0004674	molecular_function	protein serine/threonine kinase activity
C	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0006355	biological_process	regulation of DNA-templated transcription
C	0007623	biological_process	circadian rhythm
C	0009649	biological_process	entrainment of circadian clock
C	0016787	molecular_function	hydrolase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0042752	biological_process	regulation of circadian rhythm
C	0042754	biological_process	negative regulation of circadian rhythm
C	0042802	molecular_function	identical protein binding
C	0046872	molecular_function	metal ion binding
C	0048511	biological_process	rhythmic process
C	0070297	biological_process	regulation of phosphorelay signal transduction system
C	0106310	molecular_function	protein serine kinase activity
D	0000287	molecular_function	magnesium ion binding
D	0003677	molecular_function	DNA binding
D	0004674	molecular_function	protein serine/threonine kinase activity
D	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0006355	biological_process	regulation of DNA-templated transcription
D	0007623	biological_process	circadian rhythm
D	0009649	biological_process	entrainment of circadian clock
D	0016787	molecular_function	hydrolase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0042752	biological_process	regulation of circadian rhythm
D	0042754	biological_process	negative regulation of circadian rhythm
D	0042802	molecular_function	identical protein binding
D	0046872	molecular_function	metal ion binding
D	0048511	biological_process	rhythmic process
D	0070297	biological_process	regulation of phosphorelay signal transduction system
D	0106310	molecular_function	protein serine kinase activity
E	0000287	molecular_function	magnesium ion binding
E	0003677	molecular_function	DNA binding
E	0004674	molecular_function	protein serine/threonine kinase activity
E	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0006355	biological_process	regulation of DNA-templated transcription
E	0007623	biological_process	circadian rhythm
E	0009649	biological_process	entrainment of circadian clock
E	0016787	molecular_function	hydrolase activity
E	0016887	molecular_function	ATP hydrolysis activity
E	0042752	biological_process	regulation of circadian rhythm
E	0042754	biological_process	negative regulation of circadian rhythm
E	0042802	molecular_function	identical protein binding
E	0046872	molecular_function	metal ion binding
E	0048511	biological_process	rhythmic process
E	0070297	biological_process	regulation of phosphorelay signal transduction system
E	0106310	molecular_function	protein serine kinase activity
F	0000287	molecular_function	magnesium ion binding
F	0003677	molecular_function	DNA binding
F	0004674	molecular_function	protein serine/threonine kinase activity
F	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0006355	biological_process	regulation of DNA-templated transcription
F	0007623	biological_process	circadian rhythm
F	0009649	biological_process	entrainment of circadian clock
F	0016787	molecular_function	hydrolase activity
F	0016887	molecular_function	ATP hydrolysis activity
F	0042752	biological_process	regulation of circadian rhythm
F	0042754	biological_process	negative regulation of circadian rhythm
F	0042802	molecular_function	identical protein binding
F	0046872	molecular_function	metal ion binding
F	0048511	biological_process	rhythmic process
F	0070297	biological_process	regulation of phosphorelay signal transduction system
F	0106310	molecular_function	protein serine kinase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005886	cellular_component	plasma membrane
G	0007623	biological_process	circadian rhythm
G	0009649	biological_process	entrainment of circadian clock
G	0042326	biological_process	negative regulation of phosphorylation
G	0042752	biological_process	regulation of circadian rhythm
G	0042802	molecular_function	identical protein binding
G	0048511	biological_process	rhythmic process
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005886	cellular_component	plasma membrane
