5N8Y

KaiCBA circadian clock backbone model based on a Cryo-EM density

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0003677	molecular_function	DNA binding
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0006351	biological_process	DNA-templated transcription
A	0006355	biological_process	regulation of DNA-templated transcription
A	0007623	biological_process	circadian rhythm
A	0009649	biological_process	entrainment of circadian clock
A	0016301	molecular_function	kinase activity
A	0016740	molecular_function	transferase activity
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0042752	biological_process	regulation of circadian rhythm
A	0042754	biological_process	negative regulation of circadian rhythm
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
A	0048511	biological_process	rhythmic process
A	0070297	biological_process	regulation of phosphorelay signal transduction system
A	0106310	molecular_function	protein serine kinase activity
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0003677	molecular_function	DNA binding
B	0004674	molecular_function	protein serine/threonine kinase activity
B	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0006351	biological_process	DNA-templated transcription
B	0006355	biological_process	regulation of DNA-templated transcription
B	0007623	biological_process	circadian rhythm
B	0009649	biological_process	entrainment of circadian clock
B	0016301	molecular_function	kinase activity
B	0016740	molecular_function	transferase activity
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0042752	biological_process	regulation of circadian rhythm
B	0042754	biological_process	negative regulation of circadian rhythm
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding
B	0048511	biological_process	rhythmic process
B	0070297	biological_process	regulation of phosphorelay signal transduction system
B	0106310	molecular_function	protein serine kinase activity
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0003677	molecular_function	DNA binding
C	0004674	molecular_function	protein serine/threonine kinase activity
C	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0006351	biological_process	DNA-templated transcription
C	0006355	biological_process	regulation of DNA-templated transcription
C	0007623	biological_process	circadian rhythm
C	0009649	biological_process	entrainment of circadian clock
C	0016301	molecular_function	kinase activity
C	0016740	molecular_function	transferase activity
C	0016787	molecular_function	hydrolase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0042752	biological_process	regulation of circadian rhythm
C	0042754	biological_process	negative regulation of circadian rhythm
C	0042802	molecular_function	identical protein binding
C	0046872	molecular_function	metal ion binding
C	0048511	biological_process	rhythmic process
C	0070297	biological_process	regulation of phosphorelay signal transduction system
C	0106310	molecular_function	protein serine kinase activity
D	0000166	molecular_function	nucleotide binding
D	0000287	molecular_function	magnesium ion binding
D	0003677	molecular_function	DNA binding
D	0004674	molecular_function	protein serine/threonine kinase activity
D	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0006351	biological_process	DNA-templated transcription
D	0006355	biological_process	regulation of DNA-templated transcription
D	0007623	biological_process	circadian rhythm
D	0009649	biological_process	entrainment of circadian clock
D	0016301	molecular_function	kinase activity
D	0016740	molecular_function	transferase activity
D	0016787	molecular_function	hydrolase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0042752	biological_process	regulation of circadian rhythm
D	0042754	biological_process	negative regulation of circadian rhythm
D	0042802	molecular_function	identical protein binding
D	0046872	molecular_function	metal ion binding
D	0048511	biological_process	rhythmic process
D	0070297	biological_process	regulation of phosphorelay signal transduction system
D	0106310	molecular_function	protein serine kinase activity
E	0000166	molecular_function	nucleotide binding
E	0000287	molecular_function	magnesium ion binding
E	0003677	molecular_function	DNA binding
E	0004674	molecular_function	protein serine/threonine kinase activity
E	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0006351	biological_process	DNA-templated transcription
E	0006355	biological_process	regulation of DNA-templated transcription
E	0007623	biological_process	circadian rhythm
E	0009649	biological_process	entrainment of circadian clock
E	0016301	molecular_function	kinase activity
E	0016740	molecular_function	transferase activity
E	0016787	molecular_function	hydrolase activity
E	0016887	molecular_function	ATP hydrolysis activity
E	0042752	biological_process	regulation of circadian rhythm
E	0042754	biological_process	negative regulation of circadian rhythm
E	0042802	molecular_function	identical protein binding
E	0046872	molecular_function	metal ion binding
E	0048511	biological_process	rhythmic process
E	0070297	biological_process	regulation of phosphorelay signal transduction system
E	0106310	molecular_function	protein serine kinase activity
F	0000166	molecular_function	nucleotide binding
F	0000287	molecular_function	magnesium ion binding
F	0003677	molecular_function	DNA binding
F	0004674	molecular_function	protein serine/threonine kinase activity
F	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0006351	biological_process	DNA-templated transcription
F	0006355	biological_process	regulation of DNA-templated transcription
F	0007623	biological_process	circadian rhythm
F	0009649	biological_process	entrainment of circadian clock
F	0016301	molecular_function	kinase activity
F	0016740	molecular_function	transferase activity
F	0016787	molecular_function	hydrolase activity
F	0016887	molecular_function	ATP hydrolysis activity