H	0007623	biological_process	circadian rhythm
H	0009649	biological_process	entrainment of circadian clock
H	0042326	biological_process	negative regulation of phosphorylation
H	0042752	biological_process	regulation of circadian rhythm
H	0042802	molecular_function	identical protein binding
H	0048511	biological_process	rhythmic process
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005886	cellular_component	plasma membrane
I	0007623	biological_process	circadian rhythm
I	0009649	biological_process	entrainment of circadian clock
I	0042326	biological_process	negative regulation of phosphorylation
I	0042752	biological_process	regulation of circadian rhythm
I	0042802	molecular_function	identical protein binding
I	0048511	biological_process	rhythmic process
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0005886	cellular_component	plasma membrane
J	0007623	biological_process	circadian rhythm
J	0009649	biological_process	entrainment of circadian clock
J	0042326	biological_process	negative regulation of phosphorylation
J	0042752	biological_process	regulation of circadian rhythm
J	0042802	molecular_function	identical protein binding
J	0048511	biological_process	rhythmic process
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0005886	cellular_component	plasma membrane
K	0007623	biological_process	circadian rhythm
K	0009649	biological_process	entrainment of circadian clock
K	0042326	biological_process	negative regulation of phosphorylation
K	0042752	biological_process	regulation of circadian rhythm
K	0042802	molecular_function	identical protein binding
K	0048511	biological_process	rhythmic process
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0005886	cellular_component	plasma membrane
L	0007623	biological_process	circadian rhythm
L	0009649	biological_process	entrainment of circadian clock
L	0042326	biological_process	negative regulation of phosphorylation
L	0042752	biological_process	regulation of circadian rhythm
L	0042802	molecular_function	identical protein binding
L	0048511	biological_process	rhythmic process
M	0005515	molecular_function	protein binding
M	0006468	biological_process	protein phosphorylation
M	0007623	biological_process	circadian rhythm
M	0009649	biological_process	entrainment of circadian clock
M	0035308	biological_process	negative regulation of protein dephosphorylation
M	0042753	biological_process	positive regulation of circadian rhythm
M	0042802	molecular_function	identical protein binding
M	0048511	biological_process	rhythmic process
M	0051776	biological_process	detection of redox state
N	0005515	molecular_function	protein binding
N	0006468	biological_process	protein phosphorylation
N	0007623	biological_process	circadian rhythm
N	0009649	biological_process	entrainment of circadian clock
N	0035308	biological_process	negative regulation of protein dephosphorylation
N	0042753	biological_process	positive regulation of circadian rhythm
N	0042802	molecular_function	identical protein binding
N	0048511	biological_process	rhythmic process
N	0051776	biological_process	detection of redox state
O	0005515	molecular_function	protein binding
O	0006468	biological_process	protein phosphorylation
O	0007623	biological_process	circadian rhythm
O	0009649	biological_process	entrainment of circadian clock
O	0035308	biological_process	negative regulation of protein dephosphorylation
O	0042753	biological_process	positive regulation of circadian rhythm
O	0042802	molecular_function	identical protein binding
O	0048511	biological_process	rhythmic process
O	0051776	biological_process	detection of redox state
P	0005515	molecular_function	protein binding
P	0006468	biological_process	protein phosphorylation
P	0007623	biological_process	circadian rhythm
P	0009649	biological_process	entrainment of circadian clock
P	0035308	biological_process	negative regulation of protein dephosphorylation
P	0042753	biological_process	positive regulation of circadian rhythm
P	0042802	molecular_function	identical protein binding
P	0048511	biological_process	rhythmic process
P	0051776	biological_process	detection of redox state
Q	0005515	molecular_function	protein binding
Q	0006468	biological_process	protein phosphorylation
Q	0007623	biological_process	circadian rhythm
Q	0009649	biological_process	entrainment of circadian clock
Q	0035308	biological_process	negative regulation of protein dephosphorylation
Q	0042753	biological_process	positive regulation of circadian rhythm
Q	0042802	molecular_function	identical protein binding
Q	0048511	biological_process	rhythmic process
Q	0051776	biological_process	detection of redox state
R	0005515	molecular_function	protein binding
R	0006468	biological_process	protein phosphorylation
R	0007623	biological_process	circadian rhythm
R	0009649	biological_process	entrainment of circadian clock
R	0035308	biological_process	negative regulation of protein dephosphorylation
R	0042753	biological_process	positive regulation of circadian rhythm
R	0042802	molecular_function	identical protein binding
R	0048511	biological_process	rhythmic process
R	0051776	biological_process	detection of redox state
S	0005515	molecular_function	protein binding
S	0006468	biological_process	protein phosphorylation
S	0007623	biological_process	circadian rhythm
S	0009649	biological_process	entrainment of circadian clock
S	0035308	biological_process	negative regulation of protein dephosphorylation
S	0042753	biological_process	positive regulation of circadian rhythm
S	0042802	molecular_function	identical protein binding
S	0048511	biological_process	rhythmic process
S	0051776	biological_process	detection of redox state
T	0005515	molecular_function	protein binding
T	0006468	biological_process	protein phosphorylation
T	0007623	biological_process	circadian rhythm
T	0009649	biological_process	entrainment of circadian clock
T	0035308	biological_process	negative regulation of protein dephosphorylation
T	0042753	biological_process	positive regulation of circadian rhythm
T	0042802	molecular_function	identical protein binding
T	0048511	biological_process	rhythmic process
T	0051776	biological_process	detection of redox state
U	0005515	molecular_function	protein binding
U	0006468	biological_process	protein phosphorylation
U	0007623	biological_process	circadian rhythm
U	0009649	biological_process	entrainment of circadian clock
U	0035308	biological_process	negative regulation of protein dephosphorylation
U	0042753	biological_process	positive regulation of circadian rhythm
U	0042802	molecular_function	identical protein binding
U	0048511	biological_process	rhythmic process
U	0051776	biological_process	detection of redox state
V	0005515	molecular_function	protein binding
V	0006468	biological_process	protein phosphorylation
V	0007623	biological_process	circadian rhythm
V	0009649	biological_process	entrainment of circadian clock
V	0035308	biological_process	negative regulation of protein dephosphorylation
V	0042753	biological_process	positive regulation of circadian rhythm
V	0042802	molecular_function	identical protein binding
V	0048511	biological_process	rhythmic process
V	0051776	biological_process	detection of redox state
W	0005515	molecular_function	protein binding
W	0006468	biological_process	protein phosphorylation
W	0007623	biological_process	circadian rhythm
W	0009649	biological_process	entrainment of circadian clock
W	0035308	biological_process	negative regulation of protein dephosphorylation
W	0042753	biological_process	positive regulation of circadian rhythm
W	0042802	molecular_function	identical protein binding
W	0048511	biological_process	rhythmic process
W	0051776	biological_process	detection of redox state
X	0005515	molecular_function	protein binding
X	0006468	biological_process	protein phosphorylation
X	0007623	biological_process	circadian rhythm
X	0009649	biological_process	entrainment of circadian clock
X	0035308	biological_process	negative regulation of protein dephosphorylation
X	0042753	biological_process	positive regulation of circadian rhythm
X	0042802	molecular_function	identical protein binding
X	0048511	biological_process	rhythmic process
X	0051776	biological_process	detection of redox state