F	0042752	biological_process	regulation of circadian rhythm
F	0042754	biological_process	negative regulation of circadian rhythm
F	0042802	molecular_function	identical protein binding
F	0046872	molecular_function	metal ion binding
F	0048511	biological_process	rhythmic process
F	0070297	biological_process	regulation of phosphorelay signal transduction system
F	0106310	molecular_function	protein serine kinase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0005886	cellular_component	plasma membrane
G	0007623	biological_process	circadian rhythm
G	0009649	biological_process	entrainment of circadian clock
G	0042326	biological_process	negative regulation of phosphorylation
G	0042752	biological_process	regulation of circadian rhythm
G	0042802	molecular_function	identical protein binding
G	0048511	biological_process	rhythmic process
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005886	cellular_component	plasma membrane
H	0007623	biological_process	circadian rhythm
H	0009649	biological_process	entrainment of circadian clock
H	0042326	biological_process	negative regulation of phosphorylation
H	0042752	biological_process	regulation of circadian rhythm
H	0042802	molecular_function	identical protein binding
H	0048511	biological_process	rhythmic process
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005886	cellular_component	plasma membrane
I	0007623	biological_process	circadian rhythm
I	0009649	biological_process	entrainment of circadian clock
I	0042326	biological_process	negative regulation of phosphorylation
I	0042752	biological_process	regulation of circadian rhythm
I	0042802	molecular_function	identical protein binding
I	0048511	biological_process	rhythmic process
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0005886	cellular_component	plasma membrane
J	0007623	biological_process	circadian rhythm
J	0009649	biological_process	entrainment of circadian clock
J	0042326	biological_process	negative regulation of phosphorylation
J	0042752	biological_process	regulation of circadian rhythm
J	0042802	molecular_function	identical protein binding
J	0048511	biological_process	rhythmic process
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0005886	cellular_component	plasma membrane
K	0007623	biological_process	circadian rhythm
K	0009649	biological_process	entrainment of circadian clock
K	0042326	biological_process	negative regulation of phosphorylation
K	0042752	biological_process	regulation of circadian rhythm
K	0042802	molecular_function	identical protein binding
K	0048511	biological_process	rhythmic process
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0005886	cellular_component	plasma membrane
L	0007623	biological_process	circadian rhythm
L	0009649	biological_process	entrainment of circadian clock
L	0042326	biological_process	negative regulation of phosphorylation
L	0042752	biological_process	regulation of circadian rhythm
L	0042802	molecular_function	identical protein binding
L	0048511	biological_process	rhythmic process
M	0005515	molecular_function	protein binding
M	0006468	biological_process	protein phosphorylation
M	0007623	biological_process	circadian rhythm
M	0009649	biological_process	entrainment of circadian clock
M	0042753	biological_process	positive regulation of circadian rhythm
M	0042802	molecular_function	identical protein binding
M	0048511	biological_process	rhythmic process
M	0051776	biological_process	detection of redox state
N	0005515	molecular_function	protein binding
N	0006468	biological_process	protein phosphorylation
N	0007623	biological_process	circadian rhythm
N	0009649	biological_process	entrainment of circadian clock
N	0042753	biological_process	positive regulation of circadian rhythm
N	0042802	molecular_function	identical protein binding
N	0048511	biological_process	rhythmic process
N	0051776	biological_process	detection of redox state
O	0005515	molecular_function	protein binding
O	0006468	biological_process	protein phosphorylation
O	0007623	biological_process	circadian rhythm
O	0009649	biological_process	entrainment of circadian clock
O	0042753	biological_process	positive regulation of circadian rhythm
O	0042802	molecular_function	identical protein binding
O	0048511	biological_process	rhythmic process
O	0051776	biological_process	detection of redox state
P	0005515	molecular_function	protein binding
P	0006468	biological_process	protein phosphorylation
P	0007623	biological_process	circadian rhythm
P	0009649	biological_process	entrainment of circadian clock
P	0042753	biological_process	positive regulation of circadian rhythm
P	0042802	molecular_function	identical protein binding
P	0048511	biological_process	rhythmic process
P	0051776	biological_process	detection of redox state
Q	0005515	molecular_function	protein binding
Q	0006468	biological_process	protein phosphorylation
Q	0007623	biological_process	circadian rhythm
Q	0009649	biological_process	entrainment of circadian clock
Q	0042753	biological_process	positive regulation of circadian rhythm
Q	0042802	molecular_function	identical protein binding
Q	0048511	biological_process	rhythmic process
Q	0051776	biological_process	detection of redox state
R	0005515	molecular_function	protein binding
R	0006468	biological_process	protein phosphorylation
R	0007623	biological_process	circadian rhythm
R	0009649	biological_process	entrainment of circadian clock
R	0042753	biological_process	positive regulation of circadian rhythm
R	0042802	molecular_function	identical protein binding
R	0048511	biological_process	rhythmic process
R	0051776	biological_process	detection of redox state
S	0005515	molecular_function	protein binding
S	0006468	biological_process	protein phosphorylation
S	0007623	biological_process	circadian rhythm
S	0009649	biological_process	entrainment of circadian clock
S	0042753	biological_process	positive regulation of circadian rhythm
S	0042802	molecular_function	identical protein binding
S	0048511	biological_process	rhythmic process