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	ACT_SITE: Proton acceptor in CI (KaiC 1) => ECO:0000305\|PubMed:22304631

Chain	Residue	Details
A	GLU77
B	GLU77
C	GLU77
D	GLU77
E	GLU77
F	GLU77

site_id	SWS_FT_FI2
Number of Residues	6
Details	ACT_SITE: Proton acceptor in CII (KaiC 2) => ECO:0000305\|PubMed:22304631

Chain	Residue	Details
A	GLU318
B	GLU318
C	GLU318
D	GLU318
E	GLU318
F	GLU318

site_id	SWS_FT_FI3
Number of Residues	150
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_01836, ECO:0000269\|PubMed:15304218, ECO:0000269\|PubMed:16628225, ECO:0000269\|PubMed:22304631, ECO:0007744\|PDB:1TF7, ECO:0007744\|PDB:2GBL, ECO:0007744\|PDB:4DUG

Chain	Residue	Details
A	GLY49
A	HIS230
D	LYS465
E	GLY49
E	THR50
E	GLY51
E	LYS52
E	LEU54
E	SER89
E	LYS224
E	ARG226
E	THR228
A	THR240
E	HIS230
E	THR240
E	ASP241
E	THR290
E	GLY291
E	THR292
E	GLY293
E	LYS294
E	LEU296
E	TRP331
A	ASP241
E	ARG451
E	LYS457
E	ARG459
E	SER461
E	HIS463
E	LYS465
F	GLY49
F	THR50
F	GLY51
F	LYS52
A	THR290
F	LEU54
F	SER89
F	LYS224
F	ARG226
F	THR228
F	HIS230
F	THR240
F	ASP241
F	THR290
F	GLY291
A	GLY291
F	THR292
F	GLY293
F	LYS294
F	LEU296
F	TRP331
F	ARG451
F	LYS457
F	ARG459
F	SER461
F	HIS463
A	THR292
F	LYS465
A	GLY293
A	LYS294
A	LEU296
A	TRP331
A	THR50
A	ARG451
A	LYS457
A	ARG459
A	SER461
A	HIS463
A	LYS465
B	GLY49
B	THR50
B	GLY51
B	LYS52
A	GLY51
B	LEU54
B	SER89
B	LYS224
B	ARG226
B	THR228
B	HIS230
B	THR240
B	ASP241
B	THR290
B	GLY291
A	LYS52
B	THR292
B	GLY293
B	LYS294
B	LEU296
B	TRP331
B	ARG451
B	LYS457
B	ARG459
B	SER461
B	HIS463
A	LEU54
B	LYS465
C	GLY49
C	THR50
C	GLY51
C	LYS52
C	LEU54
C	SER89
C	LYS224
C	ARG226
C	THR228
A	SER89
C	HIS230
C	THR240
C	ASP241
C	THR290
C	GLY291
C	THR292
C	GLY293
C	LYS294
C	LEU296
C	TRP331
A	LYS224
C	ARG451
C	LYS457
C	ARG459
C	SER461
C	HIS463
C	LYS465
D	GLY49
D	THR50
D	GLY51
D	LYS52
A	ARG226
D	LEU54
D	SER89
D	LYS224
D	ARG226
D	THR228
D	HIS230
D	THR240
D	ASP241
D	THR290
D	GLY291
A	THR228
D	THR292
D	GLY293
D	LYS294
D	LEU296
D	TRP331
D	ARG451
D	LYS457
D	ARG459
D	SER461
D	HIS463

site_id	SWS_FT_FI4
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_01836, ECO:0007744\|PDB:4DUG, ECO:0007744\|PDB:4TL6, ECO:0007744\|PDB:7DXQ

Chain	Residue	Details
A	THR53
B	THR53
C	THR53
D	THR53
E	THR53
F	THR53

site_id	SWS_FT_FI5
Number of Residues	12
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_01836, ECO:0000269\|PubMed:15304218, ECO:0000269\|PubMed:16628225, ECO:0007744\|PDB:1TF7, ECO:0007744\|PDB:2GBL

Chain	Residue	Details
A	LEU225
E	MET458
F	LEU225
F	MET458
A	MET458
B	LEU225
B	MET458
C	LEU225
C	MET458
D	LEU225
D	MET458
E	LEU225

site_id	SWS_FT_FI6
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_01836, ECO:0000269\|PubMed:15304218, ECO:0000269\|PubMed:16628225, ECO:0007744\|PDB:1U9I, ECO:0007744\|PDB:2GBL, ECO:0007744\|PDB:4DUG, ECO:0007744\|PDB:7DXQ

Chain	Residue	Details
A	THR295
B	THR295
C	THR295
D	THR295
E	THR295
F	THR295

site_id	SWS_FT_FI7
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_01836, ECO:0000269\|PubMed:15304218, ECO:0000269\|PubMed:16628225

Chain	Residue	Details
A	GLU318
B	GLU318
C	GLU318
D	GLU318
E	GLU318
F	GLU318

site_id	SWS_FT_FI8
Number of Residues	6
Details	MOD_RES: Phosphoserine; by autocatalysis => ECO:0000255\|HAMAP-Rule:MF_01836, ECO:0000269\|PubMed:15347809, ECO:0000269\|PubMed:15347812, ECO:0000269\|PubMed:17717528, ECO:0000269\|PubMed:17916691, ECO:0007744\|PDB:1U9I, ECO:0007744\|PDB:2GBL

Chain	Residue	Details
A	SER431
B	SER431
C	SER431
D	SER431
E	SER431
F	SER431

site_id	SWS_FT_FI9
Number of Residues	6
Details	MOD_RES: Phosphothreonine; by autocatalysis => ECO:0000255\|HAMAP-Rule:MF_01836, ECO:0000269\|PubMed:15304218, ECO:0000269\|PubMed:15347809, ECO:0000269\|PubMed:15347812, ECO:0000269\|PubMed:17717528, ECO:0000269\|PubMed:17916691, ECO:0007744\|PDB:1U9I, ECO:0007744\|PDB:2GBL

Chain	Residue	Details
A	THR432
B	THR432
C	THR432
D	THR432
E	THR432
F	THR432

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)