S	0051776	biological_process	detection of redox state
T	0005515	molecular_function	protein binding
T	0006468	biological_process	protein phosphorylation
T	0007623	biological_process	circadian rhythm
T	0009649	biological_process	entrainment of circadian clock
T	0042753	biological_process	positive regulation of circadian rhythm
T	0042802	molecular_function	identical protein binding
T	0048511	biological_process	rhythmic process
T	0051776	biological_process	detection of redox state
U	0005515	molecular_function	protein binding
U	0006468	biological_process	protein phosphorylation
U	0007623	biological_process	circadian rhythm
U	0009649	biological_process	entrainment of circadian clock
U	0042753	biological_process	positive regulation of circadian rhythm
U	0042802	molecular_function	identical protein binding
U	0048511	biological_process	rhythmic process
U	0051776	biological_process	detection of redox state
V	0005515	molecular_function	protein binding
V	0006468	biological_process	protein phosphorylation
V	0007623	biological_process	circadian rhythm
V	0009649	biological_process	entrainment of circadian clock
V	0042753	biological_process	positive regulation of circadian rhythm
V	0042802	molecular_function	identical protein binding
V	0048511	biological_process	rhythmic process
V	0051776	biological_process	detection of redox state
W	0005515	molecular_function	protein binding
W	0006468	biological_process	protein phosphorylation
W	0007623	biological_process	circadian rhythm
W	0009649	biological_process	entrainment of circadian clock
W	0042753	biological_process	positive regulation of circadian rhythm
W	0042802	molecular_function	identical protein binding
W	0048511	biological_process	rhythmic process
W	0051776	biological_process	detection of redox state
X	0005515	molecular_function	protein binding
X	0006468	biological_process	protein phosphorylation
X	0007623	biological_process	circadian rhythm
X	0009649	biological_process	entrainment of circadian clock
X	0042753	biological_process	positive regulation of circadian rhythm
X	0042802	molecular_function	identical protein binding
X	0048511	biological_process	rhythmic process
X	0051776	biological_process	detection of redox state

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	42
Details	Region: {"description":"B-loop, required to bind KaiB and SasA","evidences":[{"source":"UniProtKB","id":"Q79V60","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	72
Details	Region: {"description":"Linker","evidences":[{"source":"PubMed","id":"15304218","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	6
Details	Active site: {"description":"Proton acceptor in CI (KaiC 1)","evidences":[{"source":"PubMed","id":"22304631","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	Active site: {"description":"Proton acceptor in CII (KaiC 2)","evidences":[{"source":"PubMed","id":"22304631","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	150
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01836","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15304218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16628225","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22304631","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1TF7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2GBL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4DUG","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01836","evidenceCode":"ECO:0000255"},{"source":"PDB","id":"4DUG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4TL6","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7DXQ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01836","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15304218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16628225","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1TF7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2GBL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01836","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15304218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16628225","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1U9I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2GBL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4DUG","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7DXQ","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01836","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15304218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16628225","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine; by autocatalysis","evidences":[{"source":"HAMAP-Rule","id":"MF_01836","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15347809","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15347812","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17717528","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17916691","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1U9I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2GBL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	6
Details	Modified residue: {"description":"Phosphothreonine; by autocatalysis","evidences":[{"source":"HAMAP-Rule","id":"MF_01836","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15304218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15347809","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"15347812","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17717528","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"17916691","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1U9I","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2GBL","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	648
Details	Domain: {"description":"KaiA C-terminal","evidences":[{"source":"PROSITE-ProRule","id":"PRU00761","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"15007067","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---